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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8601R-7

Symbol

mus312

Full Name

mutagen-sensitive 312

CG No

CG8601

Old CG No

CG8601

Synonyms

CG8601, mus(3)312, mus312

Accession No (Link to NCBI)

NM_139847.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAGCCAACTTCAAAAACCTGC

Primer Seq. 3'

aatctagaggtaccCTCAGTGGACTTCTCTTCATC

Predicted Fragment Size

IR fragment full Seq

0001 NAGCCAACTT CAAAAACCTG CAGCCTCGTT TGACGCGAAG CACCAAAGCA GCCGCAGATA 
0061 CATTATCGTT ATCCAACTTC TTTGCCACAC CCGAAAATGC TGAAGAAGTT CCGTCATGTC 
0121 TCGCCGACAT TCCTGTTCAG CCCGATCCTC CTGCGCCAAA AATCAAACCA ATACGCACCA 
0181 GCAATGTGTT CGAAAAANCC TGGGCCCAAG CCCAAAAAGG CACCCAAGCT CAAGGCCAGC 
0241 ACTGTGCCAA CGTCGAGTGG GGCGCGTCGT GGACGCGGAA AGAGCAAACA ACAGCCCAGT 
0301 ATCAGCAACT TTCTACGCAA CGAGCAGATC TTCGCGGAGG TTACCGCCCA GCATTGCATG 
0361 GCGGACAACT TTAGTCCGGA TGACATTGAA ATGGCGCTGG CGCTGTCCAA ATCGGAGAGT 
0421 GAGAAGCATG GACGCCTGCG tCTGCACGAC GATGACGATG CGGTGGTTGA CCTACTGGAT 
0481 GATGAAGAGA AGTCCACTGA G

in silico PCR Fragment

0001 GAGCCAACTT CAAAAACCTG CAGCCTCGTT TGACGCGAAG CACCAAAGCA GCCGCAGATA 
0061 CATTATCGTT ATCCAACTTC TTTGCCACAC CCGAAAATGC TGAAGAAGTT CCGTCATGTC 
0121 TCGCCGACAT TCCTGTTCAG CCCGATCCTC CTGCGCCAAA AATCAAACCA ATACGCACCA 
0181 GCAATGTGTT CGAAAAA-CC TGGGCCCAAG CCCAAAAAGG CACCCAAGCT CAAGGCCAGC 
0241 ACTGTGTCAA CGTCGAGTGG GGCGCGTCGT GGACGCGGAA AGAGCAAACA ACAGCCCAGT 
0301 ATCAGCAACT TTCTACGCAA CGAGCAGATC TTCGCGGAGG TTACCGCCCA GCATTGCATG 
0361 GCGGACAACT TTAGTGCGGA TGACATTGAA ATGGCGCTGG CGCTGTCCAA ATCGGAGAGT 
0421 GAGAAGCATG GACGCCTTCG TCTGCATGAC GATGACGATG CGGTGGTTGA CCTACTGGAT 
0481 GATGAAGAGA AGTCCACTGA G

Assemble Data

<PRE>

8601R-7.IR_full        1   AGCCAACTTCAAAAACCTGCAGCCTCGTTTGACGCGAAGCACCAAAGCAGCCGCAGATA 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8601R-7.in silico      1   AGCCAACTTCAAAAACCTGCAGCCTCGTTTGACGCGAAGCACCAAAGCAGCCGCAGATA 59


8601R-7.IR_full       61  CATTATCGTTATCCAACTTCTTTGCCACACCCGAAAATGCTGAAGAAGTTCCGTCATGTC 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8601R-7.in silico     61  CATTATCGTTATCCAACTTCTTTGCCACACCCGAAAATGCTGAAGAAGTTCCGTCATGTC 119


8601R-7.IR_full       121 TCGCCGACATTCCTGTTCAGCCCGATCCTCCTGCGCCAAAAATCAAACCAATACGCACCA 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8601R-7.in silico     121 TCGCCGACATTCCTGTTCAGCCCGATCCTCCTGCGCCAAAAATCAAACCAATACGCACCA 179


8601R-7.IR_full       181 GCAATGTGTTCGAAAAA<span class="snp"><tt>N</tt></span>CCTGGGCCCAAGCCCAAAAAGGCACCCAAGCTCAAGGCCAGC 239
                          |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||
8601R-7.in silico     181 GCAATGTGTTCGAAAAA<span class="snp"><tt>-</tt></span>CCTGGGCCCAAGCCCAAAAAGGCACCCAAGCTCAAGGCCAGC 239


8601R-7.IR_full       241 ACTGTG<span class="snp"><tt>C</tt></span>CAACGTCGAGTGGGGCGCGTCGTGGACGCGGAAAGAGCAAACAACAGCCCAGT 299
                          ||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
8601R-7.in silico     241 ACTGTG<span class="snp"><tt>T</tt></span>CAACGTCGAGTGGGGCGCGTCGTGGACGCGGAAAGAGCAAACAACAGCCCAGT 299


