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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7108-2R-1 |
| Symbol |
DNApol-alpha50 |
Full Name |
DNA polymerase alpha 50kD |
| CG No |
CG7108 |
Old CG No |
CG7108 |
| Synonyms |
Dmpol alpha, DNApolalpha50, CG7108, pol alpha, DNA polalpha, polalpha50, Dpri50, polalpha, PRI1, Polalpha, POLA, DNApolalpha, DNApol-alpha, DNApol-alpha50, anon-WO0140519.257, Pol |
| Accession No (Link to NCBI) |
NM_079248.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGCGTGGCGAACTAGAATCTT |
| Primer Seq. 3' |
aatctagaggtaccTCAAAACTGCAAGCTGGCAGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CGCGTgGCGA NACTAGAATC TTATATGCAA AAGAATTTGG AGGATGGAGC ACACTCGCGA
0061 CTCGTTTGGG AGAGCTTCAT TAAGTACGCC AACTCTATGA GAACATCCAC GACAAGTGCC
0121 TGGAGTCGCA AGCTGAAGAA CATTGTAGCC GAAATCCAGC TTGGACTGCT CTATCCTCGT
0181 TTGGACATCA ATGTCACTAG GGGATTCAAT CATTTGCTAA AGGCACCGTT TTGCATTCAT
0241 CCAGCCACTG GAAAAGTGTG CGTTCCGTTT AGTGTGAGTG CTGTGGCCAA GTTTGATCCC
0301 ACCACAGTGC CGACGATCAC CCAGTTGCTT CACGAGATCA ACGCTTTCGA TGATAAGAGC
0361 AAGAGCTACA TGGAGGCGCC GGAGGATAAG AGCCGCATCA AGGATCACAA GAAGACTAGC
0421 ATGTTCAAGG GTGTCGTGGT TTTCGAGGAG TTCCTGCGAA AACTGGAGCG CAGCCAGNAA
0481 ATCTGCCAGC TTGCAGTTTT GA |
| in silico PCR Fragment |
0001 CGCGTGGCGA -ACTAGAATC TTATATGCAA AAGAATTTGG AGGATGGAGC ACACTCGCGA
0061 CTCGTTTGGG AGAGCTTCAT TAAGTACGCC AACTCTATGA GAACATCCAC GACAAGTGCC
0121 TGGAGTCGCA AGCTGAAGAA CATTGTAGCC GAAATCCAGC TTGGACTGCT CTATCCTCGT
0181 TTGGACATCA ATGTCACTAG GGGATTCAAT CATTTGCTAA AGGCACCGTT TTGCATTCAT
0241 CCAGCCACTG GAAAAGTGTG CGTTCCGTTT AGTGTGAGTG CTGTGGCCAA GTTTGATCCC
0301 ACCACAGTGC CGACGATCAC CCAGTTGCTT CACGAGATCA ACGCTTTCGA TGATAAGAGC
0361 AAGAGCTACA TGGAGGCGCC GGAGGATAAG AGCCGCATCA AGGATCACAA GAAGACTAGC
0421 ATGTTCAAGG GTGTCGTGGT TTTCGAGGAG TTCCTGCGAA AACTGGAGCG CAGCCAG-AA
0481 ATCTGCCAGC TTGCAGTTTT GA |
| Assemble Data |
<PRE>
7108-2R-1.IR_full 1 CGCGTGGCGA<span class="snp"><tt>N</tt></span>ACTAGAATCTTATATGCAAAAGAATTTGGAGGATGGAGCACACTCGCGA 60
||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
7108-2R-1.in silico 1 CGCGTGGCGA<span class="snp"><tt>-</tt></span>ACTAGAATCTTATATGCAAAAGAATTTGGAGGATGGAGCACACTCGCGA 60
7108-2R-1.IR_full 61 CTCGTTTGGGAGAGCTTCATTAAGTACGCCAACTCTATGAGAACATCCACGACAAGTGCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7108-2R-1.in silico 61 CTCGTTTGGGAGAGCTTCATTAAGTACGCCAACTCTATGAGAACATCCACGACAAGTGCC 120
7108-2R-1.IR_full 121 TGGAGTCGCAAGCTGAAGAACATTGTAGCCGAAATCCAGCTTGGACTGCTCTATCCTCGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7108-2R-1.in silico 121 TGGAGTCGCAAGCTGAAGAACATTGTAGCCGAAATCCAGCTTGGACTGCTCTATCCTCGT 180
7108-2R-1.IR_full 181 TTGGACATCAATGTCACTAGGGGATTCAATCATTTGCTAAAGGCACCGTTTTGCATTCAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7108-2R-1.in silico 181 TTGGACATCAATGTCACTAGGGGATTCAATCATTTGCTAAAGGCACCGTTTTGCATTCAT 240
7108-2R-1.IR_full 241 CCAGCCACTGGAAAAGTGTGCGTTCCGTTTAGTGTGAGTGCTGTGGCCAAGTTTGATCCC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7108-2R-1.in silico 241 CCAGCCACTGGAAAAGTGTGCGTTCCGTTTAGTGTGAGTGCTGTGGCCAAGTTTGATCCC 300
7108-2R-1.IR_full 301 ACCACAGTGCCGACGATCACCCAGTTGCTTCACGAGATCAACGCTTTCGATGATAAGAGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7108-2R-1.in silico 301 ACCACAGTGCCGACGATCACCCAGTTGCTTCACGAGATCAACGCTTTCGATGATAAGAGC 360
7108-2R-1.IR_full 361 AAGAGCTACATGGAGGCGCCGGAGGATAAGAGCCGCATCAAGGATCACAAGAAGACTAGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7108-2R-1.in silico 361 AAGAGCTACATGGAGGCGCCGGAGGATAAGAGCCGCATCAAGGATCACAAGAAGACTAGC 420
