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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7081R-2 
 Symbol CG7081  Full Name CG7081 
 CG No CG7081  Old CG No CG7081 
 Synonyms CG7081 
 Accession No (Link to NCBI) NM_139953.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.<br> In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.<br> PLoS One (2011) 6(9) e24168 [ PubMed ID = 21931660 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTTGAACAAGGACATTGCCCG 
 Primer Seq. 3' 
aatctagaggtaccTTCCATAAATCCTCCCCACAG 
 Predicted Fragment Size 
499 
 IR fragment full Seq 
0001 TTTGAACAAG GACATTGCCC GCCTGATACG CGATAATCTG CTGGAGAACT TGCAAGCCAT 
0061 CTCACCCGTT TTGTTCATCA AAATCCAACC GGAGCTCGAC TTGATCATTC AATCCGCAAT 
0121 TTGGTTTGGT TCCGTCGGCA AGCGGTGCTC CACTTTTGGC CAACAGCTAC TGGTTCTGGC 
0181 TTACGATGCC GAAAAGCTGA CCGTTTCCAG GCTGGTGCTC CATTTCATCC TGACCGTCTT 
0241 GCCGGGCTAC GTGAAGAGCT GGGAGGAGCG ACGCTTAACA AGGCGCGTAG AATGGTTTAG 
0301 CGAGGCGATT ATGTGGGTGG AGAACAGTGC CCTGATACTG AACATCCTCA ATTACTTTCG 
0361 GTTCCTGAAG ACAGGACGAA AACCCACTTT GGTGGACTAC TTATTGGGAT TGGACTACAT 
0421 TAGTCTGCGG AACAATCAAA GGAGAGACAT TGGCTACAAG TACCTAACGA GGGAGCTGCT 
0481 GTGGGGAGGA TTTATGGAA 
 in silico PCR Fragment 
0001 TTTGAACAAG GACATTGCCC GCCTGATACG CGATAATCTG CTGGAGAACT TGCAAGCCAT 
0061 CTCACCCGTT TTGTTCATCA AAATCCAACC GGAGCTCGAC TTGATCATTC AATCCGCAAT 
0121 TTGGTTTGGT TCCGTCGGCA AGCGGTGCTC CACTTTTGGC CAACAGCTAC TGGTTCTGGC 
0181 TTACGATGCC GAAAAGCTGA CCGTTTCCAG GCTGGTGCTC CATTTCATCC TGACCGTCTT 
0241 GCCGGGCTAC GTGAAGAGCT GGGAGGAGCG ACGCTTAACA AGGCGCGTAG AATGGTTTAG 
0301 CGAGGCGATT ATGTGGGTGG AGAACAGTGC CCTGATACTG AACATCCTCA ATTACTTTCG 
0361 GTTCCTGAAG ACAGGACGAA AACCCACTTT GGTGGACTAC TTATTGGGAT TGGACTACAT 
0421 TAGTCTGCGG AACAATCAAA GGAGAGACAT TGGCTACAAG TACCTAACGA GGGAGCTGCT 
0481 GTGGGGAGGA TTTATGGAA 
 Assemble Data 
<PRE>

7081R-2.IR_full       1   TTTGAACAAGGACATTGCCCGCCTGATACGCGATAATCTGCTGGAGAACTTGCAAGCCAT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7081R-2.in silico     1   TTTGAACAAGGACATTGCCCGCCTGATACGCGATAATCTGCTGGAGAACTTGCAAGCCAT 60


7081R-2.IR_full       61  CTCACCCGTTTTGTTCATCAAAATCCAACCGGAGCTCGACTTGATCATTCAATCCGCAAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7081R-2.in silico     61  CTCACCCGTTTTGTTCATCAAAATCCAACCGGAGCTCGACTTGATCATTCAATCCGCAAT 120


7081R-2.IR_full       121 TTGGTTTGGTTCCGTCGGCAAGCGGTGCTCCACTTTTGGCCAACAGCTACTGGTTCTGGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7081R-2.in silico     121 TTGGTTTGGTTCCGTCGGCAAGCGGTGCTCCACTTTTGGCCAACAGCTACTGGTTCTGGC 180


7081R-2.IR_full       181 TTACGATGCCGAAAAGCTGACCGTTTCCAGGCTGGTGCTCCATTTCATCCTGACCGTCTT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7081R-2.in silico     181 TTACGATGCCGAAAAGCTGACCGTTTCCAGGCTGGTGCTCCATTTCATCCTGACCGTCTT 240


