NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7542R-1

Symbol

CG7542

Full Name

CG7542

CG No

CG7542

Old CG No

CG7542

Synonyms

SP71, CG7542

Accession No (Link to NCBI)

NM_140738.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.<br> Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.<br> Curr Biol (2006) 16(8) 808-13 [ PubMed ID = 16631589 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTACGGCTGTTCCCCTCCTTA

Primer Seq. 3'

aatctagaggtaccCATCTCCACGTATCTCAGCAC

Predicted Fragment Size

IR fragment full Seq

0001 GTACGGCTGT TCCCCTCCTT ACCGATGTGG AACCCTATAT TACGAATGGA GAACCGGCGG 
0061 AGGTGGGTCA GTTTCCCTAC CAGGCCGGTC TGAACGTCTC CTTCGGGAAC TGGAGCACTT 
0121 GGTGCGGCGG CACCTTGNAT CTCACACTAC TGGATAATCA CAGCAGCACA CTGCATGGAT 
0181 GGGGCGGAGT NCGTAACGGT CTATTTGGGG GCCATAAACA TCGGTGATGA GTCCGAGGAG 
0241 GGTCAGGAAA GGATCATGGT GGAGAAGTCG GGTATTATAG TGCACTCGAA CTATATGGCC 
0301 AGCACGGTGG TCAACGACAT CTCGCTTATT CGATTGCCCG CCTTTGTGGG TTTCACTGAT 
0361 CGCATCCGGG CCGCCAGTTT GCCCCGTCGC TTGAATGGCC AGTTTCCCAC GTACGAGTCC 
0421 ATCCGGGCCT TCGCCTCCGG CTGGGGGCGG GAAAGCGATG CCTCCGACTC GGTTTCCCCC 
0481 GTGCTGAGAT ACGTGGAGAT G

in silico PCR Fragment

0001 GTACGGCTGT TCCCCTCCTT ACCGATGTGG AACCCTATAT TACGAATGGA GAACCGGCGG 
0061 AGGTGGGTCA GTTTCCCTAC CAGGCCGGTC TGAACGTCTC CTTCGGGAAC TGGAGCACTT 
0121 GGTGCGGCGG CACCTTG-AT CTCACACTAC TGGATAATCA CAGCAGCACA CTGCATGGAT 
0181 GGGGCGGAGT CCGTAACGGT CTATTTGGGG GCCATAAACA TCGGTGATGA GTCCGAGGAG 
0241 GGTCAAGAAA GGATCATGGT GGAGAAGTCG GGTATTATAG TGCACTCGAA CTATATGGCC 
0301 AGCACGGTGG TCAACGACAT CTCGCTTATT CGATTGCCCG CCTTTGTGGG TTTCACTGAT 
0361 CGCATCCGGG CCGCCAGTTT GCCCCGTCGC TTGAATGGCC AGTTTCCCAC GTACGAGTCC 
0421 ATCCGGGCCT TCGCCTCCGG CTGGGGGCGG GAAAGCGATG CCTCCGACTC GGTTTCCCCC 
0481 GTGCTGAGAT ACGTGGAGAT G

Assemble Data

<PRE>

7542R-1.IR_full       1   GTACGGCTGTTCCCCTCCTTACCGATGTGGAACCCTATATTACGAATGGAGAACCGGCGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7542R-1.in silico     1   GTACGGCTGTTCCCCTCCTTACCGATGTGGAACCCTATATTACGAATGGAGAACCGGCGG 60


7542R-1.IR_full       61  AGGTGGGTCAGTTTCCCTACCAGGCCGGTCTGAACGTCTCCTTCGGGAACTGGAGCACTT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7542R-1.in silico     61  AGGTGGGTCAGTTTCCCTACCAGGCCGGTCTGAACGTCTCCTTCGGGAACTGGAGCACTT 120


7542R-1.IR_full       121 GGTGCGGCGGCACCTTG<span class="snp"><tt>N</tt></span>ATCTCACACTACTGGATAATCACAGCAGCACACTGCATGGAT 180
                          |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||
7542R-1.in silico     121 GGTGCGGCGGCACCTTG<span class="snp"><tt>-</tt></span>ATCTCACACTACTGGATAATCACAGCAGCACACTGCATGGAT 180


7542R-1.IR_full       181 GGGGCGGAGT<span class="snp"><tt>N</tt></span>CGTAACGGTCTATTTGGGGGCCATAAACATCGGTGATGAGTCCGAGGAG 240
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
7542R-1.in silico     181 GGGGCGGAGT<span class="snp"><tt>C</tt></span>CGTAACGGTCTATTTGGGGGCCATAAACATCGGTGATGAGTCCGAGGAG 240


