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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8547R-2

Symbol CG8547 Full Name CG8547

CG No CG8547 Old CG No CG8547

Synonyms CG8547

Accession No (Link to NCBI) NM_137103.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACTCGAGTTCTGGCGACAACA

Primer Seq. 3'

aatctagaggtaccAATGGTGGTGCCGCTGGTCAA

Predicted Fragment Size

IR fragment full Seq

0001 ACTCGAGTTC TGGCGACAAC AATGTCTCGT ATCAGGGCAA ATCCCCCGAC CAGAACATAG 
0061 ATCAATTGGA TGCCTTGTTG GAGGATCTGA AGCAGGAACG ACAGATTACC AACAGGGAAC 
0121 GCGATCTGCT GCCCAGCAAT GGAACCACTT ATAGGACACT TGAACGCACT GATCCCTCGG 
0181 GCACCGTAAC GAGGACTACG CGAGTGGTCA AGACCAGCAN AGCACTCGGG CACCGGTGGC 
0241 CAGCAGCCAT TTATTGATGA TGTGGAGACC TTCAGTCCCA CCGACTACAG CACCCTGCAG 
0301 TCCACGGCCA AGTACTCCAG CTTGAAGAGG GACGAGTTCC AGACCAAGGA CAAGCCCTAT 
0361 ACTCTGGCCC ACTCCCCCAG TGGTGGACAC TACGAGAGCA AGTCGAAGAT CTACGAAAGC 
0421 AATCGCACCA TTAACAACAA TGTGGAGCTG CTGCTCTGCA ACGAGCAGCA CACATCAGAC 
0481 TTTGATCCAG CGGCACCACC ATT

in silico PCR Fragment

0001 ACTCGAGTTC TGGCGACAAC AATGTCTCGT ATCAGGGCAA ATCCCCCGAC CAGAACATAG 
0061 ATCAATTGGA TGCCTTGTTG GAGGATCTGA AGCAGGAACG ACAGATTACC AACAGGGAAC 
0121 GCGATCTGCT GCCCAGCAAT GGAACCACTT ATAGGACACT TGAACGCACT GATCCCTCGG 
0181 GCACCGTAAC GAGGACTACG CGAGTGGTCA AGACCAGCA- AGCACTCGGG CACCGGTGGC 
0241 CAGCAGCCAT TTATTGATGA TGTGGAGACC TTCAGTCCCA CCGACTACAG CACCCTGCAG 
0301 TCCACGGCCA AGTACTCCAG CTTGAAGAGG GACGAGTTCC AGACCAAGGA CAAGCCCTAT 
0361 ACTCTGGCCC ACTCCCCCAG TGGTGGACAC TACGAGAGCA AGTCGAAGAT CTACGAAAGC 
0421 AATCGCACCA TTAACAACAA TGTGGAGCTG CTGC-CTGCA ACGAGCAGCA CACATCAGAC 
0481 TTTGA-CCAG CGGCACCACC ATT

Assemble Data


8547R-2.IR_full       1   ACTCGAGTTCTGGCGACAACAATGTCTCGTATCAGGGCAAATCCCCCGACCAGAACATAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8547R-2.in silico     1   ACTCGAGTTCTGGCGACAACAATGTCTCGTATCAGGGCAAATCCCCCGACCAGAACATAG 60


8547R-2.IR_full       61  ATCAATTGGATGCCTTGTTGGAGGATCTGAAGCAGGAACGACAGATTACCAACAGGGAAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8547R-2.in silico     61  ATCAATTGGATGCCTTGTTGGAGGATCTGAAGCAGGAACGACAGATTACCAACAGGGAAC 120


8547R-2.IR_full       121 GCGATCTGCTGCCCAGCAATGGAACCACTTATAGGACACTTGAACGCACTGATCCCTCGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8547R-2.in silico     121 GCGATCTGCTGCCCAGCAATGGAACCACTTATAGGACACTTGAACGCACTGATCCCTCGG 180


8547R-2.IR_full       181 GCACCGTAACGAGGACTACGCGAGTGGTCAAGACCAGCANAGCACTCGGGCACCGGTGGC 240
                          ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
8547R-2.in silico     181 GCACCGTAACGAGGACTACGCGAGTGGTCAAGACCAGCA-AGCACTCGGGCACCGGTGGC 240


