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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7739R-1 |
| Symbol |
CG7739 |
Full Name |
CG7739 |
| CG No |
CG7739 |
Old CG No |
CG7739 |
| Synonyms |
CG7739 |
| Accession No (Link to NCBI) |
NM_140519.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTGTATTGGGTCTCCTGCTGG |
| Primer Seq. 3' |
aatctagaggtaccCGGAAGCCTACTGCTGTTGAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTGTATTGGG TCTCCTGCTG GCCCTGGGCA ACCTGATTCA CGCCAGTAAT ATCACTGATA
0061 AAGTTTTTGC AGACATTAAA GAGGGCATTG TGGCAGCTTT CGGAGATTTC AATTCCGACG
0121 AACTCACGGA TGTTTTTGTA ATTCGCGATG GGATGAAGAG GTTGCAGATA CTCTTCGGGC
0181 ACTATACGGA GCCTCTGCTT AAACTGGGAC CCGACTGCCA GTACGGAGAT AAGACCATTG
0241 TTAGTGTTAT ACCTGGAGAT TTTGATGGCG ACGCCCTGAT GGACGTGCTG GTAAACCTAA
0301 AGGGGAAGGA CGAGGAGACC TACGAGGTGC ACATCAACTG GGGCAATACC ACCGAACTGA
0361 AGTGCTCCAA TAAGTCACTT TTTACCAGTA AAGGAGAGGT AATGGCCCTG GACTTTAACC
0421 GCGATATGAT TATCGATTTA TACGGACTTG ATTCTGCGGA AAGGCGTACA TTCTGGATAT
0481 TCAACAGCAG TAGGCTTCCG |
| in silico PCR Fragment |
0001 TTGTATTGGG TCTCCTGCTG GCCCTGGGCA ACCTGATTCA CGCCAGTAAT ATCACTGATA
0061 AAGTTTTTGC AGACATTAAA GAGGGCATTG TGGCAGCTTT CGGAGATTTC AATTCCGACG
0121 AACTCACGGA TGTTTTTGTA ATTCGCGATG GGATGAAGAG GTTGCAGATA CTCTTCGGGC
0181 ACTATACGGA GCCTCTGCTT AAACTGGGAC CCGACTGCCA GTACGGAGAT AAGACCATTG
0241 TTAGTGTTAT ACCTGGAGAT TTTGATGGCG ACGCCCTGAT GGACGTGCTG GTAAACCTGA
0301 AGGGGAAGGA CGAGGAGACC TACGAGGTGC ACATCAACTG GGGCAATACC ACCGAACTGA
0361 AGTGCTCCAA TAAGTCACTT TTTACCAGTA AAGGAGAGGT AATGGCCCTG GACTTTAACC
0421 GCGATATGAT TATCGATTTA TACGGACTTG ATTCTGCGGA AAGGCGTACA TTCTGGATAT
0481 TCAACAGCAG TAGGCTTCCG |
| Assemble Data |
<PRE>
7739R-1.IR_full 1 TTGTATTGGGTCTCCTGCTGGCCCTGGGCAACCTGATTCACGCCAGTAATATCACTGATA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-1.in silico 1 TTGTATTGGGTCTCCTGCTGGCCCTGGGCAACCTGATTCACGCCAGTAATATCACTGATA 60
7739R-1.IR_full 61 AAGTTTTTGCAGACATTAAAGAGGGCATTGTGGCAGCTTTCGGAGATTTCAATTCCGACG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-1.in silico 61 AAGTTTTTGCAGACATTAAAGAGGGCATTGTGGCAGCTTTCGGAGATTTCAATTCCGACG 120
7739R-1.IR_full 121 AACTCACGGATGTTTTTGTAATTCGCGATGGGATGAAGAGGTTGCAGATACTCTTCGGGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-1.in silico 121 AACTCACGGATGTTTTTGTAATTCGCGATGGGATGAAGAGGTTGCAGATACTCTTCGGGC 180
7739R-1.IR_full 181 ACTATACGGAGCCTCTGCTTAAACTGGGACCCGACTGCCAGTACGGAGATAAGACCATTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-1.in silico 181 ACTATACGGAGCCTCTGCTTAAACTGGGACCCGACTGCCAGTACGGAGATAAGACCATTG 240
7739R-1.IR_full 241 TTAGTGTTATACCTGGAGATTTTGATGGCGACGCCCTGATGGACGTGCTGGTAAACCT<span class="snp"><tt>A</tt></span>A 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|
7739R-1.in silico 241 TTAGTGTTATACCTGGAGATTTTGATGGCGACGCCCTGATGGACGTGCTGGTAAACCT<span class="snp"><tt>G</tt></span>A 300
7739R-1.IR_full 301 AGGGGAAGGACGAGGAGACCTACGAGGTGCACATCAACTGGGGCAATACCACCGAACTGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-1.in silico 301 AGGGGAAGGACGAGGAGACCTACGAGGTGCACATCAACTGGGGCAATACCACCGAACTGA 360
7739R-1.IR_full 361 AGTGCTCCAATAAGTCACTTTTTACCAGTAAAGGAGAGGTAATGGCCCTGGACTTTAACC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-1.in silico 361 AGTGCTCCAATAAGTCACTTTTTACCAGTAAAGGAGAGGTAATGGCCCTGGACTTTAACC 420
