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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8221R-1

Symbol

Amyrel

Full Name

Amyrel

CG No

CG8221

Old CG No

CG8221

Synonyms

CG8221, Amyrel

Accession No (Link to NCBI)

NM_057914.3

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Rai M, Coleman Z, Curley M, Nityanandam A, Platt A, Robles-Murguia M, Jiao J, Finkelstein D, Wang YD, Xu B, Fan Y, Demontis F.<br> Proteasome stress in skeletal muscle mounts a long-range protective response that delays retinal and brain aging.<br> Cell Metab (2021) 33(6) 1137-1154.e9 [ PubMed ID = 33773104 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAAGTTCGCTTTGACCCTGAC

Primer Seq. 3'

aatctagaggtaccGTTGCACCTGGAAGCGATCGT

Predicted Fragment Size

IR fragment full Seq

0001 CAAGTTCGCT TTGACCCTGA CGCTCTGCTT GGCGGGCAGC CTTTCGCTGG CCCAGCACAA 
0061 TCCCCATTGG TGGGGCAATC GCAACACCAT CGTCCACTTG TTCGAGTGGA AGTGGTCGGA 
0121 CATTGCCCAG GAGTGTGAGA GTTTTCTCGG ACCTCGAGGA TTCGCCGGCG TTCAAGTGAG 
0181 CCCCGTTAAT GAGAACATCT TATCGGCGGG TCGTCCGTGG TGGGAGCGAT ATCAACCCAT 
0241 CTCGTACAAG CTGACCACTC GGTCTGGTAA TGAGGAGGAA TTTGGTGACA TGGTCCGTCG 
0301 TTGCAACGAT GTGGGTGTTC GTATCTATGT GGATGTGCTG TTGAACCACA TGTCCGGAGA 
0361 TTTTGACGGT GTGGCTGTGG GCACTGCTGG AACAGAGGCG GAACCCAGTA AGAAATCCTT 
0421 CCCAGGAGTT CCATACACCG CACAGGACTT CCATCCCACC TGCGAGATTA CCGACTGGAA 
0481 CGATCGCTTC CAGGTGCAAC

in silico PCR Fragment

0001 CAAGTTCGCT TTGACCCTGA CGCTCTGCTT GGCGGGCAGC CTTTCGCTGG CCCAGCACAA 
0061 TCCCCATTGG TGGGGCAATC GCAACACCAT CGTCCACTTG TTCGAGTGGA AGTGGTCGGA 
0121 CATTGCCCAG GAGTGTGAGA GTTTTCTCGG ACCTCGAGGA TTCGCCGGCG TTCAAGTGAG 
0181 CCCCGTTAAT GAGAACATCT TATCGGCGGG TCGTCCGTGG TGGGAGCGAT ATCAACCCAT 
0241 CTCGTACAAG CTGACCACTC GGTCTGGTAA TGAGGAGGAA TTTGGTGACA TGGTCCGTCG 
0301 TTGCAACGAT GTGGGTGTTC GTATCTATGT GGATGTGCTG TTGAACCACA TGTCCGGAGA 
0361 TTTTGACGGT GTGGCTGTGG GCACTGCTGG AACAGAGGCG GAACCCAGTA AGAAATCCTT 
0421 CCCAGGAGTT CCATACACCG CACAGGACTT CCATCCCACC TGCGAGATTA CCGACTGGAA 
0481 CGATCGCTTC CAGGTGCAAC

Assemble Data

<PRE>

8221R-1.IR_full       1   CAAGTTCGCTTTGACCCTGACGCTCTGCTTGGCGGGCAGCCTTTCGCTGGCCCAGCACAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8221R-1.in silico     1   CAAGTTCGCTTTGACCCTGACGCTCTGCTTGGCGGGCAGCCTTTCGCTGGCCCAGCACAA 60


8221R-1.IR_full       61  TCCCCATTGGTGGGGCAATCGCAACACCATCGTCCACTTGTTCGAGTGGAAGTGGTCGGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8221R-1.in silico     61  TCCCCATTGGTGGGGCAATCGCAACACCATCGTCCACTTGTTCGAGTGGAAGTGGTCGGA 120


8221R-1.IR_full       121 CATTGCCCAGGAGTGTGAGAGTTTTCTCGGACCTCGAGGATTCGCCGGCGTTCAAGTGAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8221R-1.in silico     121 CATTGCCCAGGAGTGTGAGAGTTTTCTCGGACCTCGAGGATTCGCCGGCGTTCAAGTGAG 180


8221R-1.IR_full       181 CCCCGTTAATGAGAACATCTTATCGGCGGGTCGTCCGTGGTGGGAGCGATATCAACCCAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8221R-1.in silico     181 CCCCGTTAATGAGAACATCTTATCGGCGGGTCGTCCGTGGTGGGAGCGATATCAACCCAT 240


