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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7290R-2 
 Symbol CG7290  Full Name CG7290 
 CG No CG7290  Old CG No CG7290 
 Synonyms CG7290 
 Accession No (Link to NCBI) NM_140931.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCTCCAGCAATAGCCGATGTG 
 Primer Seq. 3' 
aatctagaggtaccAGAACGTTGTCCTGGCAGAAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCTCCAGCAA TAGCCGATGT GAATGTCACC GCCTTGTGCC TGCTGGTCAG CAATGGGAAC 
0061 TACGTGGCCA GCCAGTCGGA CTGCTCGACC TACTACCAGT GCCAGGGATC CTCGTTCACG 
0121 GCCATGTCCT GTCCCCAGGG CTACTACTTC GACAAGAATG CCCAGCAGTG CACGGGCACT 
0181 GTGCCGAGCA CCTGCACCAG CAACTCCGAT CCCTGTCTGG GCAAGGCGGT GGGATCCTTC 
0241 GCGGCATCCA GTTCGTCCTG CGGCGGCTAC TACTACTGCG gNGGCTCCGG CGCTGTGAGG 
0301 GGCAATTGTC CTGCCGGCGA GAACTTCAAT CCCACCACAA TGGCCTGTGT GTACAAGAAC 
0361 AACTATCCAT GCAGCGAGTC CGCCGGTGAT GGCTCCACTG TGTCGGTCGC CCTCAACCTA 
0421 TGCAATCTTG TCAAGAATGG CTTTTACTTT GGAAGCCCTT CCGACTGCAG TGGCTGGAAC 
0481 TTCTGCCAGG ACAACGTTCT  
 in silico PCR Fragment 
0001 GCTCCAGCAA TAGCCGATGT GAATGTCACC GCCTTGTGCC TGCTGGTCAG CAATGGGAAC 
0061 TACGTGGCCA GCCAGTCGGA CTGCTCGACC TACTACCAGT GCCAGGGATC CTCGTTCACG 
0121 GCCATGTCCT GTCCCCAGGG CTACTACTTC GACAAGAATG CCCAGCAGTG CACGGGCACT 
0181 GTGCCGAGCA CCTGCACCAG CAACTCCGAT CCCTGTCTGG GCAAGGCGGT GGGATCCTTC 
0241 GCGGCATCCA GTTCGTCCTG CGGCGGCTAC TACTACTGCG GAGCCTCCGG CGCTGTGAGG 
0301 GGCAATTGTC CTGCCGGCGA GAACTTCAAT CCCACCACAA TGGCCTGTGT GTACAAGAAC 
0361 AACTATCCAT GCAGCGAGTC CGCCGGTGAT GGCTCCACTG TGTCGGTCGC CCTCAACCTA 
0421 TGCAATCTTG TCAAGAATGG CTTTTACTTT GGAAGCCCTT CCGACTGCAG TGGCTGGAAC 
0481 TTCTGCCAGG ACAACGTTCT  
 Assemble Data 
<PRE>

7290R-2.IR_full       1   GCTCCAGCAATAGCCGATGTGAATGTCACCGCCTTGTGCCTGCTGGTCAGCAATGGGAAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7290R-2.in silico     1   GCTCCAGCAATAGCCGATGTGAATGTCACCGCCTTGTGCCTGCTGGTCAGCAATGGGAAC 60


7290R-2.IR_full       61  TACGTGGCCAGCCAGTCGGACTGCTCGACCTACTACCAGTGCCAGGGATCCTCGTTCACG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7290R-2.in silico     61  TACGTGGCCAGCCAGTCGGACTGCTCGACCTACTACCAGTGCCAGGGATCCTCGTTCACG 120


7290R-2.IR_full       121 GCCATGTCCTGTCCCCAGGGCTACTACTTCGACAAGAATGCCCAGCAGTGCACGGGCACT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7290R-2.in silico     121 GCCATGTCCTGTCCCCAGGGCTACTACTTCGACAAGAATGCCCAGCAGTGCACGGGCACT 180


7290R-2.IR_full       181 GTGCCGAGCACCTGCACCAGCAACTCCGATCCCTGTCTGGGCAAGGCGGTGGGATCCTTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7290R-2.in silico     181 GTGCCGAGCACCTGCACCAGCAACTCCGATCCCTGTCTGGGCAAGGCGGTGGGATCCTTC 240


