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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7560R-3 
 Symbol CG7560  Full Name CG7560 
 CG No CG7560  Old CG No CG7560 
 Synonyms gh18008, BEST:GH18008, CG7560 
 Accession No (Link to NCBI) NM_140205.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGCCTCCTACATTCCCTGCGT 
 Primer Seq. 3' 
aatctagaggtaccCTCTGGATATCCACCCACACA 
 Predicted Fragment Size 
499 
 IR fragment full Seq 
0001 TGCCTCNCTA CATTCCCTGC GTGACCTTTG CCCCCGATCA AAATGAATCC GTAGTGACGA 
0061 AGCAGCGGAT AGGAACGCTC GTGGCGGAGC GAACAGCCCA ACATNCGCCT CTTCTACGGC 
0121 ATCGAGGTGA TGGCCAGCGA TCCGAGTGGC CGGCCAACCT GCCTGGACTT TAATCACTTC 
0181 CTGCNCGCTC CTGCCCACCT TCGTGAGCTT CGTTTGGCTG AGTCAGCGGT ACTGGGACGT 
0241 GGAGCCAGTG GGTCAGGTGG AGAGCCTTCA GATGGCCCAA CACCTGGCCA CACGCATTCC 
0301 CGTGCTGCCC CATGTGTCCG CCTATCGAAT GAGTAGGCAG CGACTGGATC AGTTTTTGGC 
0361 CCTCAACTTT AGCAGTCTTT TGGCCGTTCG AGGGGATCGA GTTCACGAGG ATCAGGATTT 
0421 CTCGATTAGC TACCCCATGG TGGAGCAATC GAAACGCCAG AGGGGTGAAA CAATATCAGT 
0481 CTGTGTGGGT GGATATCCAG AG 
 in silico PCR Fragment 
0001 TGCCTC-CTA CATTCCCTGC GTGACCTTTG CCCCCGATCA AAATGAATCC GTAGTGACGA 
0061 AGCAGCGGAT AGGAACGCTC GTGGCGGAGC GAACAGCCCA ACAT-CGCCT CTTCTACGGC 
0121 ATCGAGGTGA TGGCCAGCGA TCCGAGTGGC CGGCCAACCT GCCTGGACTT TAATCACTTC 
0181 CTGC-CGCTC CTGCCCACCT TCGTGAGCTT CGTTTGGCTG AGTCAGCGGT ACTGGGACGT 
0241 GGAGCCAGTG GGTCAGGTGG AGAGCCTTCA GATGGCCCAA CACCTGGCCA CACGCATTCC 
0301 CGTGCTGCCC CATGTGTCCG CCTATCGAAT GAGTAGGCAG CGACTGGATC AGTTTTTGGC 
0361 CCTCAACTTT AGCAGTCTTT TGGCCGTTCG AGGGGATCGA GTTCACGAGG ATCAGGATTT 
0421 CTCGATTAGC TACCCCATGG TGGAGCAATC GATACGGCAG AGGGGTGAAA CAATATCAGT 
0481 CTGTGTGGGT GGATATCCAG AG 
 Assemble Data 
<PRE>

7560R-3.IR_full       1   TGCCTC<span class="snp"><tt>N</tt></span>CTACATTCCCTGCGTGACCTTTGCCCCCGATCAAAATGAATCCGTAGTGACGA 60
                          ||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-3.in silico     1   TGCCTC<span class="snp"><tt>-</tt></span>CTACATTCCCTGCGTGACCTTTGCCCCCGATCAAAATGAATCCGTAGTGACGA 60


7560R-3.IR_full       61  AGCAGCGGATAGGAACGCTCGTGGCGGAGCGAACAGCCCAACAT<span class="snp"><tt>N</tt></span>CGCCTCTTCTACGGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||
7560R-3.in silico     61  AGCAGCGGATAGGAACGCTCGTGGCGGAGCGAACAGCCCAACAT<span class="snp"><tt>-</tt></span>CGCCTCTTCTACGGC 120


7560R-3.IR_full       121 ATCGAGGTGATGGCCAGCGATCCGAGTGGCCGGCCAACCTGCCTGGACTTTAATCACTTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-3.in silico     121 ATCGAGGTGATGGCCAGCGATCCGAGTGGCCGGCCAACCTGCCTGGACTTTAATCACTTC 180


7560R-3.IR_full       181 CTGC<span class="snp"><tt>N</tt></span>CGCTCCTGCCCACCTTCGTGAGCTTCGTTTGGCTGAGTCAGCGGTACTGGGACGT 240
                          ||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-3.in silico     181 CTGC<span class="snp"><tt>-</tt></span>CGCTCCTGCCCACCTTCGTGAGCTTCGTTTGGCTGAGTCAGCGGTACTGGGACGT 240


