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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7509R-1 |
| Symbol |
CG7509 |
Full Name |
CG7509 |
| CG No |
CG7509 |
Old CG No |
CG7509 |
| Synonyms |
tartan/capricious-like, CT1509, CG7509 |
| Accession No (Link to NCBI) |
NM_139674.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTCCCTCTCATTTCCTCGGAC |
| Primer Seq. 3' |
aatctagaggtaccGCACGGTTAGTCCTCGAAAGG |
| Predicted Fragment Size |
490 |
| IR fragment full Seq |
0001 NTCCCTCTCA TTTCCTCGGA CACAAGAAAC TGCAGTCTGA ATGCATGTGA ATCACTGGGC
0061 ATTGTGTCCC AGCTATTGAT GCTTATCAAC TCCATGCCAA ATGGAACCCA GTCCGCTGCG
0121 AACGATAAAA AACCACCTAA GTCCAAGGCG AATGGACATA ATGATGGAGA TGGCAATGGC
0181 GGGGAGATTA ATGCCATGTC CCATTTGGCC AACTTTGATC TGGTGAAGCG GGTGCGACAG
0241 ATCGAGAGTC GACTACGATC CGTGGAGCAG CCCGTCTGGC ACTTGGNCGC TGGCAGCCAA
0301 ATCGAGTGGA ATCACTGCAC CTCAGGCGTG TGTCGCTGCA ATCCGGACAC CAAGAGCTTC
0361 ACCTGCTGGA ATACCAATCT TAAATCAGTG CCTGTCACTC AGGTGATACC CATGAATATG
0421 GTCAACATAG ATCTTTCGAG GAACATCCTT TCCACCATGC ACAAGGATAC CTTTCGAGGA
0481 CTAANCCGtg C |
| in silico PCR Fragment |
0001 CTCCCTCTCA TTTCCTCGGA CACAAGAAAC TGCAGTCTGA ATGCATGTGA ATCACTGGGT
0061 ATTGTATCCC AGCTATTGAT GCTTATCAAC TCAATGCCAA ATGGAACCCA GTCCGCTGCG
0121 AACGATAAAA AACCACCTAA GTCCAAGGCG AATGGACATA ATGATGGAGA TGTCAATGGC
0181 GGTGAGATTA ATGCCATGTC CCATTTGGCC AACTTTGATC TGGTGAAGCG GGTGCGACAG
0241 ATCGAGAGTC GACTACGATC CGTGGAGCAG CCCGTCTGGC ACTTGGCCAC TGGCAGCCAA
0301 ATCGAGTGGA ATCACTGCAC CTCAGGCGTG TGTCGCTGCA ATCCGGACAC CAAGAGCTTC
0361 ACCTGCTGGA ATACCAATCT TAAATCAGTG CCTGTCACTC AGGTGATACC CATGAATATG
0421 GTCAACATAG ATCTTTCGCG GAACATCCTT TCCACCCTGC ACAAGGATAC CTTTCGAGGA
0481 CTAA-CCGTG C |
| Assemble Data |
<PRE>
7509R-1.IR_full 1 TCCCTCTCATTTCCTCGGACACAAGAAACTGCAGTCTGAATGCATGTGAATCACTGGG<span class="snp"><tt>C</tt></span> 59
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>
7509R-1.in silico 1 TCCCTCTCATTTCCTCGGACACAAGAAACTGCAGTCTGAATGCATGTGAATCACTGGG<span class="snp"><tt>T</tt></span> 59
7509R-1.IR_full 61 ATTGT<span class="snp"><tt>G</tt></span>TCCCAGCTATTGATGCTTATCAACTC<span class="snp"><tt>C</tt></span>ATGCCAAATGGAACCCAGTCCGCTGCG 119
|||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||
7509R-1.in silico 61 ATTGT<span class="snp"><tt>A</tt></span>TCCCAGCTATTGATGCTTATCAACTC<span class="snp"><tt>A</tt></span>ATGCCAAATGGAACCCAGTCCGCTGCG 119
7509R-1.IR_full 121 AACGATAAAAAACCACCTAAGTCCAAGGCGAATGGACATAATGATGGAGATG<span class="snp"><tt>G</tt></span>CAATGGC 179
||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||
7509R-1.in silico 121 AACGATAAAAAACCACCTAAGTCCAAGGCGAATGGACATAATGATGGAGATG<span class="snp"><tt>T</tt></span>CAATGGC 179
7509R-1.IR_full 181 GG<span class="snp"><tt>G</tt></span>GAGATTAATGCCATGTCCCATTTGGCCAACTTTGATCTGGTGAAGCGGGTGCGACAG 239
||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7509R-1.in silico 181 GG<span class="snp"><tt>T</tt></span>GAGATTAATGCCATGTCCCATTTGGCCAACTTTGATCTGGTGAAGCGGGTGCGACAG 239
7509R-1.IR_full 241 ATCGAGAGTCGACTACGATCCGTGGAGCAGCCCGTCTGGCACTTGG<span class="snp"><tt>N</tt></span>C<span class="snp"><tt>G</tt></span>CTGGCAGCCAA 299
||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|<span class="snp"><tt> </tt></span>|||||||||||
7509R-1.in silico 241 ATCGAGAGTCGACTACGATCCGTGGAGCAGCCCGTCTGGCACTTGG<span class="snp"><tt>C</tt></span>C<span class="snp"><tt>A</tt></span>CTGGCAGCCAA 299
7509R-1.IR_full 301 ATCGAGTGGAATCACTGCACCTCAGGCGTGTGTCGCTGCAATCCGGACACCAAGAGCTTC 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7509R-1.in silico 301 ATCGAGTGGAATCACTGCACCTCAGGCGTGTGTCGCTGCAATCCGGACACCAAGAGCTTC 359
