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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7140R-1

Symbol

CG7140

Full Name

CG7140

CG No

CG7140

Old CG No

CG7140

Synonyms

CG7140

Accession No (Link to NCBI)

NM_141119.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAATGAGGAGGCGTATTCCATC

Primer Seq. 3'

aatctagaggtaccCAGTTGCACAGCGAGGTTTGA

Predicted Fragment Size

IR fragment full Seq

0001 AATGAGGAGG CGTATTCCAT CGTGGTCTTC GGAGCCTCAg GCGGATTGGC CAAAAAGAAG 
0061 GTGTTTCCGG CGCTTTGGGC TCTCTTCCGG GAGAATCGGC TGCCCCAGGG CACCAAAATA 
0121 TTTACTTTCA CCCGGTCCCC GCTGCAGACG AAGACGTACC GGTTGCAGAT TCTTCCCTAT 
0181 ATGGAGCTGG ACAAGCATCG GGACCCCAAG AAGTACAACC TCTTTTGGAC GACCGTGCAC 
0241 TGTGTGCAGG GCGAGTACGA CAAGCCGGAG AACTACGTGG CACTCACGGA GGCGATGGTA 
0301 CACCAGGAGA CAAAGCACAA TCAGGTGCGC GCGAATCGAA TCTTCTANCC TGGCGCTGCC 
0361 ACCGATCGTT TTCGATCAGG TGACCCTCAA TGTCAGTCGA AAATGCAGCT CGACCACGGG 
0421 ATGGAACCGC ATAATAGTGG AGAAGCCCTT CGCGCGGGAC GACATCTCCT ACAAGGCCTT 
0481 TCAAACCTCG CTGTGCAACT G

in silico PCR Fragment

0001 AATGAGGAGG CGTATTCCAT CGTGGTCTTC GGAGCCTCAG GCGGATTGGC CAAAAAGAAG 
0061 GTGTTTCCGG CGCTTTGGGC TCTCTTCCGG GAGAATCGGC TGCCCCAGGG CACCAAAATA 
0121 TTTACTTTCA CCCGGTCCCC GCTGCAGACG AAGACGTACC GGTTGCAGAT TCTTCCCTAT 
0181 ATGGAGCTGG ACAAGCATCG GGACCCCAAG AAGTACAACC TCTTTTGGAC GACCGTGCAC 
0241 TGTGTGCAGG GCGAGTACGA CAAGCCGGAG AACTACGTGG CACTCACGGA GGCGATGGTA 
0301 CACCAGGAGA CAAAGCACAA TCAGGTGCGC GCGAATCGAA TCTTCTA-CC TGGCGCTGCC 
0361 ACCGATCGTT TTCGATCAGG TGACCCTCAA TGTCAGTCGA AAATGCAGCT CGACCACGGG 
0421 ATGGAACCGC ATAATAGTGG AGAAGCCCTT CGCGCGGGAC GACATCTCCT ACAAGGCCTT 
0481 TCAAACCTCG CTGTGCAACT G

Assemble Data

<PRE>

7140R-1.IR_full       1   AATGAGGAGGCGTATTCCATCGTGGTCTTCGGAGCCTCAGGCGGATTGGCCAAAAAGAAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7140R-1.in silico     1   AATGAGGAGGCGTATTCCATCGTGGTCTTCGGAGCCTCAGGCGGATTGGCCAAAAAGAAG 60


7140R-1.IR_full       61  GTGTTTCCGGCGCTTTGGGCTCTCTTCCGGGAGAATCGGCTGCCCCAGGGCACCAAAATA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7140R-1.in silico     61  GTGTTTCCGGCGCTTTGGGCTCTCTTCCGGGAGAATCGGCTGCCCCAGGGCACCAAAATA 120


7140R-1.IR_full       121 TTTACTTTCACCCGGTCCCCGCTGCAGACGAAGACGTACCGGTTGCAGATTCTTCCCTAT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7140R-1.in silico     121 TTTACTTTCACCCGGTCCCCGCTGCAGACGAAGACGTACCGGTTGCAGATTCTTCCCTAT 180


7140R-1.IR_full       181 ATGGAGCTGGACAAGCATCGGGACCCCAAGAAGTACAACCTCTTTTGGACGACCGTGCAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7140R-1.in silico     181 ATGGAGCTGGACAAGCATCGGGACCCCAAGAAGTACAACCTCTTTTGGACGACCGTGCAC 240


