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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8040R-1 
 Symbol CG32061  Full Name CG32061 
 CG No CG32061  Old CG No CG8040 
 Synonyms CG8040, CG32061 
 Accession No (Link to NCBI) NM_168409.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
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 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTCGAGGCGAATGCCAATCTAA 
 Primer Seq. 3' 
aatctagaggtaccGGATACTCCTCCGCCGATAAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TCGAGGCGAA tgNCANTCTA AAAACGGTCT ATCCCTCCGT GAATCGTAAT CTAACGCTTC 
0061 AATTGGGCTA TAAATACAAT CACTGGATGC TGGTTAAGGC CATAATGTAT TCACTACTAT 
0121 CTATGATCGG AGTTTGGTAC TCACGACACT TGCAAAGTGG CAAAAGTGAG GTATCGATGG 
0181 AAAAGAAAGG AATCCTTAAA ACTACGACAA CAGCTGCAGA GCAAGTGAAA AACAATTGGA 
0241 TCAAAAAACA GAATTCCGCA TTGAAAAACG GAACCCTGCC GCGGAATATA TTCCTGTTAT 
0301 TTGCTTTGCC CTGGATATTG ACCTTTGTGG CCAGCGGATT GATCAACTAT ATTCGATTTT 
0361 ATATGGTCAT TCAGCATTTT TGCATATCTA ACTGGAGGGC GATTCAGCTT TATGGAGAAC 
0421 TGCAAGGCCT CTTTTGGCAT CGTTCTACAN CCGTGACTTT AATACCCTAT TGGTCGCAAA 
0481 TTATCGGCGG AGGAGTATCC  
 in silico PCR Fragment 
0001 TCGAGGCGAA TGCCAATCTA AAAACGGTCT ATCCCTCCGT GAATCGTAAT CTAACGCTTC 
0061 AATTGGGCTA TAAATACAAT CACTGGATGC TGGTTAAGGC CATAATGTAT TCACTACTAT 
0121 CTATGATCGG AGTTTGGTAC TCACGACACT TGCAAAGTGG CAAAAGTGAG GTATCGATGG 
0181 AAAAGAAAGG AATCCTTAAA ACTACGACAA CAGCTGCAGA GCAAGTGAAA AACAATTGGA 
0241 TCAAAAAACA GAATTCCGCA TTGAAAAACG GAACCCTGCC GCGGAATATA TTCCTGTTAT 
0301 TTGCTTTGCC CTGGATATTG ACCTTTGTGG CCAGCGGATT GATCAACTAT ATTCGATTTT 
0361 ACATGGTCAT ACAGCATTTT TGCATATCTA ACTGGAGGGC GATTCAGCTT TATGGAGAAC 
0421 TGCAAGGCCT CTTTTGGCAT CGTTCTACAT CCGTGACTTT AATACCCTAT TGGTCGCAAA 
0481 TTATCGGCGG AGGAGTATCC  
 Assemble Data 
<PRE>

8040R-1.IR_full       1   TCGAGGCGAATG<span class="snp"><tt>N</tt></span>CA<span class="snp"><tt>N</tt></span>TCTAAAAACGGTCTATCCCTCCGTGAATCGTAATCTAACGCTTC 60
                          ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||
8040R-1.in silico     1   TCGAGGCGAATG<span class="snp"><tt>C</tt></span>CA<span class="snp"><tt>A</tt></span>TCTAAAAACGGTCTATCCCTCCGTGAATCGTAATCTAACGCTTC 60


8040R-1.IR_full       61  AATTGGGCTATAAATACAATCACTGGATGCTGGTTAAGGCCATAATGTATTCACTACTAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8040R-1.in silico     61  AATTGGGCTATAAATACAATCACTGGATGCTGGTTAAGGCCATAATGTATTCACTACTAT 120


8040R-1.IR_full       121 CTATGATCGGAGTTTGGTACTCACGACACTTGCAAAGTGGCAAAAGTGAGGTATCGATGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8040R-1.in silico     121 CTATGATCGGAGTTTGGTACTCACGACACTTGCAAAGTGGCAAAAGTGAGGTATCGATGG 180


8040R-1.IR_full       181 AAAAGAAAGGAATCCTTAAAACTACGACAACAGCTGCAGAGCAAGTGAAAAACAATTGGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8040R-1.in silico     181 AAAAGAAAGGAATCCTTAAAACTACGACAACAGCTGCAGAGCAAGTGAAAAACAATTGGA 240


8040R-1.IR_full       241 TCAAAAAACAGAATTCCGCATTGAAAAACGGAACCCTGCCGCGGAATATATTCCTGTTAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8040R-1.in silico     241 TCAAAAAACAGAATTCCGCATTGAAAAACGGAACCCTGCCGCGGAATATATTCCTGTTAT 300


