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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8600R-3 
 Symbol CG8600  Full Name CG8600 
 CG No CG8600  Old CG No CG8600 
 Synonyms CG8600 
 Accession No (Link to NCBI) NM_139848.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGTCCACAATCGAAGAGGAAC 
 Primer Seq. 3' 
aatctagaggtaccCGCTTCTGCAACTCCACGTAT 
 Predicted Fragment Size 
472 
 IR fragment full Seq 
0001 TGTCCACAAT CGAAGAGGAA CGCAAGGCGT ACGAAAAGAA TCCCTACTTC ACCGGCCACA 
0061 TCTACGGCAA CTTCTCGCCG TTTTATGTGA CCATTGCCAT CTGCACGGTT GTCCTGGGCA 
0121 CGATCATAAT ACTGAACATT ATCTTGGGCT GCTGCTCCAA GCATCGCAAG TACTGGCAGG 
0181 ACAGGCACAC GGGCAATCGC TGGTTGGTTT CCATTTGGTC TGCCACGCCC CACAATCAGC 
0241 CGCCATTGGA TTTCACTGAG CTGAAGGACG CCTCATACTT TCAGCGCTTC CATCCCACAA 
0301 CCCATCAGCA AGTGTTCCCC GACGACGTTG TCATCGGCGT TGACGACNTT GGAGCCCGTG 
0361 CACTCGGCGC ACCATCATCA TCACCAGCAG CAGCACCAGC GTCCACCACG TCCAGAGGGT 
0421 CGCACTTTGC ACCAGCAgCG CCAGCGCGAG GAATACGTGG AGTTGCAGAN GCG 
 in silico PCR Fragment 
0001 TGTCCACAAT CGAAGAGGAA CGCAAGGCGT ACGAAAAGAA TCCCTACTTC ACCGGCCACA 
0061 TCTACGGCAA CTTCTCGCCG TTTTATGTGA CCATTGCCAT CTGCACGGTT GTCCTGGGCA 
0121 CGATCATAAT ACTGAACATT ATCTTGGGCT GCTGCTCCAA GCATCGCAAG TACTGGCAGG 
0181 ACAGGCACAC GGGCAATCGC TGGTTGGTTT CCATTTGGTC TGCCACGCCC CACAATCAGC 
0241 CGCCATTGGA TTTCACTGAG CTGAAGGACG CCTCATACTT TCAGCGCTTC CATCCCACAA 
0301 CCCATCAGCA AGTGTTCCCC GACGACGTTG TCATCGGCGT TGACGAC-TT GGAGCCCGTG 
0361 CACTCGGCGC ACCATCATCA TCACCAGCAG CAGCACCAGC GTCCACCACG TCCAGAGGGT 
0421 CGCACTTTGC ACCAGCAGCG CCAGCGCGAG GAATACGTGG AGTTGCAGAA GCG 
 Assemble Data 
<PRE>

8600R-3.IR_full       1   TGTCCACAATCGAAGAGGAACGCAAGGCGTACGAAAAGAATCCCTACTTCACCGGCCACA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-3.in silico     1   TGTCCACAATCGAAGAGGAACGCAAGGCGTACGAAAAGAATCCCTACTTCACCGGCCACA 60


8600R-3.IR_full       61  TCTACGGCAACTTCTCGCCGTTTTATGTGACCATTGCCATCTGCACGGTTGTCCTGGGCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-3.in silico     61  TCTACGGCAACTTCTCGCCGTTTTATGTGACCATTGCCATCTGCACGGTTGTCCTGGGCA 120


8600R-3.IR_full       121 CGATCATAATACTGAACATTATCTTGGGCTGCTGCTCCAAGCATCGCAAGTACTGGCAGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-3.in silico     121 CGATCATAATACTGAACATTATCTTGGGCTGCTGCTCCAAGCATCGCAAGTACTGGCAGG 180


8600R-3.IR_full       181 ACAGGCACACGGGCAATCGCTGGTTGGTTTCCATTTGGTCTGCCACGCCCCACAATCAGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-3.in silico     181 ACAGGCACACGGGCAATCGCTGGTTGGTTTCCATTTGGTCTGCCACGCCCCACAATCAGC 240


8600R-3.IR_full       241 CGCCATTGGATTTCACTGAGCTGAAGGACGCCTCATACTTTCAGCGCTTCCATCCCACAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-3.in silico     241 CGCCATTGGATTTCACTGAGCTGAAGGACGCCTCATACTTTCAGCGCTTCCATCCCACAA 300


