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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7196R-2 
 Symbol CG7196  Full Name CG7196 
 CG No CG7196  Old CG No CG7196 
 Synonyms CG7196 
 Accession No (Link to NCBI) NM_135301.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgACATGAAGGAGCTGCGGGAAC 
 Primer Seq. 3'
aatctagaggtaccCTGGGATATGCGCAACGAACG 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 ACATGAAGGA GCTGCGGGAA CGAATTGACG AGGATTACCG CAAACAGCTG CTGTCCCGCA 
0061 TTCGCGCCCG GGAGTTAAAA GAAGTGGAGC GCGAACGAAT GCTGATTATC GAGGGCAAGT 
0121 CAGACGTAAT ACCAGACGAA CTAGCCAACG ATCCCATATT CATGGTTAAC AAAGATGCCA 
0181 ACATGGAGAT CCAAAAGGAG CGCTCCAAGC TGAAGACCGC TCGCGAGAAG AACGCGGAGC 
0241 GTTTGAAGCT GCAGGGCGTT AAATGGGAGC GGGAAAGGCT TCAACATGCC GCCAAGGAAG 
0301 CCGAACTGCT GGCCAAGAAA CGCGAGCGGC ACCGCCAACA GAAATTACTG GTGGAGCAGT 
0361 ACAGGACCGA GGATGCGGAG AGGGGAATGG ATCTGCTGCG CATCGATAAC GAAGACTGGC 
0421 GGGAGTGCGA AAAGAGCCTG AAGGAGTTCC TCGAACAGGA GCGTGCCAAA ATGAAGGAGC 
0481 GTTCGTTGCG CATATCCCAG  
 in silico PCR Fragment
0001 ACATGAAGGA GCTGCGGGAA CGAATTGACG AGGATTACCG CAAACAGCTG CTGTCCCGCA 
0061 TTCGCGCCCG GGAGTTAAAA GAAGTGGAGC GCGAACGAAT GCTGATTATC GAGGGCAAGT 
0121 CAGACGTAAT ACCAGACGAA CTAGCCAACG ATCCCATATT CATGGTTAAC AAAGATGCCA 
0181 ACATGGAGAT TCAAAAGGAG CGCTCCAAGC TGAAGACCGC TCGCGAGAAG AACGCGGAGC 
0241 GTTTGAAGCT GCAGGGCGTT AAATGGGAGC GGGAAAGGCT TCAACATGCC GCCAAGGAAG 
0301 CCGAACTGCT GGCCAAGAAA CGCGAGCGGC ACCGCCAACA GAAATTACTG GTGGAGCAGT 
0361 ACAGGACCGA GGATGCGGAG AGGGGAATGG ATCTGCTGCG CATCGATAAC GAAGACTGGC 
0421 GGGAGTGCGA AAAGAGCCTG AAGGAGTTCC TCGAACAGGA GCGTGCCAAA ATGAAGGAGC 
0481 GTTCGTTGCG CATATCCCAG  
 Assemble Data

7196R-2.IR_full       1   ACATGAAGGAGCTGCGGGAACGAATTGACGAGGATTACCGCAAACAGCTGCTGTCCCGCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7196R-2.in silico     1   ACATGAAGGAGCTGCGGGAACGAATTGACGAGGATTACCGCAAACAGCTGCTGTCCCGCA 60


7196R-2.IR_full       61  TTCGCGCCCGGGAGTTAAAAGAAGTGTGAGCCGCGAACGAATGCTGATTATCGAGGGCAA 120
                          |||||||||||||||||||||||||| |||| ||||||||||||||||||||||||||||
7196R-2.in silico     61  TTCGCGCCCGGGAGTTAAAAGAAGTG-GAGC-GCGAACGAATGCTGATTATCGAGGGCAA 120


7196R-2.IR_full       121 GTCAGACGTAATACCAGACGAACTAGCCAACGATCCCATATTCATGGTTAACAAAGATGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7196R-2.in silico     121 GTCAGACGTAATACCAGACGAACTAGCCAACGATCCCATATTCATGGTTAACAAAGATGC 180


7196R-2.IR_full       181 CAACATGGAGATTCAAAAGGAGCGCTCCAAGCTGAAGACCGCTCGCGAGAANGAACGCGG 240
                          |||||||||||||||||||||||||||||||||||||||||||||||||||  |||||||
7196R-2.in silico     181 CAACATGGAGATTCAAAAGGAGCGCTCCAAGCTGAAGACCGCTCGCGAGAAG-AACGCGG 240


7196R-2.IR_full       241 AGCGTTTGAAGCTGCAGGGCGTTAAATGGGAGCGGGAAAGGCTTCAACATGCCGCCAAGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7196R-2.in silico     241 AGCGTTTGAAGCTGCAGGGCGTTAAATGGGAGCGGGAAAGGCTTCAACATGCCGCCAAGG 300


7196R-2.IR_full       301 AAGCCGAACTGCTGGCCAAGAAACGCGAGCGGCACCGCCAACAGAAATTACTGGTGGAGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7196R-2.in silico     301 AAGCCGAACTGCTGGCCAAGAAACGCGAGCGGCACCGCCAACAGAAATTACTGGTGGAGC 360


7196R-2.IR_full       361 AGTACAGGACCGAGGATGCGGAGAGGGGAATGGANCTNCTGCGCATCGATAACGAAGACT 420
                          |||||||||||||||||||||||||||||||||| || ||||||||||||||||||||||
7196R-2.in silico     361 AGTACAGGACCGAGGATGCGGAGAGGGGAATGGATCTGCTGCGCATCGATAACGAAGACT 420


7196R-2.IR_full       421 GGCGGGAGTGCGAAAAGAGCCTGAAGGAGTTCCTCGAACAGGAGCGTGCCAAAATGAAGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7196R-2.in silico     421 GGCGGGAGTGCGAAAAGAGCCTGAAGGAGTTCCTCGAACAGGAGCGTGCCAAAATGAAGG 480


7196R-2.IR_full       481 AGCGTTCGTTGCGCATATCCCAG 503
                          |||||||||||||||||||||||
7196R-2.in silico     481 AGCGTTCGTTGCGCATATCCCAG 503

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
 

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