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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7656R-2

Symbol

CG7656

Full Name

CG7656

CG No

CG7656

Old CG No

CG7656

Synonyms

CG7656

Accession No (Link to NCBI)

NM_168628.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Nakaoka T, Iga M, Yamada T, Koujima I, Takeshima M, Zhou X, Suzuki Y, Ogihara MH, Kataoka H.<br> Deep sequencing of the prothoracic gland transcriptome reveals new players in insect ecdysteroidogenesis.<br> PLoS One (2017) 12(3) e0172951 [ PubMed ID = 28257485 ] [ RRC reference ]

Strutt H, Searle E, Thomas-Macarthur V, Brookfield R, Strutt D.<br> A Cul-3-BTB ubiquitylation pathway regulates junctional levels and asymmetry of core planar polarity proteins.<br> Development (2013) 140(8) 1693-702 [ PubMed ID = 23487316 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCATCGCAGCATGCGACATCGT

Primer Seq. 3'

aatctagaggtaccGTTGGCCGGCGAGAATGTGTT

Predicted Fragment Size

IR fragment full Seq

0001 CATcGCAGCA TGCGACATCG TCCTCCACAA TGATGGCGGG CTCTGGATCC TTGGCGACCT 
0061 CGTCGTCCGC CGCTGCCCCC ACAACCACGC CCAGCAGCTC GGCCGTTCGC GCCCTGGCCA 
0121 TGGAGTACAA GTCGCTGCAG GAAGAGCCGG TGGAGGGATT CCGCGTCAAG CTGATCAACG 
0181 ACGACAATCT GTTCGAATGG GAGGTGGCAA TCTTCGGGCC ACCGGACACC CTCTACCAGG 
0241 GCGGCTACTT CAAGGCACAC ATGAAGTTCC CGCACGACTA TCCATACTCA CCGCCATCCA 
0301 TACGCTTCCT GACGAAGGTC TGGCATCCGA ATGTTTACGA GAACGGTGAT CTGTGCATAT 
0361 CCATACTACA TCCGCCGGTG GATGATCCGC AGAGTGGCGA GCTGCCCTGC GAACGCTGGN 
0421 AATCCCACGC AGAATGTGCG CACCATCCTG CTGTCGGTCA TATCGCTGCT CAATGAGCCG 
0481 AACACATTCT CGCCGGCCAA C

in silico PCR Fragment

0001 CATCGCAGCA TGCGACATCG TCCTCCACAA TGATGGCGGG CTCTGGATCC TTGGCGACCT 
0061 CGTCGTCCGC CGCTGCCCCC ACAACCACGC CCAGCAGCTC GGCCGTTCGC GCCCTGGCCA 
0121 TGGAGTACAA GTCGCTGCAG GAAGAGCCGG TGGAGGGATT CCGCGTCAAG CTGATCAACG 
0181 ACGACAATCT GTTCGAATGG GAGGTGGCAA TCTTCGGGCC ACCGGACACC CTCTACCAGG 
0241 GCGGCTACTT CAAGGCGCAC ATGAAGTTCC CGCACGACTA TCCATACTCA CCGCCATCCA 
0301 TACGCTTCCT GACGAAGGTC TGGCATCCGA ATGTTTACGA GAACGGTGAT CTGTGCATAT 
0361 CCATACTACA TCCGCCGGTG GATGATCCGC AGAGTGGCGA GCTGCCCTGC GAACGCTGG- 
0421 AATCCCACGC AGAATGTGCG CACCATCCTG CTGTCGGTCA TATCGCTGCT CAATGAGCCG 
0481 AACACATTCT CGCCGGCCAA C

Assemble Data

<PRE>

7656R-2.IR_full       1   CATCGCAGCATGCGACATCGTCCTCCACAATGATGGCGGGCTCTGGATCCTTGGCGACCT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7656R-2.in silico     1   CATCGCAGCATGCGACATCGTCCTCCACAATGATGGCGGGCTCTGGATCCTTGGCGACCT 60


7656R-2.IR_full       61  CGTCGTCCGCCGCTGCCCCCACAACCACGCCCAGCAGCTCGGCCGTTCGCGCCCTGGCCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7656R-2.in silico     61  CGTCGTCCGCCGCTGCCCCCACAACCACGCCCAGCAGCTCGGCCGTTCGCGCCCTGGCCA 120


7656R-2.IR_full       121 TGGAGTACAAGTCGCTGCAGGAAGAGCCGGTGGAGGGATTCCGCGTCAAGCTGATCAACG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7656R-2.in silico     121 TGGAGTACAAGTCGCTGCAGGAAGAGCCGGTGGAGGGATTCCGCGTCAAGCTGATCAACG 180


7656R-2.IR_full       181 ACGACAATCTGTTCGAATGGGAGGTGGCAATCTTCGGGCCACCGGACACCCTCTACCAGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7656R-2.in silico     181 ACGACAATCTGTTCGAATGGGAGGTGGCAATCTTCGGGCCACCGGACACCCTCTACCAGG 240


