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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7298R-3

Symbol

CG7298

Full Name

CG7298

CG No

CG7298

Old CG No

CG7298

Synonyms

anon-EST:ParkEST398, CG7298

Accession No (Link to NCBI)

NM_140930.3

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGACAGGCAAACTTCTACTGG

Primer Seq. 3'

aatctagaggtaccGAAATATTGGCCAGGTGGCAC

Predicted Fragment Size

IR fragment full Seq

0001 TGACAGGCAA ACTTCTACTG GCCACGATCC TGTGCCTAAT GGGCGGCCAA GCCtTGGGCN 
0061 NTGCGATTCT GGAAGGAAAC TACAACGTTA CGGCCGTCTG CACGNCGGNT AAGGTCGGAA 
0121 CGCAGCTGGG CAGCATCGAA TCCTGCCAGA CGTACTACGT CTGCCAGTCC ACCGGACCCG 
0181 TGCAGTCCTC GTGCCAATCA GGCTACTCGT ACGACTACAA GAGATCCTCC TGCTATCCAA 
0241 GCAGCGAGGT GGACTGCTAT TGGGGCGTTG AGAATCCCTG TGCTGGCAAG AATAACACCT 
0301 GGGTGCCAAN ACACTGCCGT TTGTGGTGGA TGGTTCTANC TGCCTTGAGG GCAAGTCGGC 
0361 GGGCTCCGGA AACTGCCCGG TGAACCAGAA ATTCGATACC ACCACACTCG CCTGCGTTTA 
0421 TGGAACCTGC TCCAACACTC AAGGTACTAA TGAAACCGTG CTAGAATCAC TATGCGATGT 
0481 CGTNCCACCT GGCCAATATT NN

in silico PCR Fragment

0001 TGACAGGCAA ACTTCTACTG GCCACGATCC TGTGCCTAAT GGGCGGCCAA GCCTTGGGCG 
0061 ATGCGATTCT GGAAGGAAAC TACAACGTTA CGGCCGTCTG CACGGCGGTT AAGGTCGGAA 
0121 CGCAGCTGGG CAGCATCGAA TCCTGCCAGA CGTACTACGT CTGCCAGTCC ACCGGACCCG 
0181 TGCAGTCCTC GTGCCAATCA GGCTACTCGT ACGACTACAA GAGATCCTCC TGCTATCCAA 
0241 GCAGCGAGGT GGACTGCTAT TGGGGCGTTG AGAATCCCTG TGCTGGCAAG AATAACACCT 
0301 GGGTGCCAA- ACACTGCCGT TTGTGGTGGA TGGTTCTA-C TGCCTTGAGG GCAAGTCGGC 
0361 GGGCTCCGGA AACTGCCCGG TGAACCAGAA ATTCGATACC ACCACACTCG CCTGCGTTTA 
0421 TGGAACCTGC TCCAACACTC AAGGTACTAA TGAAACCGTG CTAGAATCAC TATGCGATGT 
0481 CGTGCCACCT GGCCAATATT TC

Assemble Data

<PRE>

7298R-3.IR_full       1   TGACAGGCAAACTTCTACTGGCCACGATCCTGTGCCTAATGGGCGGCCAAGCCTTGGGC<span class="snp"><tt>N</tt></span> 60
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
7298R-3.in silico     1   TGACAGGCAAACTTCTACTGGCCACGATCCTGTGCCTAATGGGCGGCCAAGCCTTGGGC<span class="snp"><tt>G</tt></span> 60


7298R-3.IR_full        61  TGCGATTCTGGAAGGAAACTACAACGTTACGGCCGTCTGCACG<span class="snp"><tt>N</tt></span>CGG<span class="snp"><tt>N</tt></span>TAAGGTCGGAA 119
                           |||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||
7298R-3.in silico      61  TGCGATTCTGGAAGGAAACTACAACGTTACGGCCGTCTGCACG<span class="snp"><tt>G</tt></span>CGG<span class="snp"><tt>T</tt></span>TAAGGTCGGAA 119


7298R-3.IR_full       121 CGCAGCTGGGCAGCATCGAATCCTGCCAGACGTACTACGTCTGCCAGTCCACCGGACCCG 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7298R-3.in silico     121 CGCAGCTGGGCAGCATCGAATCCTGCCAGACGTACTACGTCTGCCAGTCCACCGGACCCG 179


7298R-3.IR_full       181 TGCAGTCCTCGTGCCAATCAGGCTACTCGTACGACTACAAGAGATCCTCCTGCTATCCAA 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7298R-3.in silico     181 TGCAGTCCTCGTGCCAATCAGGCTACTCGTACGACTACAAGAGATCCTCCTGCTATCCAA 239


