NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7879R-1

Symbol

CG7879

Full Name

CG7879

CG No

CG7879

Old CG No

CG7879

Synonyms

cg7879, CG7879

Accession No (Link to NCBI)

NM_139369.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.<br> A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.<br> Dev Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAATGCGCGCGACATTCGAAAC

Primer Seq. 3'

aatctagaggtaccTCCCGCTCCCTATCGCTATCA

Predicted Fragment Size

IR fragment full Seq

0001 AATGCGCGCG ACATTCGAAA CTTCTTCTCG GGCCTCTCAA TTCCCGAGGG CGGTGTCCAC 
0061 ATAATCGGCG GCGAGATGGG CGACGCTTTC ATCGCTTTCA GCACCGACGA GGATGCCCGC 
0121 TGCGCAATGC TGAAGGATCG CGAGAAACTG ATGGAGATCC AGGTGCGCCT GCTGCTCTCC 
0181 TCGCGGGCCG AGATGCAAAA GGTTATTGAG ACTGCTCGGA AAGCGGCGTC CgCTCAAGCG 
0241 CCAGTAGCGG TTCCAGTTCC TATGCCCGTT GTCGTCCCCG TTACGGCGGC AAAGGTGGCT 
0301 GGGTCCGGCT GCGCCACTGC CTCCGATTAT TGGGGGCCTA AACACCTTCT TGGGTCAAGG 
0361 TCAAGTCAAG GGTGTGGCCA CAGGGACAGT GCCCTCTTTT CTGACCTACC AACAGCAGGC 
0421 GGGGATCACG CTCTCTGATG TCACAGGTCG CCGCAGTCGC AGCAGATCGT CCAGTTCCGA 
0481 TGATAGCGAT AGGGAGCGGG A

in silico PCR Fragment

0001 AATGCGCGCG ACATTCGAAA CTTCTTCTCG GGCCTCTCAA TTCCCGAGGG CGGTGTCCAC 
0061 ATAATCGGCG GCGAGATGGG CGACGCTTTC ATCGCTTTCA GCACCGACGA GGATGCCCGC 
0121 TGCGCAATGC TGAAGGATCG CGAGAAACTA ATGGAGATCC AGGTGCGCCT GCTGCTATCC 
0181 TCGCGGGCCG AGATGCAAAA GGTTATTGAG ACTGCTCGGA AAGCGGCGTC CGCTCAAGCG 
0241 CCAGTATCGG TTCCAGTTCC TATGCCCGTT GTCGTCCCCG TTACGGCGGC AAAGGTGGCT 
0301 -GGTCCGGCT GCGCCACTGC CTCCGATTAT TGGGGGCCTA AACACCTTCT TGGGTCAAGG 
0361 TCAAGTCAAG GGTGTGGCCA CAGGGACAGT GCCCTCTTTT CTGACCTACC AACAGCAGGC 
0421 GGGGATCACG CTCTCTGATG TCACAGGTCG CCGCAGTCGC AGCAGATCGT CCAGTTCCGA 
0481 TGATAGCGAT AGGGAGCGGG A

Assemble Data

<PRE>

7879R-1.IR_full       1   AATGCGCGCGACATTCGAAACTTCTTCTCGGGCCTCTCAATTCCCGAGGGCGGTGTCCAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7879R-1.in silico     1   AATGCGCGCGACATTCGAAACTTCTTCTCGGGCCTCTCAATTCCCGAGGGCGGTGTCCAC 60


7879R-1.IR_full       61  ATAATCGGCGGCGAGATGGGCGACGCTTTCATCGCTTTCAGCACCGACGAGGATGCCCGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7879R-1.in silico     61  ATAATCGGCGGCGAGATGGGCGACGCTTTCATCGCTTTCAGCACCGACGAGGATGCCCGC 120


7879R-1.IR_full       121 TGCGCAATGCTGAAGGATCGCGAGAAACT<span class="snp"><tt>G</tt></span>ATGGAGATCCAGGTGCGCCTGCTGCT<span class="snp"><tt>C</tt></span>TCC 180
                          |||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||
7879R-1.in silico     121 TGCGCAATGCTGAAGGATCGCGAGAAACT<span class="snp"><tt>A</tt></span>ATGGAGATCCAGGTGCGCCTGCTGCT<span class="snp"><tt>A</tt></span>TCC 180


7879R-1.IR_full       181 TCGCGGGCCGAGATGCAAAAGGTTATTGAGACTGCTCGGAAAGCGGCGTCCGCTCAAGCG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7879R-1.in silico     181 TCGCGGGCCGAGATGCAAAAGGTTATTGAGACTGCTCGGAAAGCGGCGTCCGCTCAAGCG 240


