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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7427R-3

Symbol

CG7427

Full Name

CG7427

CG No

CG7427

Old CG No

CG7427

Synonyms

CG7427

Accession No (Link to NCBI)

NM_140520.1

Inserted Chr.

lll

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAATCCAGCACGCACCGCGATA

Primer Seq. 3'

aatctagaggtaccCAGGTCAATGCCCTTTTGCCC

Predicted Fragment Size

IR fragment full Seq

0001 AATCCAgCAC GCACCGcGAT AAGGTGAAGA AGTTCATATC GCTGACACAC ACGGGGGAAC 
0061 AGACGGCAAT ATTCTGTTTG CAACAGAACG ACTGGAAATT CGAGTTGGCC AGCGACAATT 
0121 ACTTCCAGAA TCCGGAGTAC TATTACCGGG AGTTGGATCG CAAGCGCNAT CGAGCAGCTA 
0181 TTCATGCGCT ATCGGGATCC CAGTGATCCC CTAAAGATCG GATCCCAGGG CGTAATCCAC 
0241 TTCCTGGAGG ACTTGGACCT GAAGCCAGAC TCAAAACTGG TGCTGATCAT AGCGTGGAAA 
0301 TTTCACGCCG AGGTACAGTG CGAGTTCTCG CGTGATGAGT TTATCAATGG CATGTGCGAC 
0361 CTGGGCATCG ATAGCATCGA CAAGCTAAAG ACCAAGCTGC CCATCCTTGA GCAGGAGCTG 
0421 AACGACGCCG GCAAGTTCAA GGACTTTTAC CACTTTACCT TCAACTACGC CAAGGATCCG 
0481 GGGCAAAAGG GCATTGACCT G

in silico PCR Fragment

0001 AATCCAGCAC GCACCGCGAT AAGGTGAAGA AGTTCATATC GCTGACACAC ACGGGGGAAC 
0061 AGACGGCAAT ATTCTGTTTG CAACAGAACG ACTGGAAATT CGAGTTGGCC AGCGACAATT 
0121 ACTTCCAGAA TCCGGAGTAC TATTACCGGG AGTTGGATCG CAAGCGC-AT CGAGCAGCTA 
0181 TTCATGCGCT ATCGGGATCC CAGTGATCCC CTAAAGATCG GATCCCAGGG CGTAATCCAC 
0241 TTCCTGGAGG ACTTGGACCT GAAGCCAGAC TCAAAACTGG TGCTGATCAT AGCGTGGAAA 
0301 TTCCACGCCG AGGTGCAGTG CGAGTTCTCG CGTGATGAGT TTATCAATGG CATGTGCGAC 
0361 CTGGGCATCG ATAGCATCGA CAAGCTAAAG ACCAAGCTGC CCATCCTTGA GCAGGAGCTG 
0421 AACGACGCCG GCAAGTTCAA GGACTTTTAC CACTTTACCT TCAACTACGC CAAGGATCCG 
0481 GGGCAAAAGG GCATTGACCT G

Assemble Data

<PRE>

7427R-3.IR_full       1   AATCCAGCACGCACCGCGATAAGGTGAAGAAGTTCATATCGCTGACACACACGGGGGAAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-3.in silico     1   AATCCAGCACGCACCGCGATAAGGTGAAGAAGTTCATATCGCTGACACACACGGGGGAAC 60


7427R-3.IR_full       61  AGACGGCAATATTCTGTTTGCAACAGAACGACTGGAAATTCGAGTTGGCCAGCGACAATT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-3.in silico     61  AGACGGCAATATTCTGTTTGCAACAGAACGACTGGAAATTCGAGTTGGCCAGCGACAATT 120


7427R-3.IR_full       121 ACTTCCAGAATCCGGAGTACTATTACCGGGAGTTGGATCGCAAGCGC<span class="snp"><tt>N</tt></span>ATCGAGCAGCTA 180
                          |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
7427R-3.in silico     121 ACTTCCAGAATCCGGAGTACTATTACCGGGAGTTGGATCGCAAGCGC<span class="snp"><tt>-</tt></span>ATCGAGCAGCTA 180


7427R-3.IR_full       181 TTCATGCGCTATCGGGATCCCAGTGATCCCCTAAAGATCGGATCCCAGGGCGTAATCCAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-3.in silico     181 TTCATGCGCTATCGGGATCCCAGTGATCCCCTAAAGATCGGATCCCAGGGCGTAATCCAC 240


