NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8370R-2

Symbol

CG8370

Full Name

CG8370

CG No

CG8370

Old CG No

CG8370

Synonyms

CG8370

Accession No (Link to NCBI)

NM_137229.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K. Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila. PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K. A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction. PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C. An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor. PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCGTTCTCAGACGGTGACACT

Primer Seq. 3'

aatctagaggtaccCGCTGTGATTGCCAGCTGTGC

Predicted Fragment Size

IR fragment full Seq

0001 GCGTTCTCAG ACGGTGACAC TATTCAATCA GCACTCTAAT CGAACGCTCC AGCTGAACGC 
0061 GNGTGGCCGG ACCAAGTCCG GCGTTCTACA GCAGCTTCCT GGGCACGAGA GAGGTGCCGC 
0121 CGCAAGGCAA TACAACTTTC AACGTGGTGT TCCTACCGCG CCAACTAgGC GCCATTGCCG 
0181 CCGACCTTCT CATTCACACC TCCTTCGGCC AGGCCGAGTT GGCGGTACAG GGCGAGGGCA 
0241 GTGAGTGCCC GTACCGCCTA AAGCCGTTGG TGGGCATTAA GGCGCCCATG AATGCGACAC 
0301 TCACGCCAGA GATTCACATG TACANACCCG CACGAGCGTC CGCTGCAGAT ACTTGAGATA 
0361 TACAGCAGCG GCGGCGAGTT TCAGTTAGAG TTGCCCAGCG GTGGCTCGGA GGGCCCACAA 
0421 AATCTGTGGA AAATTCCTCC ACACACTTTA AAGCCGGTCA TTAGGATATC ATTTCACGGC 
0481 cgCACAGCTG GCAATCACAG CG

in silico PCR Fragment

0001 GCGTTCTCAG ACGGTGACAC TATTCAATCA ACACTCCAAT CGAACGCTCC AGCTGAACGC 
0061 G-GTGGCCGG ACCAAGTCCG GCGTTCTACA GCAGCTTCCT GGGCACGAGA GAGGTGCCGC 
0121 CGCAAGGCAA TACAACTTTC AACGTGGTGT TCCTACCGCG CCAACTAGGC GCCATTGCCG 
0181 CCGACCTTCT CATTCACACC TCCTTCGGCC AGGCCGAGTT GGCGGTACAG GGCGAGGGCA 
0241 GTGAGTGCCC GTACCGCCTA AAGCCGTTGG TGGGCATTAA GGCGCCCATG AATGCGACAC 
0301 TCACGCCAGA GATTCACATG TACA-ACCCG CACGAGCGGC CGCTGCAGAT ACTTGAGATA 
0361 TACAGCAGCG GCGGCGAGTT TCAGTTAGAG TTGCCCAGCG GTGGCTCGGA GGGCCCACAA 
0421 AATCTGTGGA AAATTCCTCC ACACACTTTA AAGCCGGTCA TTAGGATATC ATTTCACGGC 
0481 CGCACAGCTG GCAATCACAG CG

Assemble Data

<PRE>

8370R-2.IR_full       1   GCGTTCTCAGACGGTGACACTATTCAATCA<span class="snp"><tt>G</tt></span>CACTC<span class="snp"><tt>T</tt></span>AATCGAACGCTCCAGCTGAACGC 60
                          ||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
8370R-2.in silico     1   GCGTTCTCAGACGGTGACACTATTCAATCA<span class="snp"><tt>A</tt></span>CACTC<span class="snp"><tt>C</tt></span>AATCGAACGCTCCAGCTGAACGC 60


8370R-2.IR_full       61  G<span class="snp"><tt>N</tt></span>GTGGCCGGACCAAGTCCGGCGTTCTACAGCAGCTTCCTGGGCACGAGAGAGGTGCCGC 120
                          |<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     61  G<span class="snp"><tt>-</tt></span>GTGGCCGGACCAAGTCCGGCGTTCTACAGCAGCTTCCTGGGCACGAGAGAGGTGCCGC 120


8370R-2.IR_full       121 CGCAAGGCAATACAACTTTCAACGTGGTGTTCCTACCGCGCCAACTAGGCGCCATTGCCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     121 CGCAAGGCAATACAACTTTCAACGTGGTGTTCCTACCGCGCCAACTAGGCGCCATTGCCG 180


8370R-2.IR_full       181 CCGACCTTCTCATTCACACCTCCTTCGGCCAGGCCGAGTTGGCGGTACAGGGCGAGGGCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     181 CCGACCTTCTCATTCACACCTCCTTCGGCCAGGCCGAGTTGGCGGTACAGGGCGAGGGCA 240


