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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7716R-3 
 Symbol CG7716  Full Name CG7716 
 CG No CG7716  Old CG No CG7716 
 Synonyms CG7716 
 Accession No (Link to NCBI) NM_139882.1 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGTGACTTCGCGAACAAAGTG 
 Primer Seq. 3' 
aatctagaggtaccCTTCCGCAGTTAGGCGACATA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGTGACTTCG CGAACAAAGT GCGCTTTCAC CTCAGTAAGG ACGAGCTGGA GCGGCAGATG 
0061 AGGAAAATTC ACGACCGTGA GGAATCCGTA GACGAAAAAA TGCGTCCATC AGGAAGCGAA 
0121 AACAGCGGTC ATAATTGCAA TACGAGCTCG TCCTCAAGTC TGCCACGGCA GGAGTCCTGG 
0181 ATCTTCGAGA GCGTTGGGGG TTACTTTCTC GCCAACGAAA ATTTGGCCAA GATGAGTTTG 
0241 CCTTGTCAGG AACAGTTGCT GATGAGGGAT CTAATCTATG CCTTCTCGGG TGTTCCATCT 
0301 GCATATGTAA AGCCTGATAT TCAGATCGAT CAGATTTCCG AAATGTCTGC AGTGGATATA 
0361 GCCAAGATTC GCTTTCGATT GAAAAAGACC TTCACTGGGG CTTTTAGGGC TTTGGCCAAT 
0421 GAAACATTAC CCCTGATAGG TTATTATATT AGTGTACAGA GCTATATAGA GGAGACCAAT 
0481 ATGTCGCCTA ACTGcGGAAG  
 in silico PCR Fragment 
0001 AGTGACTTCG CGAACAAAGT GCGCTTTCAC CTCAGTAAGG ACGAGCTGGA GCGGCAGATG 
0061 AGGAAAATTC ACGACCGTGA GGAATCCGTA GACGAAAAAA TGCGTCCATC AGGAAGCGAA 
0121 AACAGCGGTC ATAATTGCAA TACGAGCTCG TCCTCAAGTC TGCCACGGCA GGAGTCCTGG 
0181 ATCTTCGAGA GCGTTGGGGG TTACTTTCTC GCCAACGAAA ATTTGGCCAA GATGAGTTTG 
0241 CCTTGTCAGG AACAGTTGCT GATGAGGGAT CTAATCTATG CCTTCTCGGG TGTTCCATCT 
0301 GCATATGTAA AGCCTGATAT TCAGATCGAT CAGATTTCCG AAATGTCTGC AGTGGATATA 
0361 GCCAAGATTC GCTTTCGATT GAAAAAGACC TTCACTGGGG CTTTTAGGGC TTTGGCCAAT 
0421 GAAACATTAC CCCTGATAGG TTATTATATT AGTGTACAGA GCTATATAGA GGAGACCAAT 
0481 ATGTCGCCTA ACTGCGGAAG  
 Assemble Data 
<PRE>

7716R-3.IR_full       1   AGTGACTTCGCGAACAAAGTGCGCTTTCACCTCAGTAAGGACGAGCTGGAGCGGCAGATG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7716R-3.in silico     1   AGTGACTTCGCGAACAAAGTGCGCTTTCACCTCAGTAAGGACGAGCTGGAGCGGCAGATG 60


7716R-3.IR_full       61  AGGAAAATTCACGACCGTGAGGAATCCGTAGACGAAAAAATGCGTCCATCAGGAAGCGAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7716R-3.in silico     61  AGGAAAATTCACGACCGTGAGGAATCCGTAGACGAAAAAATGCGTCCATCAGGAAGCGAA 120


7716R-3.IR_full       121 AACAGCGGTCATAATTGCAATACGAGCTCGTCCTCAAGTCTGCCACGGCAGGAGTCCTGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7716R-3.in silico     121 AACAGCGGTCATAATTGCAATACGAGCTCGTCCTCAAGTCTGCCACGGCAGGAGTCCTGG 180


7716R-3.IR_full       181 ATCTTCGAGAGCGTTGGGGGTTACTTTCTCGCCAACGAAAATTTGGCCAAGATGAGTTTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7716R-3.in silico     181 ATCTTCGAGAGCGTTGGGGGTTACTTTCTCGCCAACGAAAATTTGGCCAAGATGAGTTTG 240