7656R-2.IR_full       241 GCGGCTACTTCAAGGC<span class="snp"><tt>A</tt></span>CACATGAAGTTCCCGCACGACTATCCATACTCACCGCCATCCA 300
                          ||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||
7656R-2.in silico     241 GCGGCTACTTCAAGGC<span class="snp"><tt>G</tt></span>CACATGAAGTTCCCGCACGACTATCCATACTCACCGCCATCCA 300


7656R-2.IR_full       301 TACGCTTCCTGACGAAGGTCTGGCATCCGAATGTTTACGAGAACGGTGATCTGTGCATAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7656R-2.in silico     301 TACGCTTCCTGACGAAGGTCTGGCATCCGAATGTTTACGAGAACGGTGATCTGTGCATAT 360


7656R-2.IR_full       361 CCATACTACATCCGCCGGTGGATGATCCGCAGAGTGGCGAGCTGCCCTGCGAACGCTGG<span class="snp"><tt>N</tt></span> 420
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
7656R-2.in silico     361 CCATACTACATCCGCCGGTGGATGATCCGCAGAGTGGCGAGCTGCCCTGCGAACGCTGG<span class="snp"><tt>-</tt></span> 420


7656R-2.IR_full       421 AATCCCACGCAGAATGTGCGCACCATCCTGCTGTCGGTCATATCGCTGCTCAATGAGCCG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7656R-2.in silico     421 AATCCCACGCAGAATGTGCGCACCATCCTGCTGTCGGTCATATCGCTGCTCAATGAGCCG 480


7656R-2.IR_full       481 AACACATTCTCGCCGGCCAAC 501
                          |||||||||||||||||||||
7656R-2.in silico     481 AACACATTCTCGCCGGCCAAC 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_168628.1	CG7656-RA, transcript variant A (CG7656), mRNA
100	482	0	0	0	NM_168627.1	CG7656-RC, transcript variant C (CG7656), mRNA
66.59	321	3	2	2	NM_140526.3	CG7656-RB, transcript variant B (CG7656), mRNA
0	0	5	3	2	NM_001014577.1	CG6494-RB, transcript variant B (h), mRNA
0	0	5	3	2	NM_079253.3	CG6494-RA, transcript variant A (h), mRNA
0	0	4	2	4	NM_140325.1	CG10682-RA (vih), mRNA
0	0	3	4	4	NM_141335.1	CG11000-RA (CG11000), mRNA
0	0	3	2	3	NM_168802.1	CG32210-RA (CG32210), mRNA
0	0	3	2	2	NM_170333.1	CG5938-RD, transcript variant D (CG5938), mRNA
0	0	3	2	2	NM_170332.1	CG5938-RB, transcript variant B (CG5938), mRNA
0	0	3	2	2	NM_143296.2	CG5938-RC, transcript variant C (CG5938), mRNA
0	0	3	2	2	NM_170331.1	CG5938-RA, transcript variant A (CG5938), mRNA
0	0	2	4	3	NM_140438.2	CG32139-RA (Sox21b), mRNA
0	0	2	3	3	NM_168874.1	CG4365-RB, transcript variant B (CG4365), mRNA
0	0	2	3	3	NM_168873.1	CG4365-RC, transcript variant C (CG4365), mRNA
0	0	2	3	3	NM_141001.2	CG4365-RA, transcript variant A (CG4365), mRNA
0	0	2	2	2	NM_135127.1	CG9044-RA (CG9044), mRNA
0	0	1	8	19	NM_142052.1	CG9602-RA (CG9602), mRNA
0	0	1	5	9	NM_057643.2	CG10844-RA, transcript variant A (Rya-r44F), mRNA
0	0	1	5	9	NM_057644.2	CG10844-RB, transcript variant B (Rya-r44F), mRNA
0	0	1	5	9	NM_057645.2	CG10844-RC, transcript variant C (Rya-r44F), mRNA
0	0	1	5	9	NM_057646.2	CG10844-RD, transcript variant D (Rya-r44F), mRNA
0	0	1	5	3	NM_164543.1	CG31954-RA (CG31954), mRNA
0	0	1	4	13	NM_206278.1	CG5406-RC, transcript variant C (sif), mRNA
0	0	1	4	13	NM_079908.2	CG5406-RA, transcript variant A (sif), mRNA
0	0	1	4	8	NM_168126.1	CG5406-RB, transcript variant B (sif), mRNA
0	0	1	4	3	NM_079770.2	CG7748-RA (OstStt3), mRNA
0	0	1	4	3	NM_169272.1	CG18473-RA (CG18473), mRNA
0	0	1	3	3	NM_080245.3	CG17437-RA (wds), mRNA
0	0	1	3	2	NM_131951.1	CG15473-RA (CG15473), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme04120 : Ubiquitin mediated proteolysis

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