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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8501R-2

Symbol CG8501 Full Name CG8501

CG No CG8501 Old CG No CG8501

Synonyms CG8501

Accession No (Link to NCBI) NM_136927.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGGCAGCGGATCTGAGCTT

Primer Seq. 3'

aatctagaggtaccGACGGGAAGAAGGATTTTGCC

Predicted Fragment Size

IR fragment full Seq

0001 ATGGGCAGCG GATCTGAGCT TCCACTACGG ATCTGTATGG TCTGGCTGGT CGCGGGTCTG 
0061 GCGATTGGCG ANGGCACTCC GGTGCTACGA GTGTGTCGAT CAGGAGACGT CGTGCGGATC 
0121 TGCTGATAAT TCGCCGGGAA GAGTGCGAGA ATGCCCGAAC TCAACCATGT GCTCGACCAC 
0181 CATGCTGACC ACAATGGTGA ATGGAAATGA GTGGATCAGG GTGAGGCGAG GCTGTGCCAA 
0241 GCAGGTGGAT CATTACTTTG ACTATATTGG AAAGCATTGG GAGCAGAAGT ATCGACTGAT 
0301 GGACTTACCG GAGGGCTGCA AGAAGGAGAA TGGAAGGATG AATTGCAACT GTCGCGGAGA 
0361 ACTGTGCAAT TCAGGAAGTC ATTCGACACC AAATTTCCGG TTGCCGTTCG TGCTGATCTT 
0421 ATTGGCCCTG GTCTGCGGCA AAATCCTTCT TCCCCGTC

in silico PCR Fragment

0001 ATGGGCAGCG GATCTGAGCT TCCACTACGG ATCTGTATGG TCTGGCTGGT CGCGGGTCTG 
0061 GCGATTGGCG A-GGCACTCC GGTGCTACGA GTGTGTCGAT CAGGAGACGT CGTGCGGATC 
0121 TGCTGATAAT TCGCCGGGAA GAGTGCGAGA ATGCCCGAAC TCAACCATGT GCTCGACCAC 
0181 CATGCTGACC ACAATGGTGA ATGGAAATGA GTGGATCAGG GTGAGGCGAG GCTGTGCCAA 
0241 GCAGGTGGAT CATTACTTTG ACTATATTGG AAAGCATTGG GAGCAGAAGT ATCGACTGAT 
0301 GGACTTACCG GAGGGCTGCA AGAAGGAGAA TGGAAGGATG AATTGCAACT GTCGCGGAGA 
0361 ACTTTGCAAT TCAGGAAGTC ATTCGACACC AAATTTCCGG TTGCCGTTCG TGCTGATCTT 
0421 ATTGGCCCTG GTCTGCGGCA AAATCCTTCT T-CCCGTC

Assemble Data


8501R-2.IR_full       1   ATGGGCAGCGGATCTGAGCTTCCACTACGGATCTGTATGGTCTGGCTGGTCGCGGGTCTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8501R-2.in silico     1   ATGGGCAGCGGATCTGAGCTTCCACTACGGATCTGTATGGTCTGGCTGGTCGCGGGTCTG 60


8501R-2.IR_full       61  GCGATTGGCGANGGCACTCCGGTGCTACGAGTGTGTCGATCAGGAGACGTCGTGCGGATC 120
                          ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
8501R-2.in silico     61  GCGATTGGCGA-GGCACTCCGGTGCTACGAGTGTGTCGATCAGGAGACGTCGTGCGGATC 120


8501R-2.IR_full       121 TGCTGATAATTCGCCGGGAAGAGTGCGAGAATGCCCGAACTCAACCATGTGCTCGACCAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8501R-2.in silico     121 TGCTGATAATTCGCCGGGAAGAGTGCGAGAATGCCCGAACTCAACCATGTGCTCGACCAC 180


8501R-2.IR_full       181 CATGCTGACCACAATGGTGAATGGAAATGAGTGGATCAGGGTGAGGCGAGGCTGTGCCAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8501R-2.in silico     181 CATGCTGACCACAATGGTGAATGGAAATGAGTGGATCAGGGTGAGGCGAGGCTGTGCCAA 240