8600R-3.IR_full       301 CCCATCAGCAAGTGTTCCCCGACGACGTTGTCATCGGCGTTGACGAC<span class="snp"><tt>N</tt></span>TTGGAGCCCGTG 360
                          |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
8600R-3.in silico     301 CCCATCAGCAAGTGTTCCCCGACGACGTTGTCATCGGCGTTGACGAC<span class="snp"><tt>-</tt></span>TTGGAGCCCGTG 360


8600R-3.IR_full       361 CACTCGGCGCACCATCATCATCACCAGCAGCAGCACCAGCGTCCACCACGTCCAGAGGGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-3.in silico     361 CACTCGGCGCACCATCATCATCACCAGCAGCAGCACCAGCGTCCACCACGTCCAGAGGGT 420


8600R-3.IR_full       421 CGCACTTTGCACCAGCAGCGCCAGCGCGAGGAATACGTGGAGTTGCAGA<span class="snp"><tt>N</tt></span>GCG 473
                          |||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||
8600R-3.in silico     421 CGCACTTTGCACCAGCAGCGCCAGCGCGAGGAATACGTGGAGTTGCAGA<span class="snp"><tt>A</tt></span>GCG 473

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   454  0  4  14  NM_139848.2  CG8600-RA (CG8600), mRNA 
1.1   5  12  23  77  NM_139420.1  CG12361-RA (CG12361), mRNA 
1.1   5  8  16  25  NM_078513.2  CG4626-RA, transcript variant A (fz4), mRNA 
1.1   5  8  16  25  NM_167119.2  CG4626-RB, transcript variant B (fz4), mRNA 
0.44   2  12  21  71  NM_078662.2  CG5488-RA (B-H2), mRNA 
0.44   2  10  13  31  NM_079838.2  CG5099-RA, transcript variant A (msi), mRNA 
0.44   2  10  13  31  NM_170229.2  CG5099-RB, transcript variant B (msi), mRNA 
0.44   2  8  14  51  NM_168301.1  CG5735-RD, transcript variant D (orb2), mRNA 
0.44   2  8  14  51  NM_140009.1  CG5735-RB, transcript variant B (orb2), mRNA 
0.44   2  8  14  51  NM_168300.1  CG5735-RC, transcript variant C (orb2), mRNA 
0.44   2  8  14  45  NM_168302.1  CG5735-RA, transcript variant A (orb2), mRNA 
0.44   2  8  9  21  NM_170659.1  CG7835-RA (CG7835), mRNA 
0.44   2  7  25  136  NM_079507.2  CG2530-RA (corto), mRNA 
0.44   2  6  12  47  NM_169223.2  CG31369-RA (PQBP-1), mRNA 
0.44   2  2  5  4  NM_001014648.1  CG5695-RE, transcript variant E (jar), mRNA 
0.44   2  2  5  4  NM_001014647.1  CG5695-RF, transcript variant F (jar), mRNA 
0.44   2  2  5  4  NM_170138.1  CG5695-RB, transcript variant B (jar), mRNA 
0.44   2  2  5  4  NM_001014650.1  CG5695-RC, transcript variant C (jar), mRNA 
0.44   2  2  5  4  NM_079754.2  CG5695-RA, transcript variant A (jar), mRNA 
0.44   2  2  5  3  NM_001014649.1  CG5695-RD, transcript variant D (jar), mRNA 
0.22   1  6  20  76  NM_164960.1  CG32830-RA (CG32830), mRNA 
0.22   1  5  10  36  NM_176504.1  CG33207-RB, transcript variant B (pxb), mRNA 
0.22   1  4  11  45  NM_057976.2  CG1897-RA (Dr), mRNA 
0.22   1  4  7  42  NM_136982.2  CG3850-RA (sug), mRNA 
0.22   1  3  14  53  NM_164858.1  CG4405-RB, transcript variant B (jp), mRNA 
0.22   1  3  14  53  NM_078801.4  CG4405-RA, transcript variant A (jp), mRNA 
0.22   1  2  7  3  NM_164692.2  CG31638-RA (CG31638), mRNA 
0.22   1  2  4  8  NM_170236.1  CG31096-RA (Lgr3), mRNA 
0   0  10  30  61  NM_130728.1  CG15240-RA (CG15240), mRNA 
0   0  9  22  55  NM_135729.1  CG15489-RA (CG15489), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
++ 
DB
++ 
DC
++ 
DM
++ 
VM
+ 
VB
 
Caspase-3 signal
N
++ 
DB
++ 
DC
++ 
DM
++ 
VM
+ 
VB
 
Dcompartment   Penetrance
+     
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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