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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8025R-2 |
| Symbol |
Mtr3 |
Full Name |
Mtr3 |
| CG No |
CG8025 |
Old CG No |
CG8025 |
| Synonyms |
CG8025, dMtr3, Mtr3 |
| Accession No (Link to NCBI) |
NM_140900.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGATTCGGTGCCGGAACGGGA |
| Primer Seq. 3' |
aatctagaggtaccCCGCTTCGTGAGCACGCCTTT |
| Predicted Fragment Size |
496 |
| IR fragment full Seq |
0001 AGATTCGGTG CCGGAAcNGG AACGCCACCT GAGCAGCATG CTGACCAAGG CCATGGAGCC
0061 GGNTGGTGTG CCGCACGGAG TTCCTTAACT TTCAGCTGGA CATACGGGTG CTTATTCTCG
0121 ACGATGACGG CTGCCTGCTC AGCACTGCCA TCAATTGCTG CGGAGTCGCT TTGGTGGAGT
0181 GCGGCATTTC CACTTACGAC TTAATTACGG CCTCCACAGC TTGTATCTAT CGGGATCACG
0241 TCTTCCTGAA TCCCAGTGCC AAGGTTGAGG AGCTGCTCTG GAAGCATCGC AACAGCAGCA
0301 CCGATAGCAC AACCAGCCCA TCATCCGCCC AGGAGCACGG CCTGATCATT ACCGCCAGCA
0361 TGGATACCTT TGAACAGATC GCCCAGTGCC AGCAGTGCGG ATATCTCAGT CCAGCCACCT
0421 ATGTAAAGCT GCTGGATTAC ACCCTGGCCA TTAACAAGTC CCTGAGAGAA CTAGTCAAAG
0481 GCGTGCTCAC GANGCNG |
| in silico PCR Fragment |
0001 AGATTCGGTG CCGGAACGGG AACGCCACCT GAGCAGCATG CTGACCAAGG CCATGGAGCC
0061 GG-TGGTGTG CCGCACGGAG TTCCTTAACT TTCAGCTGGA CATACGGGTG CTTATTCTCG
0121 ACGATGACGG CTGCCTGCTC AGCACTGCCA TCAATTGCTG CGGAGTCGCT TTGGTGGAGT
0181 GCGGCATTTC CACTTACGAC TTAATTACGG CCTCCACAGC TTGTATCTAT CGGGATCACG
0241 TCTTCCTGAA TCCCAGTGCC AAGGTTGAGG AGCTGCTCTG GAAGCATCGC AACAGCAGCA
0301 CCGATAGCAC AACCAGCCCA TCATCCGCCC AGGAGCACGG CCTGATCATT ACCGCCAGCA
0361 TGGATACCTT TGAACAGATC GCCCAGTGCC AGCAGTGCGG ATATCTCAGT CCAGCCACCT
0421 ATGTAAAGCT GCTGGATTAC ACCCTGGCCA TTAACAAGTC CCTGAGAGAA CTAGTCAAAG
0481 GCGTGCTCAC GAAGCGG |
| Assemble Data |
8025R-2.IR_full 1 AGATTCGGTGCCGGAACNGGAACGCCACCTGAGCAGCATGCTGACCAAGGCCATGGAGCC 60
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
8025R-2.in silico 1 AGATTCGGTGCCGGAACGGGAACGCCACCTGAGCAGCATGCTGACCAAGGCCATGGAGCC 60
8025R-2.IR_full 61 GGNTGGTGTGCCGCACGGAGTTCCTTAACTTTCAGCTGGACATACGGGTGCTTATTCTCG 120
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8025R-2.in silico 61 GG-TGGTGTGCCGCACGGAGTTCCTTAACTTTCAGCTGGACATACGGGTGCTTATTCTCG 120
8025R-2.IR_full 121 ACGATGACGGCTGCCTGCTCAGCACTGCCATCAATTGCTGCGGAGTCGCTTTGGTGGAGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8025R-2.in silico 121 ACGATGACGGCTGCCTGCTCAGCACTGCCATCAATTGCTGCGGAGTCGCTTTGGTGGAGT 180
8025R-2.IR_full 181 GCGGCATTTCCACTTACGACTTAATTACGGCCTCCACAGCTTGTATCTATCGGGATCACG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8025R-2.in silico 181 GCGGCATTTCCACTTACGACTTAATTACGGCCTCCACAGCTTGTATCTATCGGGATCACG 240
8025R-2.IR_full 241 TCTTCCTGAATCCCAGTGCCAAGGTTGAGGAGCTGCTCTGGAAGCATCGCAACAGCAGCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8025R-2.in silico 241 TCTTCCTGAATCCCAGTGCCAAGGTTGAGGAGCTGCTCTGGAAGCATCGCAACAGCAGCA 300
8025R-2.IR_full 301 CCGATAGCACAACCAGCCCATCATCCGCCCAGGAGCACGGCCTGATCATTACCGCCAGCA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8025R-2.in silico 301 CCGATAGCACAACCAGCCCATCATCCGCCCAGGAGCACGGCCTGATCATTACCGCCAGCA 360
8025R-2.IR_full 361 TGGATACCTTTGAACAGATCGCCCAGTGCCAGCAGTGCGGATATCTCAGTCCAGCCACCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8025R-2.in silico 361 TGGATACCTTTGAACAGATCGCCCAGTGCCAGCAGTGCGGATATCTCAGTCCAGCCACCT 420
