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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7555R-1 
 Symbol Nedd4  Full Name Nedd4 
 CG No CG7555  Old CG No CG7555 
 Synonyms nedd4, DNedd4, CG7555, dNedd4-1, dNedd4, Nedd4 
 Accession No (Link to NCBI) NM_140736.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.<br> In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.<br> PLoS One (2011) 6(9) e24168 [ PubMed ID = 21931660 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGTCGTCTTCTTCCTCGGTGG 
 Primer Seq. 3' 
aatctagaggtaccCGGGCGCAGAGTGTAGCTTTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGTCGTCTTC TTCCTCGGTG GCCGGCGgcG ACGTGCCACG CCCGCCGcCC CGCCGgcGAG 
0061 CGGCCTCTGT TGCGGGACAA CAGCAAACGC GCCAGGAATT TGGAAATGGT TACACTCCTC 
0121 GCAGATCGTT GGCAGCAGTG AACGACTCTG GGGACTCGTG TCACCTGCGA ATCGTCGTAC 
0181 TCACCGGCCA GTCGCTGGCC AAAAAGGATA TATTCGGCGC CAGCGACNCC GTATGTCAGA 
0241 ATCGATTTGA ACACAATCAA CGGTGATATT AATATTGACT CTGTTTTGAC GAAGACCAAA 
0301 AAGAAGACAC TGAATCCAAC CTGGAATGAA GAGTTCATAT TTAGAGTTAA ACCGTCTGAG 
0361 CATAAGCTTG TGTTTCAAGT ATTTGACGAG AATCGCCTGA CACGCGACGA CTTCCTGGGC 
0421 ATGGTGGAGC TGACCCTGGT GAATCTGCCT ACCGAGCAGG AAGGTCGCAC CATCGGAGAA 
0481 CAAAGCTACA NCTCTGCgCC CG 
 in silico PCR Fragment 
0001 CGTCGTCTTC TTCCTCGGTG GCCGGCGGCG ACGTGCCACG CCCGCCGCCC CGCCGGCGAG 
0061 CGGCCTCTGT TGCGGGACAA CAGCAAACGC GCCAGGAATT TGGAAATGGT TACACTCCTC 
0121 GCAGATCGTT GGCAGCAGTG AACGACTCTG GGGACTCGTG TCACCTGCGA ATCGTCGTAC 
0181 TCACCGGCCA GTCGCTGGCC AAAAAGGATA TATTCGGCGC CAGCGAC-CC GTATGTCAGA 
0241 ATCGATTTGA ACACAATCAA CGGTGATATT AATATTGACT CTGTTTTGAC GAAGACCAAA 
0301 AAGAAGACAC TGAATCCAAC CTGGAATGAA GAGTTCATAT TTAGAGTTAA ACCGTCTGAG 
0361 CATAAGCTTG TGTTTCAAGT ATTTGACGAG AATCGCCTGA CACGCGACGA CTTCCTGGGC 
0421 ATGGTGGAGC TGACCCTGGT GAATCTGCCT ACCGAGCAGG AAGGTCGCAC CATCGGAGAA 
0481 CAAAGCTACA -CTCTGCGCC CG 
 Assemble Data 
<PRE>

7555R-1.IR_full       1   CGTCGTCTTCTTCCTCGGTGGCCGGCGGCGACGTGCCACGCCCGCCGCCCCGCCGGCGAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7555R-1.in silico     1   CGTCGTCTTCTTCCTCGGTGGCCGGCGGCGACGTGCCACGCCCGCCGCCCCGCCGGCGAG 60


7555R-1.IR_full       61  CGGCCTCTGTTGCGGGACAACAGCAAACGCGCCAGGAATTTGGAAATGGTTACACTCCTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7555R-1.in silico     61  CGGCCTCTGTTGCGGGACAACAGCAAACGCGCCAGGAATTTGGAAATGGTTACACTCCTC 120


7555R-1.IR_full       121 GCAGATCGTTGGCAGCAGTGAACGACTCTGGGGACTCGTGTCACCTGCGAATCGTCGTAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7555R-1.in silico     121 GCAGATCGTTGGCAGCAGTGAACGACTCTGGGGACTCGTGTCACCTGCGAATCGTCGTAC 180


7555R-1.IR_full       181 TCACCGGCCAGTCGCTGGCCAAAAAGGATATATTCGGCGCCAGCGAC<span class="snp"><tt>N</tt></span>CCGTATGTCAGA 240
                          |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
7555R-1.in silico     181 TCACCGGCCAGTCGCTGGCCAAAAAGGATATATTCGGCGCCAGCGAC<span class="snp"><tt>-</tt></span>CCGTATGTCAGA 240