8370R-2.IR_full       241 GTGAGTGCCCGTACCGCCTAAAGCCGTTGGTGGGCATTAAGGCGCCCATGAATGCGACAC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     241 GTGAGTGCCCGTACCGCCTAAAGCCGTTGGTGGGCATTAAGGCGCCCATGAATGCGACAC 300


8370R-2.IR_full       301 TCACGCCAGAGATTCACATGTACA<span class="snp"><tt>N</tt></span>ACCCGCACGAGCG<span class="snp"><tt>T</tt></span>CCGCTGCAGATACTTGAGATA 360
                          ||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||
8370R-2.in silico     301 TCACGCCAGAGATTCACATGTACA<span class="snp"><tt>-</tt></span>ACCCGCACGAGCG<span class="snp"><tt>G</tt></span>CCGCTGCAGATACTTGAGATA 360


8370R-2.IR_full       361 TACAGCAGCGGCGGCGAGTTTCAGTTAGAGTTGCCCAGCGGTGGCTCGGAGGGCCCACAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     361 TACAGCAGCGGCGGCGAGTTTCAGTTAGAGTTGCCCAGCGGTGGCTCGGAGGGCCCACAA 420


8370R-2.IR_full       421 AATCTGTGGAAAATTCCTCCACACACTTTAAAGCCGGTCATTAGGATATCATTTCACGGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8370R-2.in silico     421 AATCTGTGGAAAATTCCTCCACACACTTTAAAGCCGGTCATTAGGATATCATTTCACGGC 480


8370R-2.IR_full       481 CGCACAGCTGGCAATCACAGCG 502
                          ||||||||||||||||||||||
8370R-2.in silico     481 CGCACAGCTGGCAATCACAGCG 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_137229.2	CG8370-RA (CG8370), mRNA
0	0	2	4	15	NM_175960.3	CG33196-RB (dp), mRNA
0	0	2	2	4	NM_137029.2	CG17034-RD, transcript variant D (CG17034), mRNA
0	0	2	2	4	NM_165984.1	CG17034-RA, transcript variant A (CG17034), mRNA
0	0	2	2	4	NM_170624.1	CG17034-RB, transcript variant B (CG17034), mRNA
0	0	2	2	4	NM_170625.1	CG17034-RC, transcript variant C (CG17034), mRNA
0	0	1	3	3	NM_057526.3	CG5920-RA (sop), mRNA
0	0	1	2	3	NM_132206.1	CG2253-RA (Upf2), mRNA
0	0	1	2	3	NM_142930.2	CG10198-RA (Nup98), mRNA
0	0	1	2	2	NM_166223.1	CG6556-RA (cnk), mRNA
0	0	1	2	2	NM_165204.1	CG31784-RB, transcript variant B (CG31784), mRNA
0	0	1	2	2	NM_165203.1	CG31784-RA, transcript variant A (CG31784), mRNA
0	0	0	5	5	NM_140212.1	CG7543-RA (CG7543), mRNA
0	0	0	4	5	NM_170286.1	CG31079-RA (CG31079), mRNA
0	0	0	4	4	NM_079155.2	CG9102-RA (bab2), mRNA
0	0	0	4	4	NM_169657.1	CG5166-RB, transcript variant B (Atx2), mRNA
0	0	0	4	4	NM_142209.2	CG5166-RA, transcript variant A (Atx2), mRNA
0	0	0	4	4	NM_169658.2	CG5166-RC, transcript variant C (Atx2), mRNA
0	0	0	4	4	NM_142361.2	CG5319-RA (CG5319), mRNA
0	0	0	4	3	NM_001038850.1	CG9027-RC, transcript variant C (CG9027), mRNA
0	0	0	4	3	NM_166152.1	CG8448-RA, transcript variant A (mrj), mRNA
0	0	0	4	3	NM_168898.1	CG32436-RA (CG32436), mRNA
0	0	0	4	3	NM_166154.1	CG8448-RC, transcript variant C (mrj), mRNA
0	0	0	4	3	NM_166153.1	CG8448-RB, transcript variant B (mrj), mRNA
0	0	0	4	3	NM_166155.1	CG8448-RD, transcript variant D (mrj), mRNA
0	0	0	4	2	NM_057519.3	CG17998-RA (Gprk2), mRNA
0	0	0	4	2	NM_135402.2	CG9296-RA (CG9296), mRNA
0	0	0	3	8	NM_143656.1	CG1674-RB, transcript variant B (CG1674), mRNA
0	0	0	3	7	NM_136983.2	CG17019-RA (CG17019), mRNA
0	0	0	3	7	NM_176031.1	CG33119-RA (CG33119), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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