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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7139R-3

Symbol

CG7139

Full Name

CG7139

CG No

CG7139

Old CG No

CG7139

Synonyms

BcDNA:LD21293, anon-WO0118547.415, CG7139

Accession No (Link to NCBI)

NM_141121.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

larval lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGTCGACAGAGCGAAGCTCG

Primer Seq. 3'

aatctagaggtaccTGGTTCCACTCGTGGGCAGCT

Predicted Fragment Size

IR fragment full Seq

0001 ATGTCGACAG AGCNAAGCTc GCATTCAGCT GAACCAGCGG ATTTGGCGCT TTTGTTGGAC 
0061 CGCATCAACA TCAACAACAA CAATAATAAT AATAAGAATA ATAATAATAA TAATAACAAC 
0121 AACAACAACA ACAATAATAA TAATGACAAT AACAATTGCG GTCGCAGCAA GAGCGGTGGA 
0181 ACTCACAGGA AATATGCAAC TGCTGAAGCC CCAAGCTCCA TTAGAATGTG CCCCGCCAGC 
0241 AATCTGAACA GCAACCAAAG CAACAAACAA TTTAACTCGA TTTGCCAGCG GGCGCAGGCG 
0301 GGCCATAAGC TGATGATCAT AATGCGCGGC CCTTCAGGCA GCGGTAAGAG TACACTGGCA 
0361 GAGTCTCTGC TGCGCCAGGC GCATCTACTC GATCGACACC AGGTGCGCGA CTTTGTCCTG 
0421 AGCTCAGACG ACTACTTCAA GACNGCGCcg CGGCTTAATG TCTTCAACCC CACTCTGCTG 
0481 CCAGCTGCCC ACGAGTGGAA CCNAGGTA

in silico PCR Fragment

0001 ATGTCGACAG AGCGAAGCTC GCATTCAGCT GAACCAGCGG ATTTGGCGCT TTTGTTGGAC 
0061 CGCATCAACA TCAACAACAA CAATAATAAT AATAAGAATA ATAATAATAA TAATAACAAC 
0121 AACAACAACA ACAATAATAA TAATGACAAT AACAATTGCG GTCGCAGCAA GAGCGGTGGA 
0181 ACTCACAGGA AATATGCAAC TGCTGAAGCC CCAAGCTCCA TTAGAATGTG CCCCGCCAGC 
0241 AATCTGAACA GCAACCAAAG CAACAAACAA TTTAACTCGA TTTGCCAGCG GGCGCAGGCG 
0301 GGCCATAAGC TGATGATCAT AATGCGCGGC CCTTCAGGCA GCGGTAAGAG TACACTGGCA 
0361 GAGTCTCTGC TGCGCCAGGC GCATCTACTC GATCGACACC AGGTGCGCGA CTTTGTCCTG 
0421 AGCTCAGACG ACTACTTCAA GAC-GCGCCG CGGCT--ATG TCTTCAACCC CACTCTGCTG 
0481 CCAGCTGCCC ACGAGTGGAA CC-----A

Assemble Data

<PRE>

7139R-3.IR_full       1   ATGTCGACAGAGC<span class="snp"><tt>N</tt></span>AAGCTCGCATTCAGCTGAACCAGCGGATTTGGCGCTTTTGTTGGAC 60
                          |||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||
7139R-3.in silico     1   ATGTCGACAGAGC<span class="snp"><tt>G</tt></span>AAGCTCGCATTCAGCTGAACCAGCGGATTTGGCGCTTTTGTTGGAC 60


7139R-3.IR_full       61  CGCATCAACATCAACAACAACAATAATAATAATAAGAATAATAATAATAATAATAACAAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7139R-3.in silico     61  CGCATCAACATCAACAACAACAATAATAATAATAAGAATAATAATAATAATAATAACAAC 120


7139R-3.IR_full       121 AACAACAACAACAATAATAATAATGACAATAACAATTGCGGTCGCAGCAAGAGCGGTGGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7139R-3.in silico     121 AACAACAACAACAATAATAATAATGACAATAACAATTGCGGTCGCAGCAAGAGCGGTGGA 180


7139R-3.IR_full       181 ACTCACAGGAAATATGCAACTGCTGAAGCCCCAAGCTCCATTAGAATGTGCCCCGCCAGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7139R-3.in silico     181 ACTCACAGGAAATATGCAACTGCTGAAGCCCCAAGCTCCATTAGAATGTGCCCCGCCAGC 240


7139R-3.IR_full       241 AATCTGAACAGCAACCAAAGCAACAAACAATTTAACTCGATTTGCCAGCGGGCGCAGGCG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7139R-3.in silico     241 AATCTGAACAGCAACCAAAGCAACAAACAATTTAACTCGATTTGCCAGCGGGCGCAGGCG 300


