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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7160R-3

Symbol Cpr78E Full Name Cuticular protein 78E

CG No CG7160 Old CG No CG7160

Synonyms BcDNA:LP06027, Cpr78E, CG7160

Accession No (Link to NCBI) NM_141088.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.
An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.
Zoolog Sci (2016) 33(6) 583-591 [ PubMed ID = 27927092 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTAAGATTCTCATCGTCGCCC

Primer Seq. 3'

aatctagaggtaccGACCTTAGGAGGCTTAGCATA

Predicted Fragment Size

IR fragment full Seq

0001 TTAAGATTCT CATCGTCGCC CTGTCCCTTT GCACGGCCGT CGTGCTGAGT GCTCCAGTGG 
0061 ACCACGTCAC ATCCACCACC CAGCCACCGG TGGCCATTCT CGAATCATCT CACGAAAAGC 
0121 ACGAGGATGG ATCGTACAAC TTCTCTTACC TCGGCGAGGA CGGCACCCAC AGAAGGGAGG 
0181 AGGCCGTGGT CAGAAACCAA GGCACCGAGA ACGAGTACCT GGAGATCAGC GGCTCCTACT 
0241 CCTACTTCGA TGCCAACGGA CAGGAGGTGA CCGTCACCTA CAAGGCCGAT GACCACGGAT 
0301 TTGTACCAGA GGGCGGAGNA ATCCTGCCCC AGATTTCGCT GGCCGCCAAG CAGGTCAGCG 
0361 AGCAGGTGCC CCAACCAGAT CTTGACTATG CTAAGCCTCC TAAGGTC

in silico PCR Fragment

0001 TTAAGATTCT CATCGTCGCC CTGTCCCTTT GCACGGCCGT CGTGCTGAGT GCTCCAGTGG 
0061 ACCACGTCAC ATCCACCACC CAGCCACCGG TGGCCATTCT CGAATCATCT CACGAAAAGC 
0121 ACGAGGATGG ATCGTACAAC TTCTCTTACC TCGGCGAGGA CGGCACCCAC AGAAGGGAGG 
0181 AGGCCGTGGT CAGAAACCAA GGCACCGAGA ACGAGTACCT GGAGATCAGC GGCTCCTACT 
0241 CCTACTTCGA TGCCAACGGA CAGGAGGTGA CCGTCACCTA CAAGGCCGAT GACCACGGAT 
0301 TTGTACCAGA GGGCGGAGCA ATCCTGCCCC AGATTTCGCT GGCCGCCAAG CAGGTCAGCG 
0361 AGCAGGTGCC CCAACCAGAT CTTGACTATG CTAAGCCTCC TAAGGTC

Assemble Data


7160R-3.IR_full       1   TTAAGATTCTCATCGTCGCCCTGTCCCTTTGCACGGCCGTCGTGCTGAGTGCTCCAGTGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7160R-3.in silico     1   TTAAGATTCTCATCGTCGCCCTGTCCCTTTGCACGGCCGTCGTGCTGAGTGCTCCAGTGG 60


7160R-3.IR_full       61  ACCACGTCACATCCACCACCCAGCCACCGGTGGCCATTCTCGAATCATCTCACGAAAAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7160R-3.in silico     61  ACCACGTCACATCCACCACCCAGCCACCGGTGGCCATTCTCGAATCATCTCACGAAAAGC 120


7160R-3.IR_full       121 ACGAGGATGGATCGTACAACTTCTCTTACCTCGGCGAGGACGGCACCCACAGAAGGGAGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7160R-3.in silico     121 ACGAGGATGGATCGTACAACTTCTCTTACCTCGGCGAGGACGGCACCCACAGAAGGGAGG 180


7160R-3.IR_full       181 AGGCCGTGGTCAGAAACCAAGGCACCGAGAACGAGTACCTGGAGATCAGCGGCTCCTACT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7160R-3.in silico     181 AGGCCGTGGTCAGAAACCAAGGCACCGAGAACGAGTACCTGGAGATCAGCGGCTCCTACT 240