8501R-2.IR_full       241 GCAGGTGGATCATTACTTTGACTATATTGGAAAGCATTGGGAGCAGAAGTATCGACTGAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8501R-2.in silico     241 GCAGGTGGATCATTACTTTGACTATATTGGAAAGCATTGGGAGCAGAAGTATCGACTGAT 300


8501R-2.IR_full       301 GGACTTACCGGAGGGCTGCAAGAAGGAGAATGGAAGGATGAATTGCAACTGTCGCGGAGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8501R-2.in silico     301 GGACTTACCGGAGGGCTGCAAGAAGGAGAATGGAAGGATGAATTGCAACTGTCGCGGAGA 360


8501R-2.IR_full       361 ACTGTGCAATTCAGGAAGTCATTCGACACCAAATTTCCGGTTGCCGTTCGTGCTGATCTT 420
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8501R-2.in silico     361 ACTTTGCAATTCAGGAAGTCATTCGACACCAAATTTCCGGTTGCCGTTCGTGCTGATCTT 420


8501R-2.IR_full       421 ATTGGCCCTGGTCTGCGGCAAAATCCTTCTTCCCCGTC 458
                          ||||||||||||||||||||||||||||||| ||||||
8501R-2.in silico     421 ATTGGCCCTGGTCTGCGGCAAAATCCTTCTT-CCCGTC 458

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	438	0	0	0	NM_136927.2	CG8501-RA (CG8501), mRNA
0	0	4	2	4	NM_169145.1	CG15186-RB, transcript variant B (CG15186), mRNA
0	0	4	2	4	NM_206436.1	CG15186-RC, transcript variant C (CG15186), mRNA
0	0	4	2	4	NM_141402.1	CG15186-RA, transcript variant A (CG15186), mRNA
0	0	4	2	2	NM_138129.2	CG16896-RA (CG16896), mRNA
0	0	1	4	3	NM_135168.3	CG9491-RA (Gef26), mRNA
0	0	1	3	8	NM_057665.3	CG15444-RA, transcript variant A (ine), mRNA
0	0	1	3	8	NM_175958.1	CG15444-RC, transcript variant C (ine), mRNA
0	0	1	3	8	NM_175959.1	CG15444-RD, transcript variant D (ine), mRNA
0	0	1	3	8	NM_057664.4	CG15444-RB, transcript variant B (ine), mRNA
0	0	1	3	5	NM_139631.1	CG15012-RB (CG15012), mRNA
0	0	1	3	2	NM_206006.2	CG10604-RB, transcript variant B (bsh), mRNA
0	0	1	2	8	NM_134507.1	CG14234-RA (CG14234), mRNA
0	0	1	2	2	NM_166134.2	CG8395-RA (Rrp42), mRNA
0	0	0	5	1	NM_168885.1	CG10523-RC, transcript variant C (park), mRNA
0	0	0	5	1	NM_168884.1	CG10523-RB, transcript variant B (park), mRNA
0	0	0	4	3	NM_080099.2	CG4062-RA, transcript variant A (Aats-val), mRNA
0	0	0	4	3	NM_141830.2	CG6790-RA (CG6790), mRNA
0	0	0	4	3	NM_165970.1	CG4062-RB, transcript variant B (Aats-val), mRNA
0	0	0	4	2	NM_140861.1	CG9451-RA (CG9451), mRNA
0	0	0	3	8	NM_001043133.1	CG32045-RD, transcript variant D (fry), mRNA
0	0	0	3	8	NM_168357.2	CG32045-RB, transcript variant B (fry), mRNA
0	0	0	3	6	NM_141935.2	CG5663-RA (Dip-C), mRNA
0	0	0	3	6	NM_001042870.1	CG34126-RB (CG34126), mRNA
0	0	0	3	6	NM_135479.3	CG4594-RA (CG4594), mRNA
0	0	0	3	6	NM_141257.1	CG12591-RA, transcript variant A (dpr16), mRNA
0	0	0	3	4	NM_167232.1	CG2890-RA, transcript variant A (PPP4R2r), mRNA
0	0	0	3	4	NM_206677.1	CG2890-RC, transcript variant C (PPP4R2r), mRNA
0	0	0	3	4	NM_080344.2	CG2890-RB, transcript variant B (PPP4R2r), mRNA
0	0	0	3	4	NM_001043144.1	CG4998-RB, transcript variant B (CG4998), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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