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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7564R-3 |
| Symbol |
CG7564 |
Full Name |
CG7564 |
| CG No |
CG7564 |
Old CG No |
CG7564 |
| Synonyms |
CG7564 |
| Accession No (Link to NCBI) |
NM_140734.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACCACACGGAATGGCGACGAG |
| Primer Seq. 3' |
aatctagaggtaccTGCTGCTGGTAGGTGGATGCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACCACACGGA NATGGCGACG AGCGCCAGCT GAAATTCTCG GACCCCAGGG TCTGCAAGTC
0061 CTTTCTGCTC GACTGCTGTC CGCACGACAT CCTGGCGTCC ACGCGCATGG ATCTCGGTGA
0121 GTGTCCCAAA GTGCATGACC TGGCTTTCCG CGCAGATTAC GAAAGTGCCG CCAAGACGCG
0181 CGACTACTAC TACGACATTG AGGCCATGGA ACACCTGCAG GCCTTTATTG CCGACTGCGA
0241 TCGACGCACC GACTCCGCCA AGCAAAGGTT GAAAGAGACC CAGGAAGAGC TGACCGCCGA
0301 GGTGGCNTGA GAAGGCCAAT GCTGTCCATG GCCTCGCCGA GGNAGATTGG CAAGAAGTTG
0361 GCCAAgGCCG AGGCCCTGGG CGAGGCCGGC GNAAGTGGAG GACAGCATGG AGCTGATGAA
0421 GGAGATCGAG GAGCTGCGTG CTAAGAAGAT CAAGGCCGAG CACGAGTACC GCACATCTAT
0481 GCCCGCATCC AcCTNACCAG CAGCA |
| in silico PCR Fragment |
0001 ACCACACGGA -ATGGCGACG AGCGCCAACT GAAATTCTCG GACCCCAGGG TCTGCAAGTC
0061 CTTTCTGCTC GACTGCTGTC CGCACGACAT CCTGGCGTCC ACGCGCATGG ATCTCGGTGA
0121 GTGTCCCAAA GTGCATGACC TGGCTTTCCG CGCAGATTAC GAAAGTGCCG CCAAGACGCG
0181 CGACTACTAC TACGACATTG AGGCCATGGA ACACCTGCAG GCCTTTATTG CCGACTGCGA
0241 TCGACGCACC GACTCCGCCA AGCAAAGGTT GAAAGAGACC CAGGAAGAGC TGACCGCCGA
0301 GGTGGC-TGA GAAGGCCAAT GCTGTCCATG GCCTCGCCGA GG-AGATTGG CAAGAAGTTG
0361 GCCAAGGCCG AGGCCCTGGG CGAGGCCGGC G-AAGTGGAG GACAGCATGG AGCTGATGAA
0421 GGAGATCGAG GAGCTGCGTG CTAAGAAGAT CAAGGCCGAG CACGAGTACC GCACATCTAT
0481 GCCCGCATCC ACCT-ACCAG CAGCA |
| Assemble Data |
<PRE>
7564R-3.IR_full 1 ACCACACGGA<span class="snp"><tt>N</tt></span>ATGGCGACGAGCGCCA<span class="snp"><tt>G</tt></span>CTGAAATTCTCGGACCCCAGGGTCTGCAAGTC 60
||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||
7564R-3.in silico 1 ACCACACGGA<span class="snp"><tt>-</tt></span>ATGGCGACGAGCGCCA<span class="snp"><tt>A</tt></span>CTGAAATTCTCGGACCCCAGGGTCTGCAAGTC 60
7564R-3.IR_full 61 CTTTCTGCTCGACTGCTGTCCGCACGACATCCTGGCGTCCACGCGCATGGATCTCGGTGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7564R-3.in silico 61 CTTTCTGCTCGACTGCTGTCCGCACGACATCCTGGCGTCCACGCGCATGGATCTCGGTGA 120
7564R-3.IR_full 121 GTGTCCCAAAGTGCATGACCTGGCTTTCCGCGCAGATTACGAAAGTGCCGCCAAGACGCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7564R-3.in silico 121 GTGTCCCAAAGTGCATGACCTGGCTTTCCGCGCAGATTACGAAAGTGCCGCCAAGACGCG 180
7564R-3.IR_full 181 CGACTACTACTACGACATTGAGGCCATGGAACACCTGCAGGCCTTTATTGCCGACTGCGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7564R-3.in silico 181 CGACTACTACTACGACATTGAGGCCATGGAACACCTGCAGGCCTTTATTGCCGACTGCGA 240
7564R-3.IR_full 241 TCGACGCACCGACTCCGCCAAGCAAAGGTTGAAAGAGACCCAGGAAGAGCTGACCGCCGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7564R-3.in silico 241 TCGACGCACCGACTCCGCCAAGCAAAGGTTGAAAGAGACCCAGGAAGAGCTGACCGCCGA 300
7564R-3.IR_full 301 GGTGGC<span class="snp"><tt>N</tt></span>TGAGAAGGCCAATGCTGTCCATGGCCTCGCCGAGG<span class="snp"><tt>N</tt></span>AGATTGGCAAGAAGTTG 360
||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||
7564R-3.in silico 301 GGTGGC<span class="snp"><tt>-</tt></span>TGAGAAGGCCAATGCTGTCCATGGCCTCGCCGAGG<span class="snp"><tt>-</tt></span>AGATTGGCAAGAAGTTG 360
7564R-3.IR_full 361 GCCAAGGCCGAGGCCCTGGGCGAGGCCGGCG<span class="snp"><tt>N</tt></span>AAGTGGAGGACAGCATGGAGCTGATGAA 420
|||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||
