National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7076R-3 
 Symbol Cpr66Cb  Full Name Cuticular protein 66Cb 
 CG No CG7076  Old CG No CG7076 
 Synonyms CG7076, Cpr66Cb 
 Accession No (Link to NCBI) NM_139952.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.<br> An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.<br> Zoolog Sci (2016) 33(6) 583-591 [ PubMed ID = 27927092 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATCTTCGCCTCGGCTCTGTG 
 Primer Seq. 3' 
aatctagaggtaccTGGTGTTCGTGCAGCTCCTCA 
 Predicted Fragment Size 
465 
 IR fragment full Seq 
0001 GATCTNTCGC CTCGGCTCTG TGCCTGAGCC TCGCCTATCC GTTGGAGCAC CAGTACTACG 
0061 AGGAGAGCCA TGGATATGAG CACCTGGCCC ATCACGAACC GGAGCCCATC CATGAGTATG 
0121 GCCACCATCA AATCGAACGC ATCTCACTGG GCGAGGAGCA CGGTCACCTC GAACACGCTG 
0181 AGCCCCACTA CGAGACCCAC GAGTCTCACG GACATGATGA ACATGTGGAT TACTATGCTC 
0241 CTCCCAAATA TGCTTTCAAG TACGGCGTGA ATGACTTCCA CACCGGAGAT GTGAAGTCGC 
0301 AGCACGAGAC CCGCGATGGT GACACCGTCA AGGGACAGTA CTCCCTGGTG GAGCCCGATG 
0361 GTTCCATCCG CACCGTGGAC TACACCGCCG ACAAGCACAA TGGATTCAAC GCCGTGGTCC 
0421 ACAAGACCGC TCCAGTGCAT CATCATGAGG AGCTGCACGA ACACCA 
 in silico PCR Fragment 
0001 GATCT-TCGC CTCGGCTCTG TGCCTGAGCC TCGCCTATCC GTTGGAGCAC CAGTACTACG 
0061 AGGAGAGCCA TGGATATGAG CACCTGGCCC ATCACGAACC GGAGCCCATC CATGAGTATG 
0121 GCCACCATCA AATCGAACGC ATCTCACTGG GCGAGGAGCA CGGTCACCTC GAACACGCTG 
0181 AGCCCCACTA CGAGACCCAC GAGTCTCACG GACATGATGA ACATGTGGAT TACTATGCTC 
0241 CTCCCAAATA TGCTTTCAAG TACGGCGTGA ATGACTTCCA CACCGGAGAT GTGAAGTCGC 
0301 AGCACGAGAC CCGCGATGGT GACACCGTCA AGGGACAGTA CTCCCTGGTG GAGCCCGATG 
0361 GTTCCATCCG CACCGTGGAC TACACCGCCG ACAAGCACAA TGGATTCAAC GCCGTGGTCC 
0421 ACAAGACCGC TCCAGTGCAT CATCATGAGG AGCTGCACGA ACACCA 
 Assemble Data 
<PRE>

7076R-3.IR_full       1   GATCT<span class="snp"><tt>N</tt></span>TCGCCTCGGCTCTGTGCCTGAGCCTCGCCTATCCGTTGGAGCACCAGTACTACG 60
                          |||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||
7076R-3.in silico     1   GATCT<span class="snp"><tt>-</tt></span>TCGCCTCGGCTCTGTGCCTGAGCCTCGCCTATCCGTTGGAGCACCAGTACTACG 60


7076R-3.IR_full       61  AGGAGAGCCATGGATATGAGCACCTGGCCCATCACGAACCGGAGCCCATCCATGAGTATG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7076R-3.in silico     61  AGGAGAGCCATGGATATGAGCACCTGGCCCATCACGAACCGGAGCCCATCCATGAGTATG 120


7076R-3.IR_full       121 GCCACCATCAAATCGAACGCATCTCACTGGGCGAGGAGCACGGTCACCTCGAACACGCTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7076R-3.in silico     121 GCCACCATCAAATCGAACGCATCTCACTGGGCGAGGAGCACGGTCACCTCGAACACGCTG 180


7076R-3.IR_full       181 AGCCCCACTACGAGACCCACGAGTCTCACGGACATGATGAACATGTGGATTACTATGCTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7076R-3.in silico     181 AGCCCCACTACGAGACCCACGAGTCTCACGGACATGATGAACATGTGGATTACTATGCTC 240


