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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7306R-2 
 Symbol obst-F  Full Name obstructor-F 
 CG No CG7306  Old CG No CG7306 
 Synonyms obst-F, CG7306 
 Accession No (Link to NCBI) NM_140929.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal 
 Map Viewer
 Reference
[Please submit your publication]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgTGGGCATATGTCCTGGCATTG 
 Primer Seq. 3'
aatctagaggtaccTAGGCGCAGATGAAGTACAGT 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 TGGGCATATG TCCTGGCATT GAGCATCTGC TTCCAGTTGG GAGCCGGTCA CGCTGTGGAC 
0061 CAAAGTTGGG AACTGCCCAA AGTTCGACAT ACTGTGGGTG ACCTCAGCCA TATCTGCTTG 
0121 GGACGTCAAG AGGGCGATTT GGTGCCACAT CCGTTGGATT GTAATGGATA TTTCTCCTGC 
0181 TCGCGTGTGC CCACTCTACT CTACTGCGAC CAGGGCCTTC AGTTCGACGA GAACCGTGNN 
0241 ATATGCGATC TGCCGGAGAA TACCAACTGT CGTCCCGTAG CCACTGGCAC TGTGGAATCC 
0301 GCAAATGGCC TGGCGGATAA CTCCGAGCTC AACTGGTGGC CACACAAgNC GAAGCCCGTC 
0361 TTTGTGGCCG TCGATGTGAC CAGTGGTCAG CCGGTTAATC CCATGGAGAA GTACGATCCG 
0421 GAGCATATCG AGTGCCGTCA CTATGGAGCC TACTTCCTGC CGCACCCCAG AAATTGTGGA 
0481 CTGTACTTCA TCTGCGCCTA  
 in silico PCR Fragment
0001 TGGGCATATG TCCTGGCATT GAGCATCTGC TTCCAGTTGG GAGCCGGTCA CGCTGTGGAC 
0061 CAAAGTTGGG AACTGCCCAA AGTTCGACAT ACTGTGGGTC ACCTCAGCCA TATCTGCTTG 
0121 GGACGTCAAG AGGGCGATTT GGTGCCACAT CCGTTGGATT GTAATGGATA TTTCTCCTGC 
0181 TCGCGTGTGC CCACTCTACT CTACTGCGAC CAGGGCCTTC AGTTCGACGA GAACCGTGCG 
0241 ATATGCGATC TGCCGGAGAA TACCAACTGT CGTCCCGTAG CCACTGGCAC TGTGGAATCC 
0301 GCAAATGGCC TGGCGGATAA CTCCGAGCTC AACTGGTGGC CACACAAGCC GAAGCCCGTC 
0361 TTTGTGGCCG TCGATGTGAC CAGTGGTCAG CCGGTTAATC CCATGGAGAA GTACGATCCG 
0421 GAGCATATCG AGTGCCGTCA CTATGGAGCC TACTTCCTGC CGCACCCCAG AAATTGTGGA 
0481 CTGTACTTCA TCTGCGCCTA  
 Assemble Data

7306R-2.IR_full       1   TGGGCATATGTCCTGGCATTGAGCATCTGCTTCCAGTTGGGAGCCGGTCACGCTGTGGAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7306R-2.in silico     1   TGGGCATATGTCCTGGCATTGAGCATCTGCTTCCAGTTGGGAGCCGGTCACGCTGTGGAC 60


7306R-2.IR_full       61  CAAAGTTGGGAACTGCCCAAAGTTCGACATACTGTGGGTGACCTCAGCCATATCTGCTTG 120
                          ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
7306R-2.in silico     61  CAAAGTTGGGAACTGCCCAAAGTTCGACATACTGTGGGTCACCTCAGCCATATCTGCTTG 120


7306R-2.IR_full       121 GGACGTCAAGAGGGCGATTTGGTGCCACATCCGTTGGATTGTAATGGATATTTCTCCTGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7306R-2.in silico     121 GGACGTCAAGAGGGCGATTTGGTGCCACATCCGTTGGATTGTAATGGATATTTCTCCTGC 180


7306R-2.IR_full       181 TCGCGTGTGCCCACTCTACTCTACTGCGACCAGGGCCTTCAGTTCGACGAGAACCGTGNN 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||  
7306R-2.in silico     181 TCGCGTGTGCCCACTCTACTCTACTGCGACCAGGGCCTTCAGTTCGACGAGAACCGTGCG 240


