|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7427R-2 |
| Symbol |
CG7427 |
Full Name |
CG7427 |
| CG No |
CG7427 |
Old CG No |
CG7427 |
| Synonyms |
CG7427 |
| Accession No (Link to NCBI) |
NM_140520.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.<br> Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.<br> PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAATCCAGCACGCACCGCGATA |
| Primer Seq. 3' |
aatctagaggtaccCAGGTCAATGCCCTTTTGCCC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AATCCAgCAC GCACCGcGAT AAGGTGAAGA AGTTCATATC GCTGACACAC ACGGGGGAAC
0061 AGACGGCAAT ATTCTGTTTG CAACAGAACG ACTGGAAATT CGAGTTGGCC AGCGACAATT
0121 ACTTCCAGAA TCCGGAGTAC TATTACCGGG AGTTGGATCG CAAGCGCNAT CGAGCAGCTA
0181 TTCATGCGCT ATCGGGATCC CAGTGATCCC CTAAAGATCG GATCCCAGGG CGTAATCCAC
0241 TTCCTGGAGG ACTTGGACCT GAAGCCAGAC TCAAAACTGG TGCTGATCAT AGCGTGGAAA
0301 TTTCACGCCG AGGTACAGTG CGAGTTCTCG CGTGATGAGT TTATCAATGG CATGTGCGAC
0361 CTGGGCATCG ATAGCATCGA CAAGCTAAAG ACCAAGCTGC CCATCCTTGA GCAGGAGCTG
0421 AACGACGCCG GCAAGTTCAA GGACTTTTAC CACTTTACCT TCAACTACGC CAAGGATCCG
0481 GGGCAAAAGG GCATTGACCT G |
| in silico PCR Fragment |
0001 AATCCAGCAC GCACCGCGAT AAGGTGAAGA AGTTCATATC GCTGACACAC ACGGGGGAAC
0061 AGACGGCAAT ATTCTGTTTG CAACAGAACG ACTGGAAATT CGAGTTGGCC AGCGACAATT
0121 ACTTCCAGAA TCCGGAGTAC TATTACCGGG AGTTGGATCG CAAGCGC-AT CGAGCAGCTA
0181 TTCATGCGCT ATCGGGATCC CAGTGATCCC CTAAAGATCG GATCCCAGGG CGTAATCCAC
0241 TTCCTGGAGG ACTTGGACCT GAAGCCAGAC TCAAAACTGG TGCTGATCAT AGCGTGGAAA
0301 TTCCACGCCG AGGTGCAGTG CGAGTTCTCG CGTGATGAGT TTATCAATGG CATGTGCGAC
0361 CTGGGCATCG ATAGCATCGA CAAGCTAAAG ACCAAGCTGC CCATCCTTGA GCAGGAGCTG
0421 AACGACGCCG GCAAGTTCAA GGACTTTTAC CACTTTACCT TCAACTACGC CAAGGATCCG
0481 GGGCAAAAGG GCATTGACCT G |
| Assemble Data |
<PRE>
7427R-2.IR_full 1 AATCCAGCACGCACCGCGATAAGGTGAAGAAGTTCATATCGCTGACACACACGGGGGAAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-2.in silico 1 AATCCAGCACGCACCGCGATAAGGTGAAGAAGTTCATATCGCTGACACACACGGGGGAAC 60
7427R-2.IR_full 61 AGACGGCAATATTCTGTTTGCAACAGAACGACTGGAAATTCGAGTTGGCCAGCGACAATT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-2.in silico 61 AGACGGCAATATTCTGTTTGCAACAGAACGACTGGAAATTCGAGTTGGCCAGCGACAATT 120
7427R-2.IR_full 121 ACTTCCAGAATCCGGAGTACTATTACCGGGAGTTGGATCGCAAGCGC<span class="snp"><tt>N</tt></span>ATCGAGCAGCTA 180
|||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||
7427R-2.in silico 121 ACTTCCAGAATCCGGAGTACTATTACCGGGAGTTGGATCGCAAGCGC<span class="snp"><tt>-</tt></span>ATCGAGCAGCTA 180
7427R-2.IR_full 181 TTCATGCGCTATCGGGATCCCAGTGATCCCCTAAAGATCGGATCCCAGGGCGTAATCCAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-2.in silico 181 TTCATGCGCTATCGGGATCCCAGTGATCCCCTAAAGATCGGATCCCAGGGCGTAATCCAC 240
7427R-2.IR_full 241 TTCCTGGAGGACTTGGACCTGAAGCCAGACTCAAAACTGGTGCTGATCATAGCGTGGAAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-2.in silico 241 TTCCTGGAGGACTTGGACCTGAAGCCAGACTCAAAACTGGTGCTGATCATAGCGTGGAAA 300
7427R-2.IR_full 301 TT<span class="snp"><tt>T</tt></span>CACGCCGAGGT<span class="snp"><tt>A</tt></span>CAGTGCGAGTTCTCGCGTGATGAGTTTATCAATGGCATGTGCGAC 360
||<span class="snp"><tt> </tt></span>|||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||
7427R-2.in silico 301 TT<span class="snp"><tt>C</tt></span>CACGCCGAGGT<span class="snp"><tt>G</tt></span>CAGTGCGAGTTCTCGCGTGATGAGTTTATCAATGGCATGTGCGAC 360