7542R-1.IR_full       241 GGTCA<span class="snp"><tt>G</tt></span>GAAAGGATCATGGTGGAGAAGTCGGGTATTATAGTGCACTCGAACTATATGGCC 300
                          |||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||
7542R-1.in silico     241 GGTCA<span class="snp"><tt>A</tt></span>GAAAGGATCATGGTGGAGAAGTCGGGTATTATAGTGCACTCGAACTATATGGCC 300


7542R-1.IR_full       301 AGCACGGTGGTCAACGACATCTCGCTTATTCGATTGCCCGCCTTTGTGGGTTTCACTGAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7542R-1.in silico     301 AGCACGGTGGTCAACGACATCTCGCTTATTCGATTGCCCGCCTTTGTGGGTTTCACTGAT 360


7542R-1.IR_full       361 CGCATCCGGGCCGCCAGTTTGCCCCGTCGCTTGAATGGCCAGTTTCCCACGTACGAGTCC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7542R-1.in silico     361 CGCATCCGGGCCGCCAGTTTGCCCCGTCGCTTGAATGGCCAGTTTCCCACGTACGAGTCC 420


7542R-1.IR_full       421 ATCCGGGCCTTCGCCTCCGGCTGGGGGCGGGAAAGCGATGCCTCCGACTCGGTTTCCCCC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7542R-1.in silico     421 ATCCGGGCCTTCGCCTCCGGCTGGGGGCGGGAAAGCGATGCCTCCGACTCGGTTTCCCCC 480


7542R-1.IR_full       481 GTGCTGAGATACGTGGAGATG 501
                          |||||||||||||||||||||
7542R-1.in silico     481 GTGCTGAGATACGTGGAGATG 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140738.2	CG7542-RA (CG7542), mRNA
0	0	2	3	2	NM_206141.1	CG33456-RA (CG33456), mRNA
0	0	1	4	2	NM_136390.2	CG3420-RA (CG3420), mRNA
0	0	1	4	2	NM_140155.2	CG7958-RA, transcript variant A (tna), mRNA
0	0	1	4	2	NM_168422.1	CG7958-RB, transcript variant B (tna), mRNA
0	0	1	3	4	NM_165370.1	CG31622-RB, transcript variant B (Gr39a), mRNA
0	0	1	2	2	NM_078924.3	CG12165-RA (Incenp), mRNA
0	0	0	5	4	NM_137570.1	CG10051-RA (CG10051), mRNA
0	0	0	5	3	NM_142694.1	CG10824-RA (CG10824), mRNA
0	0	0	4	7	NM_206163.1	CG5170-RB, transcript variant B (Dp1), mRNA
0	0	0	4	7	NM_079057.2	CG5170-RC, transcript variant C (Dp1), mRNA
0	0	0	4	7	NM_206164.1	CG5170-RA, transcript variant A (Dp1), mRNA
0	0	0	4	7	NM_166280.1	CG5170-RD, transcript variant D (Dp1), mRNA
0	0	0	4	7	NM_166281.1	CG5170-RE, transcript variant E (Dp1), mRNA
0	0	0	4	7	NM_166282.1	CG5170-RF, transcript variant F (Dp1), mRNA
0	0	0	4	6	NM_167053.1	CG4027-RA, transcript variant A (Act5C), mRNA
0	0	0	4	6	NM_078497.2	CG4027-RB, transcript variant B (Act5C), mRNA
0	0	0	4	6	NM_001014726.1	CG4027-RC, transcript variant C (Act5C), mRNA
0	0	0	4	6	NM_206801.1	CG1391-RD, transcript variant D (sol), mRNA
0	0	0	4	6	NM_001014725.1	CG4027-RD, transcript variant D (Act5C), mRNA
0	0	0	4	5	NM_057390.4	CG1391-RB, transcript variant B (sol), mRNA
0	0	0	4	5	NM_079643.1	CG5178-RA (Act88F), mRNA
0	0	0	4	4	NM_080292.2	CG3972-RA (Cyp4g1), mRNA
0	0	0	4	4	NM_165223.1	CG6433-RB, transcript variant B (qua), mRNA
0	0	0	4	4	NM_079117.2	CG3385-RA (nvy), mRNA
0	0	0	4	4	NM_165222.1	CG6433-RA, transcript variant A (qua), mRNA
0	0	0	4	2	NM_206465.1	CG11992-RD, transcript variant D (Rel), mRNA
0	0	0	4	2	NM_206466.1	CG11992-RC, transcript variant C (Rel), mRNA
0	0	0	4	2	NM_206467.1	CG11992-RB, transcript variant B (Rel), mRNA
0	0	0	4	2	NM_057746.3	CG11992-RA, transcript variant A (Rel), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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