7306R-2.IR_full       241 ATATGCGATCTGCCGGAGAATACCAACTGTCGTCCCGTAGCCACTGGCACTGTGGAATCC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7306R-2.in silico     241 ATATGCGATCTGCCGGAGAATACCAACTGTCGTCCCGTAGCCACTGGCACTGTGGAATCC 300


7306R-2.IR_full       301 GCAAATGGCCTGGCGGATAACTCCGAGCTCAACTGGTGGCCACACAAGNCGAAGCCCGTC 360
                          |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
7306R-2.in silico     301 GCAAATGGCCTGGCGGATAACTCCGAGCTCAACTGGTGGCCACACAAGCCGAAGCCCGTC 360


7306R-2.IR_full       361 TTTGTGGCCGTCGATGTGACCAGTGGTCAGCCGGTTAATCCCATGGAGAAGTACGATCCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7306R-2.in silico     361 TTTGTGGCCGTCGATGTGACCAGTGGTCAGCCGGTTAATCCCATGGAGAAGTACGATCCG 420


7306R-2.IR_full       421 GAGCATATCGAGTGCCGTCACTATGGAGCCTACTTCCTGCCGCACCCCAGAAATTGTGGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7306R-2.in silico     421 GAGCATATCGAGTGCCGTCACTATGGAGCCTACTTCCTGCCGCACCCCAGAAATTGTGGA 480


7306R-2.IR_full       481 CTGTACTTCATCTGCGCCTA 500
                          ||||||||||||||||||||
7306R-2.in silico     481 CTGTACTTCATCTGCGCCTA 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_140929.1  CG7306-RA (CG7306), mRNA 
0.2   NM_136362.2  CG8345-RA (Cyp6w1), mRNA 
0   NM_206497.1  CG10388-RF, transcript variant F (Ubx), mRNA 
0   NM_169730.1  CG10388-RC, transcript variant C (Ubx), mRNA 
0   NM_080504.2  CG10388-RA, transcript variant A (Ubx), mRNA 
0   NM_080500.2  CG10388-RB, transcript variant B (Ubx), mRNA 
0   NM_169728.1  CG10388-RD, transcript variant D (Ubx), mRNA 
0   NM_169729.1  CG10388-RE, transcript variant E (Ubx), mRNA 
0   12  NM_001014545.1  CG33519-RB (Unc-89), mRNA 
0   NM_136458.2  CG30494-RA (CG30494), mRNA 
0   NM_164577.1  CG15427-RC, transcript variant C (tutl), mRNA 
0   NM_164578.1  CG15427-RA, transcript variant A (tutl), mRNA 
0   NM_080127.4  CG15427-RE, transcript variant E (tutl), mRNA 
0   NM_164576.2  CG15427-RD, transcript variant D (tutl), mRNA 
0   NM_079198.2  CG15010-RC, transcript variant C (ago), mRNA 
0   NM_168073.1  CG15010-RB, transcript variant B (ago), mRNA 
0   NM_168072.1  CG15010-RA, transcript variant A (ago), mRNA 
0   NM_001038850.1  CG9027-RC, transcript variant C (CG9027), mRNA 
0   NM_134529.2  CG9575-RA (Rab35), mRNA 
0   NM_168756.1  CG8127-RC, transcript variant C (Eip75B), mRNA 
0   NM_078596.2  CG1372-RA, transcript variant A (yl), mRNA 
0   NM_206710.1  CG1372-RB, transcript variant B (yl), mRNA 
0   NM_132498.1  CG1572-RA, transcript variant A (CG1572), mRNA 
0   NM_167289.1  CG1572-RB, transcript variant B (CG1572), mRNA 
0   NM_166772.1  CG1862-RD, transcript variant D (Ephrin), mRNA 
0   NM_166771.1  CG1862-RC, transcript variant C (Ephrin), mRNA 
0   NM_143670.4  CG1862-RB, transcript variant B (Ephrin), mRNA 
0   NM_166770.1  CG1862-RA, transcript variant A (Ephrin), mRNA 
0   NM_079770.2  CG7748-RA (OstStt3), mRNA 
0   NM_168053.1  CG32254-RA (CG32254), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
DC
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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