7560R-3.IR_full       241 GGAGCCAGTGGGTCAGGTGGAGAGCCTTCAGATGGCCCAACACCTGGCCACACGCATTCC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-3.in silico     241 GGAGCCAGTGGGTCAGGTGGAGAGCCTTCAGATGGCCCAACACCTGGCCACACGCATTCC 300


7560R-3.IR_full       301 CGTGCTGCCCCATGTGTCCGCCTATCGAATGAGTAGGCAGCGACTGGATCAGTTTTTGGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-3.in silico     301 CGTGCTGCCCCATGTGTCCGCCTATCGAATGAGTAGGCAGCGACTGGATCAGTTTTTGGC 360


7560R-3.IR_full       361 CCTCAACTTTAGCAGTCTTTTGGCCGTTCGAGGGGATCGAGTTCACGAGGATCAGGATTT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-3.in silico     361 CCTCAACTTTAGCAGTCTTTTGGCCGTTCGAGGGGATCGAGTTCACGAGGATCAGGATTT 420


7560R-3.IR_full       421 CTCGATTAGCTACCCCATGGTGGAGCAATCGA<span class="snp"><tt>A</tt></span>ACG<span class="snp"><tt>C</tt></span>CAGAGGGGTGAAACAATATCAGT 480
                          ||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
7560R-3.in silico     421 CTCGATTAGCTACCCCATGGTGGAGCAATCGA<span class="snp"><tt>T</tt></span>ACG<span class="snp"><tt>G</tt></span>CAGAGGGGTGAAACAATATCAGT 480


7560R-3.IR_full       481 CTGTGTGGGTGGATATCCAGAG 502
                          ||||||||||||||||||||||
7560R-3.in silico     481 CTGTGTGGGTGGATATCCAGAG 502

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   481  0  0  0  NM_140205.1  CG7560-RA (CG7560), mRNA 
0   0  2  2  2  NM_079876.2  CG1483-RA, transcript variant A (Map205), mRNA 
0   0  2  2  2  NM_170586.1  CG1483-RB, transcript variant B (Map205), mRNA 
0   0  2  2  2  NM_136386.2  CG3409-RA (CG3409), mRNA 
0   0  1  4  6  NM_135339.2  CG12375-RA (CG12375), mRNA 
0   0  1  4  2  NM_167481.2  CG9209-RA, transcript variant A (vap), mRNA 
0   0  1  4  2  NM_078637.2  CG9209-RB, transcript variant B (vap), mRNA 
0   0  1  4  2  NM_206759.1  CG9209-RD, transcript variant D (vap), mRNA 
0   0  1  4  2  NM_167480.1  CG9209-RC, transcript variant C (vap), mRNA 
0   0  1  3  9  NM_078977.2  CG10897-RA, transcript variant A (tou), mRNA 
0   0  1  3  4  NM_134770.1  CG17657-RA (CG17657), mRNA 
0   0  1  3  3  NM_001031867.1  CG33950-RF, transcript variant F (trol), mRNA 
0   0  1  3  3  NM_001031862.1  CG33950-RC, transcript variant C (trol), mRNA 
0   0  1  3  3  NM_001031864.1  CG33950-RE, transcript variant E (trol), mRNA 
0   0  1  3  3  NM_001031866.1  CG33950-RA, transcript variant A (trol), mRNA 
0   0  1  3  3  NM_001031863.1  CG33950-RD, transcript variant D (trol), mRNA 
0   0  1  3  3  NM_001031865.1  CG33950-RB, transcript variant B (trol), mRNA 
0   0  1  2  8  NM_001031931.1  CG33791-RD, transcript variant D (CG33791), mRNA 
0   0  1  2  8  NM_001031929.1  CG33791-RB, transcript variant B (CG33791), mRNA 
0   0  1  2  8  NM_001031928.1  CG33791-RC, transcript variant C (CG33791), mRNA 
0   0  1  2  8  NM_001031930.1  CG33791-RA, transcript variant A (CG33791), mRNA 
0   0  1  2  8  NM_001031926.1  CG18170-RB, transcript variant B (CG18170), mRNA 
0   0  1  2  8  NM_001031927.1  CG18170-RA, transcript variant A (CG18170), mRNA 
0   0  1  2  7  NM_143745.2  CG18389-RA (Eip93F), mRNA 
0   0  1  2  2  NM_132821.1  CG8117-RA (CG8117), mRNA 
0   0  1  2  2  NM_136973.1  CG8767-RA (mos), mRNA 
0   0  1  2  2  NM_079143.2  CG3340-RA (Kr), mRNA 
0   0  0  8  4  NM_165760.2  CG18408-RB, transcript variant B (CAP), mRNA 
0   0  0  5  18  NM_175960.3  CG33196-RB (dp), mRNA 
0   0  0  5  9  NM_057638.3  CG14472-RA (poe), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme00670 : One carbon pool by folate
dme01100 : Metabolic pathways

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