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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7692R-2

Symbol

CG7692

Full Name

CG7692

CG No

CG7692

Old CG No

CG7692

Synonyms

CG7692

Accession No (Link to NCBI)

NM_140715.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCCTGGATTGTGCGGAGAAAC

Primer Seq. 3'

aatctagaggtaccGCCGGCATTAAAGAGCTGTAT

Predicted Fragment Size

IR fragment full Seq

0001 TCCTGGATTG TGCGGAGAAA CTGGAGTACG ATGAGTTCTC GGCGAAAATG TTTGTATTCT 
0061 GCAATGCGCA CAGCGATGTG GATGTGTTTG AGCTAATGGG CAATGACCGG TTGTCGCAGT 
0121 TAATCTACGG ATGGGAGGTG TGCCATCTGC CCACCAGGGA TTCCGAGTCC TTAAAGGACC 
0181 CAGCGCTGCG TCACATGGTG CGGGTTTTAG CGCACCACTC GCTGCTGATT TGGTTTAGCC 
0241 AGGAGTGGAA GACCTGCTCC GAAGGCCTTA AGCAACTATT GCTCTACAGC CTCACCAAAG 
0301 CGGTGCCGTC GTTCTACCAG ATTGGTGAAC TGGCCAATTT CAACTGGTCG TACGTTCTGC 
0361 AGTTCCAGAC AAGGCCCTGG AGCCTGCCCT ACCTGCAGCA GCTATTCCTT AACCTGAAGC 
0421 TGAACAAACA CCACGCCATC GAGCACCCAG TTCCTGCGGC ATCAGGAGAA GAAGTTGCTT 
0481 TCCTGATACA GGAAGGGATA TCTCTGGCCC TGCTGCGGCT AATACAGCTC TTTAATGCCG 
0541 GC

in silico PCR Fragment

0001 TCCTGGATTG TGCGGAGAAA CTGGAGTACG ATGAGTTCTC GGCGAAAATG TTTGTATTCT 
0061 GCAATGCGCA CAGCGATGTG GATGTGTTTG AGCTAATGGG CAATGACCGG TTGTCGCAGT 
0121 TAATCTACGG ATGGGAGGTG TGCCATCTGC CCACCAGGGA TTCCGAGTCC TTAAAGGACC 
0181 CAGCGCTGCG TCACATGGTG CGGGTTTTAG CGCACCACTC GCTGCTGATT TGGTTTAGCC 
0241 AGGAGTGGAA GACCTGCTCC GAAGGCCTTA AGCAACTATT GCTCTACAGC CTCACCAAAG 
0301 CGGTGCCGTC GTTCTACCAG ATTGGTGAAC TGGCCAATTT CAACTGGTCG TACGTTCTGC 
0361 AGTTCCAGAC AAGGCCCTGG AGCCTGCCCT ACCTGCAGCA GCTATTCCTT AACCTGAAGC 
0421 TGAACAAACA CCACGCCATC GAGCACCCAG TTCCTGCGGC ATCAGGAGAA GAAGTTGCTT 
0481 TCCTGATACA GGAAGGGATA TCTCTGGCCC TGCTGCGGCT AATACAGCTC TTTAATGCCG 
0541 GC

Assemble Data

<PRE>

7692R-2.IR_full       1   TCCTGGATTGTGCGGAGAAACTGGAGTACGATGAGTTCTCGGCGAAAATGTTTGTATTCT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7692R-2.in silico     1   TCCTGGATTGTGCGGAGAAACTGGAGTACGATGAGTTCTCGGCGAAAATGTTTGTATTCT 60


7692R-2.IR_full       61  GCAATGCGCACAGCGATGTGGATGTGTTTGAGCTAATGGGCAATGACCGGTTGTCGCAGT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7692R-2.in silico     61  GCAATGCGCACAGCGATGTGGATGTGTTTGAGCTAATGGGCAATGACCGGTTGTCGCAGT 120


7692R-2.IR_full       121 TAATCTACGGATGGGAGGTGTGCCATCTGCCCACCAGGGATTCCGAGTCCTTAAAGGACC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7692R-2.in silico     121 TAATCTACGGATGGGAGGTGTGCCATCTGCCCACCAGGGATTCCGAGTCCTTAAAGGACC 180


7692R-2.IR_full       181 CAGCGCTGCGTCACATGGTGCGGGTTTTAGCGCACCACTCGCTGCTGATTTGGTTTAGCC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7692R-2.in silico     181 CAGCGCTGCGTCACATGGTGCGGGTTTTAGCGCACCACTCGCTGCTGATTTGGTTTAGCC 240


7692R-2.IR_full       241 AGGAGTGGAAGACCTGCTCCGAAGGCCTTAAGCAACTATTGCTCTACAGCCTCACCAAAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7692R-2.in silico     241 AGGAGTGGAAGACCTGCTCCGAAGGCCTTAAGCAACTATTGCTCTACAGCCTCACCAAAG 300