7564R-3.in silico 361 GCCAAGGCCGAGGCCCTGGGCGAGGCCGGCG<span class="snp"><tt>-</tt></span>AAGTGGAGGACAGCATGGAGCTGATGAA 420
7564R-3.IR_full 421 GGAGATCGAGGAGCTGCGTGCTAAGAAGATCAAGGCCGAGCACGAGTACCGCACATCTAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7564R-3.in silico 421 GGAGATCGAGGAGCTGCGTGCTAAGAAGATCAAGGCCGAGCACGAGTACCGCACATCTAT 480
7564R-3.IR_full 481 GCCCGCATCCACCT<span class="snp"><tt>N</tt></span>ACCAGCAGCA 505
||||||||||||||<span class="snp"><tt> </tt></span>||||||||||
7564R-3.in silico 481 GCCCGCATCCACCT<span class="snp"><tt>-</tt></span>ACCAGCAGCA 505
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140734.1 |
CG7564-RA (CG7564), mRNA |
| 0.2 |
1 |
4 |
6 |
11 |
NM_167846.1 |
CG1086-RA, transcript variant A (Glut1), mRNA |
| 0.2 |
1 |
2 |
4 |
4 |
NM_167281.1 |
CG1725-RF, transcript variant F (dlg1), mRNA |
| 0 |
0 |
3 |
7 |
10 |
NM_137943.2 |
CG3493-RA (CG3493), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_057840.3 |
CG2488-RB, transcript variant B (phr6-4), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_165334.1 |
CG2488-RA, transcript variant A (phr6-4), mRNA |
| 0 |
0 |
2 |
5 |
7 |
NM_144358.2 |
CG14233-RA (meso18E), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_080523.2 |
CG9858-RA (clt), mRNA |
| 0 |
0 |
2 |
3 |
10 |
NM_170572.1 |
CG1910-RC, transcript variant C (CG1910), mRNA |
| 0 |
0 |
2 |
3 |
10 |
NM_170574.1 |
CG1910-RD, transcript variant D (CG1910), mRNA |
| 0 |
0 |
2 |
3 |
10 |
NM_143631.2 |
CG1910-RA, transcript variant A (CG1910), mRNA |
| 0 |
0 |
2 |
3 |
10 |
NM_170573.2 |
CG1910-RB, transcript variant B (CG1910), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_166035.1 |
CG8367-RA, transcript variant A (cg), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_166036.1 |
CG8367-RC, transcript variant C (cg), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_079014.2 |
CG8367-RD, transcript variant D (cg), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_166034.1 |
CG8367-RB, transcript variant B (cg), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_168009.1 |
CG14959-RB, transcript variant B (CG14959), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206264.1 |
CG14959-RC, transcript variant C (CG14959), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_139539.1 |
CG14959-RA, transcript variant A (CG14959), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_137133.2 |
CG10143-RA (Adgf-E), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_139492.2 |
CG2103-RA, transcript variant A (pgant6), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_167966.1 |
CG2103-RB, transcript variant B (pgant6), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_142738.2 |
CG6560-RA (CG6560), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167072.1 |
CG3861-RB, transcript variant B (l(1)G0030), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132091.2 |
CG3861-RA, transcript variant A (l(1)G0030), mRNA |
| 0 |
0 |
2 |
1 |
2 |
NM_167543.1 |
CG32574-RA (CG32574), mRNA |
| 0 |
0 |
1 |
10 |
9 |
NM_057391.3 |
CG1977-RA (alpha-Spec), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_080138.2 |
CG1618-RA (comt), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_080142.2 |
CG1925-RA (mus205), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_057618.2 |
CG3399-RA, transcript variant A (capu), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| + |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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|
