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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7823R-4

Symbol RhoGDI Full Name RhoGDI

CG No CG7823 Old CG No CG7823

Synonyms CG7823, RhoGDI

Accession No (Link to NCBI) NM_140905.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGAGACGAAGCACCATCCCGA

Primer Seq. 3'

aatctagaggtaccCGCGGGAGAATGTGCCTGAAG

Predicted Fragment Size

IR fragment full Seq

0001 AGAGACGAAG CACCATCCCG AGCACCACGA CGACGATGTC CACGATGCCA ACTACCAGGC 
0061 GCCGCCGGAG AAGACCATCG AGGAAATAAT GGCCGCCGAT CAGGAGGATG AGAGCTTGCG 
0121 GCGCTACAAG GAGGCGCTCC TGGGTGCCGC CCAGACCGAG AAAATTATTG TTGAACCCAA 
0181 TGATCCCCGA AAAGTTATTG TAAAGAAACT GGCCCTCGTT GTCGAAGGAC GCGACGACAT 
0241 GGAATTGGAT CTTACCGGTG ACCTTAGTCA GCTGAAAAAG CAGCTTTTTG TGATCAAAGA 
0301 AGGTGTTCAG TACAAGGTGC GCATTGATTT TATTGTGCAG CGTGAAATAG TCCATGGCCT 
0361 TAAGTATGTT CAAAAGACTT CTCGCTTGGG TGTTAATGTT GACAAGATGA AGCATATGGT 
0421 TGGCTCCTAT CCGCCGAAGA AGGAGATTCA GTTCTATTTG ACGCCCGCCG AGGAGGCACC 
0481 TTCAGGCACA TTNCNCCCGc G

in silico PCR Fragment

0001 AGAGACGAAG CACCATCCCG AGCACCACGA CGACGATGTC CACGATGCCA ACTACCAGGC 
0061 GCCGCCGGAG AAGACCATCG AGGAAATAAT GGCCGCCGAT CAGGAGGATG AGAGCTTGCG 
0121 GCGCTACAAG GAGGCGCTCC TGGGTGCCGC CCAGACCGAG AAAATTATTG TTGAACCCAA 
0181 TGATCCCCGA AAAGTTATTG TAAAGAAACT GGCCCTCGTT GTCGAAGGAC GCGACGACAT 
0241 GGAATTGGAT CTTACCGGTG ACCTTAGTCA GCTGAAAAAG CAGCTTTTTG TGATCAAAGA 
0301 AGGTGTTCAG TACAAGGTGC GCATTGATTT TATTGTGCAG CGTGAAATAG TCCATGGCCT 
0361 TAAGTATGTT CAAAAGACTT CTCGCTTGGG TGTTAATGTT GACAAGATGA AGCATATGGT 
0421 TGGCTCCTAT CCGCCGAAGA AGGAGATTCA GTTCTATTTG ACGCCCGCCG AGGAGGCACC 
0481 TTCAGGCACA TT-CTCCCGC G

Assemble Data


7823R-4.IR_full       1   AGAGACGAAGCACCATCCCGAGCACCACGACGACGATGTCCACGATGCCAACTACCAGGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7823R-4.in silico     1   AGAGACGAAGCACCATCCCGAGCACCACGACGACGATGTCCACGATGCCAACTACCAGGC 60


7823R-4.IR_full       61  GCCGCCGGAGAAGACCATCGAGGAAATAATGGCCGCCGATCAGGAGGATGAGAGCTTGCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7823R-4.in silico     61  GCCGCCGGAGAAGACCATCGAGGAAATAATGGCCGCCGATCAGGAGGATGAGAGCTTGCG 120


7823R-4.IR_full       121 GCGCTACAAGGAGGCGCTCCTGGGTGCCGCCCAGACCGAGAAAATTATTGTTGAACCCAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7823R-4.in silico     121 GCGCTACAAGGAGGCGCTCCTGGGTGCCGCCCAGACCGAGAAAATTATTGTTGAACCCAA 180


7823R-4.IR_full       181 TGATCCCCGAAAAGTTATTGTAAAGAAACTGGCCCTCGTTGTCGAAGGACGCGACGACAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7823R-4.in silico     181 TGATCCCCGAAAAGTTATTGTAAAGAAACTGGCCCTCGTTGTCGAAGGACGCGACGACAT 240