7298R-3.IR_full       241 GCAGCGAGGTGGACTGCTATTGGGGCGTTGAGAATCCCTGTGCTGGCAAGAATAACACCT 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7298R-3.in silico     241 GCAGCGAGGTGGACTGCTATTGGGGCGTTGAGAATCCCTGTGCTGGCAAGAATAACACCT 299


7298R-3.IR_full       301 GGGTGCCAA<span class="snp"><tt>N</tt></span>ACACTGCCGTTTGTGGTGGATGGTTCTA<span class="snp"><tt>N</tt></span>CTGCCTTGAGGGCAAGTCGGC 359
                          |||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||
7298R-3.in silico     301 GGGTGCCAA<span class="snp"><tt>-</tt></span>ACACTGCCGTTTGTGGTGGATGGTTCTA<span class="snp"><tt>-</tt></span>CTGCCTTGAGGGCAAGTCGGC 359


7298R-3.IR_full       361 GGGCTCCGGAAACTGCCCGGTGAACCAGAAATTCGATACCACCACACTCGCCTGCGTTTA 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7298R-3.in silico     361 GGGCTCCGGAAACTGCCCGGTGAACCAGAAATTCGATACCACCACACTCGCCTGCGTTTA 419


7298R-3.IR_full       421 TGGAACCTGCTCCAACACTCAAGGTACTAATGAAACCGTGCTAGAATCACTATGCGATGT 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7298R-3.in silico     421 TGGAACCTGCTCCAACACTCAAGGTACTAATGAAACCGTGCTAGAATCACTATGCGATGT 479


7298R-3.IR_full       481 CGT<span class="snp"><tt>N</tt></span>CCACCTGGCCAATATT<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span> 501
                          |||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>
7298R-3.in silico     481 CGT<span class="snp"><tt>G</tt></span>CCACCTGGCCAATATT<span class="snp"><tt>T</tt></span><span class="snp"><tt>C</tt></span> 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	6	3	NM_140930.3	CG7298-RA (CG7298), mRNA
0	0	2	3	3	NM_057543.3	CG9998-RA (U2af50), mRNA
0	0	1	5	3	NM_164773.1	CG7123-RB, transcript variant B (LanB1), mRNA
0	0	1	5	3	NM_057270.3	CG7123-RA, transcript variant A (LanB1), mRNA
0	0	1	5	2	NM_168715.1	CG7930-RB, transcript variant B (TpnC73F), mRNA
0	0	1	3	7	NM_167329.2	CG17762-RC, transcript variant C (tomosyn), mRNA
0	0	1	3	3	NM_079643.1	CG5178-RA (Act88F), mRNA
0	0	1	3	2	NM_136643.2	CG8806-RA (prel), mRNA
0	0	1	3	2	NM_137780.2	CG9304-RA (CG9304), mRNA
0	0	1	3	2	NM_165446.1	CG11163-RB, transcript variant B (CG11163), mRNA
0	0	1	3	2	NM_165447.1	CG11163-RC, transcript variant C (CG11163), mRNA
0	0	1	3	2	NM_136341.2	CG11163-RA, transcript variant A (CG11163), mRNA
0	0	1	3	2	NM_165448.1	CG11163-RD, transcript variant D (CG11163), mRNA
0	0	1	2	4	NM_167330.2	CG17762-RD, transcript variant D (tomosyn), mRNA
0	0	1	2	4	NM_167328.2	CG17762-RA, transcript variant A (tomosyn), mRNA
0	0	1	2	4	NM_132591.3	CG17762-RB, transcript variant B (tomosyn), mRNA
0	0	1	2	4	NM_130715.2	CG13316-RB, transcript variant B (Mnt), mRNA
0	0	1	2	2	NM_136133.2	CG10188-RB, transcript variant B (CG10188), mRNA
0	0	1	2	2	NM_165305.2	CG10188-RA, transcript variant A (CG10188), mRNA
0	0	0	7	3	NM_165245.2	CG31746-RA (CG31746), mRNA
0	0	0	4	5	NM_135705.2	CG6618-RB, transcript variant B (Patsas), mRNA
0	0	0	4	5	NM_164993.1	CG6618-RA, transcript variant A (Patsas), mRNA
0	0	0	4	4	NM_132061.1	CG5937-RA (CG5937), mRNA
0	0	0	4	2	NM_130559.2	CG14788-RA (l(1)G0431), mRNA
0	0	0	3	11	NM_132416.1	CG2111-RA (CG2111), mRNA
0	0	0	3	8	NM_001042842.1	CG41352-RA (CG41352), mRNA
0	0	0	3	7	NM_135124.1	CG14001-RA (bchs), mRNA
0	0	0	3	6	NM_166568.1	CG3499-RB (CG3499), mRNA
0	0	0	3	4	NM_142463.2	CG7678-RA (CG7678), mRNA
0	0	0	3	4	NM_170213.2	CG31102-RA (CG31102), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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