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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7130R-1 
 Symbol CG7130  Full Name CG7130 
 CG No CG7130  Old CG No CG7130 
 Synonyms CG7130 
 Accession No (Link to NCBI) NM_141123.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGATTCTGGGCATCGAGAGGA 
 Primer Seq. 3' 
aatctagaggtaccATCGCCGTGGGTAGCCTCTTT 
 Predicted Fragment Size 
350 
 IR fragment full Seq 
0001 AGATTCTGGG CATCGAGAGG AATGCGTCCA GCGAAGACGT CAAGAAGGGA TACCGCCGGA 
0061 TGGNTCTCCG CTACCATCCG GACAAGAACG ACCATCCGCA GGCCGAGGAG CAGTTTAGGG 
0121 AGGTGGTGGC CGCCTTCGAA GTGCTCTTTG ATAAGGAAAA GCGCGAGATA TACGACCAGC 
0181 ACGGCGAGNA AGGGTCTCAA ATGTGATGAC GAGCCTGCTG CGACCTTCNG CCCNAGCCCA 
0241 CGCCAGACAT GCTCCCCTTC ATGTGCGCCG TCGGAGGAAC CGTGCTCTTT GCGTTCGCCG 
0301 CCTACAAGAC ATTCCAGTTC TTCAACCGGA AAAAAGAGGC TACCCACGGC GAT 
 in silico PCR Fragment 
0001 AGATTCTGGG CATCGAGAGG AATGCGTCCA GCGAAGACGT CAAGAAGGGA TACCGCCGGA 
0061 TGGCTCTCCG CTACCATCCG GACAAGAACG ACCATCCGCA GGCCGAGGAG CAGTTTAGGG 
0121 AGGTGGTGGC CGCCTTCGAA GTGCTCTTTG ATAAGGAAAA GCGCGAGATA TACGACCAGC 
0181 ACGGCGAG-G AGGGTCTCAA ATGTGATGAC GAGCCTGCTG CGACCTTC-G CCC-AGCCCA 
0241 CGCCAGACAT GCTCCCCTTC ATGTGCGCCG TCGGAGGAAC CGTGCTCTTT GCGTTCGCCG 
0301 CCTACAAGAC ATTCCAGTTC TTCAACCGGA AAAAAGAGGC TACCCACGGC GAT 
 Assemble Data 
<PRE>

7130R-1.IR_full       1   AGATTCTGGGCATCGAGAGGAATGCGTCCAGCGAAGACGTCAAGAAGGGATACCGCCGGA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7130R-1.in silico     1   AGATTCTGGGCATCGAGAGGAATGCGTCCAGCGAAGACGTCAAGAAGGGATACCGCCGGA 60


7130R-1.IR_full       61  TGG<span class="snp"><tt>N</tt></span>TCTCCGCTACCATCCGGACAAGAACGACCATCCGCAGGCCGAGGAGCAGTTTAGGG 120
                          |||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7130R-1.in silico     61  TGG<span class="snp"><tt>C</tt></span>TCTCCGCTACCATCCGGACAAGAACGACCATCCGCAGGCCGAGGAGCAGTTTAGGG 120


7130R-1.IR_full       121 AGGTGGTGGCCGCCTTCGAAGTGCTCTTTGATAAGGAAAAGCGCGAGATATACGACCAGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7130R-1.in silico     121 AGGTGGTGGCCGCCTTCGAAGTGCTCTTTGATAAGGAAAAGCGCGAGATATACGACCAGC 180


7130R-1.IR_full       181 ACGGCGAG<span class="snp"><tt>N</tt></span><span class="snp"><tt>A</tt></span>AGGGTCTCAAATGTGATGACGAGCCTGCTGCGACCTTC<span class="snp"><tt>N</tt></span>GCCC<span class="snp"><tt>N</tt></span>AGCCCA 240
                          ||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>||||||
7130R-1.in silico     181 ACGGCGAG<span class="snp"><tt>-</tt></span><span class="snp"><tt>G</tt></span>AGGGTCTCAAATGTGATGACGAGCCTGCTGCGACCTTC<span class="snp"><tt>-</tt></span>GCCC<span class="snp"><tt>-</tt></span>AGCCCA 240