7823R-4.IR_full       241 GGAATTGGATCTTACCGGTGACCTTAGTCAGCTGAAAAAGCAGCTTTTTGTGATCAAAGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7823R-4.in silico     241 GGAATTGGATCTTACCGGTGACCTTAGTCAGCTGAAAAAGCAGCTTTTTGTGATCAAAGA 300


7823R-4.IR_full       301 AGGTGTTCAGTACAAGGTGCGCATTGATTTTATTGTGCAGCGTGAAATAGTCCATGGCCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7823R-4.in silico     301 AGGTGTTCAGTACAAGGTGCGCATTGATTTTATTGTGCAGCGTGAAATAGTCCATGGCCT 360


7823R-4.IR_full       361 TAAGTATGTTCAAAAGACTTCTCGCTTGGGTGTTAATGTTGACAAGATGAAGCATATGGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7823R-4.in silico     361 TAAGTATGTTCAAAAGACTTCTCGCTTGGGTGTTAATGTTGACAAGATGAAGCATATGGT 420


7823R-4.IR_full       421 TGGCTCCTATCCGCCGAAGAAGGAGATTCAGTTCTATTTGACGCCCGCCGAGGAGGCACC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7823R-4.in silico     421 TGGCTCCTATCCGCCGAAGAAGGAGATTCAGTTCTATTTGACGCCCGCCGAGGAGGCACC 480


7823R-4.IR_full       481 TTCAGGCACATTNCNCCCGCG 501
                          |||||||||||| | ||||||
7823R-4.in silico     481 TTCAGGCACATT-CTCCCGCG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140905.2	CG7823-RA (RhoGDI), mRNA
0	0	5	8	20	NM_130678.2	CG14419-RA (CG14419), mRNA
0	0	2	3	4	NM_167538.1	CG32572-RA (CG32572), mRNA
0	0	2	2	2	NM_164965.1	CG4881-RB, transcript variant B (salr), mRNA
0	0	2	2	2	NM_078824.2	CG4881-RA, transcript variant A (salr), mRNA
0	0	2	2	2	NM_140171.2	CG32075-RA (CG32075), mRNA
0	0	1	11	9	NM_079845.2	CG7951-RA (sima), mRNA
0	0	1	8	2	NM_168574.1	CG6603-RC, transcript variant C (Hsc70Cb), mRNA
0	0	1	8	2	NM_168573.1	CG6603-RB, transcript variant B (Hsc70Cb), mRNA
0	0	1	8	2	NM_140430.2	CG6603-RA, transcript variant A (Hsc70Cb), mRNA
0	0	1	5	5	NM_167278.1	CG11759-RA (Kap3), mRNA
0	0	1	4	7	NM_132059.2	CG4766-RA (CG4766), mRNA
0	0	1	4	3	NM_167313.1	CG2467-RB, transcript variant B (CG2467), mRNA
0	0	1	4	3	NM_132540.1	CG2467-RA, transcript variant A (CG2467), mRNA
0	0	1	3	9	NM_176374.1	CG4761-RA (knrl), mRNA
0	0	1	3	3	NM_079768.2	CG6238-RA, transcript variant A (ssh), mRNA
0	0	1	3	3	NM_176247.1	CG33143-RB, transcript variant B (CG33143), mRNA
0	0	1	3	2	NM_132904.1	CG9903-RA (CG9903), mRNA
0	0	1	2	7	NM_142984.1	CG6364-RA (CG6364), mRNA
0	0	1	2	5	NM_165550.1	CG30493-RB (CG30493), mRNA
0	0	1	2	3	NM_139836.2	CG8610-RA (Cdc27), mRNA
0	0	1	2	3	NM_138988.2	CG11405-RA (A3-3), mRNA
0	0	1	2	2	NM_001042833.1	CG41480-RA (CG41480), mRNA
0	0	1	2	2	NM_001015346.1	CG40084-PC.3 (CG40084), mRNA
0	0	1	2	2	NM_001015347.1	CG40084-PD.3 (CG40084), mRNA
0	0	0	6	8	NM_079205.3	CG7507-RA, transcript variant A (Dhc64C), mRNA
0	0	0	6	8	NM_168103.1	CG7507-RB, transcript variant B (Dhc64C), mRNA
0	0	0	6	6	NM_167570.1	CG8465-RC, transcript variant C (l(1)G0222), mRNA
0	0	0	6	6	NM_132993.2	CG8465-RA, transcript variant A (l(1)G0222), mRNA
0	0	0	6	6	NM_167569.1	CG8465-RB, transcript variant B (l(1)G0222), mRNA

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