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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7574R-1

Symbol bip1 Full Name bip1

CG No CG7574 Old CG No CG7574

Synonyms bip-I, CG7574, anon-WO0118547.246, bip1

Accession No (Link to NCBI) NM_139912.3

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACCACTCTTCGGATTTGCCAG

Primer Seq. 3'

aatctagaggtaccATGGGCATAATGCCGGTGTTG

Predicted Fragment Size

IR fragment full Seq

0001 ACCACTCTTC GGATTTGCCA GGCTGTGTCA GCTAAATCCT AGCGCGAATG GGCCGGAACC 
0061 AGTATTGCCA CCTTCCTCCA GGATTTGTCT GATGGGTTGC CAGGATGTAA AGCCCAATGA 
0121 TCTGCGTCGT TTTCCAGTCC ACGATACCGA TCGCTGCAAT CATTGGCTGC GTCACTTTGG 
0181 CGTCAGCTAT ANACTGGTGG ATCAACTCGG CGGCCTCCAA AAACTGGCGC ATCTGCTCCA 
0241 GACACTTTAG CCAACGCCAG GAGATGGCCA AAACCCGCAT CAAGATGACG CcgCTGCGCA 
0301 AGTCCTCGTC CTCCAAGGGC ATTGTGCTAC CCATTAATGN CGCTGGAGGG TCGGTCATTG 
0361 CAGGCGCCTT AGCAGGAGGA GGAGGTGCAG GAACTGGGAG CGGTGCTGTC AAGTCGGCGG 
0421 TGGCCAGCAG CACCACCATT GAACTTGTTG AGAGCCCCAA TAGACATCAG GCCAAGAAGC 
0481 CCAACACCGG CATTATGCCC AT

in silico PCR Fragment

0001 ACCACTCTTC GGATTTGCCA GGCTGTGTC- GCTAAATCCT AGCGCGAATG GGCCGGAACC 
0061 AGTATTGCCA CCTTCCTCCA GGATTTGTCT GATGGGTTGC CAGGATGTAA AGCCCAATGA 
0121 TCTGCGTCGT TTTCCAGTCC ACGATACCGA TCGCTGCAAT CATTGGCTGC GTCACTTTGG 
0181 CGTCAGCTAT AAACTGGTGG ATCAACTCGG CGGCCTCCAA AAGCT-GCGC ATCTGCTCCA 
0241 GACACTTTAG CCAACGCCAG GAGATGGCCA AAACCCGCAT CAAGATGACG CCGCTGCGCA 
0301 AGTCCTCGTC CTCCAAGGGC ATTGTGCTAC CCATTAATGC CGCTGGAGGG TCGGTCATTG 
0361 CAGGCGCCTT AGCAGGAGGA GGAGGTGCAG GAACTGGGAG CGGTGCTGTC AAGTCGGCGG 
0421 TGGCCAGCAG CACCACCATT GAACTTGTTG AGAGCCCCAA TAGACATCAG GCCAAGAAGC 
0481 CCAACACCGG CATTATGCCC AT

Assemble Data


7574R-1.IR_full       1   ACCACTCTTCGGATTTGCCAGGCTGTGTCAGCTAAATCCTAGCGCGAATGGGCCGGAACC 60
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
7574R-1.in silico     1   ACCACTCTTCGGATTTGCCAGGCTGTGTC-GCTAAATCCTAGCGCGAATGGGCCGGAACC 60


7574R-1.IR_full       61  AGTATTGCCACCTTCCTCCAGGATTTGTCTGATGGGTTGCCAGGATGTAAAGCCCAATGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7574R-1.in silico     61  AGTATTGCCACCTTCCTCCAGGATTTGTCTGATGGGTTGCCAGGATGTAAAGCCCAATGA 120


7574R-1.IR_full       121 TCTGCGTCGTTTTCCAGTCCACGATACCGATCGCTGCAATCATTGGCTGCGTCACTTTGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7574R-1.in silico     121 TCTGCGTCGTTTTCCAGTCCACGATACCGATCGCTGCAATCATTGGCTGCGTCACTTTGG 180


7574R-1.IR_full       181 CGTCAGCTATANACTGGTGGATCAACTCGGCGGCCTCCAAAAACTGGCGCATCTGCTCCA 240
                          ||||||||||| |||||||||||||||||||||||||||||| || ||||||||||||||
7574R-1.in silico     181 CGTCAGCTATAAACTGGTGGATCAACTCGGCGGCCTCCAAAAGCT-GCGCATCTGCTCCA 240