7879R-1.IR_full       241 CCAGTA<span class="snp"><tt>G</tt></span>CGGTTCCAGTTCCTATGCCCGTTGTCGTCCCCGTTACGGCGGCAAAGGTGGCT 300
                          ||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
7879R-1.in silico     241 CCAGTA<span class="snp"><tt>T</tt></span>CGGTTCCAGTTCCTATGCCCGTTGTCGTCCCCGTTACGGCGGCAAAGGTGGCT 300


7879R-1.IR_full       301 <span class="snp"><tt>G</tt></span>GGTCCGGCTGCGCCACTGCCTCCGATTATTGGGGGCCTAAACACCTTCTTGGGTCAAGG 360
                          <span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7879R-1.in silico     301 <span class="snp"><tt>-</tt></span>GGTCCGGCTGCGCCACTGCCTCCGATTATTGGGGGCCTAAACACCTTCTTGGGTCAAGG 360


7879R-1.IR_full       361 TCAAGTCAAGGGTGTGGCCACAGGGACAGTGCCCTCTTTTCTGACCTACCAACAGCAGGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7879R-1.in silico     361 TCAAGTCAAGGGTGTGGCCACAGGGACAGTGCCCTCTTTTCTGACCTACCAACAGCAGGC 420


7879R-1.IR_full       421 GGGGATCACGCTCTCTGATGTCACAGGTCGCCGCAGTCGCAGCAGATCGTCCAGTTCCGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7879R-1.in silico     421 GGGGATCACGCTCTCTGATGTCACAGGTCGCCGCAGTCGCAGCAGATCGTCCAGTTCCGA 480


7879R-1.IR_full       481 TGATAGCGATAGGGAGCGGGA 501
                          |||||||||||||||||||||
7879R-1.in silico     481 TGATAGCGATAGGGAGCGGGA 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_167896.1	CG7879-RB, transcript variant B (CG7879), mRNA
100	482	0	0	2	NM_139369.1	CG7879-RA, transcript variant A (CG7879), mRNA
0.2	1	4	2	2	NM_139460.1	CG9004-RA (CG9004), mRNA
0.2	1	2	2	4	NM_134552.3	CG1412-RA (RhoGAP19D), mRNA
0	0	4	2	8	NM_143019.2	CG6695-RA, transcript variant A (CG6695), mRNA
0	0	4	2	8	NM_206565.1	CG6695-RB, transcript variant B (CG6695), mRNA
0	0	3	4	4	NM_001014582.1	CG10488-RB, transcript variant B (eyg), mRNA
0	0	3	4	4	NM_079318.2	CG10488-RA, transcript variant A (eyg), mRNA
0	0	2	1	1	NM_001042841.1	CG40500-RD, transcript variant D (CG40500), mRNA
0	0	2	1	1	NM_001042839.1	CG40500-RB, transcript variant B (CG40500), mRNA
0	0	2	1	1	NM_001042840.1	CG40500-RA, transcript variant A (CG40500), mRNA
0	0	2	1	1	NM_001042838.1	CG40500-RC, transcript variant C (CG40500), mRNA
0	0	1	4	5	NM_057849.3	CG3733-RA (Chd1), mRNA
0	0	1	4	2	NM_205884.1	CG4574-RC, transcript variant C (Plc21C), mRNA
0	0	1	4	2	NM_057504.4	CG4574-RA, transcript variant A (Plc21C), mRNA
0	0	1	4	2	NM_205883.1	CG4574-RD, transcript variant D (Plc21C), mRNA
0	0	1	4	2	NM_057503.4	CG4574-RB, transcript variant B (Plc21C), mRNA
0	0	1	4	2	NM_205882.1	CG4574-RF, transcript variant F (Plc21C), mRNA
0	0	1	3	4	NM_140097.1	CG6749-RA (CG6749), mRNA
0	0	1	3	4	NM_136368.2	CG17994-RA (CG17994), mRNA
0	0	1	2	7	NM_138220.1	CG13889-RA (CG13889), mRNA
0	0	1	2	3	NM_143000.1	CG17782-RA (CG17782), mRNA
0	0	1	2	3	NM_130512.2	CG11663-RA (CG11663), mRNA
0	0	1	2	3	NM_057820.3	CG9261-RC, transcript variant C (nrv2), mRNA
0	0	1	2	3	NM_001014475.1	CG9261-RF, transcript variant F (nrv2), mRNA
0	0	1	2	3	NM_136017.2	CG15151-RA (PFE), mRNA
0	0	1	2	2	NM_001038818.1	CG4551-RE, transcript variant E (smi35A), mRNA
0	0	1	2	2	NM_205988.2	CG4551-RC, transcript variant C (smi35A), mRNA
0	0	1	2	2	NM_078840.4	CG4551-RA, transcript variant A (smi35A), mRNA
0	0	1	2	2	NM_205989.2	CG4551-RB, transcript variant B (smi35A), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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