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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8189R-2

Symbol

ATPsyn-b

Full Name

ATP synthase, subunit b

CG No

CG8189

Old CG No

CG8189

Synonyms

CG8189, ATPsyn-b

Accession No (Link to NCBI)

NM_079287.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGTTCTCGAGAGCCGCTCTT

Primer Seq. 3'

aatctagaggtaccGCTGCAATGCAATGTTCTCCT

Predicted Fragment Size

IR fragment full Seq

0001 ATGTTCTCGA GAGCCGCTCT TCTAACAGCC CAGCGCTCCC TTGACCGTGG CCGCCACCCG 
0061 GTCGGCAGCC GCTGCTGCCG cTCCTGGCGG AGCCATCGAG CGCCGCCAGC GCCCCGAGCA 
0121 TCCCGGCAAG GTCCGTCTGG GATTCCTGCC CGAGGAGTGG TTCCAGTTCT TCTACAACAA 
0181 GACCGGTGTC ACCGGACCCT ACACCTTCGG CGTGGGTCTG ATCACCTATC TCTGCTCCAA 
0241 GGAGATCTAC GTCATGGAGC ACGAGTACTA CAGCGGTCTG TCGCTCGGTA TCATGGCCAT 
0301 CATTGCTGTC AAGAAGCTCG GCCCGGTCAT CGCCAAGTGG GCTGATAGCG AGATCGATAA 
0361 AATCGAATCT GAGTGGAAGG AGGGACGCGA GGCTGAGCTG AAGGTCCTCT CCGACGCCAT 
0421 CGAGGCCGAG AAGAAGGAGC AGTGGCGCGC CGACGGTGCC CTGCTGCTGA TGGAAGCCAA 
0481 GAAGGAGAAC ATTGCATTGC AGC

in silico PCR Fragment

0001 ATGTTCTCGA GAGCCGCTCT TCTAACAGCC CAGCGC-CCC TTGACCGTGG CCGCCACCCG 
0061 GTCGGCAGCC GCTGCTGCCG CTCCTGGCGG AGCCATCGAG CGCCGCCAGC GCCCCGAGCA 
0121 TCCCGGCAAG GTCCGTCTGG GATTCCTGCC CGAGGAGTGG TTCCAGTTCT TCTACAACAA 
0181 GACCGGTGTC ACCGGACCCT ACACCTTCGG CGTGGGTCTG ATCACCTATC TCTGCTCCAA 
0241 GGAGATCTAC GTCATGGAGC ACGAGTACTA CAGCGGTCTG TCGCTCGGTA TCATGGCCAT 
0301 CATTGCTGTC AAGAAGCTCG GCCCGGTCAT CGCCAAGTGG GCTGATGGCG AGATCGATAA 
0361 AATCGAATCT GAGTGGAAGG AGGGACGCGA GGCTGAGCTG AAGGTCCTCT CCGACGCCAT 
0421 CGAGGCCGAG AAGAAGGAGC AGTGGCGCGC CGACGGTGCC CTGCTGCTGA TGGAAGCCAA 
0481 GAAGGAGAAC ATTGCATTGC AGC

Assemble Data

<PRE>

8189R-2.IR_full       1   ATGTTCTCGAGAGCCGCTCTTCTAACAGCCCAGCGC<span class="snp"><tt>T</tt></span>CCCTTGACCGTGGCCGCCACCCG 60
                          ||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
8189R-2.in silico     1   ATGTTCTCGAGAGCCGCTCTTCTAACAGCCCAGCGC<span class="snp"><tt>-</tt></span>CCCTTGACCGTGGCCGCCACCCG 60


8189R-2.IR_full       61  GTCGGCAGCCGCTGCTGCCGCTCCTGGCGGAGCCATCGAGCGCCGCCAGCGCCCCGAGCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-2.in silico     61  GTCGGCAGCCGCTGCTGCCGCTCCTGGCGGAGCCATCGAGCGCCGCCAGCGCCCCGAGCA 120


8189R-2.IR_full       121 TCCCGGCAAGGTCCGTCTGGGATTCCTGCCCGAGGAGTGGTTCCAGTTCTTCTACAACAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-2.in silico     121 TCCCGGCAAGGTCCGTCTGGGATTCCTGCCCGAGGAGTGGTTCCAGTTCTTCTACAACAA 180


8189R-2.IR_full       181 GACCGGTGTCACCGGACCCTACACCTTCGGCGTGGGTCTGATCACCTATCTCTGCTCCAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-2.in silico     181 GACCGGTGTCACCGGACCCTACACCTTCGGCGTGGGTCTGATCACCTATCTCTGCTCCAA 240


