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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7764R-2 |
| Symbol |
Tfb2 |
Full Name |
Tfb2 |
| CG No |
CG7764 |
Old CG No |
CG7764 |
| Synonyms |
154538_at, TFB2, CG7764, p52, anon-WO0118547.648, Tfb2 |
| Accession No (Link to NCBI) |
NM_140523.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
2 lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.<br> A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.<br> Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGCCGACACGAAATCCGGT |
| Primer Seq. 3' |
aatctagaggtaccCAATGTCCCTGGGCTTCGAGT |
| Predicted Fragment Size |
490 |
| IR fragment full Seq |
0001 ATGGCCGACA CGAAATCCGG TAGTGGTCGG CATTCGGCTG CCGGGGGAAG TGGAAACGCC
0061 GTCCTGTCGC CCTTGGGGCA GGGTCCTGCG AACTTGGAGT GCAAGGACTT TCAGGAGTAT
0121 CTGCGCACCC GCCAAACGCC AGAGTCGCTG GAGAAGCTGT ACAACTATCC GCCCATTTGT
0181 TTGGCCGTTT TTCGGGAGCT TCCAGAGATA GCCCGGCAGT TTATCATAAG GATACTGTTC
0241 GTGGATCAGC CTGTGCCACA AGCGGTGGTC ACATCATGGG GTGCCCAACG TTGTGCCAAG
0301 GAGCAAGCCG AGGCAACCAG CTGCCTGACG GCTTTGAACG TCTGGCGGGT CACAGCCATT
0361 CCGGGTGGCC TTTCTGCGTG GGAGCTCTCG CCCACCTTCA AGAAAAGCGT ACGCCAGGTG
0421 CTGCTGGGCG GCGGAAAACC TTGGCCTATG ACCAACACGC TGGATAAGGA CTCGAAGCCC
0481 AGGGACATTG |
| in silico PCR Fragment |
0001 ATGGCCGACA CGAAATCCGG TAGTGGTCGG CATTCGGCTG CCGGGGGAAG TGGAAACGCC
0061 GTCCTGTCGC CCTTGGGGCA GGGTCCTGCG AACTTGGAGT GTAAGGACTT TCAGGAGTAT
0121 CTGCGCACCC GCCAAACGCC AGAGTCGCTG GAGAAGCTGT ACAACTATCC GCCCATTTGT
0181 TTGGCCGTTT TTCGGGAGCT TCCAGAGATA GCCCGGCAGT TTATCATAAG GATACTGTTC
0241 GTGGATCAGC CTGTGCCACA AGCGGTGGTC ACATCATGGG GTGCCCAACG TTGTGCCAAG
0301 GAGCAAGCCG AGGCAACCAG CTGCCTGACG GCTTTGAACG TCTGGCGGGT CACAGCCATT
0361 CCGGGTGGCC TTTCTGCGTG GGAGCTCTCG CCCACCTTCA AGAAAAGCGT ACGCCAGGTG
0421 CTGCTGGGCG GCGGAAAACC TTGGCCTATG ACCAACACGC TGGATAAGGA CTCGAAGCCC
0481 AGGGACATTG |
| Assemble Data |
<PRE>
7764R-2.IR_full 1 ATGGCCGACACGAAATCCGGTAGTGGTCGGCATTCGGCTGCCGGGGGAAGTGGAAACGCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7764R-2.in silico 1 ATGGCCGACACGAAATCCGGTAGTGGTCGGCATTCGGCTGCCGGGGGAAGTGGAAACGCC 60
7764R-2.IR_full 61 GTCCTGTCGCCCTTGGGGCAGGGTCCTGCGAACTTGGAGTG<span class="snp"><tt>C</tt></span>AAGGACTTTCAGGAGTAT 120
|||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||
7764R-2.in silico 61 GTCCTGTCGCCCTTGGGGCAGGGTCCTGCGAACTTGGAGTG<span class="snp"><tt>T</tt></span>AAGGACTTTCAGGAGTAT 120
7764R-2.IR_full 121 CTGCGCACCCGCCAAACGCCAGAGTCGCTGGAGAAGCTGTACAACTATCCGCCCATTTGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7764R-2.in silico 121 CTGCGCACCCGCCAAACGCCAGAGTCGCTGGAGAAGCTGTACAACTATCCGCCCATTTGT 180
7764R-2.IR_full 181 TTGGCCGTTTTTCGGGAGCTTCCAGAGATAGCCCGGCAGTTTATCATAAGGATACTGTTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7764R-2.in silico 181 TTGGCCGTTTTTCGGGAGCTTCCAGAGATAGCCCGGCAGTTTATCATAAGGATACTGTTC 240
7764R-2.IR_full 241 GTGGATCAGCCTGTGCCACAAGCGGTGGTCACATCATGGGGTGCCCAACGTTGTGCCAAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7764R-2.in silico 241 GTGGATCAGCCTGTGCCACAAGCGGTGGTCACATCATGGGGTGCCCAACGTTGTGCCAAG 300
7764R-2.IR_full 301 GAGCAAGCCGAGGCAACCAGCTGCCTGACGGCTTTGAACGTCTGGCGGGTCACAGCCATT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7764R-2.in silico 301 GAGCAAGCCGAGGCAACCAGCTGCCTGACGGCTTTGAACGTCTGGCGGGTCACAGCCATT 360