7108-2R-1.IR_full 421 ATGTTCAAGGGTGTCGTGGTTTTCGAGGAGTTCCTGCGAAAACTGGAGCGCAGCCAG<span class="snp"><tt>N</tt></span>AA 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||
7108-2R-1.in silico 421 ATGTTCAAGGGTGTCGTGGTTTTCGAGGAGTTCCTGCGAAAACTGGAGCGCAGCCAG<span class="snp"><tt>-</tt></span>AA 480
7108-2R-1.IR_full 481 ATCTGCCAGCTTGCAGTTTTGA 502
||||||||||||||||||||||
7108-2R-1.in silico 481 ATCTGCCAGCTTGCAGTTTTGA 502
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079248.2 |
CG7108-RA (DNApol-alpha50), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_206244.1 |
CG33232-RC, transcript variant C (CG33232), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_206246.1 |
CG33232-RA, transcript variant A (CG33232), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_206245.1 |
CG33232-RB, transcript variant B (CG33232), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_001031933.1 |
CG33232-RD, transcript variant D (CG33232), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_137084.2 |
CG8241-RA (CG8241), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_079821.2 |
CG1954-RA (Pkc98E), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_136091.3 |
CG10473-RA, transcript variant A (CG10473), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_165273.1 |
CG10473-RB, transcript variant B (CG10473), mRNA |
| 0 |
0 |
1 |
8 |
7 |
NM_078876.4 |
CG10697-RA, transcript variant A (Ddc), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_165280.1 |
CG10697-RB, transcript variant B (Ddc), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_165279.1 |
CG10697-RC, transcript variant C (Ddc), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_057982.3 |
CG2819-RA (Pph13), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_135412.1 |
CG9487-RA (CG9487), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_176356.1 |
CG9614-RF, transcript variant F (pip), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142484.1 |
CG7705-RA, transcript variant A (CG7705), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_136958.4 |
CG12372-RA (spt4), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_080015.2 |
CG5462-RD, transcript variant D (scrib), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001014670.2 |
CG5462-RH, transcript variant H (scrib), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170277.1 |
CG5462-RC, transcript variant C (scrib), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001014669.1 |
CG5462-RI, transcript variant I (scrib), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170275.1 |
CG5462-RA, transcript variant A (scrib), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001043296.1 |
CG5462-RG, transcript variant G (scrib), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170276.1 |
CG5462-RB, transcript variant B (scrib), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137964.1 |
CG5372-RA (alphaPS5), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169113.1 |
CG1213-RB, transcript variant B (CG1213), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141342.1 |
CG1213-RA, transcript variant A (CG1213), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169114.1 |
CG1213-RC, transcript variant C (CG1213), mRNA |
| 0 |
0 |
0 |
9 |
3 |
NM_130703.2 |
CG16781-RA (CG16781), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_166554.1 |
CG3612-RA (blw), mRNA |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
dme03030
:
DNA replication
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