7716R-3.IR_full       241 CCTTGTCAGGAACAGTTGCTGATGAGGGATCTAATCTATGCCTTCTCGGGTGTTCCATCT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7716R-3.in silico     241 CCTTGTCAGGAACAGTTGCTGATGAGGGATCTAATCTATGCCTTCTCGGGTGTTCCATCT 300


7716R-3.IR_full       301 GCATATGTAAAGCCTGATATTCAGATCGATCAGATTTCCGAAATGTCTGCAGTGGATATA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7716R-3.in silico     301 GCATATGTAAAGCCTGATATTCAGATCGATCAGATTTCCGAAATGTCTGCAGTGGATATA 360


7716R-3.IR_full       361 GCCAAGATTCGCTTTCGATTGAAAAAGACCTTCACTGGGGCTTTTAGGGCTTTGGCCAAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7716R-3.in silico     361 GCCAAGATTCGCTTTCGATTGAAAAAGACCTTCACTGGGGCTTTTAGGGCTTTGGCCAAT 420


7716R-3.IR_full       421 GAAACATTACCCCTGATAGGTTATTATATTAGTGTACAGAGCTATATAGAGGAGACCAAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7716R-3.in silico     421 GAAACATTACCCCTGATAGGTTATTATATTAGTGTACAGAGCTATATAGAGGAGACCAAT 480


7716R-3.IR_full       481 ATGTCGCCTAACTGCGGAAG 500
                          ||||||||||||||||||||
7716R-3.in silico     481 ATGTCGCCTAACTGCGGAAG 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_139882.1  CG7716-RA (CG7716), mRNA 
0.2   1  2  2  2  NM_141554.2  CG8032-RA (CG8032), mRNA 
0   0  1  3  2  NM_140352.1  CG10943-RA (CG10943), mRNA 
0   0  1  2  2  NM_057659.2  CG9652-RA (DopR), mRNA 
0   0  0  6  2  NM_139526.2  CG14965-RA (CG14965), mRNA 
0   0  0  4  4  NM_141758.3  CG6544-RA, transcript variant A (fau), mRNA 
0   0  0  4  3  NM_057878.3  CG5179-RA (Cdk9), mRNA 
0   0  0  4  3  NM_140803.1  CG14073-RB, transcript variant B (CG14073), mRNA 
0   0  0  4  3  NM_168776.1  CG14073-RA, transcript variant A (CG14073), mRNA 
0   0  0  4  3  NM_001014492.1  CG33511-RA (CG33511), mRNA 
0   0  0  4  2  NM_057768.2  CG7779-RA (Cng), mRNA 
0   0  0  3  8  NM_001043013.1  CG17800-PBD (Dscam), mRNA 
0   0  0  3  7  NM_138079.1  CG13582-RA (CG13582), mRNA 
0   0  0  3  6  NM_169225.1  CG31259-RA (CG31259), mRNA 
0   0  0  3  6  NM_057826.3  CG10043-RA, transcript variant A (rtGEF), mRNA 
0   0  0  3  6  NM_165327.1  CG10043-RB, transcript variant B (rtGEF), mRNA 
0   0  0  3  5  NM_001043024.1  CG17800-PBC (Dscam), mRNA 
0   0  0  3  4  NM_132872.1  CG12698-RA (CG12698), mRNA 
0   0  0  3  4  NM_140267.1  CG14126-RA (CG14126), mRNA 
0   0  0  3  4  NM_206142.1  CG33455-RA (CG33455), mRNA 
0   0  0  3  4  NM_078698.2  CG1676-RA (cactin), mRNA 
0   0  0  3  4  NM_134769.2  CG17642-RA (mRpL48), mRNA 
0   0  0  3  3  NM_165132.1  CG18109-RA (CG18109), mRNA 
0   0  0  3  3  NM_057804.2  CG1451-RA (Apc), mRNA 
0   0  0  3  3  NM_165401.1  CG31600-RA (CG31600), mRNA 
0   0  0  3  3  NM_140170.1  CG14145-RA (CG14145), mRNA 
0   0  0  3  2  NM_001043279.1  CG34131-RA, transcript variant A (CG34131), mRNA 
0   0  0  3  2  NM_142770.2  CG7071-RA, transcript variant A (CG7071), mRNA 
0   0  0  3  2  NM_206543.1  CG7071-RB, transcript variant B (CG7071), mRNA 
0   0  0  3  2  NM_140719.2  CG3885-RA (CG3885), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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