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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7323R-2 
 Symbol CG7323  Full Name CG7323 
 CG No CG7323  Old CG No CG7323 
 Synonyms CG7323 
 Accession No (Link to NCBI) NM_140925.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCTCCACCACGTCTACTCATGC 
 Primer Seq. 3' 
aatctagaggtaccGGCTCTCCACATCGGTCTGCT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CTCCACCACG TCTACTCATG CTGTACCCTC CTCCAAACAC TTTCAGTCTG TTGGCGAGGC 
0061 GGCGGCCACA TCATCGTCCT CGACGCTGAG TCCGCACAGT GCTACATCCG CGACCTTAGG 
0121 CGGCGGCGGA GGTGGTGGCG GTGCAGGCGG TGGTGGTCTA ACCTCCCCGG TTGGCTGCTC 
0181 CACGTCCTCC GGTTCGACGG TGAAAAAGAA ATCCTCGTTC ATGAAGCGCA AGAAGCCANC 
0241 TGCTGACGCG CAGCGAGGTA TCCAGTTCCG AGTTCTTTTC CGTGTCATTC TGCTTGGACT 
0301 CGTCGCAGCA ACCAGATGAG GAGTTCATTC CGGCAACCAA AGGCGTGACA CTACTTAATG 
0361 CACTCAGCCA CGCCTTGCGT AGGCGGAACC TCAGCTTTAC ACAAATCACC ATTACGGACA 
0421 ATAATCCCAC GCCCAGTTTT TTAGATGCAG GTCCTTCGCT CCTGCCCGTT TCCGTGGACG 
0481 AGCAGACCGA TGTGGAGAGN C 
 in silico PCR Fragment 
0001 CTCCACCACG TCTACTCATG CTGTACCCTC CTCCAAACAC TTTCAGTCTG TTGGCGAGGC 
0061 GGCGGCCACA TCATCGTCCT CGACGCTGAG TCCGCACAGT GCTACATCCG CGACCTTAGG 
0121 CGGCGGCGGA GGTGGTGGCG GTGCAGGCGG TGGTGGTCTA ACCTCCCCGG TTGGCTGCTC 
0181 CACGTCCTCC GGTTCGACGG TGAAAAAGAA ATCCTCGTTC ATGAAGCGCA AGAAGCCA-C 
0241 TGCTGACGCG CAGCGAGGTA TCCAGTTCCG AGTTCTTTTC CGTGTCATTC TGCTTGGACT 
0301 CGTCGCAGCA ACCAGATGAG GAGTTCATTC CGGCAACCAA AGGCGTGACA CTACTTAATG 
0361 CACTCAGCCA CGCCTTGCGT AGGCGGAACC TCAGCTTTAC ACAAATCACC ATTACGGACA 
0421 ATAATCCCAC GCCCAGTTTT TTAGATGCAG GTCCTTCGCT CCTGCCCGTT TCCGTGGACG 
0481 AGCAGACCGA TGTGGAGAGC C 
 Assemble Data 
<PRE>

7323R-2.IR_full       1   CTCCACCACGTCTACTCATGCTGTACCCTCCTCCAAACACTTTCAGTCTGTTGGCGAGGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7323R-2.in silico     1   CTCCACCACGTCTACTCATGCTGTACCCTCCTCCAAACACTTTCAGTCTGTTGGCGAGGC 60


7323R-2.IR_full       61  GGCGGCCACATCATCGTCCTCGACGCTGAGTCCGCACAGTGCTACATCCGCGACCTTAGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7323R-2.in silico     61  GGCGGCCACATCATCGTCCTCGACGCTGAGTCCGCACAGTGCTACATCCGCGACCTTAGG 120


7323R-2.IR_full       121 CGGCGGCGGAGGTGGTGGCGGTGCAGGCGGTGGTGGTCTAACCTCCCCGGTTGGCTGCTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7323R-2.in silico     121 CGGCGGCGGAGGTGGTGGCGGTGCAGGCGGTGGTGGTCTAACCTCCCCGGTTGGCTGCTC 180


7323R-2.IR_full       181 CACGTCCTCCGGTTCGACGGTGAAAAAGAAATCCTCGTTCATGAAGCGCAAGAAGCCA<span class="snp"><tt>N</tt></span>C 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
7323R-2.in silico     181 CACGTCCTCCGGTTCGACGGTGAAAAAGAAATCCTCGTTCATGAAGCGCAAGAAGCCA<span class="snp"><tt>-</tt></span>C 240