7139R-3.IR_full       301 GGCCATAAGCTGATGATCATAATGCGCGGCCCTTCAGGCAGCGGTAAGAGTACACTGGCA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7139R-3.in silico     301 GGCCATAAGCTGATGATCATAATGCGCGGCCCTTCAGGCAGCGGTAAGAGTACACTGGCA 360


7139R-3.IR_full       361 GAGTCTCTGCTGCGCCAGGCGCATCTACTCGATCGACACCAGGTGCGCGACTTTGTCCTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7139R-3.in silico     361 GAGTCTCTGCTGCGCCAGGCGCATCTACTCGATCGACACCAGGTGCGCGACTTTGTCCTG 420


7139R-3.IR_full       421 AGCTCAGACGACTACTTCAAGAC<span class="snp"><tt>N</tt></span>GCGCCGCGGCT<span class="snp"><tt>T</tt></span><span class="snp"><tt>A</tt></span>ATGTCTTCAACCCCACTCTGCTG 480
                          |||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
7139R-3.in silico     421 AGCTCAGACGACTACTTCAAGAC<span class="snp"><tt>-</tt></span>GCGCCGCGGCT<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>ATGTCTTCAACCCCACTCTGCTG 480


7139R-3.IR_full       481 CCAGCTGCCCACGAGTGGAACC<span class="snp"><tt>N</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>T</tt></span>A 508
                          ||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|
7139R-3.in silico     481 CCAGCTGCCCACGAGTGGAACC<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>A 508

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
101.65	490	108	264	361	NM_141121.2	CG7139-RA, transcript variant A (CG7139), mRNA
7.88	38	117	227	279	NM_140928.2	CG32223-RA (CG32223), mRNA
7.67	37	317	202	370	NM_141228.1	CG12001-RA (CG12001), mRNA
6.43	31	99	225	312	NM_078516.2	CG12690-RA (CHES-1-like), mRNA
6.22	30	81	136	233	NM_166999.2	CG32790-RA (CG32790), mRNA
4.77	23	79	207	442	NM_168571.2	CG32133-RA (CG32133), mRNA
4.35	21	79	182	293	NM_168240.1	CG32365-RA (CG32365), mRNA
4.14	20	82	196	369	NM_001038965.1	CG9924-RE, transcript variant E (CG9924), mRNA
4.14	20	82	192	359	NM_142068.1	CG9924-RC, transcript variant C (CG9924), mRNA
4.14	20	82	192	359	NM_169566.1	CG9924-RB, transcript variant B (CG9924), mRNA
4.14	20	82	192	359	NM_169565.1	CG9924-RA, transcript variant A (CG9924), mRNA
4.14	20	82	192	359	NM_169564.1	CG9924-RD, transcript variant D (CG9924), mRNA
4.14	20	47	125	226	NM_134554.1	CG15455-RA (CG15455), mRNA
3.94	19	57	126	237	NM_176712.1	CG33181-RA (CG33181), mRNA
3.52	17	107	267	379	NM_079002.2	CG3905-RA (Su(z)2), mRNA
3.11	15	95	213	339	NM_135259.2	CG4502-RA, transcript variant A (CG4502), mRNA
3.11	15	95	213	339	NM_164741.1	CG4502-RB, transcript variant B (CG4502), mRNA
2.9	14	92	290	531	NM_168239.1	CG17888-RC, transcript variant C (Pdp1), mRNA
2.9	14	30	41	73	NM_142931.3	CG10192-RA (CG10192), mRNA
2.69	13	60	155	300	NM_165585.1	CG12769-RB, transcript variant B (CG12769), mRNA
2.69	13	38	82	157	NM_138988.2	CG11405-RA (A3-3), mRNA
2.69	13	33	89	245	NM_057368.3	CG2621-RD, transcript variant D (sgg), mRNA
2.48	12	47	102	135	NM_134301.3	CG1759-RB, transcript variant B (cad), mRNA
2.48	12	47	102	135	NM_057606.4	CG1759-RA, transcript variant A (cad), mRNA
2.28	11	89	139	198	NM_001042805.1	CG34144-RA (CG34144), mRNA
2.28	11	45	96	112	NM_132908.2	CG4453-RA (Nup153), mRNA
2.28	11	36	81	173	NM_132068.1	CG15899-RB (Ca-alpha1T), mRNA
2.28	11	35	79	114	NM_079109.2	CG5575-RA (ken), mRNA
2.28	11	33	67	134	NM_169817.1	CG14307-RG, transcript variant G (fru), mRNA
2.28	11	33	67	134	NM_169818.1	CG14307-RH, transcript variant H (fru), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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