NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8600R-1

Symbol

CG8600

Full Name

CG8600

CG No

CG8600

Old CG No

CG8600

Synonyms

CG8600

Accession No (Link to NCBI)

NM_139848.2

Inserted Chr.

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGTCCACAATCGAAGAGGAAC

Primer Seq. 3'

aatctagaggtaccCGCTTCTGCAACTCCACGTAT

Predicted Fragment Size

IR fragment full Seq

0001 TGTCCACAAT CGAAGAGGAA CGCAAGGCGT ACGAAAAGAA TCCCTACTTC ACCGGCCACA 
0061 TCTACGGCAA CTTCTCGCCG TTTTATGTGA CCATTGCCAT CTGCACGGTT GTCCTGGGCA 
0121 CGATCATAAT ACTGAACATT ATCTTGGGCT GCTGCTCCAA GCATCGCAAG TACTGGCAGG 
0181 ACAGGCACAC GGGCAATCGC TGGTTGGTTT CCATTTGGTC TGCCACGCCC CACAATCAGC 
0241 CGCCATTGGA TTTCACTGAG CTGAAGGACG CCTCATACTT TCAGCGCTTC CATCCCACAA 
0301 CCCATCAGCA AGTGTTCCCC GACGACGTTG TCATCGGCGT TGACGACNTT GGAGCCCGTG 
0361 CACTCGGCGC ACCATCATCA TCACCAGCAG CAGCACCAGC GTCCACCACG TCCAGAGGGT 
0421 CGCACTTTGC ACCAGCAgCG CCAGCGCGAG GAATACGTGG AGTTGCAGAN GCG

in silico PCR Fragment

0001 TGTCCACAAT CGAAGAGGAA CGCAAGGCGT ACGAAAAGAA TCCCTACTTC ACCGGCCACA 
0061 TCTACGGCAA CTTCTCGCCG TTTTATGTGA CCATTGCCAT CTGCACGGTT GTCCTGGGCA 
0121 CGATCATAAT ACTGAACATT ATCTTGGGCT GCTGCTCCAA GCATCGCAAG TACTGGCAGG 
0181 ACAGGCACAC GGGCAATCGC TGGTTGGTTT CCATTTGGTC TGCCACGCCC CACAATCAGC 
0241 CGCCATTGGA TTTCACTGAG CTGAAGGACG CCTCATACTT TCAGCGCTTC CATCCCACAA 
0301 CCCATCAGCA AGTGTTCCCC GACGACGTTG TCATCGGCGT TGACGAC-TT GGAGCCCGTG 
0361 CACTCGGCGC ACCATCATCA TCACCAGCAG CAGCACCAGC GTCCACCACG TCCAGAGGGT 
0421 CGCACTTTGC ACCAGCAGCG CCAGCGCGAG GAATACGTGG AGTTGCAGAA GCG

Assemble Data

<PRE>

8600R-1.IR_full       1   TGTCCACAATCGAAGAGGAACGCAAGGCGTACGAAAAGAATCCCTACTTCACCGGCCACA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-1.in silico     1   TGTCCACAATCGAAGAGGAACGCAAGGCGTACGAAAAGAATCCCTACTTCACCGGCCACA 60


8600R-1.IR_full       61  TCTACGGCAACTTCTCGCCGTTTTATGTGACCATTGCCATCTGCACGGTTGTCCTGGGCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-1.in silico     61  TCTACGGCAACTTCTCGCCGTTTTATGTGACCATTGCCATCTGCACGGTTGTCCTGGGCA 120


8600R-1.IR_full       121 CGATCATAATACTGAACATTATCTTGGGCTGCTGCTCCAAGCATCGCAAGTACTGGCAGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-1.in silico     121 CGATCATAATACTGAACATTATCTTGGGCTGCTGCTCCAAGCATCGCAAGTACTGGCAGG 180


8600R-1.IR_full       181 ACAGGCACACGGGCAATCGCTGGTTGGTTTCCATTTGGTCTGCCACGCCCCACAATCAGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-1.in silico     181 ACAGGCACACGGGCAATCGCTGGTTGGTTTCCATTTGGTCTGCCACGCCCCACAATCAGC 240


8600R-1.IR_full       241 CGCCATTGGATTTCACTGAGCTGAAGGACGCCTCATACTTTCAGCGCTTCCATCCCACAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-1.in silico     241 CGCCATTGGATTTCACTGAGCTGAAGGACGCCTCATACTTTCAGCGCTTCCATCCCACAA 300