7427R-3.IR_full       241 TTCCTGGAGGACTTGGACCTGAAGCCAGACTCAAAACTGGTGCTGATCATAGCGTGGAAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-3.in silico     241 TTCCTGGAGGACTTGGACCTGAAGCCAGACTCAAAACTGGTGCTGATCATAGCGTGGAAA 300


7427R-3.IR_full       301 TT<span class="snp"><tt>T</tt></span>CACGCCGAGGT<span class="snp"><tt>A</tt></span>CAGTGCGAGTTCTCGCGTGATGAGTTTATCAATGGCATGTGCGAC 360
                          ||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||
7427R-3.in silico     301 TT<span class="snp"><tt>C</tt></span>CACGCCGAGGT<span class="snp"><tt>G</tt></span>CAGTGCGAGTTCTCGCGTGATGAGTTTATCAATGGCATGTGCGAC 360


7427R-3.IR_full       361 CTGGGCATCGATAGCATCGACAAGCTAAAGACCAAGCTGCCCATCCTTGAGCAGGAGCTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-3.in silico     361 CTGGGCATCGATAGCATCGACAAGCTAAAGACCAAGCTGCCCATCCTTGAGCAGGAGCTG 420


7427R-3.IR_full       421 AACGACGCCGGCAAGTTCAAGGACTTTTACCACTTTACCTTCAACTACGCCAAGGATCCG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-3.in silico     421 AACGACGCCGGCAAGTTCAAGGACTTTTACCACTTTACCTTCAACTACGCCAAGGATCCG 480


7427R-3.IR_full       481 GGGCAAAAGGGCATTGACCTG 501
                          |||||||||||||||||||||
7427R-3.in silico     481 GGGCAAAAGGGCATTGACCTG 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_140520.1	CG7427-RA (CG7427), mRNA
0.41	2	3	5	4	NM_142273.2	CG12785-RA (CG12785), mRNA
0	0	4	7	2	NM_001038780.1	CG33979-RA, transcript variant A (capt), mRNA
0	0	4	7	2	NM_001038781.1	CG33979-RB, transcript variant B (capt), mRNA
0	0	3	3	4	NM_143291.1	CG5984-RA (CG5984), mRNA
0	0	3	2	9	NM_001014545.1	CG33519-RB (Unc-89), mRNA
0	0	2	3	2	NM_143791.2	CG6421-RA (CG6421), mRNA
0	0	2	2	6	NM_166126.1	CG18255-RC, transcript variant C (Strn-Mlck), mRNA
0	0	2	2	5	NM_166125.2	CG18255-RA, transcript variant A (Strn-Mlck), mRNA
0	0	2	2	3	NM_166127.1	CG18255-RF, transcript variant F (Strn-Mlck), mRNA
0	0	2	2	3	NM_079030.2	CG18255-RB, transcript variant B (Strn-Mlck), mRNA
0	0	2	2	3	NM_166128.1	CG18255-RG, transcript variant G (Strn-Mlck), mRNA
0	0	1	4	3	NM_140904.1	CG8004-RA (CG8004), mRNA
0	0	1	4	3	NM_143315.1	CG5612-RA (CG5612), mRNA
0	0	1	4	2	NM_057543.3	CG9998-RA (U2af50), mRNA
0	0	1	3	8	NM_139676.1	CG13708-RA (CG13708), mRNA
0	0	1	2	12	NM_078664.2	CG5870-RA (beta-Spec), mRNA
0	0	1	2	6	NM_166623.1	CG4051-RA (egl), mRNA
0	0	1	2	6	NM_079144.3	CG6821-RA (Lsp1gamma), mRNA
0	0	1	2	4	NM_140267.1	CG14126-RA (CG14126), mRNA
0	0	1	2	4	NM_141899.2	CG4702-RA (CG4702), mRNA
0	0	1	2	4	NM_130606.2	CG3573-RA (CG3573), mRNA
0	0	1	2	3	NM_140769.1	CG5290-RA (CG5290), mRNA
0	0	0	6	7	NM_134490.2	CG12238-RA (l(1)G0084), mRNA
0	0	0	6	2	NM_057954.2	CG5005-RA (HLH54F), mRNA
0	0	0	5	5	NM_136934.2	CG13157-RA (CG13157), mRNA
0	0	0	5	4	NM_078921.2	CG4485-RA (Cyp9b1), mRNA
0	0	0	4	6	NM_167356.1	CG1770-RB, transcript variant B (HDAC4), mRNA
0	0	0	4	6	NM_001014620.1	CG9918-RD (CG9918), mRNA
0	0	0	4	6	NM_132509.1	CG15220-RA (CG15220), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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