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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7492R-1

Symbol

CG7492

Full Name

CG7492

CG No

CG7492

Old CG No

CG7492

Synonyms

anon-EST:fe1A11, CG7492

Accession No (Link to NCBI)

NM_139889.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

larval lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCGGTATACTCAACACGCGGA

Primer Seq. 3'

aatctagaggtaccGAGTTGGATGCTGCGCATGAA

Predicted Fragment Size

IR fragment full Seq

0001 CCGGTATACT CAACACGCGG AGAAGACTCG AGTGGACCAA GGAGTGGCTG CGCAAAAACC 
0061 AGACCGAGTT TCTCAGCAAG GAGAATTTAC TTAGTGAACT GCAGTCGCGC AAGGATGAGT 
0121 GCTATCACTT GAATTACTTT CTAGCCATAA CAGAGAGCCA GTTTCGGTAT CTAGTGCAAA 
0181 AGCTGGAGCC CATCATTAGC CANGTATGCT GCCGCAACGC AAGAAGAAGT CCTTCAGCGC 
0241 CTGAGGAACG ACTGGCCATA ACGCTCAAGT ATTTGGCAAC GGGTGAAGTA CATTCTTGTC 
0301 GAAACTACTG CTTCCGTGCC TCGAAATTTG TGATAAACGA AATGATTGCC AATATCTGTC 
0361 TGGGCTTCTA CGAGCACCTC AAGGACCAAT ACGTGACACT ACCAAAGACT GACGATCAGN 
0421 TGGCGCAGNG CCGCCGAAGG AGATGGAGCG TAAGCACAAC CTGCCCCACT GTGTGGGCAA 
0481 TCTGTTCATg NGCAGCATCC AACTC

in silico PCR Fragment

0001 CCGGTATACT CAACACGCGG AGAAGACTCG AGTGGACCAA GGAGTGGCTG CGCAAAAACC 
0061 AGACCGAGTT TCTCAGCAAG GAGAATTTAC TTAGTGAACT GCAGTCGCGC AAGGATGAGT 
0121 GCTATCACTT GAATTACTTT CTAGCCATAA CAGAGAGCCA GTTTCGGTAT CTAGTGCAAA 
0181 AGCTGGAGCC CATCATTAGC CA-GTATGCT -CCGCAACGC AAGAAGAAGT CCTTCAGCGC 
0241 C-GAGGAACG ACTGGCCATA ACGCTCAAGT ATTTGGCAAC GGGTGAAGTA CATTCTTGTC 
0301 GAAACTACTG CTTCCGTGCC TCGAAATTTG TGATAAACGA AATGATTGCC AATATCTGTC 
0361 TGGGCTTCTA CGAGCACCTC AAGGACCAAT ACGTGACACT ACCAAAGACT GACGATCAG- 
0421 TGGCGCAGCG CCGCCG-AGG AGATGGAGCG TAAGCACAAC CTGCCCCACT GTGTGGGCAA 
0481 TCTGTTCATG CGCAGCATCC AACTC

Assemble Data

<PRE>

7492R-1.IR_full       1   CCGGTATACTCAACACGCGGAGAAGACTCGAGTGGACCAAGGAGTGGCTGCGCAAAAACC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7492R-1.in silico     1   CCGGTATACTCAACACGCGGAGAAGACTCGAGTGGACCAAGGAGTGGCTGCGCAAAAACC 60


7492R-1.IR_full       61  AGACCGAGTTTCTCAGCAAGGAGAATTTACTTAGTGAACTGCAGTCGCGCAAGGATGAGT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7492R-1.in silico     61  AGACCGAGTTTCTCAGCAAGGAGAATTTACTTAGTGAACTGCAGTCGCGCAAGGATGAGT 120


7492R-1.IR_full       121 GCTATCACTTGAATTACTTTCTAGCCATAACAGAGAGCCAGTTTCGGTATCTAGTGCAAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7492R-1.in silico     121 GCTATCACTTGAATTACTTTCTAGCCATAACAGAGAGCCAGTTTCGGTATCTAGTGCAAA 180


7492R-1.IR_full       181 AGCTGGAGCCCATCATTAGCCA<span class="snp"><tt>N</tt></span>GTATGCT<span class="snp"><tt>G</tt></span>CCGCAACGCAAGAAGAAGTCCTTCAGCGC 240
                          ||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||
7492R-1.in silico     181 AGCTGGAGCCCATCATTAGCCA<span class="snp"><tt>-</tt></span>GTATGCT<span class="snp"><tt>-</tt></span>CCGCAACGCAAGAAGAAGTCCTTCAGCGC 240


7492R-1.IR_full       241 C<span class="snp"><tt>T</tt></span>GAGGAACGACTGGCCATAACGCTCAAGTATTTGGCAACGGGTGAAGTACATTCTTGTC 300
                          |<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7492R-1.in silico     241 C<span class="snp"><tt>-</tt></span>GAGGAACGACTGGCCATAACGCTCAAGTATTTGGCAACGGGTGAAGTACATTCTTGTC 300


