NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8642R-1

Symbol

CG8642

Full Name

CG8642

CG No

CG8642

Old CG No

CG8642

Synonyms

CG8642

Accession No (Link to NCBI)

NM_136552.1

Inserted Chr.

lll

Insertional Mutation

2 lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

pupal lethal

Map Viewer

Reference
[Please submit your publication]

Graca FA, Rai M, Hunt LC, Stephan A, Wang YD, Gordon B, Wang R, Quarato G, Xu B, Fan Y, Labelle M, Demontis F.<br> The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.<br> Nat Commun (2022) 13(1) 2370 [ PubMed ID = 35501350 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAGAGTGCCGCCACGATTAAG

Primer Seq. 3'

aatctagaggtaccACAGATCCATCCAGACGCCGC

Predicted Fragment Size

IR fragment full Seq

0001 GAGAGTGCCG CCACGATTAA GGACAAGGAG AACGAGATAA ACAAGCTGCA GACCAAGGAC 
0061 CAAACGGGCG ATGAgNTGCT GAGGAAATTC GAGAATCTCA CCCAGGTGTG CAGTGCCCAG 
0121 CAGTCGTCGT TCACGACGGC CATTAAAGAG AAGGACGAAC AGATCAAGGA ACTGGAGCAA 
0181 AAGGTGAAGG TGTACGAGAC GCGCCTCAAG AGGAAACAGC ACGTTCTGGC GGAGCTGGCG 
0241 CAAGCTGAAT GGCTCCAGTG CTCTACTGAT CGAGCATCTC AAGGGTAAGG TTGTGTACTT 
0301 CGAGCGGAAG TTTCGGGAGA AGAAGGACGA CCTGTTGGCG GACTGGGAGG CAGCGACTAC 
0361 CAGCTGCGTG CCCTTTGGAC GATCGCCCGG CATCCATCTG ATCCATCTGC CCGGATTCCT 
0421 GCCCTTCTTG GTGcCCTGCG AGGGGCAAAC TGCCGcCGGA CCCgGCTGGA CTTGCATCCA 
0481 GCGGCGTCTG GATGGATCTG T

in silico PCR Fragment

0001 GAGAGTGCCG CCACGATTAA GGACAAGGAG AACGAGATAA ACAAGCTGCA GACCAAGGAC 
0061 CAAACGGGCG ATGAGCTGCT GAGGAAATTC GAGAATCTCA CCCAGGTGTG CAGTGCCCAG 
0121 CAGTCGTCGT TCACGACGGC CATTAAAGAG AAGGACGAAC AGATCAAGGA ACTGGAGCAA 
0181 AAGGTGAAGG TGTACGAGAC GCGCCTCAAG AGGAAACAGC ACGTTCTGGC GGAGCT-GCG 
0241 CAAGCTGAAT GGCTCCAGTG CTCTACTGAT CGAGCATCTC AAGGGTAAGG TTGTGTACTT 
0301 CGAGCGGAAG TTTCGGGAGA AGAAGGACGA CCTGTTGGCG GACTGGGAGG CAGCGACTAC 
0361 CAGCTGCGTG CCCTTTGGAC GATCGCCCGG CATCCATCTG ATCCATCTGC CCGGATTCCT 
0421 GCCCTTCTTG GTGCCCTGCG AGGGGCAAAC TGCCGCCGGA CCCGGCTGGA CTTGCATCCA 
0481 GCGGCGTCTG GATGGATCTG T

Assemble Data

<PRE>

8642R-1.IR_full       1   GAGAGTGCCGCCACGATTAAGGACAAGGAGAACGAGATAAACAAGCTGCAGACCAAGGAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8642R-1.in silico     1   GAGAGTGCCGCCACGATTAAGGACAAGGAGAACGAGATAAACAAGCTGCAGACCAAGGAC 60


8642R-1.IR_full       61  CAAACGGGCGATGAG<span class="snp"><tt>N</tt></span>TGCTGAGGAAATTCGAGAATCTCACCCAGGTGTGCAGTGCCCAG 120
                          |||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||
8642R-1.in silico     61  CAAACGGGCGATGAG<span class="snp"><tt>C</tt></span>TGCTGAGGAAATTCGAGAATCTCACCCAGGTGTGCAGTGCCCAG 120


8642R-1.IR_full       121 CAGTCGTCGTTCACGACGGCCATTAAAGAGAAGGACGAACAGATCAAGGAACTGGAGCAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8642R-1.in silico     121 CAGTCGTCGTTCACGACGGCCATTAAAGAGAAGGACGAACAGATCAAGGAACTGGAGCAA 180


