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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8189R-1 |
| Symbol |
ATPsyn-b |
Full Name |
ATP synthase, subunit b |
| CG No |
CG8189 |
Old CG No |
CG8189 |
| Synonyms |
CG8189, ATPsyn-b |
| Accession No (Link to NCBI) |
NM_079287.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ham SJ, Lee D, Xu WJ, Cho E, Choi S, Min S, Park S, Chung J.<br> Loss of UCHL1 rescues the defects related to Parkinson's disease by suppressing glycolysis.<br> Sci Adv (2021) 7(28)
[
PubMed ID = 34244144
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGTTCTCGAGAGCCGCTCTT |
| Primer Seq. 3' |
aatctagaggtaccGCTGCAATGCAATGTTCTCCT |
| Predicted Fragment Size |
502 |
| IR fragment full Seq |
0001 ATGTTCTCGA GAGCCGCTCT TCTAACAGCC CAGCGCTCCC TTGACCGTGG CCGCCACCCG
0061 GTCGGCAGCC GCTGCTGCCG cTCCTGGCGG AGCCATCGAG CGCCGCCAGC GCCCCGAGCA
0121 TCCCGGCAAG GTCCGTCTGG GATTCCTGCC CGAGGAGTGG TTCCAGTTCT TCTACAACAA
0181 GACCGGTGTC ACCGGACCCT ACACCTTCGG CGTGGGTCTG ATCACCTATC TCTGCTCCAA
0241 GGAGATCTAC GTCATGGAGC ACGAGTACTA CAGCGGTCTG TCGCTCGGTA TCATGGCCAT
0301 CATTGCTGTC AAGAAGCTCG GCCCGGTCAT CGCCAAGTGG GCTGATAGCG AGATCGATAA
0361 AATCGAATCT GAGTGGAAGG AGGGACGCGA GGCTGAGCTG AAGGTCCTCT CCGACGCCAT
0421 CGAGGCCGAG AAGAAGGAGC AGTGGCGCGC CGACGGTGCC CTGCTGCTGA TGGAAGCCAA
0481 GAAGGAGAAC ATTGCATTGC AGC |
| in silico PCR Fragment |
0001 ATGTTCTCGA GAGCCGCTCT TCTAACAGCC CAGCGC-CCC TTGACCGTGG CCGCCACCCG
0061 GTCGGCAGCC GCTGCTGCCG CTCCTGGCGG AGCCATCGAG CGCCGCCAGC GCCCCGAGCA
0121 TCCCGGCAAG GTCCGTCTGG GATTCCTGCC CGAGGAGTGG TTCCAGTTCT TCTACAACAA
0181 GACCGGTGTC ACCGGACCCT ACACCTTCGG CGTGGGTCTG ATCACCTATC TCTGCTCCAA
0241 GGAGATCTAC GTCATGGAGC ACGAGTACTA CAGCGGTCTG TCGCTCGGTA TCATGGCCAT
0301 CATTGCTGTC AAGAAGCTCG GCCCGGTCAT CGCCAAGTGG GCTGATGGCG AGATCGATAA
0361 AATCGAATCT GAGTGGAAGG AGGGACGCGA GGCTGAGCTG AAGGTCCTCT CCGACGCCAT
0421 CGAGGCCGAG AAGAAGGAGC AGTGGCGCGC CGACGGTGCC CTGCTGCTGA TGGAAGCCAA
0481 GAAGGAGAAC ATTGCATTGC AGC |
| Assemble Data |
<PRE>
8189R-1.IR_full 1 ATGTTCTCGAGAGCCGCTCTTCTAACAGCCCAGCGC<span class="snp"><tt>T</tt></span>CCCTTGACCGTGGCCGCCACCCG 60
||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||
8189R-1.in silico 1 ATGTTCTCGAGAGCCGCTCTTCTAACAGCCCAGCGC<span class="snp"><tt>-</tt></span>CCCTTGACCGTGGCCGCCACCCG 60
8189R-1.IR_full 61 GTCGGCAGCCGCTGCTGCCGCTCCTGGCGGAGCCATCGAGCGCCGCCAGCGCCCCGAGCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-1.in silico 61 GTCGGCAGCCGCTGCTGCCGCTCCTGGCGGAGCCATCGAGCGCCGCCAGCGCCCCGAGCA 120
8189R-1.IR_full 121 TCCCGGCAAGGTCCGTCTGGGATTCCTGCCCGAGGAGTGGTTCCAGTTCTTCTACAACAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-1.in silico 121 TCCCGGCAAGGTCCGTCTGGGATTCCTGCCCGAGGAGTGGTTCCAGTTCTTCTACAACAA 180
8189R-1.IR_full 181 GACCGGTGTCACCGGACCCTACACCTTCGGCGTGGGTCTGATCACCTATCTCTGCTCCAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-1.in silico 181 GACCGGTGTCACCGGACCCTACACCTTCGGCGTGGGTCTGATCACCTATCTCTGCTCCAA 240
8189R-1.IR_full 241 GGAGATCTACGTCATGGAGCACGAGTACTACAGCGGTCTGTCGCTCGGTATCATGGCCAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-1.in silico 241 GGAGATCTACGTCATGGAGCACGAGTACTACAGCGGTCTGTCGCTCGGTATCATGGCCAT 300
8189R-1.IR_full 301 CATTGCTGTCAAGAAGCTCGGCCCGGTCATCGCCAAGTGGGCTGAT<span class="snp"><tt>A</tt></span>GCGAGATCGATAA 360
||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||
8189R-1.in silico 301 CATTGCTGTCAAGAAGCTCGGCCCGGTCATCGCCAAGTGGGCTGAT<span class="snp"><tt>G</tt></span>GCGAGATCGATAA 360
