NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8539R-1

Symbol

CG8539

Full Name

CG8539

CG No

CG8539

Old CG No

CG8539

Synonyms

CG8539

Accession No (Link to NCBI)

NM_139873.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGAAGAGGCCTTATGCTCCAA

Primer Seq. 3'

aatctagaggtaccGAACTACCAGCGTAGTTCTCA

Predicted Fragment Size

IR fragment full Seq

0001 CGAAGAGGCC TTATGCTCCA ATTGGACAAC TACCTTAGCT ACGATGGAAT AATGCAGTAT 
0061 CTGGATGAGC TGGCTCTGTC CCACAGTAAC CGGGTGACCC TAAAGGATGT GGCTCGGACC 
0121 TACGAGAACA GAGCCCTGAA AATGGCGATA ATAACCAACG GAGATGGACG TCCCGGAAAG 
0181 AGAGTGATAT TCTTGGATGC TGCCCTCCAT TCGCGTGAGT GGATGACTCC CGCCGCCGCC 
0241 CTGCTCACCA TCCATAAACT GGTGGTGGAG TTTGCGGAGA ATTCCGACCT TTTGACTGAT 
0301 TACGATTGGC ACATTATGCC ACTGGCAAAT CCAGATGGCT ACGAGTATTC ACGGAACACA 
0361 GAGCGATACT GGAGAAACAC GAGGACACCG AATGGCGGCA ACTGTTTTGG CACCAATCTC 
0421 AACAGGAATT TTGCCGTTGA CTGGAATGTG GGNTTTTCCA GAACTAAAGG ATCCCTGCGA 
0481 TGAGAACTAC GCTGGTAGTT C

in silico PCR Fragment

0001 CGAAGAGGCC TTATGCTCCA ATTGGACAAC TACCTTAGCT ACGATGGAAT AATGCAGTAT 
0061 CTGGATGAGC TGGCTCTGTC CCACAGTAAC CGGGTGACCC TAAAGGATGT GGCTCGGACC 
0121 TACGAGAACA GAGCCCTGAA AATGGCGATA ATAACCAACG GAGATGGACG TCCCGGAAAG 
0181 AGAGTGATAT TCTTGGATGC TGCCCTCCAT TCGCGTGAGT GGATGACTCC CGCCGCCGCC 
0241 CTGCTCACCA TCCATAAACT GGTGGTGGAG TTTGCGGAGA ATTCCGACCT TTTGACTGAT 
0301 TACGATTGGC ACATTATGCC ACTGGCAAAT CCAGATGGCT ACGAGTATTC ACGGAACACA 
0361 GAGCGATACT GGAGAAACAC GAGGACACCG AATGGCGGCA ACTGTTTTGG CACCAATCTC 
0421 AACAGGAATT TTGCCGTTGA CTGGAATGTG GG-TTTTCCA GAACTAAAGG ATCCCTGCGA 
0481 TGAGAACTAC GCTGGTAGTT C

Assemble Data

<PRE>

8539R-1.IR_full       1   CGAAGAGGCCTTATGCTCCAATTGGACAACTACCTTAGCTACGATGGAATAATGCAGTAT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8539R-1.in silico     1   CGAAGAGGCCTTATGCTCCAATTGGACAACTACCTTAGCTACGATGGAATAATGCAGTAT 60


8539R-1.IR_full       61  CTGGATGAGCTGGCTCTGTCCCACAGTAACCGGGTGACCCTAAAGGATGTGGCTCGGACC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8539R-1.in silico     61  CTGGATGAGCTGGCTCTGTCCCACAGTAACCGGGTGACCCTAAAGGATGTGGCTCGGACC 120


8539R-1.IR_full       121 TACGAGAACAGAGCCCTGAAAATGGCGATAATAACCAACGGAGATGGACGTCCCGGAAAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8539R-1.in silico     121 TACGAGAACAGAGCCCTGAAAATGGCGATAATAACCAACGGAGATGGACGTCCCGGAAAG 180


8539R-1.IR_full       181 AGAGTGATATTCTTGGATGCTGCCCTCCATTCGCGTGAGTGGATGACTCCCGCCGCCGCC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8539R-1.in silico     181 AGAGTGATATTCTTGGATGCTGCCCTCCATTCGCGTGAGTGGATGACTCCCGCCGCCGCC 240


