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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7565R-2

Symbol CG7565 Full Name CG7565

CG No CG7565 Old CG No CG7565

Synonyms CT23145, CG7565

Accession No (Link to NCBI) NM_139914.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees male lethal

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAAACACAAGGAGACCAGCCC

Primer Seq. 3'

aatctagaggtaccCCTGATTGCGAGCCAGGTAGC

Predicted Fragment Size

IR fragment full Seq

0001 NAAACACAAG GAGACCAGCC CCGATAATTC AGTTGGCGGT TCTATATCGC CCAATTTGGT 
0061 GTGCCACAAN GATGCTGAGG CATGTGTTCG AAAACGCCAC TCCGCGGGAT GAGCAGCAGG 
0121 CGGGTGTGTT TGAGGAGTAC AAGCCACCGC CGGATGCAGT GGAGCCACTG GAGGAGGAGG 
0181 CCTATCTTTG GAACTGCCTA CAGGCTTGCT GCGAAAAGCC CCGGAATGGC AGCAGTGCCT 
0241 GCAATGTGGT CCTCGTTTTT AAGGCCAAAT GCTACCATAT CCGATGCCAG AGCAACGAGG 
0301 CCTGCTTGCC CAAGCTCAGA GTTCGGATGC CCAACGAAAA GGTGCAAATG GTACTGGTCA 
0361 ATCCACTGGG CGATGcCACA TGGCCACAGC TCCTCAAGGC GGAGGCAGCC AAACAGAATG 
0421 CTGAGATTCT GCCATACGAT GAAGCGGCAC TTAATTTCTG GAAGCAGCCC AGAAGGTTGA 
0481 GCTACCTGGC TCGCAATCAG G

in silico PCR Fragment

0001 GAAACACAAG GAGACCAGCC CCGATAATTC AGTTGGCGGT TCTATATCGC CCAATTTGGT 
0061 GTGCCACAA- GATGCTGAGG CATGTGTTCG AAAACGCCAC TCCGCGGGAT GAGCAGCAGG 
0121 CGGGTGTGTT TGAGGAGTAC AAGCCACCGC CGGATGCAGT GGAGCCACTG GAGGAGGAGG 
0181 CCTATCTTTG GAACTGCCTA CAGGCTTGCT GCGAAAAGCC CCGGAATGGC AGCAGTGCCT 
0241 GCAATGTGGT CCTCGTTTTT AAGGCCAAAT GCTACCATAT CCGATGCCAG AGCAACGAGG 
0301 CCTGCTTGCC CAAGCTCAGA GTTCGGATGC CCAACGAAAA GGTTCAGATG GTACTGGTCA 
0361 ATCCACTGGG CGATGCCACA TGGCCACAGC TCCTCAAGGC GGAGGCAGCC AAACAGAATG 
0421 CTGAGATTCT GCCATACGAT GAAGCGGCAC TTAATTTCTG GAAGCAGCCC AGAAGGTTGA 
0481 GCTACCTGGC TCGCAATCAG G

Assemble Data


7565R-2.IR_full        1   AAACACAAGGAGACCAGCCCCGATAATTCAGTTGGCGGTTCTATATCGCCCAATTTGGT 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7565R-2.in silico      1   AAACACAAGGAGACCAGCCCCGATAATTCAGTTGGCGGTTCTATATCGCCCAATTTGGT 59


7565R-2.IR_full       61  GTGCCACAANGATGCTGAGGCATGTGTTCGAAAACGCCACTCCGCGGGATGAGCAGCAGG 119
                          ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
7565R-2.in silico     61  GTGCCACAA-GATGCTGAGGCATGTGTTCGAAAACGCCACTCCGCGGGATGAGCAGCAGG 119


7565R-2.IR_full       121 CGGGTGTGTTTGAGGAGTACAAGCCACCGCCGGATGCAGTGGAGCCACTGGAGGAGGAGG 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7565R-2.in silico     121 CGGGTGTGTTTGAGGAGTACAAGCCACCGCCGGATGCAGTGGAGCCACTGGAGGAGGAGG 179


7565R-2.IR_full       181 CCTATCTTTGGAACTGCCTACAGGCTTGCTGCGAAAAGCCCCGGAATGGCAGCAGTGCCT 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7565R-2.in silico     181 CCTATCTTTGGAACTGCCTACAGGCTTGCTGCGAAAAGCCCCGGAATGGCAGCAGTGCCT 239