7290R-2.IR_full       241 GCGGCATCCAGTTCGTCCTGCGGCGGCTACTACTACTGCGG<span class="snp"><tt>N</tt></span>G<span class="snp"><tt>G</tt></span>CTCCGGCGCTGTGAGG 300
                          |||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||
7290R-2.in silico     241 GCGGCATCCAGTTCGTCCTGCGGCGGCTACTACTACTGCGG<span class="snp"><tt>A</tt></span>G<span class="snp"><tt>C</tt></span>CTCCGGCGCTGTGAGG 300


7290R-2.IR_full       301 GGCAATTGTCCTGCCGGCGAGAACTTCAATCCCACCACAATGGCCTGTGTGTACAAGAAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7290R-2.in silico     301 GGCAATTGTCCTGCCGGCGAGAACTTCAATCCCACCACAATGGCCTGTGTGTACAAGAAC 360


7290R-2.IR_full       361 AACTATCCATGCAGCGAGTCCGCCGGTGATGGCTCCACTGTGTCGGTCGCCCTCAACCTA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7290R-2.in silico     361 AACTATCCATGCAGCGAGTCCGCCGGTGATGGCTCCACTGTGTCGGTCGCCCTCAACCTA 420


7290R-2.IR_full       421 TGCAATCTTGTCAAGAATGGCTTTTACTTTGGAAGCCCTTCCGACTGCAGTGGCTGGAAC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7290R-2.in silico     421 TGCAATCTTGTCAAGAATGGCTTTTACTTTGGAAGCCCTTCCGACTGCAGTGGCTGGAAC 480


7290R-2.IR_full       481 TTCTGCCAGGACAACGTTCT 500
                          ||||||||||||||||||||
7290R-2.in silico     481 TTCTGCCAGGACAACGTTCT 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_140931.1  CG7290-RA (CG7290), mRNA 
0   0  4  3  2  NM_164582.1  CG3054-RA, transcript variant A (l(2)k05819), mRNA 
0   0  3  3  2  NM_167380.1  CG32628-RA (CG32628), mRNA 
0   0  2  3  2  NM_136670.2  CG1776-RA (CG1776), mRNA 
0   0  2  2  2  NM_137283.2  CG7848-RA (CG7848), mRNA 
0   0  1  7  4  NM_080191.2  CG4148-RA (wek), mRNA 
0   0  1  6  4  NM_134987.1  CG15431-RA (CG15431), mRNA 
0   0  1  4  3  NM_134567.2  CG1314-RA (CG1314), mRNA 
0   0  1  3  8  NM_134496.2  CG14215-RA (CG14215), mRNA 
0   0  1  3  4  NM_079371.2  CG17943-RA (comm), mRNA 
0   0  1  3  2  NM_136463.1  CG1339-RA (Gr43b), mRNA 
0   0  1  2  6  NM_132457.1  CG11122-RA (CG11122), mRNA 
0   0  1  2  5  NM_140933.1  CG7017-RA (CG7017), mRNA 
0   0  1  2  4  NM_080516.1  CG16757-RA (Spn), mRNA 
0   0  1  2  3  NM_137177.2  CG11808-RA (CG11808), mRNA 
0   0  1  2  3  NM_132762.3  CG32594-RB, transcript variant B (CG32594), mRNA 
0   0  1  2  3  NM_167424.1  CG32594-RC, transcript variant C (CG32594), mRNA 
0   0  1  2  2  NM_136337.2  CG17337-RA (CG17337), mRNA 
0   0  1  2  2  NM_130578.2  CG32809-RD, transcript variant D (CG32809), mRNA 
0   0  0  8  22  NM_142487.1  CG7709-RA (CG7709), mRNA 
0   0  0  7  6  NM_137537.2  CG15099-RA (CG15099), mRNA 
0   0  0  6  6  NM_001038781.1  CG33979-RB, transcript variant B (capt), mRNA 
0   0  0  6  6  NM_001038780.1  CG33979-RA, transcript variant A (capt), mRNA 
0   0  0  6  4  NM_137970.3  CG5411-RE, transcript variant E (Pde8), mRNA 
0   0  0  6  4  NM_166616.1  CG5411-RA, transcript variant A (Pde8), mRNA 
0   0  0  6  2  NM_139766.1  CG6619-RA (CG6619), mRNA 
0   0  0  5  13  NM_140936.2  CG17147-RA (CG17147), mRNA 
0   0  0  5  7  NM_137564.2  CG7417-RA (Tab2), mRNA 
0   0  0  5  5  NM_135343.1  CG8498-RA (CG8498), mRNA 
0   0  0  4  8  NM_168031.1  CG1921-RC, transcript variant C (sty), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
+ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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