8600R-1.IR_full       301 CCCATCAGCAAGTGTTCCCCGACGACGTTGTCATCGGCGTTGACGAC<span class="snp"><tt>N</tt></span>TTGGAGCCCGTG 360
                          |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
8600R-1.in silico     301 CCCATCAGCAAGTGTTCCCCGACGACGTTGTCATCGGCGTTGACGAC<span class="snp"><tt>-</tt></span>TTGGAGCCCGTG 360


8600R-1.IR_full       361 CACTCGGCGCACCATCATCATCACCAGCAGCAGCACCAGCGTCCACCACGTCCAGAGGGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8600R-1.in silico     361 CACTCGGCGCACCATCATCATCACCAGCAGCAGCACCAGCGTCCACCACGTCCAGAGGGT 420


8600R-1.IR_full       421 CGCACTTTGCACCAGCAGCGCCAGCGCGAGGAATACGTGGAGTTGCAGA<span class="snp"><tt>N</tt></span>GCG 473
                          |||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||
8600R-1.in silico     421 CGCACTTTGCACCAGCAGCGCCAGCGCGAGGAATACGTGGAGTTGCAGA<span class="snp"><tt>A</tt></span>GCG 473

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	454	0	4	14	NM_139848.2	CG8600-RA (CG8600), mRNA
1.1	5	12	23	77	NM_139420.1	CG12361-RA (CG12361), mRNA
1.1	5	8	16	25	NM_078513.2	CG4626-RA, transcript variant A (fz4), mRNA
1.1	5	8	16	25	NM_167119.2	CG4626-RB, transcript variant B (fz4), mRNA
0.44	2	12	21	71	NM_078662.2	CG5488-RA (B-H2), mRNA
0.44	2	10	13	31	NM_079838.2	CG5099-RA, transcript variant A (msi), mRNA
0.44	2	10	13	31	NM_170229.2	CG5099-RB, transcript variant B (msi), mRNA
0.44	2	8	14	51	NM_168301.1	CG5735-RD, transcript variant D (orb2), mRNA
0.44	2	8	14	51	NM_140009.1	CG5735-RB, transcript variant B (orb2), mRNA
0.44	2	8	14	51	NM_168300.1	CG5735-RC, transcript variant C (orb2), mRNA
0.44	2	8	14	45	NM_168302.1	CG5735-RA, transcript variant A (orb2), mRNA
0.44	2	8	9	21	NM_170659.1	CG7835-RA (CG7835), mRNA
0.44	2	7	25	136	NM_079507.2	CG2530-RA (corto), mRNA
0.44	2	6	12	47	NM_169223.2	CG31369-RA (PQBP-1), mRNA
0.44	2	2	5	4	NM_001014648.1	CG5695-RE, transcript variant E (jar), mRNA
0.44	2	2	5	4	NM_001014647.1	CG5695-RF, transcript variant F (jar), mRNA
0.44	2	2	5	4	NM_170138.1	CG5695-RB, transcript variant B (jar), mRNA
0.44	2	2	5	4	NM_001014650.1	CG5695-RC, transcript variant C (jar), mRNA
0.44	2	2	5	4	NM_079754.2	CG5695-RA, transcript variant A (jar), mRNA
0.44	2	2	5	3	NM_001014649.1	CG5695-RD, transcript variant D (jar), mRNA
0.22	1	6	20	76	NM_164960.1	CG32830-RA (CG32830), mRNA
0.22	1	5	10	36	NM_176504.1	CG33207-RB, transcript variant B (pxb), mRNA
0.22	1	4	11	45	NM_057976.2	CG1897-RA (Dr), mRNA
0.22	1	4	7	42	NM_136982.2	CG3850-RA (sug), mRNA
0.22	1	3	14	53	NM_164858.1	CG4405-RB, transcript variant B (jp), mRNA
0.22	1	3	14	53	NM_078801.4	CG4405-RA, transcript variant A (jp), mRNA
0.22	1	2	7	3	NM_164692.2	CG31638-RA (CG31638), mRNA
0.22	1	2	4	8	NM_170236.1	CG31096-RA (Lgr3), mRNA
0	0	10	30	61	NM_130728.1	CG15240-RA (CG15240), mRNA
0	0	9	22	55	NM_135729.1	CG15489-RA (CG15489), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

Back