7739R-1.IR_full 421 GCGATATGATTATCGATTTATACGGACTTGATTCTGCGGAAAGGCGTACATTCTGGATAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7739R-1.in silico 421 GCGATATGATTATCGATTTATACGGACTTGATTCTGCGGAAAGGCGTACATTCTGGATAT 480
7739R-1.IR_full 481 TCAACAGCAGTAGGCTTCCG 500
||||||||||||||||||||
7739R-1.in silico 481 TCAACAGCAGTAGGCTTCCG 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140519.2 |
CG7739-RA (CG7739), mRNA |
| 0 |
0 |
8 |
3 |
6 |
NM_170234.1 |
CG17383-RC, transcript variant C (CG17383), mRNA |
| 0 |
0 |
8 |
3 |
6 |
NM_170235.1 |
CG17383-RD, transcript variant D (CG17383), mRNA |
| 0 |
0 |
8 |
3 |
6 |
NM_170233.1 |
CG17383-RA, transcript variant A (CG17383), mRNA |
| 0 |
0 |
8 |
3 |
6 |
NM_143152.2 |
CG17383-RB, transcript variant B (CG17383), mRNA |
| 0 |
0 |
3 |
5 |
4 |
NM_164705.1 |
CG11325-RB, transcript variant B (GRHR), mRNA |
| 0 |
0 |
3 |
5 |
4 |
NM_205917.1 |
CG11325-RC, transcript variant C (GRHR), mRNA |
| 0 |
0 |
3 |
5 |
4 |
NM_058039.2 |
CG11325-RA, transcript variant A (GRHR), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_176149.1 |
CG33145-RA, transcript variant A (CG33145), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_176148.1 |
CG33145-RB, transcript variant B (CG33145), mRNA |
| 0 |
0 |
2 |
5 |
7 |
NM_080314.2 |
CG8590-RA (Klp3A), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_080358.2 |
CG1484-RA (fliI), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_136986.2 |
CG3884-RB, transcript variant B (CG3884), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206045.1 |
CG3572-RC, transcript variant C (vimar), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_057957.4 |
CG3572-RB, transcript variant B (vimar), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001014684.1 |
CG7921-RB, transcript variant B (Mgat2), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_143506.3 |
CG7921-RA, transcript variant A (Mgat2), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_143059.1 |
CG13646-RA (CG13646), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_170107.2 |
CG31184-RA (CG31184), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_001043070.1 |
CG34146-RA (brp), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080142.2 |
CG1925-RA (mus205), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080084.2 |
CG8428-RD, transcript variant D (spin), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166144.1 |
CG8428-RC, transcript variant C (spin), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166145.1 |
CG8428-RA, transcript variant A (spin), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166146.1 |
CG8428-RB, transcript variant B (spin), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166147.1 |
CG8428-RE, transcript variant E (spin), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_170412.1 |
CG2010-RA, transcript variant A (CG2010), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_143439.2 |
CG2010-RB, transcript variant B (CG2010), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_078605.1 |
CG6211-RA (gce), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_001032076.1 |
CG33694-RA, transcript variant A (cana), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| + |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
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|