7509R-1.IR_full 361 ACCTGCTGGAATACCAATCTTAAATCAGTGCCTGTCACTCAGGTGATACCCATGAATATG 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7509R-1.in silico 361 ACCTGCTGGAATACCAATCTTAAATCAGTGCCTGTCACTCAGGTGATACCCATGAATATG 419
7509R-1.IR_full 421 GTCAACATAGATCTTTCG<span class="snp"><tt>A</tt></span>GGAACATCCTTTCCACC<span class="snp"><tt>A</tt></span>TGCACAAGGATACCTTTCGAGGA 479
||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||
7509R-1.in silico 421 GTCAACATAGATCTTTCG<span class="snp"><tt>C</tt></span>GGAACATCCTTTCCACC<span class="snp"><tt>C</tt></span>TGCACAAGGATACCTTTCGAGGA 479
7509R-1.IR_full 481 CTAA<span class="snp"><tt>N</tt></span>CCGTGC 490
||||<span class="snp"><tt> </tt></span>||||||
7509R-1.in silico 481 CTAA<span class="snp"><tt>-</tt></span>CCGTGC 490
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
472 |
0 |
0 |
0 |
NM_139674.2 |
CG7509-RA (CG7509), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_058130.4 |
CG5025-RA, transcript variant A (Sps2), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_058129.4 |
CG5025-RB, transcript variant B (Sps2), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_132719.2 |
CG32604-RA, transcript variant A (l(1)G0007), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_136147.2 |
CG10364-RA (msb1l), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_137854.2 |
CG4373-RA (Cyp6d2), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_079148.2 |
CG6883-RA, transcript variant A (trh), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_001043110.1 |
CG6883-RB, transcript variant B (trh), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_141005.1 |
CG3618-RA (CG3618), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_132393.2 |
CG2221-RA (l(1)G0289), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080363.5 |
CG17213-RA (Gr33a), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_136009.1 |
CG5559-RA (CG5559), mRNA |
| 0 |
0 |
1 |
8 |
5 |
NM_135248.1 |
CG13776-RA (CG13776), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_140755.1 |
CG14585-RA (CG14585), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_138005.1 |
CG13562-RA (CG13562), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166076.1 |
CG12424-RB, transcript variant B (CG12424), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137165.1 |
CG12424-RA, transcript variant A (CG12424), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166077.1 |
CG12424-RC, transcript variant C (CG12424), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206071.1 |
CG33478-RB, transcript variant B (Or46a), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140958.1 |
CG13247-RA (CG13247), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_142428.1 |
CG12321-RA (CG12321), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_206407.1 |
CG32434-RA, transcript variant A (siz), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_140136.1 |
CG12303-RA (CG12303), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079410.2 |
CG7354-RA (mRpS26), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_144487.1 |
CG5518-RA (sda), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_135791.1 |
CG15639-RA (CG15639), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_138250.2 |
CG9149-RA (CG9149), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_079452.2 |
CG7395-RA (NPFR76F), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_164890.1 |
CG4839-RB, transcript variant B (CG4839), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_135505.2 |
CG4839-RA, transcript variant A (CG4839), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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