7555R-1.IR_full       241 ATCGATTTGAACACAATCAACGGTGATATTAATATTGACTCTGTTTTGACGAAGACCAAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7555R-1.in silico     241 ATCGATTTGAACACAATCAACGGTGATATTAATATTGACTCTGTTTTGACGAAGACCAAA 300


7555R-1.IR_full       301 AAGAAGACACTGAATCCAACCTGGAATGAAGAGTTCATATTTAGAGTTAAACCGTCTGAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7555R-1.in silico     301 AAGAAGACACTGAATCCAACCTGGAATGAAGAGTTCATATTTAGAGTTAAACCGTCTGAG 360


7555R-1.IR_full       361 CATAAGCTTGTGTTTCAAGTATTTGACGAGAATCGCCTGACACGCGACGACTTCCTGGGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7555R-1.in silico     361 CATAAGCTTGTGTTTCAAGTATTTGACGAGAATCGCCTGACACGCGACGACTTCCTGGGC 420


7555R-1.IR_full       421 ATGGTGGAGCTGACCCTGGTGAATCTGCCTACCGAGCAGGAAGGTCGCACCATCGGAGAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7555R-1.in silico     421 ATGGTGGAGCTGACCCTGGTGAATCTGCCTACCGAGCAGGAAGGTCGCACCATCGGAGAA 480


7555R-1.IR_full       481 CAAAGCTACA<span class="snp"><tt>N</tt></span>CTCTGCGCCCG 502
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||
7555R-1.in silico     481 CAAAGCTACA<span class="snp"><tt>-</tt></span>CTCTGCGCCCG 502

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140736.2  CG7555-RA, transcript variant A (Nedd4), mRNA 
100   482  0  0  0  NM_168736.1  CG7555-RC, transcript variant C (Nedd4), mRNA 
70.95   342  1  2  1  NM_206394.1  CG7555-RD, transcript variant D (Nedd4), mRNA 
70.95   342  1  2  1  NM_168737.1  CG7555-RB, transcript variant B (Nedd4), mRNA 
0   0  3  5  3  NM_136259.2  CG8671-RB, transcript variant B (CG8671), mRNA 
0   0  3  3  3  NM_133003.2  CG32555-RB, transcript variant B (RhoGAPp190), mRNA 
0   0  3  3  3  NM_167573.1  CG32555-RC, transcript variant C (RhoGAPp190), mRNA 
0   0  3  3  3  NM_167572.1  CG32555-RA, transcript variant A (RhoGAPp190), mRNA 
0   0  3  3  2  NM_141856.2  CG6930-RA, transcript variant A (l(3)neo38), mRNA 
0   0  3  3  2  NM_001043244.1  CG6930-RC, transcript variant C (l(3)neo38), mRNA 
0   0  3  3  2  NM_001043243.1  CG6930-RB, transcript variant B (l(3)neo38), mRNA 
0   0  3  2  4  NM_135978.1  CG12288-RA (CG12288), mRNA 
0   0  2  2  3  NM_079211.2  CG10583-RA (Sse), mRNA 
0   0  2  2  3  NM_137412.2  CG6406-RB, transcript variant B (CG6406), mRNA 
0   0  2  2  3  NM_166247.1  CG6406-RA, transcript variant A (CG6406), mRNA 
0   0  1  4  5  NM_132779.1  CG15028-RB, transcript variant B (CG15028), mRNA 
0   0  1  4  5  NM_167431.1  CG15028-RA, transcript variant A (CG15028), mRNA 
0   0  1  4  3  NM_079719.2  CG6575-RA (glec), mRNA 
0   0  1  4  2  NM_132989.2  CG8568-RA (CG8568), mRNA 
0   0  1  4  2  NM_176244.1  CG9696-RE, transcript variant E (dom), mRNA 
0   0  1  4  2  NM_166446.1  CG9696-RD, transcript variant D (dom), mRNA 
0   0  1  4  2  NM_080094.2  CG9696-RA, transcript variant A (dom), mRNA 
0   0  1  3  2  NM_169036.1  CG31543-RC, transcript variant C (Hph), mRNA 
0   0  1  3  2  NM_141268.2  CG31543-RA, transcript variant A (Hph), mRNA 
0   0  1  3  2  NM_169037.1  CG31543-RB, transcript variant B (Hph), mRNA 
0   0  1  3  2  NM_057840.3  CG2488-RB, transcript variant B (phr6-4), mRNA 
0   0  1  3  2  NM_165334.1  CG2488-RA, transcript variant A (phr6-4), mRNA 
0   0  1  3  2  NM_130580.1  CG14797-RA (CG14797), mRNA 
0   0  1  2  2  NM_141128.2  CG9063-RA (CG9063), mRNA 
0   0  0  7  6  NM_164688.1  CG31640-RA (CG31640), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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