7427R-2.IR_full 361 CTGGGCATCGATAGCATCGACAAGCTAAAGACCAAGCTGCCCATCCTTGAGCAGGAGCTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-2.in silico 361 CTGGGCATCGATAGCATCGACAAGCTAAAGACCAAGCTGCCCATCCTTGAGCAGGAGCTG 420
7427R-2.IR_full 421 AACGACGCCGGCAAGTTCAAGGACTTTTACCACTTTACCTTCAACTACGCCAAGGATCCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7427R-2.in silico 421 AACGACGCCGGCAAGTTCAAGGACTTTTACCACTTTACCTTCAACTACGCCAAGGATCCG 480
7427R-2.IR_full 481 GGGCAAAAGGGCATTGACCTG 501
|||||||||||||||||||||
7427R-2.in silico 481 GGGCAAAAGGGCATTGACCTG 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
3 |
NM_140520.1 |
CG7427-RA (CG7427), mRNA |
| 0.41 |
2 |
3 |
5 |
4 |
NM_142273.2 |
CG12785-RA (CG12785), mRNA |
| 0 |
0 |
4 |
7 |
2 |
NM_001038780.1 |
CG33979-RA, transcript variant A (capt), mRNA |
| 0 |
0 |
4 |
7 |
2 |
NM_001038781.1 |
CG33979-RB, transcript variant B (capt), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_143291.1 |
CG5984-RA (CG5984), mRNA |
| 0 |
0 |
3 |
2 |
9 |
NM_001014545.1 |
CG33519-RB (Unc-89), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_143791.2 |
CG6421-RA (CG6421), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_166126.1 |
CG18255-RC, transcript variant C (Strn-Mlck), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_166127.1 |
CG18255-RF, transcript variant F (Strn-Mlck), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079030.2 |
CG18255-RB, transcript variant B (Strn-Mlck), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_166128.1 |
CG18255-RG, transcript variant G (Strn-Mlck), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_140904.1 |
CG8004-RA (CG8004), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_143315.1 |
CG5612-RA (CG5612), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_057543.3 |
CG9998-RA (U2af50), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_139676.1 |
CG13708-RA (CG13708), mRNA |
| 0 |
0 |
1 |
2 |
12 |
NM_078664.2 |
CG5870-RA (beta-Spec), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_166623.1 |
CG4051-RA (egl), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_079144.3 |
CG6821-RA (Lsp1gamma), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140267.1 |
CG14126-RA (CG14126), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141899.2 |
CG4702-RA (CG4702), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_130606.2 |
CG3573-RA (CG3573), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140769.1 |
CG5290-RA (CG5290), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_134490.2 |
CG12238-RA (l(1)G0084), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_057954.2 |
CG5005-RA (HLH54F), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_136934.2 |
CG13157-RA (CG13157), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_078921.2 |
CG4485-RA (Cyp9b1), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_167356.1 |
CG1770-RB, transcript variant B (HDAC4), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_001014620.1 |
CG9918-RD (CG9918), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_132509.1 |
CG15220-RA (CG15220), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| +- |
|
| DC |
 |
| +- |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| + |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