8642R-1.IR_full       181 AAGGTGAAGGTGTACGAGACGCGCCTCAAGAGGAAACAGCACGTTCTGGCGGAGCT<span class="snp"><tt>G</tt></span>GCG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||
8642R-1.in silico     181 AAGGTGAAGGTGTACGAGACGCGCCTCAAGAGGAAACAGCACGTTCTGGCGGAGCT<span class="snp"><tt>-</tt></span>GCG 240


8642R-1.IR_full       241 CAAGCTGAATGGCTCCAGTGCTCTACTGATCGAGCATCTCAAGGGTAAGGTTGTGTACTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8642R-1.in silico     241 CAAGCTGAATGGCTCCAGTGCTCTACTGATCGAGCATCTCAAGGGTAAGGTTGTGTACTT 300


8642R-1.IR_full       301 CGAGCGGAAGTTTCGGGAGAAGAAGGACGACCTGTTGGCGGACTGGGAGGCAGCGACTAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8642R-1.in silico     301 CGAGCGGAAGTTTCGGGAGAAGAAGGACGACCTGTTGGCGGACTGGGAGGCAGCGACTAC 360


8642R-1.IR_full       361 CAGCTGCGTGCCCTTTGGACGATCGCCCGGCATCCATCTGATCCATCTGCCCGGATTCCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8642R-1.in silico     361 CAGCTGCGTGCCCTTTGGACGATCGCCCGGCATCCATCTGATCCATCTGCCCGGATTCCT 420


8642R-1.IR_full       421 GCCCTTCTTGGTGCCCTGCGAGGGGCAAACTGCCGCCGGACCCGGCTGGACTTGCATCCA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8642R-1.in silico     421 GCCCTTCTTGGTGCCCTGCGAGGGGCAAACTGCCGCCGGACCCGGCTGGACTTGCATCCA 480


8642R-1.IR_full       481 GCGGCGTCTGGATGGATCTGT 501
                          |||||||||||||||||||||
8642R-1.in silico     481 GCGGCGTCTGGATGGATCTGT 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136552.1	CG8642-RA (CG8642), mRNA
0.2	1	2	2	4	NM_134832.2	CG15390-RA (CG15390), mRNA
0	0	8	2	2	NM_139689.2	CG10672-RA (CG10672), mRNA
0	0	4	3	4	NM_132130.1	CG12796-RA (CG12796), mRNA
0	0	3	2	10	NM_166019.1	CG18076-RH, transcript variant H (shot), mRNA
0	0	3	2	9	NM_166016.1	CG18076-RB, transcript variant B (shot), mRNA
0	0	3	2	9	NM_079009.2	CG18076-RA, transcript variant A (shot), mRNA
0	0	3	2	9	NM_166015.1	CG18076-RG, transcript variant G (shot), mRNA
0	0	2	4	7	NM_138229.1	CG13908-RA (CG13908), mRNA
0	0	2	3	2	NM_139648.1	CG15022-RA (CG15022), mRNA
0	0	2	3	2	NM_133164.2	CG10142-RA (Ance-5), mRNA
0	0	2	2	5	NM_138031.1	CG3173-RA (CG3173), mRNA
0	0	2	2	3	NM_134544.1	CG11734-RB (HERC2), mRNA
0	0	2	2	2	NM_165906.1	CG12369-RB, transcript variant B (Lac), mRNA
0	0	2	2	2	NM_166247.1	CG6406-RA, transcript variant A (CG6406), mRNA
0	0	2	2	2	NM_137412.2	CG6406-RB, transcript variant B (CG6406), mRNA
0	0	2	2	2	NM_078989.2	CG12369-RA, transcript variant A (Lac), mRNA
0	0	1	7	17	NM_001043070.1	CG34146-RA (brp), mRNA
0	0	1	6	2	NM_139453.1	CG13804-RA (yellow-g2), mRNA
0	0	1	5	15	NM_057391.3	CG1977-RA (alpha-Spec), mRNA
0	0	1	4	3	NM_078562.2	CG1639-RA (l(1)10Bb), mRNA
0	0	1	4	3	NM_137815.2	CG3380-RA (Oatp58Dc), mRNA
0	0	1	3	9	NM_132013.2	CG3187-RC, transcript variant C (Sirt4), mRNA
0	0	1	3	9	NM_167041.1	CG3187-RA, transcript variant A (Sirt4), mRNA
0	0	1	3	9	NM_167042.2	CG3187-RB, transcript variant B (Sirt4), mRNA
0	0	1	3	7	NM_132578.1	CG11146-RA (CG11146), mRNA
0	0	1	2	6	NM_166909.2	CG14814-RA, transcript variant A (CG14814), mRNA
0	0	1	2	6	NM_130591.2	CG14814-RB, transcript variant B (CG14814), mRNA
0	0	1	2	4	NM_140893.2	CG15881-RB, transcript variant B (CG15881), mRNA
0	0	1	2	4	NM_141813.2	CG6728-RA (ninaG), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

Back