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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7560R-1 |
| Symbol |
CG7560 |
Full Name |
CG7560 |
| CG No |
CG7560 |
Old CG No |
CG7560 |
| Synonyms |
gh18008, BEST:GH18008, CG7560 |
| Accession No (Link to NCBI) |
NM_140205.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGCCTCCTACATTCCCTGCGT |
| Primer Seq. 3' |
aatctagaggtaccCTCTGGATATCCACCCACACA |
| Predicted Fragment Size |
499 |
| IR fragment full Seq |
0001 TGCCTCNCTA CATTCCCTGC GTGACCTTTG CCCCCGATCA AAATGAATCC GTAGTGACGA
0061 AGCAGCGGAT AGGAACGCTC GTGGCGGAGC GAACAGCCCA ACATNCGCCT CTTCTACGGC
0121 ATCGAGGTGA TGGCCAGCGA TCCGAGTGGC CGGCCAACCT GCCTGGACTT TAATCACTTC
0181 CTGCNCGCTC CTGCCCACCT TCGTGAGCTT CGTTTGGCTG AGTCAGCGGT ACTGGGACGT
0241 GGAGCCAGTG GGTCAGGTGG AGAGCCTTCA GATGGCCCAA CACCTGGCCA CACGCATTCC
0301 CGTGCTGCCC CATGTGTCCG CCTATCGAAT GAGTAGGCAG CGACTGGATC AGTTTTTGGC
0361 CCTCAACTTT AGCAGTCTTT TGGCCGTTCG AGGGGATCGA GTTCACGAGG ATCAGGATTT
0421 CTCGATTAGC TACCCCATGG TGGAGCAATC GAAACGCCAG AGGGGTGAAA CAATATCAGT
0481 CTGTGTGGGT GGATATCCAG AG |
| in silico PCR Fragment |
0001 TGCCTC-CTA CATTCCCTGC GTGACCTTTG CCCCCGATCA AAATGAATCC GTAGTGACGA
0061 AGCAGCGGAT AGGAACGCTC GTGGCGGAGC GAACAGCCCA ACAT-CGCCT CTTCTACGGC
0121 ATCGAGGTGA TGGCCAGCGA TCCGAGTGGC CGGCCAACCT GCCTGGACTT TAATCACTTC
0181 CTGC-CGCTC CTGCCCACCT TCGTGAGCTT CGTTTGGCTG AGTCAGCGGT ACTGGGACGT
0241 GGAGCCAGTG GGTCAGGTGG AGAGCCTTCA GATGGCCCAA CACCTGGCCA CACGCATTCC
0301 CGTGCTGCCC CATGTGTCCG CCTATCGAAT GAGTAGGCAG CGACTGGATC AGTTTTTGGC
0361 CCTCAACTTT AGCAGTCTTT TGGCCGTTCG AGGGGATCGA GTTCACGAGG ATCAGGATTT
0421 CTCGATTAGC TACCCCATGG TGGAGCAATC GATACGGCAG AGGGGTGAAA CAATATCAGT
0481 CTGTGTGGGT GGATATCCAG AG |
| Assemble Data |
<PRE>
7560R-1.IR_full 1 TGCCTC<span class="snp"><tt>N</tt></span>CTACATTCCCTGCGTGACCTTTGCCCCCGATCAAAATGAATCCGTAGTGACGA 60
||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-1.in silico 1 TGCCTC<span class="snp"><tt>-</tt></span>CTACATTCCCTGCGTGACCTTTGCCCCCGATCAAAATGAATCCGTAGTGACGA 60
7560R-1.IR_full 61 AGCAGCGGATAGGAACGCTCGTGGCGGAGCGAACAGCCCAACAT<span class="snp"><tt>N</tt></span>CGCCTCTTCTACGGC 120
||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||
7560R-1.in silico 61 AGCAGCGGATAGGAACGCTCGTGGCGGAGCGAACAGCCCAACAT<span class="snp"><tt>-</tt></span>CGCCTCTTCTACGGC 120
7560R-1.IR_full 121 ATCGAGGTGATGGCCAGCGATCCGAGTGGCCGGCCAACCTGCCTGGACTTTAATCACTTC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-1.in silico 121 ATCGAGGTGATGGCCAGCGATCCGAGTGGCCGGCCAACCTGCCTGGACTTTAATCACTTC 180
7560R-1.IR_full 181 CTGC<span class="snp"><tt>N</tt></span>CGCTCCTGCCCACCTTCGTGAGCTTCGTTTGGCTGAGTCAGCGGTACTGGGACGT 240
||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-1.in silico 181 CTGC<span class="snp"><tt>-</tt></span>CGCTCCTGCCCACCTTCGTGAGCTTCGTTTGGCTGAGTCAGCGGTACTGGGACGT 240
7560R-1.IR_full 241 GGAGCCAGTGGGTCAGGTGGAGAGCCTTCAGATGGCCCAACACCTGGCCACACGCATTCC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-1.in silico 241 GGAGCCAGTGGGTCAGGTGGAGAGCCTTCAGATGGCCCAACACCTGGCCACACGCATTCC 300
7560R-1.IR_full 301 CGTGCTGCCCCATGTGTCCGCCTATCGAATGAGTAGGCAGCGACTGGATCAGTTTTTGGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-1.in silico 301 CGTGCTGCCCCATGTGTCCGCCTATCGAATGAGTAGGCAGCGACTGGATCAGTTTTTGGC 360
7560R-1.IR_full 361 CCTCAACTTTAGCAGTCTTTTGGCCGTTCGAGGGGATCGAGTTCACGAGGATCAGGATTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7560R-1.in silico 361 CCTCAACTTTAGCAGTCTTTTGGCCGTTCGAGGGGATCGAGTTCACGAGGATCAGGATTT 420
7560R-1.IR_full 421 CTCGATTAGCTACCCCATGGTGGAGCAATCGA<span class="snp"><tt>A</tt></span>ACG<span class="snp"><tt>C</tt></span>CAGAGGGGTGAAACAATATCAGT 480
