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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8114R-1

Symbol

pbl

Full Name

pebble

CG No

CG8114

Old CG No

CG8114

Synonyms

pebble, CG8114, PBL, l(3)09645, 0542/03, 0293/09, 0083/20, anon-WO0172774.25, anon-WO0172774.22, pbl, PEBBLE

Accession No (Link to NCBI)

NM_168241.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTGAAGAGCAATCGAAGTGCG

Primer Seq. 3'

aatctagaggtaccCCGAAAGGTTTTGGCGTACTG

Predicted Fragment Size

IR fragment full Seq

0001 TTGAAGAGCA ATCGAAGTGC GAGATGTCCA TAACAACGCT GCCCACGCGC ATCTGCCTGG 
0061 TGGGAGGCGT TGGCCAGGAT GCGGACACgC TGCAGGCAGC CGAATCCTTT GGCCTGCCCA 
0121 TCGTTACGTC GGACACGGGT CTGGACATTC TCGGAGAGTC GTCGGACTGG CGGACATTCT 
0181 ACGTGCTGGA TGACTTCGAG GGTGCCAGCT TCGAGGCGAT ACACAAGCAA AAGGAATGCA 
0241 TTCTGGGACC GCCGGCTTTG AAGTACGCGG CCGAGATGAA ACAGACACTG GGCCAGAACT 
0301 CGCGGCCCAT TTACAATTAC GCGATGCGCG GCGTGGTCAC TTGCTTTACA GGCATACGCA 
0361 AGAAGGATGA GCTGACAAAG CTGGTCAATC TCATTCACTC GATGGGTGGC TGCATCAAGA 
0421 AGGACCTGAA CACGAAGACC ACGCATTTGA TTTGCAACCA CAGTGGCGGC GAAAAGTACC 
0481 AGTACGCCAA AACCTTTCGG

in silico PCR Fragment

0001 TTGAAGAGCA ATCGAAGTGC GAGATGTCCA TAACAACGCT GCCCACGCGC ATCTGCCTGG 
0061 TGGGAGGCGT TGGCCAGGAT GCGGACACGC TGCAGGCAGC CGAATCCTTT GGCCTGCCCA 
0121 TCGTTACGTC GGACACGGGT CTGGACATTC TCGGAGAGTC GTCGGACTGG CGGACATTCT 
0181 ACGTGCTGGA TGACTTCGAG GGTGCCAGCT TCGAGGCGAT ACACAAGCAA AAGGAATGCA 
0241 TTCTGGGACC GCCGGCTTTG AAGTACGCGG CCGAGATGAA ACAGACACTG GGCCAGAACT 
0301 CGCGGCCCAT TTACAATTAC GCGATGCGCG GCGTGGTCAC TTGTTTTACA GGCATACGCA 
0361 AGAAGGATGA GCTGACAAAG CTGGTCAATC TCATTCACTC GATGGGTGGC TGCATCAAGA 
0421 AGGACCTGAA CACGAAGACC ACGCATTTGA TTTGCAACCA CAGTGGCGGC GAAAAGTACC 
0481 AGTACGCCAA AACCTTTCGG

Assemble Data

<PRE>

8114R-1.IR_full       1   TTGAAGAGCAATCGAAGTGCGAGATGTCCATAACAACGCTGCCCACGCGCATCTGCCTGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8114R-1.in silico     1   TTGAAGAGCAATCGAAGTGCGAGATGTCCATAACAACGCTGCCCACGCGCATCTGCCTGG 60


8114R-1.IR_full       61  TGGGAGGCGTTGGCCAGGATGCGGACACGCTGCAGGCAGCCGAATCCTTTGGCCTGCCCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8114R-1.in silico     61  TGGGAGGCGTTGGCCAGGATGCGGACACGCTGCAGGCAGCCGAATCCTTTGGCCTGCCCA 120


8114R-1.IR_full       121 TCGTTACGTCGGACACGGGTCTGGACATTCTCGGAGAGTCGTCGGACTGGCGGACATTCT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8114R-1.in silico     121 TCGTTACGTCGGACACGGGTCTGGACATTCTCGGAGAGTCGTCGGACTGGCGGACATTCT 180


8114R-1.IR_full       181 ACGTGCTGGATGACTTCGAGGGTGCCAGCTTCGAGGCGATACACAAGCAAAAGGAATGCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8114R-1.in silico     181 ACGTGCTGGATGACTTCGAGGGTGCCAGCTTCGAGGCGATACACAAGCAAAAGGAATGCA 240