8547R-2.IR_full       241 CAGCAGCCATTTATTGATGATGTGGAGACCTTCAGTCCCACCGACTACAGCACCCTGCAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8547R-2.in silico     241 CAGCAGCCATTTATTGATGATGTGGAGACCTTCAGTCCCACCGACTACAGCACCCTGCAG 300


8547R-2.IR_full       301 TCCACGGCCAAGTACTCCAGCTTGAAGAGGGACGAGTTCCAGACCAAGGACAAGCCCTAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8547R-2.in silico     301 TCCACGGCCAAGTACTCCAGCTTGAAGAGGGACGAGTTCCAGACCAAGGACAAGCCCTAT 360


8547R-2.IR_full       361 ACTCTGGCCCACTCCCCCAGTGGTGGACACTACGAGAGCAAGTCGAAGATCTACGAAAGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8547R-2.in silico     361 ACTCTGGCCCACTCCCCCAGTGGTGGACACTACGAGAGCAAGTCGAAGATCTACGAAAGC 420


8547R-2.IR_full       421 AATCGCACCATTAACAACAATGTGGAGCTGCTGCTCTGCAACGAGCAGCACACATCAGAC 480
                          |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
8547R-2.in silico     421 AATCGCACCATTAACAACAATGTGGAGCTGCTGC-CTGCAACGAGCAGCACACATCAGAC 480


8547R-2.IR_full       481 TTTGATCCAGCGGCACCACCATT 503
                          ||||| |||||||||||||||||
8547R-2.in silico     481 TTTGA-CCAGCGGCACCACCATT 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_137103.2	CG8547-RA, transcript variant A (CG8547), mRNA
100	482	0	0	0	NM_166044.1	CG8547-RB, transcript variant B (CG8547), mRNA
0	0	5	3	7	NM_137247.1	CG8434-RA (lbk), mRNA
0	0	2	5	6	NM_169061.1	CG8434-RA (lbk), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA
0	0	2	5	6	NM_169060.1	CG8434-RA (lbk), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA
0	0	2	5	3	NM_143021.2	CG5796-RA (Ppox), mRNA
0	0	2	2	6	NM_143705.2	CG12225-RA (Spt6), mRNA
0	0	2	2	6	NM_139356.1	CG3524-RA (v(2)k05816), mRNA
0	0	2	2	5	NM_079039.2	CG8380-RA (DAT), mRNA
0	0	2	2	2	NM_080110.2	CG32356-RA, transcript variant A (ImpE1), mRNA
0	0	1	3	9	NM_144487.1	CG5518-RA (sda), mRNA
0	0	1	3	4	NM_140327.1	CG10522-RA (sti), mRNA
0	0	1	3	2	NM_133101.2	CG7101-RA (CG7101), mRNA
0	0	1	3	2	NM_137915.3	CG3219-RA (Klp59C), mRNA
0	0	1	2	6	NM_078650.2	CG9802-RA, transcript variant A (Cap), mRNA
0	0	1	2	6	NM_167525.1	CG9802-RB, transcript variant B (Cap), mRNA
0	0	1	2	4	NM_134898.1	CG17258-RA (CG17258), mRNA
0	0	1	2	3	NM_167649.1	CG32533-RA (CG32533), mRNA
0	0	1	2	3	NM_132858.2	CG9056-RA (CG9056), mRNA
0	0	1	2	2	NM_079262.2	CG5008-RA (GNBP3), mRNA
0	0	0	6	7	NM_142122.2	CG7832-RA (CG7832), mRNA
0	0	0	5	5	NM_143372.1	CG9995-RA (htt), mRNA
0	0	0	4	19	NM_166019.1	CG18076-RH, transcript variant H (shot), mRNA
0	0	0	4	12	NM_078670.2	CG7092-RA (Dhc16F), mRNA
0	0	0	4	5	NM_079866.2	CG1744-RA (chp), mRNA
0	0	0	4	4	NM_080378.2	CG1744-RA (chp), mRNA,2-N-acetylglucosaminyltransferase I CG13431-RA (Mgat1), mRNA
0	0	0	4	2	NM_136527.1	CG14755-RA (CG14755), mRNA
0	0	0	4	2	NM_080314.2	CG8590-RA (Klp3A), mRNA
0	0	0	3	14	NM_166018.1	CG18076-RC, transcript variant C (shot), mRNA
0	0	0	3	13	NM_078653.1	CG18572-RA, transcript variant A (r), mRNA

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