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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7020R-3

Symbol

DIP2

Full Name

DISCO Interacting Protein 2

CG No

CG7020

Old CG No

CG7020

Synonyms

CG7020, Ddip2, unnamed, DIP2

Accession No (Link to NCBI)

NM_138175.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.<br> DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.<br> Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCTAAAGAAGCCAGAAGGCGA

Primer Seq. 3'

aatctagaggtaccATGGCATTGGAAGGCTGGGTT

Predicted Fragment Size

IR fragment full Seq

0001 CCTAAAGAAG CCAGAAgGNN NTAAAGTGAA AAGTACGCCG CCACCGCCAT ACTACAACGT 
0061 TAAAAATGCC AACAACAGCA CCAACCACGG AAACATCAAT AACGACGGCG TCATTGTGTC 
0121 CAGCGAGGGC TACAGTTATG TGACTGAAGT GCCCTCCCTG TCCTCCTCGC AGCAAAGACA 
0181 TTCCAAAAAA ATTGACTTCC ATCAGCAAGC CGCCATGAGC TTGTCATCGG CACCACAGAG 
0241 CGGAAATGCT GGGGCTCCTG GCTACGAAAA TATGCGACCG CAGGGTGGAG CAGTCGGCGA 
0301 TCCCGGGTAT CAGAACACTC GCGAACCAAG TGCTTTTCAG AACCAACAGT CAACTAATAA 
0361 TAGTCAGCAT CGTCAACGAC GCACACAGCG CAAAGTAACG CACAATGAGA AACGTTATCA 
0421 TTCTGAAGTA CGACAAGAGG CTGTGCAACA GGCCCTTGCA GCTCTTAAGG GACGGCCAAA 
0481 ACCCAGCCTT CCAATGCCAT

in silico PCR Fragment

0001 CCTAAAGAAG CCAGAAGGCG ATAAAGTGAA AAGTACGCCG CCACCGCCAT ACTACAACGT 
0061 TAAAAATGCC AACAACAGCA CCAACCACGG AAACATCAAT AACGACGGCG TCATTGTGTC 
0121 CAGCGAGGGC TACAGTTATG TGACTGAAGT GCCCTCCCTG TCCTCCTCGC AGCAAAGACA 
0181 TTCCAAAAAA ATTGACTTCC ATCAGCAAGC CGCCATGAGC TTGTCATCGG CACCACAGAG 
0241 CGGAAATGCT GGGGCTCCTG GCTACGAAAA TATGCGACCG CAGGGTGGAG CAGTCGGCGA 
0301 TCCCGGGTAT CAGAACACTC GCGAACCAAG TGCTTTTCAG AACCAACAGT CAACTAATAA 
0361 TAGTCAGCAT CGTCAACGAC GCACACAGCG CAAAGTAACG CACAATGAGA AACGTTATCA 
0421 TTCTGAAGTA CGACAAGAGG CTGTGCAACA GGCCCTTGCA GCTCTTAAGG GACGGCCAAA 
0481 ACCCAGCCTT CCAATGCCAT

Assemble Data

<PRE>

7020R-3.IR_full       1   CCTAAAGAAGCCAGAAGG<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>TAAAGTGAAAAGTACGCCGCCACCGCCATACTACAACGT 60
                          ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||
7020R-3.in silico     1   CCTAAAGAAGCCAGAAGG<span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span>TAAAGTGAAAAGTACGCCGCCACCGCCATACTACAACGT 60


7020R-3.IR_full       61  TAAAAATGCCAACAACAGCACCAACCACGGAAACATCAATAACGACGGCGTCATTGTGTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7020R-3.in silico     61  TAAAAATGCCAACAACAGCACCAACCACGGAAACATCAATAACGACGGCGTCATTGTGTC 120


7020R-3.IR_full       121 CAGCGAGGGCTACAGTTATGTGACTGAAGTGCCCTCCCTGTCCTCCTCGCAGCAAAGACA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7020R-3.in silico     121 CAGCGAGGGCTACAGTTATGTGACTGAAGTGCCCTCCCTGTCCTCCTCGCAGCAAAGACA 180


7020R-3.IR_full       181 TTCCAAAAAAATTGACTTCCATCAGCAAGCCGCCATGAGCTTGTCATCGGCACCACAGAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7020R-3.in silico     181 TTCCAAAAAAATTGACTTCCATCAGCAAGCCGCCATGAGCTTGTCATCGGCACCACAGAG 240