8114R-1.IR_full       241 TTCTGGGACCGCCGGCTTTGAAGTACGCGGCCGAGATGAAACAGACACTGGGCCAGAACT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8114R-1.in silico     241 TTCTGGGACCGCCGGCTTTGAAGTACGCGGCCGAGATGAAACAGACACTGGGCCAGAACT 300


8114R-1.IR_full       301 CGCGGCCCATTTACAATTACGCGATGCGCGGCGTGGTCACTTG<span class="snp"><tt>C</tt></span>TTTACAGGCATACGCA 360
                          |||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||
8114R-1.in silico     301 CGCGGCCCATTTACAATTACGCGATGCGCGGCGTGGTCACTTG<span class="snp"><tt>T</tt></span>TTTACAGGCATACGCA 360


8114R-1.IR_full       361 AGAAGGATGAGCTGACAAAGCTGGTCAATCTCATTCACTCGATGGGTGGCTGCATCAAGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8114R-1.in silico     361 AGAAGGATGAGCTGACAAAGCTGGTCAATCTCATTCACTCGATGGGTGGCTGCATCAAGA 420


8114R-1.IR_full       421 AGGACCTGAACACGAAGACCACGCATTTGATTTGCAACCACAGTGGCGGCGAAAAGTACC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8114R-1.in silico     421 AGGACCTGAACACGAAGACCACGCATTTGATTTGCAACCACAGTGGCGGCGAAAAGTACC 480


8114R-1.IR_full       481 AGTACGCCAAAACCTTTCGG 500
                          ||||||||||||||||||||
8114R-1.in silico     481 AGTACGCCAAAACCTTTCGG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_168241.1	CG8114-RA, transcript variant A (pbl), mRNA
100	482	0	0	2	NM_079241.2	CG8114-RB, transcript variant B (pbl), mRNA
50.62	244	1	3	2	NM_168242.1	CG8114-RC, transcript variant C (pbl), mRNA
50.62	244	1	3	2	NM_168243.1	CG8114-RD, transcript variant D (pbl), mRNA
0	0	6	2	3	NM_144384.2	CG2958-RA (lectin-24Db), mRNA
0	0	5	2	2	NM_131920.2	CG6379-RA (CG6379), mRNA
0	0	2	3	5	NM_142387.1	CG7643-RA (ald), mRNA
0	0	1	6	5	NM_132644.1	CG1622-RA (CG1622), mRNA
0	0	1	5	2	NM_133134.2	CG12359-RA (Ulp1), mRNA
0	0	1	2	5	NM_132488.3	CG11727-RB, transcript variant B (CG11727), mRNA
0	0	1	2	5	NM_167285.2	CG11727-RA, transcript variant A (CG11727), mRNA
0	0	1	2	3	NM_141083.2	CG11306-RA (CG11306), mRNA
0	0	1	2	2	NM_140926.1	CG13811-RA (CG13811), mRNA
0	0	0	6	3	NM_130513.2	CG3711-RA, transcript variant A (CG3711), mRNA
0	0	0	6	3	NM_001038730.1	CG3711-RB, transcript variant B (CG3711), mRNA
0	0	0	5	4	NM_134552.3	CG1412-RA (RhoGAP19D), mRNA
0	0	0	5	2	NM_165153.1	CG31817-RA (CG31817), mRNA
0	0	0	4	11	NM_137151.2	CG10212-RA (SMC2), mRNA
0	0	0	4	10	NM_139892.1	CG12262-RA (CG12262), mRNA
0	0	0	4	8	NM_205960.1	CG4705-RB, transcript variant B (CG4705), mRNA
0	0	0	4	8	NM_135641.2	CG4705-RA, transcript variant A (CG4705), mRNA
0	0	0	4	5	NM_142957.1	CG13605-RA (CG13605), mRNA
0	0	0	4	4	NM_166707.1	CG9380-RB, transcript variant B (CG9380), mRNA
0	0	0	4	4	NM_001043106.1	CG9380-RC, transcript variant C (CG9380), mRNA
0	0	0	4	4	NM_058115.3	CG2859-RA (Taf10), mRNA
0	0	0	4	3	NM_137251.2	CG8443-RA (CG8443), mRNA
0	0	0	4	3	NM_078872.4	CG10302-RA (bsf), mRNA
0	0	0	4	3	NM_135640.1	CG16854-RA (CG16854), mRNA
0	0	0	4	3	NM_057953.3	CG3998-RA (zf30C), mRNA
0	0	0	3	10	NM_140007.1	CG5747-RA (mfr), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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