7081R-2.IR_full       241 GCCGGGCTACGTGAAGAGCTGGGAGGAGCGACGCTTAACAAGGCGCGTAGAATGGTTTAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7081R-2.in silico     241 GCCGGGCTACGTGAAGAGCTGGGAGGAGCGACGCTTAACAAGGCGCGTAGAATGGTTTAG 300


7081R-2.IR_full       301 CGAGGCGATTATGTGGGTGGAGAACAGTGCCCTGATACTGAACATCCTCAATTACTTTCG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7081R-2.in silico     301 CGAGGCGATTATGTGGGTGGAGAACAGTGCCCTGATACTGAACATCCTCAATTACTTTCG 360


7081R-2.IR_full       361 GTTCCTGAAGACAGGACGAAAACCCACTTTGGTGGACTACTTATTGGGATTGGACTACAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7081R-2.in silico     361 GTTCCTGAAGACAGGACGAAAACCCACTTTGGTGGACTACTTATTGGGATTGGACTACAT 420


7081R-2.IR_full       421 TAGTCTGCGGAACAATCAAAGGAGAGACATTGGCTACAAGTACCTAACGAGGGAGCTGCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7081R-2.in silico     421 TAGTCTGCGGAACAATCAAAGGAGAGACATTGGCTACAAGTACCTAACGAGGGAGCTGCT 480


7081R-2.IR_full       481 GTGGGGAGGATTTATGGAA 499
                          |||||||||||||||||||
7081R-2.in silico     481 GTGGGGAGGATTTATGGAA 499

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   481  0  0  0  NM_139953.2  CG7081-RA (CG7081), mRNA 
0.62   3  2  4  2  NM_169478.1  CG6148-RA, transcript variant A (Past1), mRNA 
0.62   3  2  4  2  NM_079608.2  CG6148-RB, transcript variant B (Past1), mRNA 
0   0  2  2  2  NM_001042978.1  CG17484-RB, transcript variant B (p120ctn), mRNA 
0   0  2  2  2  NM_001042979.1  CG17484-RA, transcript variant A (p120ctn), mRNA 
0   0  1  5  4  NM_137159.2  CG10247-RA (Cyp6a21), mRNA 
0   0  1  3  5  NM_057575.2  CG8704-RA (dpn), mRNA 
0   0  1  3  3  NM_166703.1  CG2727-RC, transcript variant C (emp), mRNA 
0   0  1  3  3  NM_166702.1  CG2727-RB, transcript variant B (emp), mRNA 
0   0  1  3  3  NM_079135.2  CG2727-RA, transcript variant A (emp), mRNA 
0   0  1  2  4  NM_058085.4  CG9765-RC, transcript variant C (tacc), mRNA 
0   0  1  2  3  NM_130698.2  CG10793-RA (CG10793), mRNA 
0   0  1  2  2  NM_136639.1  CG8800-RA (CG8800), mRNA 
0   0  1  2  2  NM_079210.1  CG10642-RA (Klp64D), mRNA 
0   0  0  5  6  NM_001043114.1  CG33484-RD, transcript variant D (zormin), mRNA 
0   0  0  5  4  NM_134509.1  CG12534-RA (CG12534), mRNA 
0   0  0  5  4  NM_142503.2  CG14291-RA (CG14291), mRNA 
0   0  0  4  5  NM_001042973.1  CG40129-RA (Gprk1), mRNA 
0   0  0  4  5  NM_134488.2  CG14211-RB (CG14211), mRNA 
0   0  0  4  3  NM_132068.1  CG15899-RB (Ca-alpha1T), mRNA 
0   0  0  4  3  NM_136465.2  CG2065-RA (CG2065), mRNA 
0   0  0  4  3  NM_143178.1  CG5886-RA (CG5886), mRNA 
0   0  0  4  3  NM_136711.2  CG11866-RA (CG11866), mRNA 
0   0  0  3  6  NM_078684.2  CG3291-RA (pcm), mRNA 
0   0  0  3  5  NM_176560.1  CG33096-RB, transcript variant B (CG33096), mRNA 
0   0  0  3  5  NM_165220.1  CG6605-RA (BicD), mRNA 
0   0  0  3  5  NM_165738.1  CG2269-RC, transcript variant C (CG2269), mRNA 
0   0  0  3  5  NM_079283.3  CG14176-RB (Or67b), mRNA 
0   0  0  3  5  NM_136709.2  CG2269-RA, transcript variant A (CG2269), mRNA 
0   0  0  3  5  NM_165737.1  CG2269-RB, transcript variant B (CG2269), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme04146 : Peroxisome

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