8189R-1.IR_full 361 AATCGAATCTGAGTGGAAGGAGGGACGCGAGGCTGAGCTGAAGGTCCTCTCCGACGCCAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-1.in silico 361 AATCGAATCTGAGTGGAAGGAGGGACGCGAGGCTGAGCTGAAGGTCCTCTCCGACGCCAT 420
8189R-1.IR_full 421 CGAGGCCGAGAAGAAGGAGCAGTGGCGCGCCGACGGTGCCCTGCTGCTGATGGAAGCCAA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8189R-1.in silico 421 CGAGGCCGAGAAGAAGGAGCAGTGGCGCGCCGACGGTGCCCTGCTGCTGATGGAAGCCAA 480
8189R-1.IR_full 481 GAAGGAGAACATTGCATTGCAGC 503
|||||||||||||||||||||||
8189R-1.in silico 481 GAAGGAGAACATTGCATTGCAGC 503
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
700 |
0 |
0 |
2 |
NM_168365.1 |
CG5225-RA (CG5225), mRNA, subunit b CG8189-RB, transcript variant B (ATPsyn-b), mRNA |
| 67 |
469 |
2 |
4 |
5 |
NM_079287.1 |
CG5225-RA (CG5225), mRNA, subunit b CG8189-RB, transcript variant B (ATPsyn-b), mRNA, subunit b CG8189-RA, transcript variant A (ATPsyn-b), mRNA |
| 0.42 |
3 |
5 |
4 |
28 |
NM_132172.2 |
CG15478-RA (CG15478), mRNA |
| 0.42 |
3 |
2 |
4 |
9 |
NM_080317.2 |
CG2647-RA (per), mRNA |
| 0 |
0 |
4 |
12 |
35 |
NM_170229.2 |
CG5099-RB, transcript variant B (msi), mRNA |
| 0 |
0 |
4 |
11 |
28 |
NM_079838.2 |
CG5099-RA, transcript variant A (msi), mRNA |
| 0 |
0 |
4 |
4 |
7 |
NM_001014525.1 |
CG33528-RD, transcript variant D (CG33528), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_080049.2 |
CG11614-RA (nkd), mRNA |
| 0 |
0 |
3 |
5 |
4 |
NM_078921.2 |
CG4485-RA (Cyp9b1), mRNA |
| 0 |
0 |
3 |
4 |
14 |
NM_143717.1 |
CG10840-RB (eIF5B), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_079877.2 |
CG17245-RA (plexB), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NR_001596.1 |
CR32011, mRNA |
| 0 |
0 |
3 |
3 |
2 |
NR_001597.1 |
CR32010, mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_170262.1 |
CG6058-RB, transcript variant B (Ald), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_170263.1 |
CG6058-RC, transcript variant C (Ald), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_170261.1 |
CG6058-RF, transcript variant F (Ald), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_170264.1 |
CG6058-RD, transcript variant D (Ald), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_206577.1 |
CG6058-RH, transcript variant H (Ald), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_170266.2 |
CG6058-RG, transcript variant G (Ald), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_170265.2 |
CG6058-RA, transcript variant A (Ald), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_079791.3 |
CG6058-RE, transcript variant E (Ald), mRNA |
| 0 |
0 |
2 |
9 |
40 |
NM_080025.1 |
CG11387-RA, transcript variant A (ct), mRNA |
| 0 |
0 |
2 |
5 |
13 |
NM_001014582.1 |
CG10488-RB, transcript variant B (eyg), mRNA |
| 0 |
0 |
2 |
5 |
13 |
NM_079318.2 |
CG10488-RA, transcript variant A (eyg), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_167852.1 |
CG9097-RB, transcript variant B (bab1), mRNA |
| 0 |
0 |
2 |
4 |
8 |
NM_206229.1 |
CG9097-RA, transcript variant A (bab1), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_134494.2 |
CG12235-RA (Arp11), mRNA |
| 0 |
0 |
2 |
3 |
12 |
NM_057432.2 |
CG2762-RA (ush), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_143362.2 |
CG14064-RA (beat-VI), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_139936.1 |
CG13678-RA (CG13678), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| + |
|
| DC |
|
| + |
|
| DM |
|
| + |
|
| VM |
|
| ++ |
|
| VB |
|
| ++ |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme00190
:
Oxidative phosphorylation
dme01100
:
Metabolic pathways
|
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