8025R-2.IR_full 421 ATGTAAAGCTGCTGGATTACACCCTGGCCATTAACAAGTCCCTGAGAGAACTAGTCAAAG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8025R-2.in silico 421 ATGTAAAGCTGCTGGATTACACCCTGGCCATTAACAAGTCCCTGAGAGAACTAGTCAAAG 480
8025R-2.IR_full 481 GCGTGCTCACGANGCNG 497
|||||||||||| || |
8025R-2.in silico 481 GCGTGCTCACGAAGCGG 497
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
478 |
0 |
0 |
0 |
NM_140900.2 |
CG8025-RA (Mtr3), mRNA |
| 0.2 |
1 |
3 |
2 |
3 |
NM_001014640.1 |
CG14299-RB, transcript variant B (CG14299), mRNA |
| 0.2 |
1 |
3 |
2 |
3 |
NM_142495.1 |
CG14299-RA, transcript variant A (CG14299), mRNA |
| 0.2 |
1 |
2 |
3 |
8 |
NM_168201.2 |
CG8598-RB, transcript variant B (eco), mRNA |
| 0.2 |
1 |
2 |
3 |
8 |
NM_139849.2 |
CG8598-RA, transcript variant A (eco), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_176737.1 |
CG9213-RA (CG9213), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_142044.2 |
CG9588-RA (CG9588), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_001038726.1 |
CG17131-RB, transcript variant B (SP71), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_001031861.1 |
CG17131-RA, transcript variant A (SP71), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_141169.2 |
CG10712-RA, transcript variant A (Chro), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_168976.1 |
CG10712-RC, transcript variant C (Chro), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_168975.1 |
CG10712-RB, transcript variant B (Chro), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_170564.1 |
CG1856-RA, transcript variant A (ttk), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_170565.1 |
CG1856-RB, transcript variant B (ttk), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_170566.1 |
CG1856-RE, transcript variant E (ttk), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_137212.2 |
CG30089-RA (CG30089), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_176542.1 |
CG7029-RA (CG7029), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_140174.4 |
CG7839-RA (CG7839), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132216.2 |
CG2116-RA (CG2116), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_079586.2 |
CG6598-RA (Fdh), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137674.1 |
CG9993-RA (CG9993), mRNA |
| 0 |
0 |
1 |
3 |
11 |
NM_166057.1 |
CG8787-RA, transcript variant A (Asx), mRNA |
| 0 |
0 |
1 |
3 |
11 |
NM_001014527.1 |
CG8787-RB, transcript variant B (Asx), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_137998.2 |
CG11183-RA (Dcp1), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137763.3 |
CG17922-RA (CG17922), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136917.2 |
CG8487-RB, transcript variant B (garz), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165881.1 |
CG8487-RA, transcript variant A (garz), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_143367.2 |
CG10011-RA (CG10011), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_080161.1 |
CG3496-RA (vir), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140240.1 |
CG6053-RA (CG6053), mRNA |
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| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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| Dcompartment |
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Penetrance |
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- |
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