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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8098R-2

Symbol

Picot

Full Name

Picot

CG No

CG8098

Old CG No

CG8098

Synonyms

picot, CG8098, Picot

Accession No (Link to NCBI)

NM_137302.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCCCTCTTCGCATCCCACTAT

Primer Seq. 3'

aatctagaggtaccGGTCCAAGTGGTTGATCTTGA

Predicted Fragment Size

IR fragment full Seq

0001 TCCCTCTTCG CATCCCACTA TTGATGAGCG CGAGAAGAAG TACATTAACG ATTCCCTCTG 
0061 GGGAACTGAT GTTGTTAAGA GCCCTCCCAT TCCCTTCAAG GCTATCATCA AGTCGCTGCC 
0121 CTTCTACGCC ATTCTGTTCG CCCACATGGG TCACAACTAC GGCTATGAGA CTCTGATGAC 
0181 CGAGCTGCCC ACCTACATGA AGCAGGTGCT CCGCNTTCTC CCTGAAGTCG AACGGTCTGC 
0241 TCAGCTCCCT GCCCTACTTG GCCATGTGGC TCTNTCTCCA TGTTCATCTC GGTGGTCGCC 
0301 GATTGGATGA TTAGCTCGAA GAGATTCTCN GCACACGGCC ACCAGGAAGC TGATCAACAG 
0361 TATTGGCCAG TATGGACCGG GAGTCGCCCT AATCGCCGCC TCCTACACGG GCTGCGACAG 
0421 AGCACTGACC TTGGCCATCC TCACCATCGG AGTGGGTCTT AATGGAGGTA TCTACTCCGG 
0481 CTTCAAGATC AACCACTTGG ACC

in silico PCR Fragment

0001 TCCCTCTTCG CATCCCACTA TTGATGAGCG CGAGAAGAAG TACATTAACG ATTCCCTCTG 
0061 GGGAACTGAT GTTGTTAAGA GCCCTCCCAT TCCCTTCAAG GCTATCATCA AGTCGCTGCC 
0121 CTTCTACGCC ATTCTGTTCG CCCACATGGG TCACAACTAC GGCTATGAGA CTCTGATGAC 
0181 CGAGCTGCCC ACCTACATGA AGCAGGTGCT CCGC-TTCTC CCTGAAGTCG AACGGTCTGC 
0241 TCAGCTCCCT GCCCTACTTG GCCATGTGGC TCT-TCTCCA TGTTCATCTC GGTGGTCGCC 
0301 GATTGGATGA TTAGCTCGAA GAGATTCTC- GCACACGGCC ACCAGGAAGC TGATCAACAG 
0361 TATTGGCCAG TATGGACCGG GAGTCGCCCT AATCGCCGCC TCCTACACGG GCTGCGACAG 
0421 AGCACTGACC TTGGCCATCC TCACCATCGG AGTGGGTCTT AATGGAGGTA TCTACTCCGG 
0481 CTTCAAGATC AACCACTTGG ACC

Assemble Data

<PRE>

8098R-2.IR_full       1   TCCCTCTTCGCATCCCACTATTGATGAGCGCGAGAAGAAGTACATTAACGATTCCCTCTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8098R-2.in silico     1   TCCCTCTTCGCATCCCACTATTGATGAGCGCGAGAAGAAGTACATTAACGATTCCCTCTG 60


8098R-2.IR_full       61  GGGAACTGATGTTGTTAAGAGCCCTCCCATTCCCTTCAAGGCTATCATCAAGTCGCTGCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8098R-2.in silico     61  GGGAACTGATGTTGTTAAGAGCCCTCCCATTCCCTTCAAGGCTATCATCAAGTCGCTGCC 120


8098R-2.IR_full       121 CTTCTACGCCATTCTGTTCGCCCACATGGGTCACAACTACGGCTATGAGACTCTGATGAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8098R-2.in silico     121 CTTCTACGCCATTCTGTTCGCCCACATGGGTCACAACTACGGCTATGAGACTCTGATGAC 180


8098R-2.IR_full       181 CGAGCTGCCCACCTACATGAAGCAGGTGCTCCGC<span class="snp"><tt>N</tt></span>TTCTCCCTGAAGTCGAACGGTCTGC 240
                          ||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||
8098R-2.in silico     181 CGAGCTGCCCACCTACATGAAGCAGGTGCTCCGC<span class="snp"><tt>-</tt></span>TTCTCCCTGAAGTCGAACGGTCTGC 240


