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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7739R-3

Symbol

CG7739

Full Name

CG7739

CG No

CG7739

Old CG No

CG7739

Synonyms

CG7739

Accession No (Link to NCBI)

NM_140519.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTGTATTGGGTCTCCTGCTGG

Primer Seq. 3'

aatctagaggtaccCGGAAGCCTACTGCTGTTGAA

Predicted Fragment Size

IR fragment full Seq

0001 TTGTATTGGG TCTCCTGCTG GCCCTGGGCA ACCTGATTCA CGCCAGTAAT ATCACTGATA 
0061 AAGTTTTTGC AGACATTAAA GAGGGCATTG TGGCAGCTTT CGGAGATTTC AATTCCGACG 
0121 AACTCACGGA TGTTTTTGTA ATTCGCGATG GGATGAAGAG GTTGCAGATA CTCTTCGGGC 
0181 ACTATACGGA GCCTCTGCTT AAACTGGGAC CCGACTGCCA GTACGGAGAT AAGACCATTG 
0241 TTAGTGTTAT ACCTGGAGAT TTTGATGGCG ACGCCCTGAT GGACGTGCTG GTAAACCTAA 
0301 AGGGGAAGGA CGAGGAGACC TACGAGGTGC ACATCAACTG GGGCAATACC ACCGAACTGA 
0361 AGTGCTCCAA TAAGTCACTT TTTACCAGTA AAGGAGAGGT AATGGCCCTG GACTTTAACC 
0421 GCGATATGAT TATCGATTTA TACGGACTTG ATTCTGCGGA AAGGCGTACA TTCTGGATAT 
0481 TCAACAGCAG TAGGCTTCCG

in silico PCR Fragment

0001 TTGTATTGGG TCTCCTGCTG GCCCTGGGCA ACCTGATTCA CGCCAGTAAT ATCACTGATA 
0061 AAGTTTTTGC AGACATTAAA GAGGGCATTG TGGCAGCTTT CGGAGATTTC AATTCCGACG 
0121 AACTCACGGA TGTTTTTGTA ATTCGCGATG GGATGAAGAG GTTGCAGATA CTCTTCGGGC 
0181 ACTATACGGA GCCTCTGCTT AAACTGGGAC CCGACTGCCA GTACGGAGAT AAGACCATTG 
0241 TTAGTGTTAT ACCTGGAGAT TTTGATGGCG ACGCCCTGAT GGACGTGCTG GTAAACCTGA 
0301 AGGGGAAGGA CGAGGAGACC TACGAGGTGC ACATCAACTG GGGCAATACC ACCGAACTGA 
0361 AGTGCTCCAA TAAGTCACTT TTTACCAGTA AAGGAGAGGT AATGGCCCTG GACTTTAACC 
0421 GCGATATGAT TATCGATTTA TACGGACTTG ATTCTGCGGA AAGGCGTACA TTCTGGATAT 
0481 TCAACAGCAG TAGGCTTCCG

Assemble Data

<PRE>

7739R-3.IR_full       1   TTGTATTGGGTCTCCTGCTGGCCCTGGGCAACCTGATTCACGCCAGTAATATCACTGATA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-3.in silico     1   TTGTATTGGGTCTCCTGCTGGCCCTGGGCAACCTGATTCACGCCAGTAATATCACTGATA 60


7739R-3.IR_full       61  AAGTTTTTGCAGACATTAAAGAGGGCATTGTGGCAGCTTTCGGAGATTTCAATTCCGACG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-3.in silico     61  AAGTTTTTGCAGACATTAAAGAGGGCATTGTGGCAGCTTTCGGAGATTTCAATTCCGACG 120


7739R-3.IR_full       121 AACTCACGGATGTTTTTGTAATTCGCGATGGGATGAAGAGGTTGCAGATACTCTTCGGGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-3.in silico     121 AACTCACGGATGTTTTTGTAATTCGCGATGGGATGAAGAGGTTGCAGATACTCTTCGGGC 180


7739R-3.IR_full       181 ACTATACGGAGCCTCTGCTTAAACTGGGACCCGACTGCCAGTACGGAGATAAGACCATTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-3.in silico     181 ACTATACGGAGCCTCTGCTTAAACTGGGACCCGACTGCCAGTACGGAGATAAGACCATTG 240