7565R-2.IR_full       241 GCAATGTGGTCCTCGTTTTTAAGGCCAAATGCTACCATATCCGATGCCAGAGCAACGAGG 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7565R-2.in silico     241 GCAATGTGGTCCTCGTTTTTAAGGCCAAATGCTACCATATCCGATGCCAGAGCAACGAGG 299


7565R-2.IR_full       301 CCTGCTTGCCCAAGCTCAGAGTTCGGATGCCCAACGAAAAGGTGCAAATGGTACTGGTCA 359
                          ||||||||||||||||||||||||||||||||||||||||||| || |||||||||||||
7565R-2.in silico     301 CCTGCTTGCCCAAGCTCAGAGTTCGGATGCCCAACGAAAAGGTTCAGATGGTACTGGTCA 359


7565R-2.IR_full       361 ATCCACTGGGCGATGCCACATGGCCACAGCTCCTCAAGGCGGAGGCAGCCAAACAGAATG 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7565R-2.in silico     361 ATCCACTGGGCGATGCCACATGGCCACAGCTCCTCAAGGCGGAGGCAGCCAAACAGAATG 419


7565R-2.IR_full       421 CTGAGATTCTGCCATACGATGAAGCGGCACTTAATTTCTGGAAGCAGCCCAGAAGGTTGA 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7565R-2.in silico     421 CTGAGATTCTGCCATACGATGAAGCGGCACTTAATTTCTGGAAGCAGCCCAGAAGGTTGA 479


7565R-2.IR_full       481 GCTACCTGGCTCGCAATCAGG 500
                          |||||||||||||||||||||
7565R-2.in silico     481 GCTACCTGGCTCGCAATCAGG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_139914.2	CG7565-RA (CG7565), mRNA
0	0	2	4	2	NM_206238.1	CG13923-RA (CG13923), mRNA
0	0	2	3	3	NM_143496.3	CG15523-RA (CG15523), mRNA
0	0	2	2	5	NM_001031882.1	CG4523-RD, transcript variant D (Pink1), mRNA
0	0	2	2	5	NM_167083.2	CG4523-RB, transcript variant B (Pink1), mRNA
0	0	2	2	5	NM_132112.3	CG4523-RA, transcript variant A (Pink1), mRNA
0	0	2	2	5	NM_001031883.1	CG4523-RC, transcript variant C (Pink1), mRNA
0	0	2	2	5	NM_001031881.1	CG4523-RE, transcript variant E (Pink1), mRNA
0	0	2	2	5	NM_001031880.1	CG4523-RF, transcript variant F (Pink1), mRNA
0	0	2	2	5	NM_001031879.1	CG4523-RG, transcript variant G (Pink1), mRNA
0	0	2	2	5	NM_001031878.1	CG4523-RH, transcript variant H (Pink1), mRNA
0	0	2	2	4	NM_138025.2	CG3121-RA (CG3121), mRNA
0	0	2	2	3	NM_132423.1	CG11556-RA (Rph), mRNA
0	0	1	3	6	NM_136608.2	CG8777-RA (CG8777), mRNA
0	0	1	3	3	NM_078610.2	CG11678-RA (Actr13E), mRNA
0	0	1	3	2	NM_167487.1	CG32577-RA (disco-r), mRNA
0	0	1	2	3	NM_142121.1	CG7886-RA (CG7886), mRNA
0	0	1	2	3	NM_170430.2	CG31037-RA (ca), mRNA
0	0	1	2	2	NM_079898.2	CG11081-RA, transcript variant A (plexA), mRNA
0	0	1	2	2	NM_166804.2	CG11081-RB, transcript variant B (plexA), mRNA
0	0	1	2	2	NM_166805.2	CG11081-RC, transcript variant C (plexA), mRNA
0	0	1	2	2	NM_166806.2	CG11081-RD, transcript variant D (plexA), mRNA
0	0	0	11	13	NM_170158.1	CG5794-RB, transcript variant B (CG5794), mRNA
0	0	0	11	13	NM_143018.2	CG5794-RD, transcript variant D (CG5794), mRNA
0	0	0	7	16	NM_078529.3	CG18009-RD, transcript variant D (Trf2), mRNA
0	0	0	6	11	NM_176223.2	CG30115-RD, transcript variant D (CG30115), mRNA
0	0	0	6	8	NM_078604.3	CG32592-RA (hiw), mRNA
0	0	0	6	2	NM_136574.2	CG8258-RA (CG8258), mRNA
0	0	0	5	7	NM_176144.1	CG33144-RA (CG33144), mRNA
0	0	0	5	6	NM_136155.2	CG10628-RA (CG10628), mRNA

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