7574R-1.IR_full       241 GACACTTTAGCCAACGCCAGGAGATGGCCAAAACCCGCATCAAGATGACGCCGCTGCGCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7574R-1.in silico     241 GACACTTTAGCCAACGCCAGGAGATGGCCAAAACCCGCATCAAGATGACGCCGCTGCGCA 300


7574R-1.IR_full       301 AGTCCTCGTCCTCCAAGGGCATTGTGCTACCCATTAATGNCGCTGGAGGGTCGGTCATTG 360
                          ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
7574R-1.in silico     301 AGTCCTCGTCCTCCAAGGGCATTGTGCTACCCATTAATGCCGCTGGAGGGTCGGTCATTG 360


7574R-1.IR_full       361 CAGGCGCCTTAGCAGGAGGAGGAGGTGCAGGAACTGGGAGCGGTGCTGTCAAGTCGGCGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7574R-1.in silico     361 CAGGCGCCTTAGCAGGAGGAGGAGGTGCAGGAACTGGGAGCGGTGCTGTCAAGTCGGCGG 420


7574R-1.IR_full       421 TGGCCAGCAGCACCACCATTGAACTTGTTGAGAGCCCCAATAGACATCAGGCCAAGAAGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7574R-1.in silico     421 TGGCCAGCAGCACCACCATTGAACTTGTTGAGAGCCCCAATAGACATCAGGCCAAGAAGC 480


7574R-1.IR_full       481 CCAACACCGGCATTATGCCCAT 502
                          ||||||||||||||||||||||
7574R-1.in silico     481 CCAACACCGGCATTATGCCCAT 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139912.3	CG7574-RA (bip1), mRNA
0	0	7	3	2	NM_142620.1	CG15684-RA (CG15684), mRNA
0	0	4	6	4	NM_137123.2	CG17390-RA (CG17390), mRNA
0	0	4	2	4	NM_134983.2	CG4104-RA (Tps1), mRNA
0	0	3	4	7	NM_145192.2	CG15107-RA (CG15107), mRNA
0	0	3	4	5	NM_138073.1	CG13579-RA (CG13579), mRNA
0	0	3	3	3	NM_136700.2	CG1407-RB, transcript variant B (CG1407), mRNA
0	0	3	3	3	NM_165728.2	CG1407-RA, transcript variant A (CG1407), mRNA
0	0	3	3	3	NM_205956.1	CG33302-RA (CG33302), mRNA
0	0	3	3	2	NM_080707.2	CG3430-RA (CG3430), mRNA
0	0	3	2	6	NM_079711.2	CG6545-RA (lbe), mRNA
0	0	3	2	4	NM_139676.1	CG13708-RA (CG13708), mRNA
0	0	2	5	5	NM_078717.2	CG3696-RA, transcript variant A (kis), mRNA
0	0	2	5	5	NM_164376.1	CG3696-RB, transcript variant B (kis), mRNA
0	0	2	3	4	NM_169351.1	CG4067-RC, transcript variant C (pug), mRNA
0	0	2	3	4	NM_057906.3	CG4067-RA, transcript variant A (pug), mRNA
0	0	2	3	4	NM_001014614.1	CG4067-RD, transcript variant D (pug), mRNA
0	0	2	3	4	NM_169350.1	CG4067-RB, transcript variant B (pug), mRNA
0	0	2	3	3	NM_080359.3	CG11822-RA, transcript variant A (nAcRbeta-21C), mRNA
0	0	2	3	3	NM_164386.2	CG11822-RB, transcript variant B (nAcRbeta-21C), mRNA
0	0	2	3	2	NM_132735.2	CG12540-RA (CG12540), mRNA
0	0	2	3	2	NM_165628.1	CG30352-RA (CG30352), mRNA
0	0	2	2	4	NM_136443.2	CG1620-RA (CG1620), mRNA
0	0	2	2	3	NM_137871.2	CG3536-RA (CG3536), mRNA
0	0	2	2	2	NM_166551.2	CG30267-RA (CG30267), mRNA
0	0	1	9	26	NM_167685.1	CG12701-RB, transcript variant B (CG12701), mRNA
0	0	1	9	26	NM_134512.4	CG12701-RA, transcript variant A (CG12701), mRNA
0	0	1	7	22	NM_132012.1	CG15776-RA (CG15776), mRNA
0	0	1	7	16	NM_167198.1	CG32697-RB, transcript variant B (l(1)G0232), mRNA
0	0	1	7	16	NM_132348.2	CG32697-RA, transcript variant A (l(1)G0232), mRNA

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