7020R-3.IR_full       241 CGGAAATGCTGGGGCTCCTGGCTACGAAAATATGCGACCGCAGGGTGGAGCAGTCGGCGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7020R-3.in silico     241 CGGAAATGCTGGGGCTCCTGGCTACGAAAATATGCGACCGCAGGGTGGAGCAGTCGGCGA 300


7020R-3.IR_full       301 TCCCGGGTATCAGAACACTCGCGAACCAAGTGCTTTTCAGAACCAACAGTCAACTAATAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7020R-3.in silico     301 TCCCGGGTATCAGAACACTCGCGAACCAAGTGCTTTTCAGAACCAACAGTCAACTAATAA 360


7020R-3.IR_full       361 TAGTCAGCATCGTCAACGACGCACACAGCGCAAAGTAACGCACAATGAGAAACGTTATCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7020R-3.in silico     361 TAGTCAGCATCGTCAACGACGCACACAGCGCAAAGTAACGCACAATGAGAAACGTTATCA 420


7020R-3.IR_full       421 TTCTGAAGTACGACAAGAGGCTGTGCAACAGGCCCTTGCAGCTCTTAAGGGACGGCCAAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7020R-3.in silico     421 TTCTGAAGTACGACAAGAGGCTGTGCAACAGGCCCTTGCAGCTCTTAAGGGACGGCCAAA 480


7020R-3.IR_full       481 ACCCAGCCTTCCAATGCCAT 500
                          ||||||||||||||||||||
7020R-3.in silico     481 ACCCAGCCTTCCAATGCCAT 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_138175.2	CG7020-RA (DIP2), mRNA
0	0	4	2	4	NM_130646.2	CG14045-RA (CG14045), mRNA
0	0	2	3	2	NM_140156.1	CG6418-RB (CG6418), mRNA
0	0	2	2	6	NM_141072.2	CG7177-RA (CG7177), mRNA
0	0	2	2	4	NM_079871.2	CG1775-RA, transcript variant A (Med), mRNA
0	0	2	2	3	NM_170559.1	CG1775-RB, transcript variant B (Med), mRNA
0	0	2	2	2	NM_141653.2	CG16789-RA (CG16789), mRNA
0	0	1	6	17	NM_078717.2	CG3696-RA, transcript variant A (kis), mRNA
0	0	1	5	14	NM_165046.1	CG9239-RB, transcript variant B (B4), mRNA
0	0	1	5	14	NM_057977.3	CG9239-RA, transcript variant A (B4), mRNA
0	0	1	5	3	NM_137729.1	CG9752-RA (CG9752), mRNA
0	0	1	4	13	NM_169136.1	CG10295-RA, transcript variant A (Pak), mRNA
0	0	1	4	9	NM_165089.2	CG3479-RA, transcript variant A (osp), mRNA
0	0	1	4	8	NM_078843.4	CG3479-RB, transcript variant B (osp), mRNA
0	0	1	3	3	NM_168624.1	CG7945-RA, transcript variant A (CG7945), mRNA
0	0	1	3	3	NM_206372.1	CG7945-RC, transcript variant C (CG7945), mRNA
0	0	1	2	12	NM_143745.2	CG18389-RA (Eip93F), mRNA
0	0	1	2	6	NM_169137.1	CG10295-RC, transcript variant C (Pak), mRNA
0	0	1	2	6	NM_079942.3	CG10295-RB, transcript variant B (Pak), mRNA
0	0	1	2	4	NM_001014605.1	CG1119-RB, transcript variant B (Gnf1), mRNA
0	0	1	2	4	NM_079505.2	CG1119-RA, transcript variant A (Gnf1), mRNA
0	0	1	2	3	NM_136041.1	CG10231-RA (Pde11), mRNA
0	0	0	7	12	NM_058003.3	CG5216-RA (Sir2), mRNA
0	0	0	6	19	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA
0	0	0	5	6	NM_165025.1	CG9828-RB, transcript variant B (DnaJ-H), mRNA
0	0	0	4	17	NM_140064.2	CG32043-RA, transcript variant A (CG32043), mRNA
0	0	0	4	17	NM_168346.1	CG32043-RB, transcript variant B (CG32043), mRNA
0	0	0	4	7	NM_135279.1	CG15819-RA (CG15819), mRNA
0	0	0	4	4	NM_206125.1	CG8295-RD, transcript variant D (Mlf), mRNA
0	0	0	4	4	NM_166122.2	CG8295-RC, transcript variant C (Mlf), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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