7764R-2.IR_full 361 CCGGGTGGCCTTTCTGCGTGGGAGCTCTCGCCCACCTTCAAGAAAAGCGTACGCCAGGTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7764R-2.in silico 361 CCGGGTGGCCTTTCTGCGTGGGAGCTCTCGCCCACCTTCAAGAAAAGCGTACGCCAGGTG 420
7764R-2.IR_full 421 CTGCTGGGCGGCGGAAAACCTTGGCCTATGACCAACACGCTGGATAAGGACTCGAAGCCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7764R-2.in silico 421 CTGCTGGGCGGCGGAAAACCTTGGCCTATGACCAACACGCTGGATAAGGACTCGAAGCCC 480
7764R-2.IR_full 481 AGGGACATTG 490
||||||||||
7764R-2.in silico 481 AGGGACATTG 490
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
472 |
0 |
0 |
0 |
NM_140523.2 |
CG7764-RA (Tfb2), mRNA |
| 0 |
0 |
4 |
3 |
7 |
NM_168037.1 |
CG32264-RA, transcript variant A (CG32264), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_168038.1 |
CG32264-RD, transcript variant D (CG32264), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_206268.1 |
CG32264-RC, transcript variant C (CG32264), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_057309.2 |
CG6534-RA (slou), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_132740.1 |
CG15890-RA (CG15890), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079959.2 |
CG4501-RA (bgm), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_078937.3 |
CG2411-RA (ptc), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080189.2 |
CG16880-RA (l(2)34Fa), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_205987.1 |
CG32972-RA, transcript variant A (CG32972), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_176037.1 |
CG32972-RB, transcript variant B (CG32972), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137809.1 |
CG3292-RA (CG3292), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166223.1 |
CG6556-RA (cnk), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_131948.1 |
CG3081-RA (CG3081), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_079149.3 |
CG17046-RA, transcript variant A (klar), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_001043111.1 |
CG17046-RB, transcript variant B (klar), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137431.2 |
CG14492-RA (CG14492), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136792.1 |
CG13232-RA (BBS4), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_078853.2 |
CG4767-RA (Tektin-A), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_166076.1 |
CG12424-RB, transcript variant B (CG12424), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_137165.1 |
CG12424-RA, transcript variant A (CG12424), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_166077.1 |
CG12424-RC, transcript variant C (CG12424), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_139356.1 |
CG3524-RA (v(2)k05816), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_136563.1 |
CG14746-RA (PGRP-SC1a), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_136565.1 |
CG8577-RA (PGRP-SC1b), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_132126.1 |
CG3075-RA (CG3075), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_142003.1 |
CG8138-RA (CG8138), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_132673.2 |
CG11178-RB, transcript variant B (CG11178), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_167377.1 |
CG11178-RA, transcript variant A (CG11178), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_168412.1 |
CG32062-RD, transcript variant D (CG32062), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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