7739R-3.IR_full       241 TTAGTGTTATACCTGGAGATTTTGATGGCGACGCCCTGATGGACGTGCTGGTAAACCT<span class="snp"><tt>A</tt></span>A 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
7739R-3.in silico     241 TTAGTGTTATACCTGGAGATTTTGATGGCGACGCCCTGATGGACGTGCTGGTAAACCT<span class="snp"><tt>G</tt></span>A 300


7739R-3.IR_full       301 AGGGGAAGGACGAGGAGACCTACGAGGTGCACATCAACTGGGGCAATACCACCGAACTGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-3.in silico     301 AGGGGAAGGACGAGGAGACCTACGAGGTGCACATCAACTGGGGCAATACCACCGAACTGA 360


7739R-3.IR_full       361 AGTGCTCCAATAAGTCACTTTTTACCAGTAAAGGAGAGGTAATGGCCCTGGACTTTAACC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-3.in silico     361 AGTGCTCCAATAAGTCACTTTTTACCAGTAAAGGAGAGGTAATGGCCCTGGACTTTAACC 420


7739R-3.IR_full       421 GCGATATGATTATCGATTTATACGGACTTGATTCTGCGGAAAGGCGTACATTCTGGATAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-3.in silico     421 GCGATATGATTATCGATTTATACGGACTTGATTCTGCGGAAAGGCGTACATTCTGGATAT 480


7739R-3.IR_full       481 TCAACAGCAGTAGGCTTCCG 500
                          ||||||||||||||||||||
7739R-3.in silico     481 TCAACAGCAGTAGGCTTCCG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140519.2	CG7739-RA (CG7739), mRNA
0	0	8	3	6	NM_170234.1	CG17383-RC, transcript variant C (CG17383), mRNA
0	0	8	3	6	NM_170235.1	CG17383-RD, transcript variant D (CG17383), mRNA
0	0	8	3	6	NM_170233.1	CG17383-RA, transcript variant A (CG17383), mRNA
0	0	8	3	6	NM_143152.2	CG17383-RB, transcript variant B (CG17383), mRNA
0	0	3	5	4	NM_164705.1	CG11325-RB, transcript variant B (GRHR), mRNA
0	0	3	5	4	NM_205917.1	CG11325-RC, transcript variant C (GRHR), mRNA
0	0	3	5	4	NM_058039.2	CG11325-RA, transcript variant A (GRHR), mRNA
0	0	3	3	4	NM_176149.1	CG33145-RA, transcript variant A (CG33145), mRNA
0	0	3	3	4	NM_176148.1	CG33145-RB, transcript variant B (CG33145), mRNA
0	0	2	5	7	NM_080314.2	CG8590-RA (Klp3A), mRNA
0	0	2	3	3	NM_080358.2	CG1484-RA (fliI), mRNA
0	0	2	3	3	NM_136986.2	CG3884-RB, transcript variant B (CG3884), mRNA
0	0	2	3	2	NM_206045.1	CG3572-RC, transcript variant C (vimar), mRNA
0	0	2	3	2	NM_057957.4	CG3572-RB, transcript variant B (vimar), mRNA
0	0	2	2	4	NM_001014684.1	CG7921-RB, transcript variant B (Mgat2), mRNA
0	0	2	2	4	NM_143506.3	CG7921-RA, transcript variant A (Mgat2), mRNA
0	0	1	5	2	NM_143059.1	CG13646-RA (CG13646), mRNA
0	0	1	3	7	NM_170107.2	CG31184-RA (CG31184), mRNA
0	0	1	3	6	NM_001043070.1	CG34146-RA (brp), mRNA
0	0	1	2	3	NM_080142.2	CG1925-RA (mus205), mRNA
0	0	1	2	2	NM_080084.2	CG8428-RD, transcript variant D (spin), mRNA
0	0	1	2	2	NM_166144.1	CG8428-RC, transcript variant C (spin), mRNA
0	0	1	2	2	NM_166145.1	CG8428-RA, transcript variant A (spin), mRNA
0	0	1	2	2	NM_166146.1	CG8428-RB, transcript variant B (spin), mRNA
0	0	1	2	2	NM_166147.1	CG8428-RE, transcript variant E (spin), mRNA
0	0	0	6	5	NM_170412.1	CG2010-RA, transcript variant A (CG2010), mRNA
0	0	0	6	5	NM_143439.2	CG2010-RB, transcript variant B (CG2010), mRNA
0	0	0	5	5	NM_078605.1	CG6211-RA (gce), mRNA
0	0	0	4	9	NM_001032076.1	CG33694-RA, transcript variant A (cana), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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