8098R-2.IR_full       241 TCAGCTCCCTGCCCTACTTGGCCATGTGGCTCT<span class="snp"><tt>N</tt></span>TCTCCATGTTCATCTCGGTGGTCGCC 300
                          |||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||
8098R-2.in silico     241 TCAGCTCCCTGCCCTACTTGGCCATGTGGCTCT<span class="snp"><tt>-</tt></span>TCTCCATGTTCATCTCGGTGGTCGCC 300


8098R-2.IR_full       301 GATTGGATGATTAGCTCGAAGAGATTCTC<span class="snp"><tt>N</tt></span>GCACACGGCCACCAGGAAGCTGATCAACAG 360
                          |||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||
8098R-2.in silico     301 GATTGGATGATTAGCTCGAAGAGATTCTC<span class="snp"><tt>-</tt></span>GCACACGGCCACCAGGAAGCTGATCAACAG 360


8098R-2.IR_full       361 TATTGGCCAGTATGGACCGGGAGTCGCCCTAATCGCCGCCTCCTACACGGGCTGCGACAG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8098R-2.in silico     361 TATTGGCCAGTATGGACCGGGAGTCGCCCTAATCGCCGCCTCCTACACGGGCTGCGACAG 420


8098R-2.IR_full       421 AGCACTGACCTTGGCCATCCTCACCATCGGAGTGGGTCTTAATGGAGGTATCTACTCCGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8098R-2.in silico     421 AGCACTGACCTTGGCCATCCTCACCATCGGAGTGGGTCTTAATGGAGGTATCTACTCCGG 480


8098R-2.IR_full       481 CTTCAAGATCAACCACTTGGACC 503
                          |||||||||||||||||||||||
8098R-2.in silico     481 CTTCAAGATCAACCACTTGGACC 503

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_137302.2	CG8098-RA, transcript variant A (Picot), mRNA
100	482	0	0	0	NM_166187.1	CG8098-RB, transcript variant B (Picot), mRNA
0.82	4	3	3	6	NM_132629.1	CG4330-RA (CG4330), mRNA
0.2	1	2	7	18	NM_134728.1	CG4726-RA (CG4726), mRNA
0	0	8	19	28	NM_131960.1	CG6978-RA (CG6978), mRNA
0	0	5	6	9	NM_206202.1	CG9826-RB, transcript variant B (CG9826), mRNA
0	0	5	6	9	NM_137880.1	CG9826-RA, transcript variant A (CG9826), mRNA
0	0	4	2	4	NM_078660.2	CG5424-RB, transcript variant B (f), mRNA
0	0	4	2	4	NM_001042819.1	CG5424-RF, transcript variant F (f), mRNA
0	0	4	2	4	NM_176745.1	CG5424-RD, transcript variant D (f), mRNA
0	0	4	2	4	NM_176746.1	CG5424-RC, transcript variant C (f), mRNA
0	0	4	2	4	NM_176747.1	CG5424-RE, transcript variant E (f), mRNA
0	0	4	2	4	NM_167563.2	CG5424-RA, transcript variant A (f), mRNA
0	0	3	7	5	NM_057376.2	CG7734-RC, transcript variant C (shn), mRNA
0	0	3	7	5	NM_134279.2	CG7734-RB, transcript variant B (shn), mRNA
0	0	3	7	5	NM_057375.3	CG7734-RA, transcript variant A (shn), mRNA
0	0	3	7	5	NM_206091.1	CG7734-RD, transcript variant D (shn), mRNA
0	0	2	3	5	NM_142560.2	CG17752-RA (CG17752), mRNA
0	0	2	3	2	NM_141784.2	CG6629-RA (CG6629), mRNA
0	0	1	7	5	NM_135767.1	CG5682-RA (CG5682), mRNA
0	0	1	6	5	NM_133013.1	CG15816-RA (CG15816), mRNA
0	0	1	5	4	NM_131924.2	CG4857-RB (CG4857), mRNA
0	0	1	4	5	NM_136652.2	CG1809-RA (CG1809), mRNA
0	0	1	4	5	NM_079060.2	CG17725-RA, transcript variant A (Pepck), mRNA
0	0	1	4	5	NM_166293.1	CG17725-RB, transcript variant B (Pepck), mRNA
0	0	1	3	2	NM_136426.2	CG1707-RA (CG1707), mRNA
0	0	1	2	5	NM_080317.2	CG2647-RA (per), mRNA
0	0	1	2	5	NM_165181.1	CG17927-RC, transcript variant C (Mhc), mRNA
0	0	1	2	5	NM_165182.1	CG17927-RG, transcript variant G (Mhc), mRNA
0	0	1	2	5	NM_165183.1	CG17927-RE, transcript variant E (Mhc), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme04142 : Lysosome

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