NIG-Fly - Fly Stocks of National Institute of Genetics -

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7770R-3

Symbol CG7770 Full Name CG7770

CG No CG7770 Old CG No CG7770

Synonyms BcDNA:GH28557, CG7770

Accession No (Link to NCBI) NM_140902.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGACAAGAAGAGCGCAGCGT

Primer Seq. 3'

aatctagaggtaccCCGCCACCTGACACTGCTGTT

Predicted Fragment Size

IR fragment full Seq

0001 TGGACAAGAA GAGCGCNGcN TNGTACAAAA AGATGCAGGC CGAGATTGAG TCGTACCAGA 
0061 ACCTGCAAAA ATCCTGCTTG AAGATGGTGA AGCAGCGAGC AGTGCTCGAA AGCCAGCTGA 
0121 ACGAGAACAA GTGCGTCCTG GACGAGCTGA ATCTTCTGGG CCCCGACAAC AAGGTCTACA 
0181 AGCTCTTCGG GCCCGTTCTG GTCAAACAGG AGCTGGAGGA GTCGCGCCAG AATGTCGGAA 
0241 AGCGCATCGA GTACATCTCC AAGGAGCTGA AGAGCTCCAC CGACGCACTG GAGAACATGG 
0301 AGAAGGACAT GCTGAAGCAT CGGGAATCCG TGGCCAAGTA CCAACAGCAG TGTCAGGTGG 
0361 CGG

in silico PCR Fragment

0001 TGGACAAGAA GAGCGCAGCG TTGTACAAAA AGATGCAGGC CGAGATTGAG TCGTACCAGA 
0061 ACCTGCAAAA ATCCTGCTTG AAGATGGTGA AGCAGCGAGC AGTGCTCGAA AGCCAGCTGA 
0121 ACGAGAACAA GTGCGTCCTG GACGAGCTGA ATCTTCTGGG CCCCGACAAC AAGGTCTACA 
0181 AGCTCTTCGG GCCCGTTCTG GTCAAACAGG AGCTGGAGGA GTCGCGCCAG AATGTCGGAA 
0241 AGCGCATCGA GTACATCTCC AAGGAGCTGA AGAGCTCCAC CGACGCACTG GAGAACATGG 
0301 AGAAGGACAT GCTGAAGCAT CGGGAATCCG TGGCCAAGTA CCAACAGCAG TGTCAGGTGG 
0361 CGG

Assemble Data


7770R-3.IR_full       1   TGGACAAGAAGAGCGCNGCNTNGTACAAAAAGATGCAGGCCGAGATTGAGTCGTACCAGA 60
                          |||||||||||||||| || | ||||||||||||||||||||||||||||||||||||||
7770R-3.in silico     1   TGGACAAGAAGAGCGCAGCGTTGTACAAAAAGATGCAGGCCGAGATTGAGTCGTACCAGA 60


7770R-3.IR_full       61  ACCTGCAAAAATCCTGCTTGAAGATGGTGAAGCAGCGAGCAGTGCTCGAAAGCCAGCTGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7770R-3.in silico     61  ACCTGCAAAAATCCTGCTTGAAGATGGTGAAGCAGCGAGCAGTGCTCGAAAGCCAGCTGA 120


7770R-3.IR_full       121 ACGAGAACAAGTGCGTCCTGGACGAGCTGAATCTTCTGGGCCCCGACAACAAGGTCTACA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7770R-3.in silico     121 ACGAGAACAAGTGCGTCCTGGACGAGCTGAATCTTCTGGGCCCCGACAACAAGGTCTACA 180


7770R-3.IR_full       181 AGCTCTTCGGGCCCGTTCTGGTCAAACAGGAGCTGGAGGAGTCGCGCCAGAATGTCGGAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7770R-3.in silico     181 AGCTCTTCGGGCCCGTTCTGGTCAAACAGGAGCTGGAGGAGTCGCGCCAGAATGTCGGAA 240


7770R-3.IR_full       241 AGCGCATCGAGTACATCTCCAAGGAGCTGAAGAGCTCCACCGACGCACTGGAGAACATGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7770R-3.in silico     241 AGCGCATCGAGTACATCTCCAAGGAGCTGAAGAGCTCCACCGACGCACTGGAGAACATGG 300


7770R-3.IR_full       301 AGAAGGACATGCTGAAGCATCGGGAATCCGTGGCCAAGTACCAACAGCAGTGTCAGGTGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7770R-3.in silico     301 AGAAGGACATGCTGAAGCATCGGGAATCCGTGGCCAAGTACCAACAGCAGTGTCAGGTGG 360


7770R-3.IR_full       361 CGG 363
                          |||
7770R-3.in silico     361 CGG 363

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	345	0	0	0	NM_140902.1	CG7770-RA (CG7770), mRNA
0	0	6	4	5	NM_142949.3	CG5977-RB, transcript variant B (spas), mRNA
0	0	6	4	5	NM_170115.2	CG5977-RA, transcript variant A (spas), mRNA
0	0	4	2	5	NM_176177.1	CG33139-RA (Ranbp11), mRNA
0	0	2	5	3	NM_170553.1	CG1528-RB, transcript variant B (gammaCop), mRNA
0	0	2	5	3	NM_079869.2	CG1528-RA, transcript variant A (gammaCop), mRNA
0	0	2	3	9	NM_057830.3	CG9433-RB, transcript variant B (Xpd), mRNA
0	0	2	3	9	NM_166429.1	CG9433-RA, transcript variant A (Xpd), mRNA
0	0	2	3	8	NM_166040.1	CG8479-RA, transcript variant A (CG8479), mRNA
0	0	2	3	8	NM_137097.2	CG8479-RB, transcript variant B (CG8479), mRNA
0	0	2	2	3	NM_078656.3	CG9108-RA (RSG7), mRNA
0	0	1	4	8	NM_135436.1	CG17005-RA (CG17005), mRNA
0	0	1	4	4	NM_138258.2	CG2211-RA (CG2211), mRNA
0	0	1	4	3	NM_166623.1	CG4051-RA (egl), mRNA
0	0	1	3	4	NM_134997.2	CG17840-RA (CG17840), mRNA
0	0	1	3	4	NM_140733.2	CG6322-RA (CG6322), mRNA
0	0	1	2	6	NM_001031867.1	CG33950-RF, transcript variant F (trol), mRNA
0	0	1	2	5	NM_134544.1	CG11734-RB (HERC2), mRNA
0	0	1	2	4	NM_137195.2	CG8180-RA (CG8180), mRNA
0	0	1	2	3	NM_140955.2	CG5649-RA (kin17), mRNA
0	0	1	2	3	NM_170139.1	CG31132-RA (BRWD3), mRNA
0	0	1	2	2	NM_167408.1	CG32600-RA (dpr8), mRNA
0	0	0	8	3	NM_143086.2	CG13651-RA (danr), mRNA
0	0	0	5	6	NM_165597.1	CG8705-RB, transcript variant B (pnut), mRNA
0	0	0	5	6	NM_057716.3	CG8705-RA, transcript variant A (pnut), mRNA
0	0	0	5	6	NM_176427.1	CG9755-RE, transcript variant E (pum), mRNA
0	0	0	5	5	NM_169258.1	CG9755-RA, transcript variant A (pum), mRNA
0	0	0	5	5	NM_163888.1	CG32491-RK, transcript variant K (mod(mdg4)), mRNA
0	0	0	5	5	NM_169260.1	CG9755-RB, transcript variant B (pum), mRNA
0	0	0	5	5	NM_079561.2	CG9755-RC, transcript variant C (pum), mRNA

Back