7076R-3.IR_full       241 CTCCCAAATATGCTTTCAAGTACGGCGTGAATGACTTCCACACCGGAGATGTGAAGTCGC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7076R-3.in silico     241 CTCCCAAATATGCTTTCAAGTACGGCGTGAATGACTTCCACACCGGAGATGTGAAGTCGC 300


7076R-3.IR_full       301 AGCACGAGACCCGCGATGGTGACACCGTCAAGGGACAGTACTCCCTGGTGGAGCCCGATG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7076R-3.in silico     301 AGCACGAGACCCGCGATGGTGACACCGTCAAGGGACAGTACTCCCTGGTGGAGCCCGATG 360


7076R-3.IR_full       361 GTTCCATCCGCACCGTGGACTACACCGCCGACAAGCACAATGGATTCAACGCCGTGGTCC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7076R-3.in silico     361 GTTCCATCCGCACCGTGGACTACACCGCCGACAAGCACAATGGATTCAACGCCGTGGTCC 420


7076R-3.IR_full       421 ACAAGACCGCTCCAGTGCATCATCATGAGGAGCTGCACGAACACCA 466
                          ||||||||||||||||||||||||||||||||||||||||||||||
7076R-3.in silico     421 ACAAGACCGCTCCAGTGCATCATCATGAGGAGCTGCACGAACACCA 466

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   447  0  0  0  NM_139952.1  CG7076-RA (CG7076), mRNA 
0.44   2  32  19  36  NM_139411.1  CG1919-RA (CG1919), mRNA 
0.44   2  2  5  2  NM_141343.2  CG14676-RA (CG14676), mRNA 
0   0  21  31  30  NM_139951.1  CG7072-RA (CG7072), mRNA 
0   0  8  39  43  NM_139410.1  CG13935-RA (CG13935), mRNA 
0   0  5  9  17  NM_079523.1  CG2345-RA (Edg84A), mRNA 
0   0  4  5  12  NM_132038.1  CG4052-RA (CG4052), mRNA 
0   0  4  4  2  NM_143701.2  CG6698-RA (NtR), mRNA 
0   0  3  4  2  NM_079642.2  CG6177-RA (ldlCp), mRNA 
0   0  3  3  12  NM_057558.3  CG16963-RA (Cry), mRNA 
0   0  3  3  4  NM_135689.2  CG12264-RA (CG12264), mRNA 
0   0  2  4  3  NM_176278.2  CG33160-RA (CG33160), mRNA 
0   0  2  3  3  NM_057737.2  CG15804-RA, transcript variant A (Dhc62B), mRNA 
0   0  2  3  3  NM_206236.1  CG15804-RB, transcript variant B (Dhc62B), mRNA 
0   0  2  2  3  NM_138759.2  CG9242-RA (bur), mRNA 
0   0  1  8  22  NM_142556.2  CG6240-RA (CG6240), mRNA 
0   0  1  7  3  NM_142283.2  CG17565-RA (CG17565), mRNA 
0   0  1  5  8  NM_141422.2  CG1330-RA (Ccp84Ae), mRNA 
0   0  1  4  10  NM_143061.1  CG13648-RA (tnc), mRNA 
0   0  1  4  5  NM_164879.1  CG31876-RA (CG31876), mRNA 
0   0  1  4  4  NM_170365.1  CG14066-RC, transcript variant C (larp), mRNA 
0   0  1  4  4  NM_170366.1  CG14066-RB, transcript variant B (larp), mRNA 
0   0  1  4  4  NM_080259.1  CG14066-RA, transcript variant A (larp), mRNA 
0   0  1  4  2  NM_079175.2  CG1242-RA (Hsp83), mRNA 
0   0  1  3  22  NM_140863.1  CG9283-RA (CG9283), mRNA 
0   0  1  3  5  NM_142322.1  CG18622-RA (CG18622), mRNA 
0   0  1  3  3  NM_057389.4  CG1391-RA, transcript variant A (sol), mRNA 
0   0  1  3  3  NM_206802.1  CG1391-RC, transcript variant C (sol), mRNA 
0   0  1  3  3  NM_057390.4  CG1391-RB, transcript variant B (sol), mRNA 
0   0  1  2  3  NM_167623.1  CG6394-RB, transcript variant B (GalNAc-T2), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.