7140R-1.IR_full       241 TGTGTGCAGGGCGAGTACGACAAGCCGGAGAACTACGTGGCACTCACGGAGGCGATGGTA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7140R-1.in silico     241 TGTGTGCAGGGCGAGTACGACAAGCCGGAGAACTACGTGGCACTCACGGAGGCGATGGTA 300


7140R-1.IR_full       301 CACCAGGAGACAAAGCACAATCAGGTGCGCGCGAATCGAATCTTCTA<span class="snp"><tt>N</tt></span>CCTGGCGCTGCC 360
                          |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
7140R-1.in silico     301 CACCAGGAGACAAAGCACAATCAGGTGCGCGCGAATCGAATCTTCTA<span class="snp"><tt>-</tt></span>CCTGGCGCTGCC 360


7140R-1.IR_full       361 ACCGATCGTTTTCGATCAGGTGACCCTCAATGTCAGTCGAAAATGCAGCTCGACCACGGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7140R-1.in silico     361 ACCGATCGTTTTCGATCAGGTGACCCTCAATGTCAGTCGAAAATGCAGCTCGACCACGGG 420


7140R-1.IR_full       421 ATGGAACCGCATAATAGTGGAGAAGCCCTTCGCGCGGGACGACATCTCCTACAAGGCCTT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7140R-1.in silico     421 ATGGAACCGCATAATAGTGGAGAAGCCCTTCGCGCGGGACGACATCTCCTACAAGGCCTT 480


7140R-1.IR_full       481 TCAAACCTCGCTGTGCAACTG 501
                          |||||||||||||||||||||
7140R-1.in silico     481 TCAAACCTCGCTGTGCAACTG 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_141119.1	CG7140-RA (CG7140), mRNA
0	0	3	4	7	NM_143505.2	CG7920-RA, transcript variant A (CG7920), mRNA
0	0	1	5	2	NM_167540.1	CG32570-RA (CG32570), mRNA
0	0	1	4	9	NM_132543.1	CG10362-RA (CG10362), mRNA
0	0	1	4	2	NM_079109.2	CG5575-RA (ken), mRNA
0	0	1	3	4	NM_130606.2	CG3573-RA (CG3573), mRNA
0	0	1	3	3	NM_140327.1	CG10522-RA (sti), mRNA
0	0	1	3	2	NM_166144.1	CG8428-RC, transcript variant C (spin), mRNA
0	0	1	3	2	NM_166146.1	CG8428-RB, transcript variant B (spin), mRNA
0	0	1	3	2	NM_132080.2	CG3455-RA (Rpt4), mRNA
0	0	0	6	24	NM_080495.3	CG12047-RC, transcript variant C (mud), mRNA
0	0	0	6	24	NM_167400.3	CG12047-RA, transcript variant A (mud), mRNA
0	0	0	6	24	NM_167399.4	CG12047-RB, transcript variant B (mud), mRNA
0	0	0	5	8	NM_132792.2	CG6227-RA (CG6227), mRNA
0	0	0	4	8	NM_141607.1	CG11033-RA (CG11033), mRNA
0	0	0	4	6	NM_078735.4	CG15396-RA, transcript variant A (Gr23a), mRNA
0	0	0	4	5	NM_057425.2	CG9450-RA (tud), mRNA
0	0	0	4	5	NM_079107.2	CG11173-RA (usnp), mRNA
0	0	0	4	5	NM_164544.2	CG8851-RB, transcript variant B (CG8851), mRNA
0	0	0	4	5	NM_134944.2	CG8851-RA, transcript variant A (CG8851), mRNA
0	0	0	4	3	NM_176104.1	CG33087-RC (CG33087), mRNA
0	0	0	4	3	NM_057828.3	CG17903-RA (Cyt-c-p), mRNA
0	0	0	4	3	NM_001015117.1	CG40241-PA.3 (CG40241), mRNA
0	0	0	4	2	NM_078889.3	CG12548-RB, transcript variant B (nompB), mRNA
0	0	0	4	2	NM_165385.2	CG12548-RA, transcript variant A (nompB), mRNA
0	0	0	3	16	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	0	3	8	NM_139711.2	CG10626-RA (Lkr), mRNA
0	0	0	3	7	NM_141742.2	CG6312-RA (Rfx), mRNA
0	0	0	3	6	NM_166161.1	CG30100-RA (CG30100), mRNA
0	0	0	3	6	NM_168366.2	CG32046-RB, transcript variant B (CG32046), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00030 : Pentose phosphate pathway
dme00480 : Glutathione metabolism
dme01100 : Metabolic pathways
dme01200 : Carbon metabolism

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