NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8606R-1

Symbol

RhoGEF4

Full Name

RhoGEF4

CG No

CG8606

Old CG No

CG8606

Synonyms

CG8606, RhoGEF4, DRhoGEF4

Accession No (Link to NCBI)

NM_139844.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCTTCATTCACTCGCCACAAA

Primer Seq. 3'

aatctagaggtaccTCGGAACACCGGATTCTTGCT

Predicted Fragment Size

IR fragment full Seq

0001 GCTTCATTCA CTCGCCACAA ACACCTCTCA GCTTCAGCCG GGAACTGCGG CAGGTGCTGC 
0061 AGGAGCGGAA TCTTCTAACT GCCAAGTCGC GTAGGAAAGT TTGCTCCATG CTGGACAGCA 
0121 ACAACAGCAG TCCGATTGGC TCCCCCAATC CGGACGCGGG AAAAAGATTG GGTTTCCGTC 
0181 GCCAGGCCAT CCAGGAGATT ATTTCGTCGG AGAAATCGTA TCTGGAGCAG CTGGAGCTGC 
0241 TGATGAACTT TTTTGTGCGT CCCTTGAAGG AACAGGCTAT CATCGATTGC TCCAATCACA 
0301 CGCTGCTCTT CGGTCAAATC GAGATGATTC ACAATCTTAA CGGAGAATTC CTGCGCGAAC 
0361 TGGAGGCGAA CATGGAGAAT GTTGCGCACG CATTCCTGAA AATGGCTCCT TTCTTCAAAC 
0421 TGTACTCGGT GTACGCCTTT GACTACCGCG GAGCTCTGTT CATCATCCAG GATTTGATCA 
0481 GCAAGAATCC GGTGTTCCGA

in silico PCR Fragment

0001 GCTTCATTCA CTCGCCACAA ACACCTCTCA GCTTCAGCCG GGAACTGCGG CAGGTGCTGC 
0061 AGGAGCGGAA TCTTCTAACT GCCAAGTCGC GTAGGAAAGT TTGCTCCATG CTGGACAGCA 
0121 ACAACAGCAG TCCGATTGGC TCCCCCAATC CGGACGCGGG AAAAAGATTG GGTTTCCGTC 
0181 GCCAGGCCAT CCAGGAGATT ATTTCGTCGG AGAAATCGTA TCTGGAGCAG CTGGAGCTGC 
0241 TGATGAACTT TTTTGTGCGT CCCTTGAAGG AACAGGCTAT CATCGATTGC TCCAATCACA 
0301 CGCTGCTCTT CGGTCAAATC GAGATGATTC ACAATCTTAA CGGAGAATTC CTGCGCGAAC 
0361 TGGAGGCGAA CATGGAGAAT GTTGCGCACG CATTCCTGAA AATGGCTCCT TTCTTCAAAC 
0421 TGTACTCGGT GTACGCCTTT GACTACCGCG GAGCTCTGTT CATCATCCAG GATTTGATCA 
0481 GCAAGAATCC GGTGTTCCGA

Assemble Data

<PRE>

8606R-1.IR_full       1   GCTTCATTCACTCGCCACAAACACCTCTCAGCTTCAGCCGGGAACTGCGGCAGGTGCTGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8606R-1.in silico     1   GCTTCATTCACTCGCCACAAACACCTCTCAGCTTCAGCCGGGAACTGCGGCAGGTGCTGC 60


8606R-1.IR_full       61  AGGAGCGGAATCTTCTAACTGCCAAGTCGCGTAGGAAAGTTTGCTCCATGCTGGACAGCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8606R-1.in silico     61  AGGAGCGGAATCTTCTAACTGCCAAGTCGCGTAGGAAAGTTTGCTCCATGCTGGACAGCA 120


8606R-1.IR_full       121 ACAACAGCAGTCCGATTGGCTCCCCCAATCCGGACGCGGGAAAAAGATTGGGTTTCCGTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8606R-1.in silico     121 ACAACAGCAGTCCGATTGGCTCCCCCAATCCGGACGCGGGAAAAAGATTGGGTTTCCGTC 180


8606R-1.IR_full       181 GCCAGGCCATCCAGGAGATTATTTCGTCGGAGAAATCGTATCTGGAGCAGCTGGAGCTGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8606R-1.in silico     181 GCCAGGCCATCCAGGAGATTATTTCGTCGGAGAAATCGTATCTGGAGCAGCTGGAGCTGC 240