8040R-1.IR_full       301 TTGCTTTGCCCTGGATATTGACCTTTGTGGCCAGCGGATTGATCAACTATATTCGATTTT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8040R-1.in silico     301 TTGCTTTGCCCTGGATATTGACCTTTGTGGCCAGCGGATTGATCAACTATATTCGATTTT 360


8040R-1.IR_full       361 A<span class="snp"><tt>T</tt></span>ATGGTCAT<span class="snp"><tt>T</tt></span>CAGCATTTTTGCATATCTAACTGGAGGGCGATTCAGCTTTATGGAGAAC 420
                          |<span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
8040R-1.in silico     361 A<span class="snp"><tt>C</tt></span>ATGGTCAT<span class="snp"><tt>A</tt></span>CAGCATTTTTGCATATCTAACTGGAGGGCGATTCAGCTTTATGGAGAAC 420


8040R-1.IR_full       421 TGCAAGGCCTCTTTTGGCATCGTTCTACA<span class="snp"><tt>N</tt></span>CCGTGACTTTAATACCCTATTGGTCGCAAA 480
                          |||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||
8040R-1.in silico     421 TGCAAGGCCTCTTTTGGCATCGTTCTACA<span class="snp"><tt>T</tt></span>CCGTGACTTTAATACCCTATTGGTCGCAAA 480


8040R-1.IR_full       481 TTATCGGCGGAGGAGTATCC 500
                          ||||||||||||||||||||
8040R-1.in silico     481 TTATCGGCGGAGGAGTATCC 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_168409.1  CG32061-RA (CG32061), mRNA 
0   0  2  3  2  NM_166317.1  CG18608-RA (prod), mRNA 
0   0  2  3  2  NM_001015350.1  CG40085-PA.3 (CG40085), mRNA 
0   0  2  2  2  NM_166986.2  CG2849-RA, transcript variant A (Rala), mRNA 
0   0  2  2  2  NM_080324.3  CG2849-RC, transcript variant C (Rala), mRNA 
0   0  2  2  2  NM_166985.2  CG2849-RB, transcript variant B (Rala), mRNA 
0   0  1  3  3  NM_165991.1  CG4832-RC, transcript variant C (cnn), mRNA 
0   0  1  3  3  NM_001014523.1  CG4832-RD, transcript variant D (cnn), mRNA 
0   0  1  3  3  NM_144328.2  CG10570-RA (CG10570), mRNA 
0   0  1  3  2  NM_057736.3  CG4832-RA, transcript variant A (cnn), mRNA 
0   0  1  3  2  NM_001038855.1  CG4832-RE, transcript variant E (cnn), mRNA 
0   0  1  3  2  NM_165990.1  CG4832-RB, transcript variant B (cnn), mRNA 
0   0  1  3  2  NM_168492.1  CG32099-RA (eap), mRNA 
0   0  0  5  6  NM_164491.2  CG17302-RA (CG17302), mRNA 
0   0  0  5  2  NM_135904.1  CG15263-RA (CG15263), mRNA 
0   0  0  5  2  NM_166811.1  CG18069-RB, transcript variant B (CaMKII), mRNA 
0   0  0  5  2  NM_166810.3  CG18069-RA, transcript variant A (CaMKII), mRNA 
0   0  0  5  2  NM_079896.3  CG18069-RC, transcript variant C (CaMKII), mRNA 
0   0  0  5  2  NM_166812.2  CG18069-RD, transcript variant D (CaMKII), mRNA 
0   0  0  5  2  NM_166813.2  CG18069-RE, transcript variant E (CaMKII), mRNA 
0   0  0  5  2  NM_001014696.1  CG18069-RG, transcript variant G (CaMKII), mRNA 
0   0  0  4  9  NM_142220.1  CG6045-RA (CG6045), mRNA 
0   0  0  4  6  NM_166353.1  CG10023-RC, transcript variant C (Fak56D), mRNA 
0   0  0  4  6  NM_166352.1  CG10023-RB, transcript variant B (Fak56D), mRNA 
0   0  0  4  6  NM_079069.2  CG10023-RA, transcript variant A (Fak56D), mRNA 
0   0  0  4  6  NM_140363.2  CG11261-RA (CG11261), mRNA 
0   0  0  4  6  NM_165747.2  CG4001-RC, transcript variant C (Pfk), mRNA 
0   0  0  4  6  NM_165746.1  CG4001-RB, transcript variant B (Pfk), mRNA 
0   0  0  3  9  NM_137003.3  CG4630-RA (CG4630), mRNA 
0   0  0  3  4  NM_164896.1  CG31720-RA (CG31720), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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