7323R-2.IR_full       241 TGCTGACGCGCAGCGAGGTATCCAGTTCCGAGTTCTTTTCCGTGTCATTCTGCTTGGACT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7323R-2.in silico     241 TGCTGACGCGCAGCGAGGTATCCAGTTCCGAGTTCTTTTCCGTGTCATTCTGCTTGGACT 300


7323R-2.IR_full       301 CGTCGCAGCAACCAGATGAGGAGTTCATTCCGGCAACCAAAGGCGTGACACTACTTAATG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7323R-2.in silico     301 CGTCGCAGCAACCAGATGAGGAGTTCATTCCGGCAACCAAAGGCGTGACACTACTTAATG 360


7323R-2.IR_full       361 CACTCAGCCACGCCTTGCGTAGGCGGAACCTCAGCTTTACACAAATCACCATTACGGACA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7323R-2.in silico     361 CACTCAGCCACGCCTTGCGTAGGCGGAACCTCAGCTTTACACAAATCACCATTACGGACA 420


7323R-2.IR_full       421 ATAATCCCACGCCCAGTTTTTTAGATGCAGGTCCTTCGCTCCTGCCCGTTTCCGTGGACG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7323R-2.in silico     421 ATAATCCCACGCCCAGTTTTTTAGATGCAGGTCCTTCGCTCCTGCCCGTTTCCGTGGACG 480


7323R-2.IR_full       481 AGCAGACCGATGTGGAGAG<span class="snp"><tt>N</tt></span>C 501
                          |||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
7323R-2.in silico     481 AGCAGACCGATGTGGAGAG<span class="snp"><tt>C</tt></span>C 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  1  26  NM_140925.2  CG7323-RA (CG7323), mRNA 
1.03   5  3  4  21  NM_132535.2  CG1844-RA (SelG), mRNA 
0.41   2  6  14  25  NM_139645.2  CG15021-RA (CG15021), mRNA 
0.41   2  5  9  18  NM_057829.3  CG8075-RA (Vang), mRNA 
0.41   2  5  2  19  NM_142397.1  CG14318-RA (CG14318), mRNA 
0.41   2  3  4  9  NM_132302.3  CG7055-RA (dalao), mRNA 
0   0  15  40  123  NM_167493.1  CG3606-RA, transcript variant A (caz), mRNA 
0   0  15  40  123  NM_078641.2  CG3606-RB, transcript variant B (caz), mRNA 
0   0  7  7  16  NM_133014.2  CG6269-RA (unc-4), mRNA 
0   0  7  2  18  NM_167279.1  CG18361-RB, transcript variant B (dsh), mRNA 
0   0  7  2  18  NM_078563.2  CG18361-RA, transcript variant A (dsh), mRNA 
0   0  6  20  49  NM_132976.1  CG5172-RA, transcript variant A (CG5172), mRNA 
0   0  6  11  23  NM_206140.1  CG8912-RC, transcript variant C (Psi), mRNA 
0   0  6  11  23  NM_057775.3  CG8912-RB, transcript variant B (Psi), mRNA 
0   0  6  11  23  NM_166200.2  CG8912-RA, transcript variant A (Psi), mRNA 
0   0  6  11  22  NM_206362.1  CG9425-RB, transcript variant B (CG9425), mRNA 
0   0  6  11  22  NM_140468.2  CG9425-RA, transcript variant A (CG9425), mRNA 
0   0  6  7  14  NM_169947.1  CG17299-RF, transcript variant F (SNF4Agamma), mRNA 
0   0  6  6  8  NM_080124.3  CG31695-RA (scw), mRNA 
0   0  6  5  21  NM_167505.1  CG4211-RB, transcript variant B (nonA), mRNA 
0   0  6  5  21  NM_001014747.1  CG4211-RC, transcript variant C (nonA), mRNA 
0   0  6  5  21  NM_078643.2  CG4211-RA, transcript variant A (nonA), mRNA 
0   0  6  5  5  NM_078659.3  CG5055-RA, transcript variant A (baz), mRNA 
0   0  6  5  4  NM_001042818.1  CG5055-RB, transcript variant B (baz), mRNA 
0   0  6  4  21  NM_132773.2  CG5627-RA (rab3-GEF), mRNA 
0   0  5  7  22  NM_176254.1  CG5403-RB, transcript variant B (retn), mRNA 
0   0  5  7  22  NM_057516.3  CG5403-RA, transcript variant A (retn), mRNA 
0   0  5  6  37  NM_132831.1  CG8184-RB (CG8184), mRNA 
0   0  4  20  58  NM_132536.1  CG1840-RA (CG1840), mRNA 
0   0  4  20  41  NM_137510.2  CG30122-RB (CG30122), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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