8539R-1.IR_full       241 CTGCTCACCATCCATAAACTGGTGGTGGAGTTTGCGGAGAATTCCGACCTTTTGACTGAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8539R-1.in silico     241 CTGCTCACCATCCATAAACTGGTGGTGGAGTTTGCGGAGAATTCCGACCTTTTGACTGAT 300


8539R-1.IR_full       301 TACGATTGGCACATTATGCCACTGGCAAATCCAGATGGCTACGAGTATTCACGGAACACA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8539R-1.in silico     301 TACGATTGGCACATTATGCCACTGGCAAATCCAGATGGCTACGAGTATTCACGGAACACA 360


8539R-1.IR_full       361 GAGCGATACTGGAGAAACACGAGGACACCGAATGGCGGCAACTGTTTTGGCACCAATCTC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8539R-1.in silico     361 GAGCGATACTGGAGAAACACGAGGACACCGAATGGCGGCAACTGTTTTGGCACCAATCTC 420


8539R-1.IR_full       421 AACAGGAATTTTGCCGTTGACTGGAATGTGGG<span class="snp"><tt>N</tt></span>TTTTCCAGAACTAAAGGATCCCTGCGA 480
                          ||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||
8539R-1.in silico     421 AACAGGAATTTTGCCGTTGACTGGAATGTGGG<span class="snp"><tt>-</tt></span>TTTTCCAGAACTAAAGGATCCCTGCGA 480


8539R-1.IR_full       481 TGAGAACTACGCTGGTAGTTC 501
                          |||||||||||||||||||||
8539R-1.in silico     481 TGAGAACTACGCTGGTAGTTC 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139873.2	CG8539-RA (CG8539), mRNA
0.41	2	11	4	9	NM_132032.1	CG15769-RA (CG15769), mRNA
0	0	2	4	5	NM_132031.1	CG3097-RA (CG3097), mRNA
0	0	2	2	2	NM_142429.2	CG7957-RA (MED17), mRNA
0	0	1	3	3	NM_166568.1	CG3499-RB (CG3499), mRNA
0	0	1	3	2	NM_139401.1	CG13933-RA (CG13933), mRNA
0	0	1	2	4	NM_138067.1	CG15874-RA (CG15874), mRNA
0	0	1	2	2	NM_164960.1	CG32830-RA (CG32830), mRNA
0	0	0	9	14	NM_132034.1	CG3108-RA (CG3108), mRNA
0	0	0	5	4	NM_143009.1	CG6607-RA (CG6607), mRNA
0	0	0	5	4	NM_001043246.1	CG4154-RD, transcript variant D (Gyc88E), mRNA
0	0	0	5	4	NM_142167.2	CG4154-RC, transcript variant C (Gyc88E), mRNA
0	0	0	5	4	NM_169616.2	CG4154-RA, transcript variant A (Gyc88E), mRNA
0	0	0	4	8	NM_176337.1	CG33158-RB (CG33158), mRNA
0	0	0	4	6	NM_169609.1	CG31304-RA (CG31304), mRNA
0	0	0	4	4	NM_141542.1	CG11718-RA (CG11718), mRNA
0	0	0	4	3	NM_143756.2	CG1548-RA (cathD), mRNA
0	0	0	4	3	NM_143523.1	CG15531-RA (CG15531), mRNA
0	0	0	4	3	NM_142294.2	CG14886-RA (Gyc-89Db), mRNA
0	0	0	4	3	NM_080119.3	CG6143-RB, transcript variant B (Pep), mRNA
0	0	0	4	3	NM_132722.2	CG1434-RA (CG1434), mRNA
0	0	0	4	3	NM_206396.1	CG6143-RC, transcript variant C (Pep), mRNA
0	0	0	4	2	NM_169351.1	CG4067-RC, transcript variant C (pug), mRNA
0	0	0	4	2	NM_169350.1	CG4067-RB, transcript variant B (pug), mRNA
0	0	0	4	2	NM_001014614.1	CG4067-RD, transcript variant D (pug), mRNA
0	0	0	4	2	NM_164753.1	CG6441-RB, transcript variant B (CG6441), mRNA
0	0	0	4	2	NM_057906.3	CG4067-RA, transcript variant A (pug), mRNA
0	0	0	4	2	NM_135280.2	CG6441-RA, transcript variant A (CG6441), mRNA
0	0	0	3	16	NM_166019.1	CG18076-RH, transcript variant H (shot), mRNA
0	0	0	3	12	NM_166017.1	CG18076-RE, transcript variant E (shot), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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