8601R-7.IR_full       301 ATCAGCAACTTTCTACGCAACGAGCAGATCTTCGCGGAGGTTACCGCCCAGCATTGCATG 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8601R-7.in silico     301 ATCAGCAACTTTCTACGCAACGAGCAGATCTTCGCGGAGGTTACCGCCCAGCATTGCATG 359


8601R-7.IR_full       361 GCGGACAACTTTAGT<span class="snp"><tt>C</tt></span>CGGATGACATTGAAATGGCGCTGGCGCTGTCCAAATCGGAGAGT 419
                          |||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||
8601R-7.in silico     361 GCGGACAACTTTAGT<span class="snp"><tt>G</tt></span>CGGATGACATTGAAATGGCGCTGGCGCTGTCCAAATCGGAGAGT 419


8601R-7.IR_full       421 GAGAAGCATGGACGCCT<span class="snp"><tt>G</tt></span>CGTCTGCA<span class="snp"><tt>C</tt></span>GACGATGACGATGCGGTGGTTGACCTACTGGAT 479
                          |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||
8601R-7.in silico     421 GAGAAGCATGGACGCCT<span class="snp"><tt>T</tt></span>CGTCTGCA<span class="snp"><tt>T</tt></span>GACGATGACGATGCGGTGGTTGACCTACTGGAT 479


8601R-7.IR_full       481 GATGAAGAGAAGTCCACTGAG 500
                          |||||||||||||||||||||
8601R-7.in silico     481 GATGAAGAGAAGTCCACTGAG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_001043124.1	CG8601-RC, transcript variant C (mus312), mRNA
100	482	0	0	3	NM_139847.2	CG8601-RA, transcript variant A (mus312), mRNA
100	482	0	0	3	NM_168200.1	CG8601-RB, transcript variant B (mus312), mRNA
0	0	2	3	4	NM_141009.2	CG32432-RA (CG32432), mRNA
0	0	2	3	3	NM_176132.2	CG12052-RR, transcript variant R (lola), mRNA
0	0	2	3	3	NM_080027.4	CG12052-RG, transcript variant G (lola), mRNA
0	0	1	4	8	NM_143145.1	CG5111-RA (CG5111), mRNA
0	0	1	4	2	NM_136701.1	CG12129-RA (CG12129), mRNA
0	0	1	2	9	NM_080306.2	CG3443-RB (pcx), mRNA
0	0	1	2	3	NM_137128.2	CG12864-RA, transcript variant A (Su(var)2-HP2), mRNA
0	0	1	2	3	NM_168306.1	CG5651-RB, transcript variant B (CG5651), mRNA
0	0	1	2	3	NM_140015.2	CG5651-RA, transcript variant A (CG5651), mRNA
0	0	0	7	2	NM_135512.2	CG4901-RA (CG4901), mRNA
0	0	0	5	6	NM_057656.2	CG12345-RA, transcript variant A (Cha), mRNA
0	0	0	5	6	NM_206517.1	CG12345-RB, transcript variant B (Cha), mRNA
0	0	0	4	21	NM_166125.2	CG18255-RA, transcript variant A (Strn-Mlck), mRNA
0	0	0	4	9	NM_057523.3	CG18412-RA (ph-p), mRNA
0	0	0	4	9	NM_080055.2	CG3895-RA (ph-d), mRNA
0	0	0	4	8	NM_167685.1	CG12701-RB, transcript variant B (CG12701), mRNA
0	0	0	4	8	NM_134512.4	CG12701-RA, transcript variant A (CG12701), mRNA
0	0	0	4	7	NM_141876.1	CG12213-RB, transcript variant B (CG12213), mRNA
0	0	0	4	7	NM_169442.1	CG12213-RA, transcript variant A (CG12213), mRNA
0	0	0	4	6	NM_143149.1	CG5071-RB, transcript variant B (CG5071), mRNA
0	0	0	4	6	NM_170232.1	CG5071-RA, transcript variant A (CG5071), mRNA
0	0	0	4	5	NM_166316.1	CG12758-RC, transcript variant C (sano), mRNA
0	0	0	4	5	NM_079064.2	CG12758-RD, transcript variant D (sano), mRNA
0	0	0	4	5	NM_135747.3	CG6214-RB, transcript variant B (MRP), mRNA
0	0	0	4	5	NM_205969.1	CG6214-RK, transcript variant K (MRP), mRNA
0	0	0	4	5	NM_205971.1	CG6214-RI, transcript variant I (MRP), mRNA
0	0	0	4	5	NM_205981.1	CG6214-RN, transcript variant N (MRP), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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