8606R-1.IR_full       241 TGATGAACTTTTTTGTGCGTCCCTTGAAGGAACAGGCTATCATCGATTGCTCCAATCACA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8606R-1.in silico     241 TGATGAACTTTTTTGTGCGTCCCTTGAAGGAACAGGCTATCATCGATTGCTCCAATCACA 300


8606R-1.IR_full       301 CGCTGCTCTTCGGTCAAATCGAGATGATTCACAATCTTAACGGAGAATTCCTGCGCGAAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8606R-1.in silico     301 CGCTGCTCTTCGGTCAAATCGAGATGATTCACAATCTTAACGGAGAATTCCTGCGCGAAC 360


8606R-1.IR_full       361 TGGAGGCGAACATGGAGAATGTTGCGCACGCATTCCTGAAAATGGCTCCTTTCTTCAAAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8606R-1.in silico     361 TGGAGGCGAACATGGAGAATGTTGCGCACGCATTCCTGAAAATGGCTCCTTTCTTCAAAC 420


8606R-1.IR_full       421 TGTACTCGGTGTACGCCTTTGACTACCGCGGAGCTCTGTTCATCATCCAGGATTTGATCA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8606R-1.in silico     421 TGTACTCGGTGTACGCCTTTGACTACCGCGGAGCTCTGTTCATCATCCAGGATTTGATCA 480


8606R-1.IR_full       481 GCAAGAATCCGGTGTTCCGA 500
                          ||||||||||||||||||||
8606R-1.in silico     481 GCAAGAATCCGGTGTTCCGA 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139844.2	CG8606-RA, transcript variant A (RhoGEF4), mRNA
79.87	385	1	1	1	NM_168199.1	CG8606-RB, transcript variant B (RhoGEF4), mRNA
0.2	1	3	3	3	NM_139366.2	CG12099-RB, transcript variant B (CG12099), mRNA
0.2	1	3	3	3	NM_167895.1	CG12099-RA, transcript variant A (CG12099), mRNA
0	0	4	3	3	NM_165685.1	CG30338-RA (CG30338), mRNA
0	0	3	6	23	NM_080167.3	CG9074-RA (Mst57Da), mRNA
0	0	3	5	4	NM_078722.2	CG17941-RA (ds), mRNA
0	0	3	3	4	NM_080162.3	CG9900-RB, transcript variant B (mit(1)15), mRNA
0	0	3	3	4	NM_001038739.1	CG9900-RC, transcript variant C (mit(1)15), mRNA
0	0	3	2	17	NM_132412.1	CG9817-RA (CG9817), mRNA
0	0	2	11	22	NM_137342.2	CG30460-RC, transcript variant C (CG30460), mRNA
0	0	2	11	22	NM_206149.1	CG30460-RA, transcript variant A (CG30460), mRNA
0	0	2	11	20	NM_206150.1	CG30460-RB, transcript variant B (CG30460), mRNA
0	0	2	5	4	NM_141907.2	CG10014-RA (CG10014), mRNA
0	0	2	4	14	NM_079939.2	CG5580-RA, transcript variant A (sbb), mRNA
0	0	2	4	9	NM_206161.1	CG5580-RB, transcript variant B (sbb), mRNA
0	0	2	4	9	NM_206162.1	CG5580-RC, transcript variant C (sbb), mRNA
0	0	2	4	5	NM_142957.1	CG13605-RA (CG13605), mRNA
0	0	2	4	3	NM_144357.2	CG13475-RA (HGTX), mRNA
0	0	2	3	13	NM_166446.1	CG9696-RD, transcript variant D (dom), mRNA
0	0	2	3	13	NM_080094.2	CG9696-RA, transcript variant A (dom), mRNA
0	0	2	3	3	NM_001014522.1	CG4696-RB, transcript variant B (Mp20), mRNA
0	0	2	3	3	NM_057295.3	CG4696-RA, transcript variant A (Mp20), mRNA
0	0	2	2	23	NM_078717.2	CG3696-RA, transcript variant A (kis), mRNA
0	0	2	2	7	NM_137564.2	CG7417-RA (Tab2), mRNA
0	0	2	2	7	NM_164716.1	CG31632-RA (CG31632), mRNA
0	0	2	2	5	NM_206509.1	CG8053-RB, transcript variant B (eIF-1A), mRNA
0	0	2	2	5	NM_079989.2	CG8053-RA, transcript variant A (eIF-1A), mRNA
0	0	1	6	5	NM_140901.2	CG7762-RA (Rpn1), mRNA
0	0	1	4	15	NM_132157.1	CG9650-RB, transcript variant B (CG9650), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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