7492R-1.IR_full       301 GAAACTACTGCTTCCGTGCCTCGAAATTTGTGATAAACGAAATGATTGCCAATATCTGTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7492R-1.in silico     301 GAAACTACTGCTTCCGTGCCTCGAAATTTGTGATAAACGAAATGATTGCCAATATCTGTC 360


7492R-1.IR_full       361 TGGGCTTCTACGAGCACCTCAAGGACCAATACGTGACACTACCAAAGACTGACGATCAG<span class="snp"><tt>N</tt></span> 420
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
7492R-1.in silico     361 TGGGCTTCTACGAGCACCTCAAGGACCAATACGTGACACTACCAAAGACTGACGATCAG<span class="snp"><tt>-</tt></span> 420


7492R-1.IR_full       421 TGGCGCAG<span class="snp"><tt>N</tt></span>GCCGCCG<span class="snp"><tt>A</tt></span>AGGAGATGGAGCGTAAGCACAACCTGCCCCACTGTGTGGGCAA 480
                          ||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||
7492R-1.in silico     421 TGGCGCAG<span class="snp"><tt>C</tt></span>GCCGCCG<span class="snp"><tt>-</tt></span>AGGAGATGGAGCGTAAGCACAACCTGCCCCACTGTGTGGGCAA 480


7492R-1.IR_full       481 TCTGTTCATG<span class="snp"><tt>N</tt></span>GCAGCATCCAACTC 505
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||
7492R-1.in silico     481 TCTGTTCATG<span class="snp"><tt>C</tt></span>GCAGCATCCAACTC 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139889.1	CG7492-RA (CG7492), mRNA
0	0	3	4	4	NM_133003.2	CG32555-RB, transcript variant B (RhoGAPp190), mRNA
0	0	3	4	4	NM_167573.1	CG32555-RC, transcript variant C (RhoGAPp190), mRNA
0	0	3	4	4	NM_167572.1	CG32555-RA, transcript variant A (RhoGAPp190), mRNA
0	0	2	3	3	NM_165439.1	CG17510-RB, transcript variant B (CG17510), mRNA
0	0	2	2	3	NM_167342.1	CG4407-RB, transcript variant B (CG4407), mRNA
0	0	2	2	3	NM_132609.2	CG4407-RA, transcript variant A (CG4407), mRNA
0	0	1	5	3	NM_137128.2	CG12864-RA, transcript variant A (Su(var)2-HP2), mRNA
0	0	1	4	4	NM_163879.1	CG32491-RD, transcript variant D (mod(mdg4)), mRNA
0	0	1	4	4	NM_080197.2	CG32491-RF, transcript variant F (mod(mdg4)), mRNA
0	0	1	4	4	NM_163878.1	CG32491-RA, transcript variant A (mod(mdg4)), mRNA
0	0	1	4	4	NM_163877.1	CG32491-RR, transcript variant R (mod(mdg4)), mRNA
0	0	1	4	4	NM_163881.1	CG32491-RQ, transcript variant Q (mod(mdg4)), mRNA
0	0	1	4	4	NM_163882.1	CG32491-RH, transcript variant H (mod(mdg4)), mRNA
0	0	1	4	4	NM_163880.1	CG32491-RG, transcript variant G (mod(mdg4)), mRNA
0	0	1	4	4	NM_163894.1	CG32491-RN, transcript variant N (mod(mdg4)), mRNA
0	0	1	4	4	NM_163893.1	CG32491-RO, transcript variant O (mod(mdg4)), mRNA
0	0	1	4	4	NM_163892.1	CG32491-RS, transcript variant S (mod(mdg4)), mRNA
0	0	1	4	4	NM_176527.1	CG32491-RU, transcript variant U (mod(mdg4)), mRNA
0	0	1	4	4	NM_176526.1	CG32491-RV, transcript variant V (mod(mdg4)), mRNA
0	0	1	4	4	NM_176525.1	CG32491-RW, transcript variant W (mod(mdg4)), mRNA
0	0	1	4	4	NM_176524.1	CG32491-RX, transcript variant X (mod(mdg4)), mRNA
0	0	1	4	4	NM_176523.1	CG32491-RY, transcript variant Y (mod(mdg4)), mRNA
0	0	1	4	4	NM_163891.1	CG32491-RC, transcript variant C (mod(mdg4)), mRNA
0	0	1	4	4	NM_163890.1	CG32491-RE, transcript variant E (mod(mdg4)), mRNA
0	0	1	4	4	NM_163889.1	CG32491-RM, transcript variant M (mod(mdg4)), mRNA
0	0	1	4	4	NM_176522.1	CG32491-RZ, transcript variant Z (mod(mdg4)), mRNA
0	0	1	4	4	NM_176521.1	CG32491-RT, transcript variant T (mod(mdg4)), mRNA
0	0	1	4	4	NM_163887.1	CG32491-RL, transcript variant L (mod(mdg4)), mRNA
0	0	1	4	4	NM_163888.1	CG32491-RK, transcript variant K (mod(mdg4)), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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