8221R-1.IR_full       241 CTCGTACAAGCTGACCACTCGGTCTGGTAATGAGGAGGAATTTGGTGACATGGTCCGTCG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8221R-1.in silico     241 CTCGTACAAGCTGACCACTCGGTCTGGTAATGAGGAGGAATTTGGTGACATGGTCCGTCG 300


8221R-1.IR_full       301 TTGCAACGATGTGGGTGTTCGTATCTATGTGGATGTGCTGTTGAACCACATGTCCGGAGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8221R-1.in silico     301 TTGCAACGATGTGGGTGTTCGTATCTATGTGGATGTGCTGTTGAACCACATGTCCGGAGA 360


8221R-1.IR_full       361 TTTTGACGGTGTGGCTGTGGGCACTGCTGGAACAGAGGCGGAACCCAGTAAGAAATCCTT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8221R-1.in silico     361 TTTTGACGGTGTGGCTGTGGGCACTGCTGGAACAGAGGCGGAACCCAGTAAGAAATCCTT 420


8221R-1.IR_full       421 CCCAGGAGTTCCATACACCGCACAGGACTTCCATCCCACCTGCGAGATTACCGACTGGAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8221R-1.in silico     421 CCCAGGAGTTCCATACACCGCACAGGACTTCCATCCCACCTGCGAGATTACCGACTGGAA 480


8221R-1.IR_full       481 CGATCGCTTCCAGGTGCAAC 500
                          ||||||||||||||||||||
8221R-1.in silico     481 CGATCGCTTCCAGGTGCAAC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_057914.3	CG8221-RA (Amyrel), mRNA
0	0	3	10	24	NM_080421.3	CG18730-RA (Amy-p), mRNA
0	0	3	10	24	NM_079044.1	CG17876-RA (Amy-d), mRNA
0	0	2	4	4	NM_169250.1	CG11980-RB, transcript variant B (CG11980), mRNA
0	0	2	4	4	NM_141600.1	CG11980-RC, transcript variant C (CG11980), mRNA
0	0	2	4	4	NM_169251.1	CG11980-RA, transcript variant A (CG11980), mRNA
0	0	2	3	3	NM_001038930.1	CG33984-RA (CG33984), mRNA
0	0	2	2	5	NM_168938.1	CG7383-RB, transcript variant B (eg), mRNA
0	0	2	2	5	NM_079482.3	CG7383-RA, transcript variant A (eg), mRNA
0	0	2	2	3	NM_143026.1	CG6879-RA (CG6879), mRNA
0	0	2	2	3	NM_141771.1	CG14694-RA (CG14694), mRNA
0	0	1	5	3	NM_135662.3	CG4970-RA, transcript variant A (CG4970), mRNA
0	0	1	5	3	NM_135663.2	CG14929-RA, transcript variant A (CG14929), mRNA
0	0	1	3	4	NM_001042877.1	CG13778-RC, transcript variant C (Mnn1), mRNA
0	0	1	3	4	NM_143610.2	CG11337-RA, transcript variant A (CG11337), mRNA
0	0	1	3	4	NM_170550.1	CG11337-RB, transcript variant B (CG11337), mRNA
0	0	1	3	4	NM_078774.3	CG13778-RA, transcript variant A (Mnn1), mRNA
0	0	1	2	6	NM_137671.1	CG15226-RA (CG15226), mRNA
0	0	1	2	6	NM_137081.2	CG30483-RA (Prosap), mRNA
0	0	1	2	5	NM_169025.1	CG2534-RB, transcript variant B (cno), mRNA
0	0	1	2	5	NM_079508.2	CG2534-RA, transcript variant A (cno), mRNA
0	0	1	2	3	NM_140410.1	CG8760-RA (CG8760), mRNA
0	0	0	22	76	NM_001015110.1	CG40323-PB.3 (CG40323), mRNA
0	0	0	6	16	NM_138247.1	CG9134-RB, transcript variant B (CG9134), mRNA
0	0	0	6	9	NM_167863.1	CG9134-RA, transcript variant A (CG9134), mRNA
0	0	0	6	9	NM_167864.1	CG9134-RC, transcript variant C (CG9134), mRNA
0	0	0	6	4	NM_132074.1	CG3585-RA (CG3585), mRNA
0	0	0	5	3	NM_143046.2	CG5814-RA (CycB3), mRNA
0	0	0	4	9	NM_001015111.1	CG40322-PA.3 (CG40322), mRNA
0	0	0	4	5	NM_137227.2	CG8315-RA (CG8315), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00500 : Starch and sucrose metabolism
dme01100 : Metabolic pathways

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