7160R-3.IR_full       241 CCTACTTCGATGCCAACGGACAGGAGGTGACCGTCACCTACAAGGCCGATGACCACGGAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7160R-3.in silico     241 CCTACTTCGATGCCAACGGACAGGAGGTGACCGTCACCTACAAGGCCGATGACCACGGAT 300


7160R-3.IR_full       301 TTGTACCAGAGGGCGGAGNAATCCTGCCCCAGATTTCGCTGGCCGCCAAGCAGGTCAGCG 360
                          |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
7160R-3.in silico     301 TTGTACCAGAGGGCGGAGCAATCCTGCCCCAGATTTCGCTGGCCGCCAAGCAGGTCAGCG 360


7160R-3.IR_full       361 AGCAGGTGCCCCAACCAGATCTTGACTATGCTAAGCCTCCTAAGGTC 407
                          |||||||||||||||||||||||||||||||||||||||||||||||
7160R-3.in silico     361 AGCAGGTGCCCCAACCAGATCTTGACTATGCTAAGCCTCCTAAGGTC 407

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	389	0	0	1	NM_141088.1	CG7160-RA (CG7160), mRNA
0	0	2	4	2	NM_166535.1	CG4444-RA (px), mRNA
0	0	2	3	4	NM_167943.1	CG32304-RA (CG32304), mRNA
0	0	2	3	3	NM_136557.2	CG8635-RA (CG8635), mRNA
0	0	2	3	3	NM_136172.2	CG10680-RA (CG10680), mRNA
0	0	1	4	4	NM_136516.2	CG8708-RA, transcript variant A (CG8708), mRNA
0	0	1	4	4	NM_165594.1	CG8708-RB, transcript variant B (CG8708), mRNA
0	0	1	4	3	NM_141779.2	CG6621-RA (CG6621), mRNA
0	0	1	2	3	NM_135156.2	CG13991-RA (CG13991), mRNA
0	0	0	6	4	NM_080273.2	CG18817-RA, transcript variant A (Tsp42Ea), mRNA
0	0	0	6	4	NM_144389.1	CG18817-RB, transcript variant B (Tsp42Ea), mRNA
0	0	0	6	4	NM_142648.2	CG5494-RA (CG5494), mRNA
0	0	0	6	4	NM_165502.1	CG18817-RC, transcript variant C (Tsp42Ea), mRNA
0	0	0	6	3	NM_132575.2	CG2560-RA (CG2560), mRNA
0	0	0	5	4	NM_144403.1	CG18777-RA (CG18777), mRNA
0	0	0	5	2	NM_137795.1	CG6613-RA (CG6613), mRNA
0	0	0	4	6	NM_137047.2	CG6197-RA (CG6197), mRNA
0	0	0	4	5	NM_133141.1	CG7992-RA (CG7992), mRNA
0	0	0	4	4	NM_165675.1	CG11804-RA, transcript variant A (ced-6), mRNA
0	0	0	4	3	NM_138195.2	CG12169-RA (CG12169), mRNA
0	0	0	4	3	NM_132622.1	CG18646-RA (CG18646), mRNA
0	0	0	4	3	NM_057469.3	CG10718-RA (neb), mRNA
0	0	0	3	117	NM_001015110.1	CG40323-PB.3 (CG40323), mRNA
0	0	0	3	8	NM_142935.1	CG13601-RA (CG13601), mRNA
0	0	0	3	8	NM_164681.1	CG9175-RB, transcript variant B (CG9175), mRNA
0	0	0	3	8	NM_135151.2	CG9175-RA, transcript variant A (CG9175), mRNA
0	0	0	3	7	NM_164944.1	CG31869-RA (CG31869), mRNA
0	0	0	3	6	NM_141666.2	CG9492-RA (CG9492), mRNA
0	0	0	3	6	NM_001038817.1	CG4551-RD, transcript variant D (smi35A), mRNA
0	0	0	3	6	NM_001038818.1	CG4551-RE, transcript variant E (smi35A), mRNA

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