7692R-2.IR_full       301 CGGTGCCGTCGTTCTACCAGATTGGTGAACTGGCCAATTTCAACTGGTCGTACGTTCTGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7692R-2.in silico     301 CGGTGCCGTCGTTCTACCAGATTGGTGAACTGGCCAATTTCAACTGGTCGTACGTTCTGC 360


7692R-2.IR_full       361 AGTTCCAGACAAGGCCCTGGAGCCTGCCCTACCTGCAGCAGCTATTCCTTAACCTGAAGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7692R-2.in silico     361 AGTTCCAGACAAGGCCCTGGAGCCTGCCCTACCTGCAGCAGCTATTCCTTAACCTGAAGC 420


7692R-2.IR_full       421 TGAACAAACACCACGCCATCGAGCACCCAGTTCCTGCGGCATCAGGAGAAGAAGTTGCTT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7692R-2.in silico     421 TGAACAAACACCACGCCATCGAGCACCCAGTTCCTGCGGCATCAGGAGAAGAAGTTGCTT 480


7692R-2.IR_full       481 TCCTGATACAGGAAGGGATATCTCTGGCCCTGCTGCGGCTAATACAGCTCTTTAATGCCG 540
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7692R-2.in silico     481 TCCTGATACAGGAAGGGATATCTCTGGCCCTGCTGCGGCTAATACAGCTCTTTAATGCCG 540


7692R-2.IR_full       541 GC 542
                          ||
7692R-2.in silico     541 GC 542

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	524	0	0	3	NM_140715.2	CG7692-RA (CG7692), mRNA
11.64	61	154	170	76	NM_132905.2	CG9902-RA (CG9902), mRNA
0	0	2	11	2	NM_132986.4	CG8611-RA, transcript variant A (CG8611), mRNA
0	0	2	11	2	NM_176749.1	CG8611-RB, transcript variant B (CG8611), mRNA
0	0	2	6	4	NM_057595.3	CG5848-RC, transcript variant C (cact), mRNA
0	0	2	6	4	NM_057594.3	CG5848-RB, transcript variant B (cact), mRNA
0	0	2	3	7	NM_001014683.1	CG2005-RD, transcript variant D (Ptp99A), mRNA
0	0	2	3	7	NM_170409.1	CG2005-RA, transcript variant A (Ptp99A), mRNA
0	0	2	3	7	NM_001043332.1	CG2005-RE, transcript variant E (Ptp99A), mRNA
0	0	2	3	5	NM_057468.2	CG2005-RB, transcript variant B (Ptp99A), mRNA
0	0	2	2	3	NM_142931.3	CG10192-RA (CG10192), mRNA
0	0	2	2	3	NM_132302.3	CG7055-RA (dalao), mRNA
0	0	2	2	2	NM_141207.3	CG9853-RA (CG9853), mRNA
0	0	1	5	4	NM_132009.2	CG11473-RA (CG11473), mRNA
0	0	1	3	6	NM_078604.3	CG32592-RA (hiw), mRNA
0	0	1	3	4	NM_057294.4	CG11680-RB, transcript variant B (mle), mRNA
0	0	1	3	4	NM_057293.3	CG11680-RA, transcript variant A (mle), mRNA
0	0	1	3	4	NM_165451.2	CG11680-RC, transcript variant C (mle), mRNA
0	0	1	3	3	NM_078727.2	CG4426-RA (ast), mRNA
0	0	1	2	6	NM_139493.2	CG2083-RA (CG2083), mRNA
0	0	1	2	4	NM_164933.1	CG7438-RB, transcript variant B (Myo31DF), mRNA
0	0	1	2	3	NM_078814.2	CG7438-RA, transcript variant A (Myo31DF), mRNA
0	0	1	2	3	NM_079066.2	CG15110-RA (botv), mRNA
0	0	1	2	2	NM_206544.1	CG33336-RB, transcript variant B (p53), mRNA
0	0	1	2	2	NM_206545.1	CG33336-RA, transcript variant A (p53), mRNA
0	0	0	6	5	NM_176218.1	CG33130-RA, transcript variant A (l(2)k07433), mRNA
0	0	0	6	5	NM_206154.1	CG33130-RB, transcript variant B (l(2)k07433), mRNA
0	0	0	6	5	NM_206153.1	CG33130-RC, transcript variant C (l(2)k07433), mRNA
0	0	0	5	9	NM_169259.1	CG9755-RD, transcript variant D (pum), mRNA
0	0	0	5	9	NM_079561.2	CG9755-RC, transcript variant C (pum), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

Pathways (updated: 2024/04/26)

KEGG Pathway

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