NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7479R-3

Symbol

Aats-leu

Full Name

Leucyl-tRNA synthetase

CG No

CG7479

Old CG No

CG7479

Synonyms

LeuRS, LRS, CG7479, Aats-leu

Accession No (Link to NCBI)

NM_139675.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Arsham AM, Neufeld TP.<br> A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway.<br> PLoS One (2009) 4(6) e6068 [ PubMed ID = 19562034 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTGGGGAGCTGTCTACTGGAA

Primer Seq. 3'

aatctagaggtaccCATGGCGATGGAGCATTAGGA

Predicted Fragment Size

IR fragment full Seq

0001 TTGGGGAGCT GTCTACTGGA ATAGCTGGCG GCGCCTGCTA ACCCAAAGCT GCACGGGGAA 
0061 TGATAATCCA GAGCTAACCA GTGATGTCAA GCACCGGATT GAGGCTCATT GGCGGGAACA 
0121 GCTCAGTGGA GGACAGTTCA ATCCCAAGGA CTCGCAGGAC AAGTACTACG TGCTCTCCAT 
0181 GTTCCCGTAT CCCTCCGGCA ATCTTCACAT GGGCCATGTG CGCCGTCTAT ACCATAGCCG 
0241 ATTCGGTGGC TCGTTTCCAG CGAATGTGCG GCAAGAATGT GTTTCAGCCA ATGGGCTGGG 
0301 ATTCCTTTGG GCTGCCCGCC GAGAACGCCG CCAATCAGCG TGGGGTGGAG CCCGCGTCCT 
0361 GGACGGAGCA GAACATTGCC CAGATGAAGG AACAACTCAA GCGACTTGGT TGCTCCTTTG 
0421 ACTGGAACCA TGAATTGTCC ACCTGCAGTC CAAAATACTA CAAGTGGACG CAGCATCTGT 
0481 TCCTAATGCT CCATCGCCAT G

in silico PCR Fragment

0001 TTGGGGAGCT GTCTACTGGA ATAGCTGGCG GCGCCTGCTA ACCCAAAGCT GCACCCGGAA 
0061 TGATAATCCA GAGCTAACCA GTGATGTCAA GCACCGGATT GAGGCTCATT GGCGGGAGCA 
0121 GCTCAGTGGA GGACAGTTCA ATCCCAAGGA CTCGCAGGAC AAGTACTACG TGCTCTCCAT 
0181 GTTCCCGTAT CCCTCCGGCA ATCTTCACAT GGGCCATGTG CG-CGTCTAT ACCATAGCCG 
0241 ATTCGGTGGC TCGTTTCCAG CGAATGTGCG GCAAGAATGT GTTTCAGCCA ATGGGTTGGG 
0301 ATTCCTTTGG GCTGCCCGCC GAGAACGCCG CCAATCAGCG TGGGGTGGAG CCCGCGTCCT 
0361 GGACGGAGCA GAACATTGCC CAGATGAAGG AACAACTCAA GCGACTTGGT TGCTCCTTTG 
0421 ACTGGAACCA TGAATTGTCC ACCTGCAGTC CAAAATACTA CAAGTGGACG CAGCATCTGT 
0481 TCCTAATGCT CCATCGCCAT G

Assemble Data

<PRE>

7479R-3.IR_full       1   TTGGGGAGCTGTCTACTGGAATAGCTGGCGGCGCCTGCTAACCCAAAGCTGCAC<span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span>GGAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||
7479R-3.in silico     1   TTGGGGAGCTGTCTACTGGAATAGCTGGCGGCGCCTGCTAACCCAAAGCTGCAC<span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span>GGAA 60


7479R-3.IR_full       61  TGATAATCCAGAGCTAACCAGTGATGTCAAGCACCGGATTGAGGCTCATTGGCGGGA<span class="snp"><tt>A</tt></span>CA 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||
7479R-3.in silico     61  TGATAATCCAGAGCTAACCAGTGATGTCAAGCACCGGATTGAGGCTCATTGGCGGGA<span class="snp"><tt>G</tt></span>CA 120


7479R-3.IR_full       121 GCTCAGTGGAGGACAGTTCAATCCCAAGGACTCGCAGGACAAGTACTACGTGCTCTCCAT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7479R-3.in silico     121 GCTCAGTGGAGGACAGTTCAATCCCAAGGACTCGCAGGACAAGTACTACGTGCTCTCCAT 180


7479R-3.IR_full       181 GTTCCCGTATCCCTCCGGCAATCTTCACATGGGCCATGTGCG<span class="snp"><tt>C</tt></span>CGTCTATACCATAGCCG 240
                          ||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||
7479R-3.in silico     181 GTTCCCGTATCCCTCCGGCAATCTTCACATGGGCCATGTGCG<span class="snp"><tt>-</tt></span>CGTCTATACCATAGCCG 240