7130R-1.IR_full       241 CGCCAGACATGCTCCCCTTCATGTGCGCCGTCGGAGGAACCGTGCTCTTTGCGTTCGCCG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7130R-1.in silico     241 CGCCAGACATGCTCCCCTTCATGTGCGCCGTCGGAGGAACCGTGCTCTTTGCGTTCGCCG 300


7130R-1.IR_full       301 CCTACAAGACATTCCAGTTCTTCAACCGGAAAAAAGAGGCTACCCACGGCGAT 353
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||
7130R-1.in silico     301 CCTACAAGACATTCCAGTTCTTCAACCGGAAAAAAGAGGCTACCCACGGCGAT 353

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   332  0  0  0  NM_141123.1  CG7130-RA (CG7130), mRNA 
0   0  5  6  3  NM_132405.2  CG2887-RA (CG2887), mRNA 
0   0  3  2  3  NM_165059.1  CG7578-RB, transcript variant B (sec71), mRNA 
0   0  3  2  3  NM_135831.2  CG7578-RA, transcript variant A (sec71), mRNA 
0   0  2  3  8  NM_169522.1  CG8863-RE, transcript variant E (CG8863), mRNA 
0   0  2  3  8  NM_169519.1  CG8863-RB, transcript variant B (CG8863), mRNA 
0   0  2  3  8  NM_169520.1  CG8863-RC, transcript variant C (CG8863), mRNA 
0   0  2  3  8  NM_169521.1  CG8863-RD, transcript variant D (CG8863), mRNA 
0   0  2  3  8  NM_142026.2  CG8863-RA, transcript variant A (CG8863), mRNA 
0   0  2  3  3  NM_164409.2  CG4896-RC, transcript variant C (CG4896), mRNA 
0   0  2  3  3  NM_164410.2  CG4896-RA, transcript variant A (CG4896), mRNA 
0   0  2  3  3  NM_134739.5  CG4896-RD, transcript variant D (CG4896), mRNA 
0   0  2  3  3  NM_164411.2  CG4896-RB, transcript variant B (CG4896), mRNA 
0   0  1  5  3  NM_142072.2  CG9922-RA (CG9922), mRNA 
0   0  1  3  3  NM_164879.1  CG31876-RA (CG31876), mRNA 
0   0  1  2  13  NM_134742.2  CG5001-RA (CG5001), mRNA 
0   0  1  2  6  NM_001031864.1  CG33950-RE, transcript variant E (trol), mRNA 
0   0  1  2  6  NM_001031866.1  CG33950-RA, transcript variant A (trol), mRNA 
0   0  1  2  6  NM_001031863.1  CG33950-RD, transcript variant D (trol), mRNA 
0   0  1  2  6  NM_001031862.1  CG33950-RC, transcript variant C (trol), mRNA 
0   0  1  2  6  NM_001031867.1  CG33950-RF, transcript variant F (trol), mRNA 
0   0  1  2  5  NM_001031865.1  CG33950-RB, transcript variant B (trol), mRNA 
0   0  1  2  5  NM_001038838.1  CG40176-RA (CG40176), mRNA 
0   0  1  2  4  NM_079263.2  CG5263-RA, transcript variant A (smg), mRNA 
0   0  1  2  4  NM_168310.1  CG5263-RC, transcript variant C (smg), mRNA 
0   0  1  2  4  NM_168309.1  CG5263-RB, transcript variant B (smg), mRNA 
0   0  1  2  4  NM_137736.1  CG15666-RA (CG15666), mRNA 
0   0  1  2  2  NM_136563.1  CG14746-RA (PGRP-SC1a), mRNA 
0   0  1  2  2  NM_136565.1  CG8577-RA (PGRP-SC1b), mRNA 
0   0  1  2  2  NM_141232.1  CG9769-RA (CG9769), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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