||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||
7560R-1.in silico 421 CTCGATTAGCTACCCCATGGTGGAGCAATCGA<span class="snp"><tt>T</tt></span>ACG<span class="snp"><tt>G</tt></span>CAGAGGGGTGAAACAATATCAGT 480
7560R-1.IR_full 481 CTGTGTGGGTGGATATCCAGAG 502
||||||||||||||||||||||
7560R-1.in silico 481 CTGTGTGGGTGGATATCCAGAG 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
481 |
0 |
0 |
0 |
NM_140205.1 |
CG7560-RA (CG7560), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_079876.2 |
CG1483-RA, transcript variant A (Map205), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_170586.1 |
CG1483-RB, transcript variant B (Map205), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_136386.2 |
CG3409-RA (CG3409), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_135339.2 |
CG12375-RA (CG12375), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167481.2 |
CG9209-RA, transcript variant A (vap), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_078637.2 |
CG9209-RB, transcript variant B (vap), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_206759.1 |
CG9209-RD, transcript variant D (vap), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167480.1 |
CG9209-RC, transcript variant C (vap), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_078977.2 |
CG10897-RA, transcript variant A (tou), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_134770.1 |
CG17657-RA (CG17657), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001031867.1 |
CG33950-RF, transcript variant F (trol), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001031862.1 |
CG33950-RC, transcript variant C (trol), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001031864.1 |
CG33950-RE, transcript variant E (trol), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001031866.1 |
CG33950-RA, transcript variant A (trol), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001031863.1 |
CG33950-RD, transcript variant D (trol), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001031865.1 |
CG33950-RB, transcript variant B (trol), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001031931.1 |
CG33791-RD, transcript variant D (CG33791), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001031929.1 |
CG33791-RB, transcript variant B (CG33791), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001031928.1 |
CG33791-RC, transcript variant C (CG33791), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001031930.1 |
CG33791-RA, transcript variant A (CG33791), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001031926.1 |
CG18170-RB, transcript variant B (CG18170), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001031927.1 |
CG18170-RA, transcript variant A (CG18170), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_143745.2 |
CG18389-RA (Eip93F), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132821.1 |
CG8117-RA (CG8117), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136973.1 |
CG8767-RA (mos), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079143.2 |
CG3340-RA (Kr), mRNA |
| 0 |
0 |
0 |
8 |
4 |
NM_165760.2 |
CG18408-RB, transcript variant B (CAP), mRNA |
| 0 |
0 |
0 |
5 |
18 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_057638.3 |
CG14472-RA (poe), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| +- |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| ++ |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme00670
:
One carbon pool by folate
dme01100
:
Metabolic pathways
|
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