8189R-2.IR_full       241 GGAGATCTACGTCATGGAGCACGAGTACTACAGCGGTCTGTCGCTCGGTATCATGGCCAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-2.in silico     241 GGAGATCTACGTCATGGAGCACGAGTACTACAGCGGTCTGTCGCTCGGTATCATGGCCAT 300


8189R-2.IR_full       301 CATTGCTGTCAAGAAGCTCGGCCCGGTCATCGCCAAGTGGGCTGAT<span class="snp"><tt>A</tt></span>GCGAGATCGATAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||
8189R-2.in silico     301 CATTGCTGTCAAGAAGCTCGGCCCGGTCATCGCCAAGTGGGCTGAT<span class="snp"><tt>G</tt></span>GCGAGATCGATAA 360


8189R-2.IR_full       361 AATCGAATCTGAGTGGAAGGAGGGACGCGAGGCTGAGCTGAAGGTCCTCTCCGACGCCAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-2.in silico     361 AATCGAATCTGAGTGGAAGGAGGGACGCGAGGCTGAGCTGAAGGTCCTCTCCGACGCCAT 420


8189R-2.IR_full       421 CGAGGCCGAGAAGAAGGAGCAGTGGCGCGCCGACGGTGCCCTGCTGCTGATGGAAGCCAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-2.in silico     421 CGAGGCCGAGAAGAAGGAGCAGTGGCGCGCCGACGGTGCCCTGCTGCTGATGGAAGCCAA 480


8189R-2.IR_full       481 GAAGGAGAACATTGCATTGCAGC 503
                          |||||||||||||||||||||||
8189R-2.in silico     481 GAAGGAGAACATTGCATTGCAGC 503

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	700	0	0	2	NM_168365.1	CG5225-RA (CG5225), mRNA, subunit b CG8189-RB, transcript variant B (ATPsyn-b), mRNA
67	469	2	4	5	NM_079287.1	CG5225-RA (CG5225), mRNA, subunit b CG8189-RB, transcript variant B (ATPsyn-b), mRNA, subunit b CG8189-RA, transcript variant A (ATPsyn-b), mRNA
0.42	3	5	4	28	NM_132172.2	CG15478-RA (CG15478), mRNA
0.42	3	2	4	9	NM_080317.2	CG2647-RA (per), mRNA
0	0	4	12	35	NM_170229.2	CG5099-RB, transcript variant B (msi), mRNA
0	0	4	11	28	NM_079838.2	CG5099-RA, transcript variant A (msi), mRNA
0	0	4	4	7	NM_001014525.1	CG33528-RD, transcript variant D (CG33528), mRNA
0	0	4	2	4	NM_080049.2	CG11614-RA (nkd), mRNA
0	0	3	5	4	NM_078921.2	CG4485-RA (Cyp9b1), mRNA
0	0	3	4	14	NM_143717.1	CG10840-RB (eIF5B), mRNA
0	0	3	3	5	NM_079877.2	CG17245-RA (plexB), mRNA
0	0	3	3	4	NR_001596.1	CR32011, mRNA
0	0	3	3	2	NR_001597.1	CR32010, mRNA
0	0	3	2	5	NM_170262.1	CG6058-RB, transcript variant B (Ald), mRNA
0	0	3	2	4	NM_170263.1	CG6058-RC, transcript variant C (Ald), mRNA
0	0	3	2	4	NM_170261.1	CG6058-RF, transcript variant F (Ald), mRNA
0	0	3	2	4	NM_170264.1	CG6058-RD, transcript variant D (Ald), mRNA
0	0	3	2	4	NM_206577.1	CG6058-RH, transcript variant H (Ald), mRNA
0	0	3	2	4	NM_170266.2	CG6058-RG, transcript variant G (Ald), mRNA
0	0	3	2	4	NM_170265.2	CG6058-RA, transcript variant A (Ald), mRNA
0	0	3	2	4	NM_079791.3	CG6058-RE, transcript variant E (Ald), mRNA
0	0	2	9	40	NM_080025.1	CG11387-RA, transcript variant A (ct), mRNA
0	0	2	5	13	NM_001014582.1	CG10488-RB, transcript variant B (eyg), mRNA
0	0	2	5	13	NM_079318.2	CG10488-RA, transcript variant A (eyg), mRNA
0	0	2	4	9	NM_167852.1	CG9097-RB, transcript variant B (bab1), mRNA
0	0	2	4	8	NM_206229.1	CG9097-RA, transcript variant A (bab1), mRNA
0	0	2	4	5	NM_134494.2	CG12235-RA (Arp11), mRNA
0	0	2	3	12	NM_057432.2	CG2762-RA (ush), mRNA
0	0	2	3	3	NM_143362.2	CG14064-RA (beat-VI), mRNA
0	0	2	3	2	NM_139936.1	CG13678-RA (CG13678), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00190 : Oxidative phosphorylation
dme01100 : Metabolic pathways

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