7479R-3.IR_full       241 ATTCGGTGGCTCGTTTCCAGCGAATGTGCGGCAAGAATGTGTTTCAGCCAATGGG<span class="snp"><tt>C</tt></span>TGGG 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||
7479R-3.in silico     241 ATTCGGTGGCTCGTTTCCAGCGAATGTGCGGCAAGAATGTGTTTCAGCCAATGGG<span class="snp"><tt>T</tt></span>TGGG 300


7479R-3.IR_full       301 ATTCCTTTGGGCTGCCCGCCGAGAACGCCGCCAATCAGCGTGGGGTGGAGCCCGCGTCCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7479R-3.in silico     301 ATTCCTTTGGGCTGCCCGCCGAGAACGCCGCCAATCAGCGTGGGGTGGAGCCCGCGTCCT 360


7479R-3.IR_full       361 GGACGGAGCAGAACATTGCCCAGATGAAGGAACAACTCAAGCGACTTGGTTGCTCCTTTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7479R-3.in silico     361 GGACGGAGCAGAACATTGCCCAGATGAAGGAACAACTCAAGCGACTTGGTTGCTCCTTTG 420


7479R-3.IR_full       421 ACTGGAACCATGAATTGTCCACCTGCAGTCCAAAATACTACAAGTGGACGCAGCATCTGT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7479R-3.in silico     421 ACTGGAACCATGAATTGTCCACCTGCAGTCCAAAATACTACAAGTGGACGCAGCATCTGT 480


7479R-3.IR_full       481 TCCTAATGCTCCATCGCCATG 501
                          |||||||||||||||||||||
7479R-3.in silico     481 TCCTAATGCTCCATCGCCATG 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_139675.2	CG7479-RA (Aats-leu), mRNA
0	0	2	5	11	NM_134516.2	CG32529-RA, transcript variant A (CG32529), mRNA
0	0	2	5	7	NM_167688.1	CG32529-RC, transcript variant C (CG32529), mRNA
0	0	2	5	7	NM_001038766.1	CG32529-RD, transcript variant D (CG32529), mRNA
0	0	2	2	3	NM_080095.2	CG9124-RA, transcript variant A (eIF-3p40), mRNA
0	0	2	2	3	NM_205909.1	CG9124-RB, transcript variant B (eIF-3p40), mRNA
0	0	1	4	4	NM_001043006.1	CG33554-RB, transcript variant B (Nipped-A), mRNA
0	0	1	4	4	NM_001043007.1	CG33554-RC, transcript variant C (Nipped-A), mRNA
0	0	1	4	4	NM_001014499.2	CG33554-RA, transcript variant A (Nipped-A), mRNA
0	0	1	4	2	NM_135581.1	CG7329-RA (CG7329), mRNA
0	0	1	3	6	NM_142142.1	CG7292-RA (Rrp6), mRNA
0	0	1	3	5	NM_135080.1	CG12512-RA (CG12512), mRNA
0	0	1	3	5	NM_132515.2	CG1841-RA, transcript variant A (Tango10), mRNA
0	0	1	3	5	NM_167302.1	CG1841-RB, transcript variant B (Tango10), mRNA
0	0	1	3	2	NM_169828.1	CG31229-RA (CG31229), mRNA
0	0	1	2	3	NM_132212.2	CG1575-RA (CG1575), mRNA
0	0	1	2	2	NM_164603.1	CG12787-RB, transcript variant B (hoe1), mRNA
0	0	1	2	2	NM_175968.1	CG12787-RF, transcript variant F (hoe1), mRNA
0	0	1	2	2	NM_135032.1	CG12787-RC, transcript variant C (hoe1), mRNA
0	0	1	2	2	NM_175967.1	CG12787-RE, transcript variant E (hoe1), mRNA
0	0	1	2	2	NM_175966.1	CG12787-RD, transcript variant D (hoe1), mRNA
0	0	1	2	2	NM_057657.4	CG17158-RA (cpb), mRNA
0	0	1	2	2	NM_135031.3	CG12787-RA, transcript variant A (hoe1), mRNA
0	0	0	7	3	NM_167311.1	CG1847-RB, transcript variant B (CG1847), mRNA
0	0	0	7	3	NM_132530.2	CG1847-RA, transcript variant A (CG1847), mRNA
0	0	0	6	4	NM_140080.1	CG3335-RA (CG3335), mRNA
0	0	0	5	5	NM_142942.3	CG31140-RA, transcript variant A (CG31140), mRNA
0	0	0	5	4	NM_206552.1	CG31140-RC, transcript variant C (CG31140), mRNA
0	0	0	5	4	NM_206553.1	CG31140-RB, transcript variant B (CG31140), mRNA
0	0	0	5	3	NM_134670.2	CG3625-RB, transcript variant B (CG3625), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00970 : Aminoacyl-tRNA biosynthesis

Back